FastQCFastQC Report
Wed 25 May 2016
SRR1779834_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779834_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92110
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT2342325.42937791770709No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG30483.3090869612419938No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC16151.753338399739442No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT6280.681793507762458RNA PCR Primer, Index 42 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC5600.6079687330365867No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT4350.47226142655520575No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGT3870.4201498208663555No Hit
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC2480.26924329605905983RNA PCR Primer, Index 32 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT2370.2573010530886983No Hit
CTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG1910.2073607643035501No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAGCTCGTATGCCGT1780.19324720442948648No Hit
CTTTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC1420.15416350016284877No Hit
CCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG1340.14547823254804038No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCG1160.12593638041472152No Hit
TTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC1110.12050808815546629RNA PCR Primer, Index 32 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG1050.11399413744436No Hit
CTTATACACATCTCCGAGCCCACGAGACATCCCTCGATCTCGTATGCCGT980.10639452828140267No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGA150.00221839470.000011
CGTCTAT150.00221839470.0000156
ACCAAGG150.00221839470.000012
TTCGATA150.00221839470.0000138
CTTGACA207.890018E-570.057
AGCTCGT308.319301E-658.33333637
GCTCGTA308.319301E-658.33333638
ATCTCGT30750.053.7235737
TCTCGTA30700.053.69706738
CTCGTAT32400.053.5802539
TCGATCT30500.053.4754134
GATCTCG30650.053.44208536
CGATCTC30650.053.44208535
CGTCTTC32750.053.43511248
GCTTGAA32100.053.42679256
TGCTTGA32350.053.3384855
CTTGAAA31900.053.32288457
TATGCCG32900.053.29787443
ATGCCGT32900.053.29787444
CTGCTTG32600.053.25153754