FastQCFastQC Report
Wed 25 May 2016
SRR1779831_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779831_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51566
Sequences flagged as poor quality0
Sequence length76
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGATTC150.00221283670.0000134
TTGTGTA150.00221283670.0000134
CAAGTGT150.00221283670.0000133
GATCATT150.00221283670.0000158
TTCGGTG150.00221283670.0000144
CAGTACG150.00221283670.0000153
AGTGTCG150.00221283670.0000135
TTGTCAT207.857113E-570.02
AGATTTC207.857113E-570.040
CTCGGTG601.8189894E-1258.33333644
TGCCTAC308.267576E-658.33333619
TGGTCGC601.8189894E-1258.33333649
ACGTGTA553.45608E-1157.2727334
GGTCGCC750.056.050
TTGATGC252.3696036E-455.99999612
TGTCATC252.3696036E-455.9999963
TCGGCGG509.513315E-1055.99999645
AATGATT252.3696036E-455.99999633
TCTCGGT509.513315E-1055.99999643
GTATATC252.3696036E-455.99999630
Produced by FastQC (version 0.11.5)