FastQCFastQC Report
Wed 25 May 2016
SRR1779833_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779833_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118213
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT3153426.675577136186373No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG39083.3058969825653692No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC22281.884733489548527No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT8690.7351137353759739RNA PCR Primer, Index 15 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC8350.7063520932553949No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGT3890.3290670230854475No Hit
CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC3540.29945945031426324No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAAACTCGTATGCCGT3280.2774652533985264No Hit
CTTTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC2220.18779660443436846No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT2080.17595357532589478No Hit
CTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG1940.1641105462174211No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC1570.13281111214502636No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTATCTCGTATGCCGTC1460.12350587498836844No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAAGCTCGTATGCCGT1430.12096808303655267No Hit
TTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC1310.1108169152292895No Hit
CCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG1240.10489540067505265No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTAG252.8156082E-670.040221
GTCAACA150.002214900270.0402151
TAGACGT150.00222235269.98096521
GTCTAGG150.00222235269.9809652
AGAGTCA150.00222235269.9809656
GTTAAGC150.00222235269.98096533
CGAATCT150.00222235269.98096542
AGAGGAC150.00222235269.9809653
AAGCTTA150.00222235269.98096514
ACGAATC150.00222235269.98096541
CTTTATA1000.059.4838142
CATACGT308.347311E-658.3174728
ACATACG308.347311E-658.3174727
GCCGTCC252.3754817E-456.03217746
TAACGAA252.3854444E-455.98476820
CTGCCGT252.3854444E-455.98476844
ATACGTT252.3854444E-455.98476829
TACGTTA252.3854444E-455.98476830
TCTTTAT1100.054.1219941
AATCTCG43800.052.96504636
Produced by FastQC (version 0.11.5)