FastQCFastQC Report
Wed 25 May 2016
SRR1779833_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779833_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118213
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGG150.002218836570.0088845
CGGCCGA150.00222256569.9792718
CATTGGG150.00222256569.979271
CGTGACA150.00222256569.9792719
CTCGGTG501.4551915E-1163.00800344
TCGGTGG650.059.2382945
GCCGTAT553.45608E-1157.28000354
GTGTCGA252.3807428E-456.00711436
GTCGTTA252.3807428E-456.00711464
ACGTTGC252.3857289E-455.98341815
CGGTGGT700.055.0069846
TGGTCGC700.055.0069849
TCTCGGT900.054.4513643
CTTCCGA452.6820999E-854.42832618
ACGCTTC452.6820999E-854.42832615
GTAGATC1700.053.5362138
CCGATAT200.00693320652.50666428
CATGACG200.00693320652.50666411
TGGCCGC200.00693320652.50666449
GGGGTAC200.00693320652.50666435
Produced by FastQC (version 0.11.5)