FastQCFastQC Report
Wed 25 May 2016
SRR1779832_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779832_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117348
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAATCG150.002219913370.000018
GCCGTAT401.3278623E-1070.054
TCGGTGG750.065.33333645
TGGTCGC453.8016879E-1062.22221849
TTACGCT308.333467E-658.33333613
CGATATG308.333467E-658.33333625
TAGTGTA553.45608E-1157.27272434
CGGTGGT800.056.87546
GTAGATC1300.056.5384638
CTTGTGT252.3821658E-455.99999633
TACGCTT252.3821658E-455.99999614
GACGAAT452.6757334E-854.44444323
GATCTCG1100.054.09090841
ATCTCGG1050.053.33333242
CTCGGTG1050.053.33333244
CGTATCA608.367351E-1152.50000456
GACGATA608.367351E-1152.50000423
GACGAAA608.367351E-1152.50000423
TTTAGAC200.006936562252.518
GACGTGG200.006936562252.514
Produced by FastQC (version 0.11.5)