##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779832_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	117348
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.6035552374135	34.0	34.0	34.0	34.0	34.0
2	33.67180522889184	34.0	34.0	34.0	34.0	34.0
3	33.77652793400825	34.0	34.0	34.0	34.0	34.0
4	33.57210178273171	34.0	34.0	34.0	34.0	34.0
5	33.63390087602686	34.0	34.0	34.0	34.0	34.0
6	37.34233220847394	38.0	38.0	38.0	37.0	38.0
7	37.379265091863516	38.0	38.0	38.0	37.0	38.0
8	37.27617002420152	38.0	38.0	38.0	36.0	38.0
9	37.22200634011658	38.0	38.0	38.0	36.0	38.0
10-11	37.15334304802809	38.0	38.0	38.0	36.0	38.0
12-13	37.30751269727648	38.0	38.0	38.0	36.5	38.0
14-15	37.331002658758564	38.0	38.0	38.0	36.5	38.0
16-17	37.222781811364484	38.0	38.0	38.0	36.0	38.0
18-19	37.06332873163582	38.0	38.0	38.0	35.5	38.0
20-21	37.11393462180864	38.0	38.0	38.0	36.0	38.0
22-23	37.10459488018543	38.0	38.0	38.0	36.0	38.0
24-25	37.250438865596344	38.0	38.0	38.0	36.0	38.0
26-27	37.17460459488018	38.0	38.0	38.0	36.0	38.0
28-29	36.72495057436002	38.0	37.0	38.0	34.0	38.0
30-31	36.93272147799707	38.0	37.5	38.0	35.5	38.0
32-33	36.957020997375324	38.0	38.0	38.0	36.0	38.0
34-35	36.92876742679892	38.0	38.0	38.0	36.0	38.0
36-37	36.952606776425675	38.0	38.0	38.0	35.5	38.0
38-39	35.724626751201555	38.0	36.5	38.0	32.0	38.0
40-41	36.60295872106896	38.0	37.0	38.0	34.5	38.0
42-43	37.146875106520774	38.0	38.0	38.0	36.0	38.0
44-45	36.934114769744696	38.0	37.5	38.0	35.0	38.0
46-47	37.087125472952245	38.0	38.0	38.0	36.0	38.0
48-49	36.69599396666325	38.0	37.0	38.0	34.5	38.0
50-51	36.83867641544806	38.0	37.5	38.0	35.0	38.0
52-53	36.76093755326039	38.0	37.0	38.0	34.5	38.0
54-55	36.757136891979414	38.0	37.0	38.0	35.0	38.0
56-57	36.64338548590517	38.0	37.0	38.0	34.5	38.0
58-59	36.69831185874493	38.0	37.0	38.0	34.5	38.0
60-61	36.3463544329686	38.0	37.0	38.0	34.0	38.0
62-63	36.20304990285305	38.0	37.0	38.0	34.0	38.0
64-65	36.60997204894842	38.0	37.0	38.0	34.0	38.0
66-67	36.32478610628217	38.0	37.0	38.0	33.5	38.0
68-69	36.31286004022225	38.0	37.0	38.0	34.0	38.0
70-71	34.74357466680301	38.0	35.5	38.0	26.5	38.0
72-73	31.989769744691003	38.0	27.0	38.0	11.0	38.0
74-75	31.59559174421379	38.0	25.0	38.0	11.0	38.0
76	31.480902955312406	37.0	25.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	4.0
23	10.0
24	28.0
25	72.0
26	145.0
27	236.0
28	388.0
29	764.0
30	1271.0
31	1943.0
32	3023.0
33	4740.0
34	8487.0
35	17840.0
36	20436.0
37	57961.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.13923748465421	13.134292729504843	10.868230800709316	42.85823898513163
2	16.53372873845315	14.376043903602959	46.54872686368749	22.5415004942564
3	15.382452193475816	15.435286498278625	45.78348161025326	23.398779697992296
4	11.193203122336982	33.43218461328698	29.92211200872618	25.452500255649863
5	11.894535910283942	27.06309438592903	47.73068139209872	13.311688311688311
6	23.87258410880458	48.1218256808808	15.621058731294951	12.38453147901967
7	22.630977945938575	24.348945018236357	16.575484882571498	36.444592153253566
8	19.813716467259773	47.70511640590381	14.174932678869686	18.306234447966734
9	18.43320721273477	15.558850598220678	14.69901489586529	51.308927293179266
10-11	19.94154139823431	30.349899444387635	29.07846746429424	20.63009169308382
12-13	19.852063946552136	16.681579575280363	31.55741895899376	31.90893751917374
14-15	18.29302587176603	16.700327231823294	18.50521525718376	46.501431639226915
16-17	30.528854347751988	29.778948086034703	17.432338003204144	22.25985956300917
18-19	31.31838633807138	21.1047482701026	17.88100351092477	29.695861880901255
20-21	19.139226914817467	18.50052834304803	17.830299621638204	44.5299451204963
22-23	18.298991035211507	30.910198725159354	17.95088113985752	32.83992909977162
24-25	30.67667122581415	30.867132807540067	17.918301781476227	20.537894185169563
26-27	31.12792719091932	31.905102771244504	18.607049118860143	18.359920918976037
28-29	18.845655656679277	20.866567815386713	29.80664348774585	30.48113304018816
30-31	28.827930599584146	18.00328936155708	33.84718955585097	19.321590483007807
32-33	20.304564202202	18.785577939121247	30.855234004840305	30.054623853836453
34-35	17.579336673824862	41.84689981934076	21.65439547329311	18.91936803354126
36-37	30.015424208337592	33.03124041313018	18.620257695060843	18.333077683471384
38-39	18.926611446296484	21.16567815386713	30.721869993523537	29.185840406312845
40-41	17.719091931690357	18.006271943279817	32.39509833998023	31.879537785049592
42-43	29.67596241931017	17.829523424018408	31.793604465369945	20.700909691301476
44-45	18.26234788833214	29.545028462351297	33.263881787503834	18.92874186181273
46-47	29.360960561747962	18.39997273068139	22.774141868630057	29.464924838940586
48-49	28.29745713603981	17.382060197020827	20.665030507550192	33.655452159389164
50-51	18.609368607663985	17.813309695895267	32.67165552979681	30.905666166643936
52-53	16.293937882798215	17.813409711833124	44.968694191909506	20.923958213459155
54-55	15.967565217020589	17.979817788824324	34.89766075638528	31.15495623776981
56-57	28.068612068083365	17.51714022580755	23.161902650339393	31.25234505576969
58-59	17.01796636900322	18.503868204002472	42.92065386500714	21.55751156198717
60-61	27.813203300825208	29.942286708040644	22.778421878196824	19.466088112937324
62-63	16.721294705839732	44.689003780629704	19.07662275112204	19.51307876240852
64-65	15.525838042394332	46.353780459740726	18.38110579832434	19.739275699540602
66-67	15.561049423499075	46.35195328317811	17.857675667611517	20.229321625711304
68-69	16.0485778203899	46.13145839991478	17.771812080536915	20.04815169915841
70-71	16.367510440637517	44.05650728713884	18.225943918861333	21.35003835336231
72-73	16.967413632915385	38.2597874172129	19.480646783555947	25.292152166315773
74-75	17.825638578039904	35.707954419548116	20.418729917924505	26.047677084487475
76	17.91196147781992	36.880726126049346	19.472450675416543	25.734861720714193
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	5.5
16	9.5
17	8.0
18	18.0
19	38.5
20	52.5
21	56.0
22	80.0
23	126.0
24	200.5
25	253.0
26	298.5
27	429.5
28	565.0
29	615.0
30	651.5
31	801.0
32	1115.0
33	1316.0
34	1424.5
35	1807.5
36	2150.5
37	2219.0
38	2545.0
39	6611.5
40	12015.5
41	12694.0
42	11709.0
43	10157.5
44	7353.0
45	5518.0
46	4936.0
47	4766.0
48	4342.5
49	4014.0
50	3939.0
51	3758.0
52	3387.0
53	3176.0
54	3155.0
55	3172.0
56	2915.5
57	2528.0
58	2414.0
59	2246.5
60	2037.0
61	1854.0
62	1713.0
63	1580.5
64	1410.5
65	1301.5
66	1185.0
67	1140.0
68	1035.5
69	901.5
70	801.5
71	731.0
72	712.0
73	617.0
74	522.5
75	504.0
76	484.0
77	416.0
78	348.5
79	329.0
80	299.0
81	231.5
82	179.5
83	165.0
84	149.0
85	105.5
86	83.5
87	89.0
88	66.0
89	30.5
90	15.5
91	6.5
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04431264273783959
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0012782493097453728
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	4.2608310324845753E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.017043324129938302
52-53	0.03195623274363432
54-55	0.05666905273204486
56-57	0.06817329651975321
58-59	0.03877356239560964
60-61	0.03749531308586427
62-63	0.033660565156628144
64-65	0.017895490336435216
66-67	0.03877356239560964
68-69	0.008947745168217608
70-71	0.015338991716944472
72-73	0.02471281998841054
74-75	0.01448682551044756
76	0.011078160684459897
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	117348.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.85946074922453
#Duplication Level	Percentage of deduplicated	Percentage of total
1	42.41126793834524	12.239663223915192
2	18.93049075769208	10.926475099703445
3	13.978621626409968	12.10246446466919
4	10.030709265930431	11.579234413880084
5	6.584775290852182	9.501653202440604
6	3.502037441681923	6.064014725432048
7	1.925234748715526	3.8892865664519207
8	1.039390539183842	2.3997000374953132
9	0.5905628063544558	1.5338991716944472
>10	0.9330892340400401	3.74867914237993
>50	0.032480954349495064	0.643385485905171
>100	0.026575326285950513	1.7120019088523026
>500	0.005905628063544558	0.9493131540375634
>1k	0.005905628063544558	3.514333435593278
>5k	0.0	0.0
>10k+	0.002952814031772279	19.19589596754951
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT	22526	19.19589596754951	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG	2663	2.269318607901285	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC	1461	1.245014827691993	No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT	560	0.4772130756382725	Illumina PCR Primer Index 9 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC	554	0.472100078399291	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT	454	0.3868834577495995	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCGT	389	0.3314926543273	No Hit
CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC	248	0.21133721921123497	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAGCTCGTATGCCGT	199	0.1695810750928861	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATATCGTATGCCGT	174	0.14827691993046324	No Hit
CTTTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC	170	0.14486825510447557	No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG	154	0.13123359580052493	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0042608310324845754	0.0
2	0.0	0.0	0.0	0.0042608310324845754	0.0
3	0.0	0.0	0.0	0.005112997238981491	0.0
4	0.0	0.0	0.0	0.007669495858472236	0.0
5	0.0	0.0	0.0	0.016191157923441387	0.0
6	0.0	0.0	0.0	0.016191157923441387	0.0
7	0.0	0.0	0.0	0.017043324129938302	0.0
8	0.0	0.0	0.0	0.017043324129938302	0.0
9	0.0	0.0	0.0	0.022156321368919794	0.0
10	0.0	0.0	0.0	0.023860653781913623	0.0
11	0.0	0.0	0.0	0.025564986194907456	0.0
12	0.0	0.0	0.0	0.027269318607901286	0.0
13	0.0	0.0	0.0	0.0281214848143982	0.0
14	0.0	0.0	0.0	0.028973651020895115	0.0
15	0.0	0.0	0.0	0.028973651020895115	0.0
16	0.0	0.0	0.0	0.028973651020895115	0.0
17	0.0	0.0	0.0	0.028973651020895115	0.0
18	0.0	0.0	0.0	0.028973651020895115	0.0
19	0.0	0.0	0.0	0.032382315846882774	0.0
20	0.0	0.0	0.0	0.03323448205337969	0.0
21	0.0	0.0	0.0	0.03493881446637352	0.0
22	0.0	0.0	0.0	0.03493881446637352	0.0
23	0.0	0.0	0.0	0.0391996454988581	0.0
24	0.0	0.0	0.0	0.04005181170535501	0.0
25	0.0	0.0	0.0	0.04431264273783959	0.0
26	0.0	0.0	0.0	0.050277806183317994	0.0
27	0.0	0.0	0.0	0.06135596686777789	0.0
28	0.0	0.0	0.0	0.08692095306268535	0.0
29	0.0	0.0	0.0	0.12100760132256196	0.0
30	0.0	0.0	0.0	0.20025905852677506	0.0
31	0.0	0.0	0.0	0.2420152026451239	0.0
32	0.0	0.0	0.0	0.2863278453829635	0.0
33	0.0	0.0	0.0	0.3246753246753247	0.0
34	0.0	0.0	0.0	0.39114428878208407	0.0
35	0.0	0.0	0.0	0.4959607321812046	0.0
36	0.0	0.0	0.0	0.6476463169376555	0.0
37	0.0	0.0	0.0	1.0004431264273783	0.0
38	0.0	0.0	0.0	1.3285271159286907	0.0
39	0.0	0.0	0.0	1.73756689504721	0.0
40	0.0	0.0	0.0	2.191771483110066	0.0
41	0.0	0.0	0.0	2.714149367692675	0.0
42	0.0	0.0	0.0	3.2995875515560553	0.0
43	0.0	0.0	0.0	3.8730954085284792	0.0
44	0.0	0.0	0.0	4.362238811057709	0.0
45	0.0	0.0	0.0	5.003067798343389	0.0
46	0.0	0.0	0.0	5.637079455977093	0.0
47	0.0	0.0	0.0	6.193543988819579	0.0
48	0.0	0.0	0.0	6.778130006476463	0.0
49	0.0	0.0	0.0	7.225517264887344	0.0
50	0.0	0.0	0.0	7.672904523298224	0.0
51	0.0	0.0	0.0	8.146708934110508	0.0
52	0.0	0.0	0.0	8.584722364249924	0.0
53	0.0	0.0	0.0	9.120734908136482	0.0
54	0.0	0.0	0.0	9.717251252684324	0.0
55	0.0	0.0	0.0	10.253263796570883	0.0
56	0.0	0.0	0.0	10.76541568667553	0.0
57	0.0	0.0	0.0	11.210246446466918	0.0
58	0.0	0.0	0.0	11.567304086989127	0.0
59	0.0	0.0	0.0	11.91754439785936	0.0
60	0.0	0.0	0.0	12.313801683880424	0.0
61	0.0	0.0	0.0	12.66830282578314	0.0
62	0.0	0.0	0.0	13.050925452500255	0.0
63	0.0	0.0	0.0	13.407130926815967	0.0
64	0.0	0.0	0.0	13.822135869379965	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCTG	20	7.874243E-5	70.04437	47
GCGGTCT	15	0.002214338	70.044365	55
GACGTCT	15	0.002214338	70.044365	47
ACGTCTT	15	0.002214338	70.044365	48
CAACGAG	20	7.907602E-5	69.98466	20
GTCGCTC	15	0.002221845	69.98465	27
GACCGAC	15	0.002221845	69.98465	7
TGACACG	15	0.002221845	69.98465	14
TGCTACG	15	0.002221845	69.98465	30
CGAGACA	15	0.002221845	69.98465	23
CATGAAC	25	2.3847475E-4	55.98772	19
CCAACGA	25	2.3847475E-4	55.98772	19
ATCTCGT	3075	0.0	55.19115	37
TCTCGTA	3075	0.0	55.19115	38
GATCTCG	3065	0.0	54.800385	36
GCCGTCT	3280	0.0	54.668774	46
CTCGTAT	3285	0.0	54.64555	39
TATGCCG	3300	0.0	54.609234	43
CCGTCTT	3270	0.0	54.514656	47
CGTATGC	3325	0.0	54.51436	41
>>END_MODULE