FastQCFastQC Report
Wed 25 May 2016
SRR1779832_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779832_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117348
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT2252619.19589596754951No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG26632.269318607901285No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC14611.245014827691993No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT5600.4772130756382725Illumina PCR Primer Index 9 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC5540.472100078399291No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT4540.3868834577495995No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCGT3890.3314926543273No Hit
CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC2480.21133721921123497No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAGCTCGTATGCCGT1990.1695810750928861No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATATCGTATGCCGT1740.14827691993046324No Hit
CTTTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC1700.14486825510447557No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG1540.13123359580052493No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTG207.874243E-570.0443747
GCGGTCT150.00221433870.04436555
GACGTCT150.00221433870.04436547
ACGTCTT150.00221433870.04436548
CAACGAG207.907602E-569.9846620
GTCGCTC150.00222184569.9846527
GACCGAC150.00222184569.984657
TGACACG150.00222184569.9846514
TGCTACG150.00222184569.9846530
CGAGACA150.00222184569.9846523
CATGAAC252.3847475E-455.9877219
CCAACGA252.3847475E-455.9877219
ATCTCGT30750.055.1911537
TCTCGTA30750.055.1911538
GATCTCG30650.054.80038536
GCCGTCT32800.054.66877446
CTCGTAT32850.054.6455539
TATGCCG33000.054.60923443
CCGTCTT32700.054.51465647
CGTATGC33250.054.5143641