##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779833_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	118213
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.420275265833705	32.0	21.0	34.0	12.0	34.0
2	26.819757556275537	33.0	21.0	34.0	12.0	34.0
3	26.529273430164196	32.0	21.0	34.0	11.0	34.0
4	27.027188211110452	32.0	21.0	34.0	12.0	34.0
5	26.992445839290095	34.0	21.0	34.0	11.0	34.0
6	29.3196348963312	36.0	21.0	38.0	11.0	38.0
7	29.718431982946036	36.0	21.0	38.0	12.0	38.0
8	29.015201373791378	35.0	21.0	38.0	11.0	38.0
9	29.914028067978986	36.0	23.0	38.0	11.0	38.0
10-11	28.66633111417526	34.0	21.0	38.0	11.0	38.0
12-13	29.166403018280562	35.0	21.0	38.0	11.0	38.0
14-15	28.187475150787137	34.0	21.0	38.0	11.0	38.0
16-17	28.533435408965175	35.0	21.0	38.0	11.0	38.0
18-19	29.00511788043616	35.5	21.0	38.0	11.0	38.0
20-21	28.22638796071498	33.0	16.0	38.0	11.0	38.0
22-23	28.846015243670323	35.0	21.0	38.0	11.0	38.0
24-25	29.166639878862732	35.5	21.0	38.0	11.0	38.0
26-27	28.261126102882084	34.0	21.0	38.0	11.0	38.0
28-29	28.959086564083478	35.0	21.0	38.0	11.0	38.0
30-31	29.195854093881383	37.0	21.0	38.0	11.0	38.0
32-33	29.342090125451517	37.0	21.0	38.0	11.0	38.0
34-35	29.310824528605146	36.5	21.0	38.0	11.0	38.0
36-37	28.94816982903742	35.5	21.0	38.0	11.0	38.0
38-39	28.877280840516697	35.5	21.0	38.0	11.0	38.0
40-41	28.88524527759214	35.5	21.0	38.0	11.0	38.0
42-43	29.14540701953254	36.5	21.0	38.0	11.0	38.0
44-45	29.24798034057168	36.0	21.0	38.0	11.0	38.0
46-47	29.1583497584868	36.0	21.0	38.0	11.0	38.0
48-49	28.848362701225753	35.5	21.0	38.0	11.0	38.0
50-51	28.390709989595052	35.0	21.0	38.0	11.0	38.0
52-53	28.8737956062362	35.0	21.0	38.0	11.0	38.0
54-55	28.928654208927952	35.5	21.0	38.0	11.0	38.0
56-57	29.01804370077741	36.0	21.0	38.0	11.0	38.0
58-59	29.0584199707308	36.5	21.0	38.0	11.0	38.0
60-61	29.07208598039133	36.0	21.0	38.0	11.0	38.0
62-63	29.000164956476866	36.0	21.0	38.0	11.0	38.0
64-65	28.97454594672329	36.5	21.0	38.0	11.0	38.0
66-67	29.006238738548213	36.0	21.0	38.0	11.0	38.0
68-69	28.665134122304654	34.5	21.0	38.0	11.0	38.0
70-71	28.75193506636326	34.5	21.0	38.0	11.0	38.0
72-73	28.807225093686817	35.0	21.0	38.0	11.0	38.0
74-75	28.699140534458984	35.5	21.0	38.0	11.0	38.0
76	28.04669537191341	34.0	20.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	75.0
13	882.0
14	2573.0
15	4274.0
16	5648.0
17	6606.0
18	6781.0
19	5931.0
20	4644.0
21	3406.0
22	2343.0
23	1686.0
24	1384.0
25	1119.0
26	1147.0
27	1207.0
28	1307.0
29	1489.0
30	1728.0
31	2177.0
32	2852.0
33	3567.0
34	5166.0
35	7528.0
36	13644.0
37	29045.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.74053395594356	21.635705342943186	14.465536493756979	33.15822420735628
2	15.539746051618689	27.9656213783594	32.181739740976035	24.31289282904587
3	13.843655097155136	28.311607014456953	32.437210797458825	25.407527090929083
4	11.422601575122872	28.971432921929058	27.860725977684346	31.74523952526372
5	11.631546445822371	35.529933256071665	34.613790361466165	18.224729936639793
6	20.719585134552098	42.15655587232567	18.655409578112973	18.468449415009264
7	20.618766021166977	33.112526754820095	18.985135741055643	27.283571482957285
8	17.709707982269144	40.986532670118095	19.175718201197846	22.128041146414915
9	19.53029162189829	19.777328448997896	19.06413651322747	41.62824341587635
10-11	19.003891379747905	31.34887065392099	24.107097538279334	25.54014042805177
12-13	18.95273342497621	25.516337104790104	26.01036269430052	29.520566775933172
14-15	24.75256319155416	27.899028863397962	20.40190505194058	26.9465028931073
16-17	25.083747842858596	26.65042466077894	21.04989341183636	27.215934084526104
18-19	19.337100703844072	27.00113359501895	24.855339740119113	28.80642596101787
20-21	24.399600720732913	26.0690448597024	22.3558322688706	27.175522150694086
22-23	25.11568297366573	25.84319564169155	21.74713013171363	27.293991252929082
24-25	19.429910455589443	30.822395831130322	21.164965590752015	28.582728122528223
26-27	23.364802219740806	30.3315230264271	22.185141948363956	24.118532805468142
28-29	20.09043763694661	29.485715252573964	24.300144666379026	26.123702444100406
30-31	21.087782223613306	28.502364376168444	23.671254430561785	26.73859896965647
32-33	19.118113566670193	29.394945542984036	24.26012477529872	27.226816115047054
34-35	19.039824038237843	30.92824059387095	23.861007127297338	26.17092824059387
36-37	23.243535941518882	26.405340462975495	24.217375118451333	26.133748477054286
38-39	22.851788871686843	25.050125631329156	26.372427095758994	25.72565840122501
40-41	20.71151828917538	28.827022637295705	24.472557777552197	25.988901295976717
42-43	17.62180498513258	28.88762937616053	25.67728182114261	27.813283817564283
44-45	17.374182578019916	25.947701912746286	27.594812489954062	29.08330301927974
46-47	19.716266459125535	27.193668867561406	25.572817751534355	27.517246921778703
48-49	19.893496430142456	27.97431732819003	25.30411464149156	26.828071600175956
50-51	24.443683090757588	27.060624902187204	22.628700496148817	25.866991510906388
52-53	16.56105381602213	29.160991209739507	24.92914491662366	29.3488100576147
54-55	18.13244220722912	27.284955901947928	24.228336964108205	30.354264926714748
56-57	19.112664080577684	27.873921816635853	23.726590888901107	29.286823213885356
58-59	18.009153898867183	28.69138162959704	24.307323965110278	28.992140506425496
60-61	16.548634379124785	27.460063627441027	24.575676041560904	31.415625951873288
62-63	17.924823607045568	27.811289149083773	24.135378419992893	30.12850882387777
64-65	18.29358062086996	27.451793314098605	23.9806580984694	30.273967966562033
66-67	17.288248881067407	31.49002055959321	21.615493303325916	29.60623725601347
68-69	17.39409960875542	33.24986782277678	20.464417891508933	28.891614676958866
70-71	16.811673892354868	31.656127736068523	20.720735962778893	30.81146240879772
72-73	16.48640760683363	29.921453013505683	21.02918970133534	32.562949678325346
74-75	16.483293359221328	29.40741022745058	22.954446432574883	31.15484998075321
76	15.897132222316218	30.814651890702986	21.395820996531597	31.8923948904492
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	0.5
10	0.0
11	0.0
12	0.0
13	1.0
14	1.0
15	1.0
16	4.5
17	7.0
18	6.5
19	13.5
20	35.5
21	50.0
22	55.5
23	88.0
24	171.0
25	227.0
26	260.0
27	324.0
28	411.0
29	467.0
30	663.0
31	945.0
32	1285.0
33	1539.0
34	1756.5
35	2234.0
36	2816.0
37	3138.0
38	3648.0
39	4667.0
40	5410.0
41	5982.0
42	6320.0
43	6595.0
44	7168.5
45	7735.0
46	8003.0
47	7957.5
48	7859.0
49	7395.0
50	6984.0
51	6778.0
52	6136.0
53	5347.5
54	4995.0
55	4693.5
56	3881.5
57	3109.5
58	2848.0
59	2516.5
60	2003.5
61	1637.5
62	1453.0
63	1276.5
64	1010.5
65	818.0
66	645.5
67	576.0
68	532.5
69	452.5
70	375.0
71	334.0
72	298.0
73	249.5
74	193.5
75	150.0
76	151.5
77	136.0
78	105.5
79	92.0
80	83.0
81	68.0
82	51.0
83	40.0
84	35.0
85	23.5
86	17.5
87	18.0
88	18.5
89	17.5
90	15.0
91	9.5
92	5.0
93	4.0
94	4.0
95	5.0
96	5.0
97	4.0
98	7.0
99	9.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	8.459306506052633E-4
2	0.0
3	0.0
4	0.0
5	0.0
6	0.005075583903631581
7	0.008459306506052634
8	8.459306506052633E-4
9	0.010151167807263162
10-11	0.0025377919518157904
12-13	4.2296532530263165E-4
14-15	8.459306506052633E-4
16-17	8.459306506052633E-4
18-19	0.004229653253026317
20-21	0.0
22-23	0.0016918613012105266
24-25	0.0029607572771184216
26-27	8.459306506052633E-4
28-29	0.008459306506052634
30-31	0.0
32-33	4.2296532530263165E-4
34-35	0.004652618578328948
36-37	0.01776454366271053
38-39	0.008459306506052634
40-41	8.459306506052633E-4
42-43	0.0012688959759078952
44-45	0.005075583903631581
46-47	0.0029607572771184216
48-49	8.459306506052633E-4
50-51	0.0021148266265131584
52-53	0.011843029108473686
54-55	0.008882271831355265
56-57	0.013957855734986847
58-59	0.010151167807263162
60-61	0.021148266265131586
62-63	0.009305237156657896
64-65	0.020302335614526323
66-67	0.016918613012105267
68-69	4.2296532530263165E-4
70-71	4.2296532530263165E-4
72-73	0.0029607572771184216
74-75	0.009728202481960529
76	0.0025377919518157904
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	118213.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.47521846159052
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.11017480738049	62.26218774584859
2	4.460724557063899	6.376625244262475
3	2.810883741848437	6.027255885562502
4	1.9149515344466406	5.474863170717264
5	1.2793959262897519	4.572255166521448
6	0.7728450877587493	3.314356289071422
7	0.47459552862367294	2.3745273362489745
8	0.29351543914880524	1.6783264108008424
9	0.21895304936503618	1.4084745332577635
>10	0.663960328074515	6.511128217708713
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01522675171089474	0.0
2	0.0	0.0	0.0	0.02030233561452632	0.0
3	0.0	0.0	0.0	0.021994196915736848	0.0
4	0.0	0.0	0.0	0.038912809927842115	0.0
5	0.0	0.0	0.0	0.04314246318086843	0.0
6	0.0	0.0	0.0	0.04314246318086843	0.0
7	0.0	0.0	0.0	0.04314246318086843	0.0
8	0.0	0.0	0.0	0.04314246318086843	0.0
9	0.0	0.0	0.0	0.04568025513268422	0.0
10	0.0	0.0	0.0	0.04568025513268422	0.0
11	0.0	0.0	0.0	0.04568025513268422	0.0
12	0.0	0.0	0.0	0.04568025513268422	0.0
13	0.0	0.0	0.0	0.04568025513268422	0.0
14	0.0	0.0	0.0	0.04906397773510528	0.0
15	0.0	0.0	0.0	0.04906397773510528	0.0
16	0.0	0.0	0.0	0.04906397773510528	0.0
17	0.0	0.0	0.0	0.04906397773510528	0.0
18	0.0	0.0	0.0	0.04906397773510528	0.0
19	0.0	0.0	0.0	0.05160176968692107	0.0
20	0.0	0.0	0.0	0.05413956163873686	0.0
21	0.0	0.0	0.0	0.05752328424115791	0.0
22	0.0	0.0	0.0	0.06429072944600002	0.0
23	0.0	0.0	0.0	0.06429072944600002	0.0
24	0.0	0.0	0.0	0.06852038269902633	0.0
25	0.0	0.0	0.0	0.0693663133496316	0.0
26	0.0	0.0	0.0	0.0693663133496316	0.0
27	0.0	0.0	0.0	0.07951748115689476	0.0
28	0.0	0.0	0.0	0.09305237156657897	0.0
29	0.0	0.0	0.0	0.1108169152292895	0.0
30	0.0	0.0	0.0	0.14042448800047372	0.0
31	0.0	0.0	0.0	0.15988089296439478	0.0
32	0.0	0.0	0.0	0.18779660443436846	0.0
33	0.0	0.0	0.0	0.22078789980797375	0.0
34	0.0	0.0	0.0	0.28507862925397376	0.0
35	0.0	0.0	0.0	0.3510612200011843	0.0
36	0.0	0.0	0.0	0.48218047084500015	0.0
37	0.0	0.0	0.0	0.757953862942316	0.0
38	0.0	0.0	0.0	1.1487738235219476	0.0
39	0.0	0.0	0.0	1.5362100614991583	0.0
40	0.0	0.0	0.0	2.026003908199606	0.0
41	0.0	0.0	0.0	2.5521727728760797	0.0
42	0.0	0.0	0.0	3.025048006564422	0.0
43	0.0	0.0	0.0	3.459856360975527	0.0
44	0.0	0.0	0.0	4.018170590375001	0.0
45	0.0	0.0	0.0	4.711833723871317	0.0
46	0.0	0.0	0.0	5.317520069704686	0.0
47	0.0	0.0	0.0	5.894444773417475	0.0
48	0.0	0.0	0.0	6.442607835009686	0.0
49	0.0	0.0	0.0	6.9966924111561335	0.0
50	8.459306506052634E-4	0.0	0.0	7.550776987302581	0.0
51	8.459306506052634E-4	0.0	0.0	8.086251089135713	0.0
52	8.459306506052634E-4	0.0	0.0	8.571815282583135	0.0
53	8.459306506052634E-4	0.0	0.0	9.07091436644024	0.0
54	8.459306506052634E-4	0.0	0.0	9.559016351839476	0.0
55	8.459306506052634E-4	0.0	0.0	9.986211330395134	0.0
56	8.459306506052634E-4	0.0	0.0	10.498845304661923	0.0
57	8.459306506052634E-4	0.0	0.0	10.997944388519029	0.0
58	8.459306506052634E-4	0.0	0.0	11.501273125629162	0.0
59	8.459306506052634E-4	0.0	0.0	11.843875039124292	0.0
60	8.459306506052634E-4	0.0	0.0	12.25499733531845	0.0
61	8.459306506052634E-4	0.0	0.0	12.694881273633188	0.0
62	8.459306506052634E-4	0.0	0.0	13.068782621200715	0.0
63	8.459306506052634E-4	0.0	0.0	13.51966365797332	0.0
64	8.459306506052634E-4	0.0	0.0	13.913867341155372	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCGG	15	0.0022188365	70.00888	45
CGGCCGA	15	0.002222565	69.97927	18
CATTGGG	15	0.002222565	69.97927	1
CGTGACA	15	0.002222565	69.97927	19
CTCGGTG	50	1.4551915E-11	63.008003	44
TCGGTGG	65	0.0	59.23829	45
GCCGTAT	55	3.45608E-11	57.280003	54
GTGTCGA	25	2.3807428E-4	56.007114	36
GTCGTTA	25	2.3807428E-4	56.007114	64
ACGTTGC	25	2.3857289E-4	55.983418	15
CGGTGGT	70	0.0	55.00698	46
TGGTCGC	70	0.0	55.00698	49
TCTCGGT	90	0.0	54.45136	43
CTTCCGA	45	2.6820999E-8	54.428326	18
ACGCTTC	45	2.6820999E-8	54.428326	15
GTAGATC	170	0.0	53.53621	38
CCGATAT	20	0.006933206	52.506664	28
CATGACG	20	0.006933206	52.506664	11
TGGCCGC	20	0.006933206	52.506664	49
GGGGTAC	20	0.006933206	52.506664	35
>>END_MODULE