##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779834_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	92110
Sequences flagged as poor quality	0
Sequence length	76
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.579253066985125	34.0	34.0	34.0	34.0	34.0
2	33.67950276842905	34.0	34.0	34.0	34.0	34.0
3	33.77711431983498	34.0	34.0	34.0	34.0	34.0
4	33.58753664097275	34.0	34.0	34.0	34.0	34.0
5	33.63846487894908	34.0	34.0	34.0	34.0	34.0
6	37.36480295299099	38.0	38.0	38.0	37.0	38.0
7	37.40242101834763	38.0	38.0	38.0	37.0	38.0
8	37.31491694712843	38.0	38.0	38.0	37.0	38.0
9	37.237856910216045	38.0	38.0	38.0	36.0	38.0
10-11	37.19031049831723	38.0	38.0	38.0	36.5	38.0
12-13	37.33171208337857	38.0	38.0	38.0	36.5	38.0
14-15	37.34260123765063	38.0	38.0	38.0	37.0	38.0
16-17	37.216431440668764	38.0	38.0	38.0	36.0	38.0
18-19	37.05009770926067	38.0	38.0	38.0	35.5	38.0
20-21	37.100293127782	38.0	38.0	38.0	35.5	38.0
22-23	37.168005645423946	38.0	38.0	38.0	36.0	38.0
24-25	37.294050591683856	38.0	38.0	38.0	36.0	38.0
26-27	37.16921615459776	38.0	37.5	38.0	36.0	38.0
28-29	36.56301161654544	38.0	37.0	38.0	34.0	38.0
30-31	36.7852784713929	38.0	37.0	38.0	35.0	38.0
32-33	36.77500814243839	38.0	37.0	38.0	35.0	38.0
34-35	36.7858918684182	38.0	37.5	38.0	35.0	38.0
36-37	36.87617522527413	38.0	37.5	38.0	35.5	38.0
38-39	35.1045163391597	38.0	36.0	38.0	29.0	38.0
40-41	36.34265009228097	38.0	37.0	38.0	34.0	38.0
42-43	37.139593963739	38.0	37.5	38.0	36.0	38.0
44-45	36.92470415807187	38.0	37.5	38.0	35.0	38.0
46-47	37.09028878514819	38.0	38.0	38.0	36.0	38.0
48-49	36.69932689175985	38.0	37.0	38.0	34.0	38.0
50-51	36.8025838671154	38.0	37.0	38.0	34.5	38.0
52-53	36.72393334057106	38.0	37.0	38.0	34.5	38.0
54-55	36.74037563782434	38.0	37.0	38.0	35.0	38.0
56-57	36.62152317880795	38.0	37.0	38.0	34.0	38.0
58-59	36.642954076647484	38.0	37.0	38.0	34.0	38.0
60-61	36.2751927043752	38.0	37.0	38.0	34.0	38.0
62-63	36.17570296384757	38.0	37.0	38.0	34.0	38.0
64-65	36.625382694604276	38.0	37.0	38.0	34.0	38.0
66-67	36.29218325914667	38.0	37.0	38.0	33.0	38.0
68-69	36.200960807729885	38.0	37.0	38.0	34.0	38.0
70-71	34.09615134078819	38.0	34.0	38.0	21.0	38.0
72-73	30.355737704918035	37.0	23.0	38.0	11.0	38.0
74-75	29.819064162414506	37.0	23.0	38.0	11.0	38.0
76	29.744870263815002	37.0	23.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	2.0
23	6.0
24	11.0
25	50.0
26	93.0
27	202.0
28	379.0
29	678.0
30	1102.0
31	1693.0
32	2563.0
33	4314.0
34	7745.0
35	16979.0
36	16634.0
37	39659.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.2297165200391	13.88508743347453	12.491582491582491	48.39361355490387
2	12.369992400390837	13.308001302790142	54.64444685701878	19.677559439800238
3	12.014982086635545	16.03083270003257	52.87156660514603	19.082618608185864
4	8.532189773097382	40.36478123982195	31.43958310715449	19.663445879926176
5	8.531104114645533	27.97416132884594	53.68038215177505	9.814352404733471
6	17.643035501031378	55.46846162197373	16.28161980241016	10.606883074584736
7	16.289219411573118	24.933232005211163	17.6647486700684	41.11279991314733
8	14.82792313538161	54.85832157203344	13.687981760938008	16.625773531646946
9	15.01465638909999	15.174248181522094	14.267723374226469	55.54337205515145
10-11	15.745847356421669	34.15698621213766	32.02909564650961	18.068070784931063
12-13	15.833242861795679	15.993920312669635	34.912604494625995	33.26023233090869
14-15	14.885463033329716	16.076430355010313	18.815546629030507	50.22255998262947
16-17	31.465096080772987	31.785365324069048	16.139398545217677	20.61014004994029
18-19	32.40907610465747	20.881554662903053	16.693084355661707	30.016284876777767
20-21	15.840299641732711	17.411790250787103	16.524264466398872	50.22364564108132
22-23	15.075453262403649	33.9642818369341	16.574747584409945	34.385517316252304
24-25	31.190763117610658	33.30293456665473	16.63735357022658	18.868948745508042
26-27	32.23862772771686	35.79307349907719	17.229942460102052	14.738356313103898
28-29	15.752361307132775	35.69644989686245	17.307024210183478	31.2441645858213
30-31	14.05276300075996	20.832700032569754	34.213983280859836	30.900553685810443
32-33	14.431114971230052	33.58321572033438	18.629356204538052	33.35631310389751
34-35	14.69818695038541	18.224405601997614	33.9365975464119	33.14080990120508
36-37	30.780588426880907	30.953750949951147	19.185756161111716	19.079904462056238
38-39	15.937466073173379	21.16925415264358	33.47844967973075	29.414830094452288
40-41	14.260666594289436	16.671371186624686	35.9613505591141	33.106611659971776
42-43	30.936380414721526	16.538378026272934	34.15807187058951	18.367169688416023
44-45	15.435891868418194	32.64846379329063	36.21593746607317	15.699706872218
46-47	30.680165020084683	17.41450439691673	22.065465204646618	29.83986537835197
48-49	29.443600043426336	15.976006948214092	19.063076756052546	35.517316252307026
50-51	17.121714723143658	16.016352412958145	35.55889746081556	31.303035403082635
52-53	13.383034870714464	16.202773584700758	51.51656657580661	18.897624968778167
54-55	12.656000521359445	16.664494335646864	38.23302594849403	32.44647919449966
56-57	29.604001803201225	16.361878588071715	22.148416496032414	31.885703112694646
58-59	14.583446463286162	17.146518674587576	49.249541144911326	19.02049371721494
60-61	29.09750389592055	31.95034941113229	22.82167815080878	16.130468542138384
62-63	14.1022647676862	52.12277853493259	17.408467223039708	16.366489474341503
64-65	12.869722897845726	54.08432505211953	16.769457956914525	16.276494093120224
66-67	12.636163211225387	54.29966223921283	16.24944340063208	16.814731148929702
68-69	12.660083278592408	54.10176926292473	16.28492787769882	16.953219580784033
70-71	13.11591999826272	51.6601880605442	16.44498251862147	18.77890942257161
72-73	14.303786472076533	44.514664842384164	17.575007329706484	23.606541355832817
74-75	14.890278727863013	41.29016146019957	18.274753792197355	25.544806019740058
76	14.710097719869708	42.12812160694897	17.995656894679694	25.166123778501632
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	0.5
15	10.5
16	21.0
17	21.0
18	35.0
19	62.5
20	81.5
21	87.0
22	110.5
23	161.0
24	225.0
25	262.0
26	328.0
27	449.5
28	539.5
29	574.0
30	680.0
31	800.0
32	1017.5
33	1221.0
34	1331.0
35	1530.5
36	1729.5
37	1839.0
38	2017.5
39	2641.5
40	6875.0
41	12378.5
42	14094.0
43	12984.0
44	9943.0
45	6666.5
46	5321.0
47	4775.0
48	3675.5
49	2941.5
50	2761.0
51	2602.0
52	2263.5
53	1905.0
54	1726.0
55	1634.0
56	1392.5
57	1230.0
58	1217.0
59	1047.5
60	852.5
61	753.0
62	679.0
63	638.5
64	533.0
65	472.0
66	431.5
67	387.0
68	363.5
69	333.0
70	320.0
71	314.0
72	297.5
73	244.5
74	194.5
75	181.0
76	141.0
77	90.0
78	83.0
79	87.0
80	75.0
81	59.0
82	52.0
83	49.0
84	41.0
85	24.5
86	12.0
87	8.0
88	5.5
89	3.5
90	2.5
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.043426338074041906
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0010856584518510477
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.014656389099989144
52-53	0.02931277819997829
54-55	0.04668331342959505
56-57	0.055911410270328955
58-59	0.03582672891108457
60-61	0.028769948974052762
62-63	0.027684290522201716
64-65	0.015199218325914667
66-67	0.03582672891108457
68-69	0.010313755292584954
70-71	0.013027901422212572
72-73	0.020627510585169907
74-75	0.01411355987406362
76	0.010856584518510477
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	92110.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.340571056345674
#Duplication Level	Percentage of deduplicated	Percentage of total
1	40.84841155402577	9.534252524155901
2	17.893855528164103	8.353056128541962
3	14.0192567096144	9.816523721637173
4	10.023722033582958	9.35837585495603
5	6.651472161495883	7.762457930734991
6	3.8745988185497002	5.4261209423515355
7	2.3070840504209498	3.7694061448268377
8	1.3209916740313503	2.4666160026055803
9	0.7814316944974186	1.641515579198784
>10	2.1675426764035537	6.977526870046684
>50	0.03721103307130564	0.6329388774291609
>100	0.05116517047304526	2.479643904027793
>500	0.00930275826782641	1.2897622407990446
>1k	0.00930275826782641	5.062425360981435
>5k	0.0	0.0
>10k+	0.004651379133913205	25.42937791770709
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT	23423	25.42937791770709	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG	3048	3.3090869612419938	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC	1615	1.753338399739442	No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT	628	0.681793507762458	RNA PCR Primer, Index 42 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC	560	0.6079687330365867	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT	435	0.47226142655520575	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGT	387	0.4201498208663555	No Hit
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC	248	0.26924329605905983	RNA PCR Primer, Index 32 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT	237	0.2573010530886983	No Hit
CTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG	191	0.2073607643035501	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAGCTCGTATGCCGT	178	0.19324720442948648	No Hit
CTTTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC	142	0.15416350016284877	No Hit
CCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG	134	0.14547823254804038	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCG	116	0.12593638041472152	No Hit
TTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC	111	0.12050808815546629	RNA PCR Primer, Index 32 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG	105	0.11399413744436	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCCCTCGATCTCGTATGCCGT	98	0.10639452828140267	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.010856584518510477	0.0
2	0.0	0.0	0.0	0.011942242970361524	0.0
3	0.0	0.0	0.0	0.011942242970361524	0.0
4	0.0	0.0	0.0	0.027141461296276193	0.0
5	0.0	0.0	0.0	0.034741070459233525	0.0
6	0.0	0.0	0.0	0.034741070459233525	0.0
7	0.0	0.0	0.0	0.034741070459233525	0.0
8	0.0	0.0	0.0	0.034741070459233525	0.0
9	0.0	0.0	0.0	0.034741070459233525	0.0
10	0.0	0.0	0.0	0.034741070459233525	0.0
11	0.0	0.0	0.0	0.03799804581478667	0.0
12	0.0	0.0	0.0	0.03799804581478667	0.0
13	0.0	0.0	0.0	0.04016936271848876	0.0
14	0.0	0.0	0.0	0.04016936271848876	0.0
15	0.0	0.0	0.0	0.04016936271848876	0.0
16	0.0	0.0	0.0	0.04016936271848876	0.0
17	0.0	0.0	0.0	0.04016936271848876	0.0
18	0.0	0.0	0.0	0.04668331342959505	0.0
19	0.0	0.0	0.0	0.05102594723699924	0.0
20	0.0	0.0	0.0	0.061882531755509715	0.0
21	0.0	0.0	0.0	0.0705677993703181	0.0
22	0.0	0.0	0.0	0.08033872543697752	0.0
23	0.0	0.0	0.0	0.08359570079253067	0.0
24	0.0	0.0	0.0	0.0890239930517859	0.0
25	0.0	0.0	0.0	0.09119530995548801	0.0
26	0.0	0.0	0.0	0.09662360221474324	0.0
27	0.0	0.0	0.0	0.11182282054065791	0.0
28	0.0	0.0	0.0	0.12593638041472152	0.0
29	0.0	0.0	0.0	0.1411355987406362	0.0
30	0.0	0.0	0.0	0.1661057431332103	0.0
31	0.0	0.0	0.0	0.2117033981109543	0.0
32	0.0	0.0	0.0	0.2616436868961025	0.0
33	0.0	0.0	0.0	0.30181304961459127	0.0
34	0.0	0.0	0.0	0.3539246553034415	0.0
35	0.0	0.0	0.0	0.4364346976441212	0.0
36	0.0	0.0	0.0	0.5884268809032678	0.0
37	0.0	0.0	0.0	0.8316143741179025	0.0
38	0.0	0.0	0.0	1.070459233525133	0.0
39	0.0	0.0	0.0	1.4298121810878297	0.0
40	0.0	0.0	0.0	1.8347627836282705	0.0
41	0.0	0.0	0.0	2.365649766583433	0.0
42	0.0	0.0	0.0	2.8813375312126803	0.0
43	0.0	0.0	0.0	3.323200521116057	0.0
44	0.0	0.0	0.0	3.78243404624905	0.0
45	0.0	0.0	0.0	4.329605905981978	0.0
46	0.0	0.0	0.0	4.923461079144501	0.0
47	0.0	0.0	0.0	5.4033221148626644	0.0
48	0.0	0.0	0.0	5.922266854847465	0.0
49	0.0	0.0	0.0	6.32178916512865	0.0
50	0.0	0.0	0.0	6.7332537183801975	0.0
51	0.0	0.0	0.0	7.220714363261318	0.0
52	0.0	0.0	0.0	7.608294430572142	0.0
53	0.0	0.0	0.0	8.074041906416241	0.0
54	0.0	0.0	0.0	8.498534361090002	0.0
55	0.0	0.0	0.0	8.917598523504505	0.0
56	0.0	0.0	0.0	9.331234393659754	0.0
57	0.0	0.0	0.0	9.72967104548909	0.0
58	0.0	0.0	0.0	10.1042232113777	0.0
59	0.0	0.0	0.0	10.41580718705895	0.0
60	0.0	0.0	0.0	10.802301595917925	0.0
61	0.0	0.0	0.0	11.172511127999131	0.0
62	0.0	0.0	0.0	11.48300944522853	0.0
63	0.0	0.0	0.0	11.902073607643036	0.0
64	0.0	0.0	0.0	12.250569970687222	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATAGA	15	0.002218394	70.00001	1
CGTCTAT	15	0.002218394	70.00001	56
ACCAAGG	15	0.002218394	70.00001	2
TTCGATA	15	0.002218394	70.00001	38
CTTGACA	20	7.890018E-5	70.0	57
AGCTCGT	30	8.319301E-6	58.333336	37
GCTCGTA	30	8.319301E-6	58.333336	38
ATCTCGT	3075	0.0	53.72357	37
TCTCGTA	3070	0.0	53.697067	38
CTCGTAT	3240	0.0	53.58025	39
TCGATCT	3050	0.0	53.47541	34
GATCTCG	3065	0.0	53.442085	36
CGATCTC	3065	0.0	53.442085	35
CGTCTTC	3275	0.0	53.435112	48
GCTTGAA	3210	0.0	53.426792	56
TGCTTGA	3235	0.0	53.33848	55
CTTGAAA	3190	0.0	53.322884	57
TATGCCG	3290	0.0	53.297874	43
ATGCCGT	3290	0.0	53.297874	44
CTGCTTG	3260	0.0	53.251537	54
>>END_MODULE