##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779831_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	51566
Sequences flagged as poor quality	0
Sequence length	76
%GC	50
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.33194352868169	23.0	12.0	32.0	12.0	34.0
2	22.880890509250282	23.0	12.0	33.0	12.0	34.0
3	22.447058139083893	23.0	11.0	34.0	11.0	34.0
4	22.716615599425978	23.0	12.0	34.0	12.0	34.0
5	22.154694954039485	23.0	11.0	34.0	11.0	34.0
6	24.911472675794126	26.0	12.0	36.0	11.0	38.0
7	25.131501376876237	26.0	12.0	37.0	11.0	38.0
8	24.523271147655432	26.0	11.0	37.0	11.0	38.0
9	25.376333242834427	27.0	12.0	37.0	11.0	38.0
10-11	24.391391614629796	26.0	11.5	36.0	11.0	38.0
12-13	24.514272970561997	26.0	11.0	36.5	11.0	38.0
14-15	23.81500407245084	25.5	11.0	34.5	11.0	38.0
16-17	23.956550828065005	24.5	11.0	35.5	11.0	38.0
18-19	23.828918279486484	23.5	11.0	36.5	11.0	38.0
20-21	23.86226389481441	24.5	11.0	35.0	10.5	38.0
22-23	24.039211883799403	24.5	11.0	36.5	10.5	38.0
24-25	24.227000349067215	25.0	11.0	36.5	10.0	38.0
26-27	23.513506961951673	23.0	11.0	35.5	10.0	38.0
28-29	23.612079664895475	23.0	11.0	36.5	10.5	38.0
30-31	23.777886591940426	24.0	11.0	37.0	11.0	38.0
32-33	24.002734359849512	25.0	11.0	37.0	11.0	38.0
34-35	24.111798471861306	26.0	11.0	37.0	11.0	38.0
36-37	23.861090641120118	24.5	11.0	36.5	11.0	38.0
38-39	23.64763603925067	23.5	11.0	36.5	11.0	38.0
40-41	23.631336539580346	23.5	11.0	36.0	11.0	38.0
42-43	23.82702749873948	24.0	11.0	36.5	11.0	38.0
44-45	23.996771128262807	25.0	11.0	37.0	11.0	38.0
46-47	23.876556257999457	24.5	11.0	36.5	11.0	38.0
48-49	23.502472559438388	23.5	11.0	36.0	11.0	38.0
50-51	23.244356746693555	23.0	11.0	35.0	11.0	38.0
52-53	23.6790520885855	23.5	11.0	36.0	11.0	38.0
54-55	23.629930574409496	23.0	11.0	36.0	11.0	38.0
56-57	23.622832874374588	23.5	11.0	36.0	11.0	38.0
58-59	23.53240507311019	23.0	11.0	37.0	11.0	38.0
60-61	23.768170887794284	24.0	11.0	36.5	11.0	38.0
62-63	23.462455881782567	23.0	11.0	36.0	11.0	38.0
64-65	23.45560058953574	23.5	11.0	36.5	11.0	38.0
66-67	23.801594073614396	24.0	11.0	36.0	11.0	38.0
68-69	23.53353954155839	23.5	11.0	36.0	11.0	38.0
70-71	23.78162936818834	24.0	11.0	36.0	11.0	38.0
72-73	23.74772136679207	24.0	11.0	36.0	11.0	38.0
74-75	23.343365783655898	23.0	11.0	35.5	11.0	38.0
76	22.969049373618276	23.0	11.0	34.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	65.0
13	555.0
14	1935.0
15	3380.0
16	4475.0
17	5112.0
18	5204.0
19	4517.0
20	3419.0
21	2389.0
22	1543.0
23	1106.0
24	833.0
25	628.0
26	565.0
27	477.0
28	511.0
29	450.0
30	559.0
31	605.0
32	749.0
33	970.0
34	1296.0
35	1802.0
36	3046.0
37	5373.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.26384175312712	20.486764278095606	9.615048967322796	46.63434500145448
2	11.959430632587363	21.622774696505452	27.933134235736727	38.48466043517046
3	19.679633867276888	20.428189116859947	32.874374587906765	27.017802427956404
4	20.164449443431717	31.687546057479736	19.080401815149518	29.067602683939032
5	11.274871039056743	27.12252259240585	26.040414226428265	35.56219214210914
6	17.697707791955942	46.257223752084705	11.044098824807042	25.00096963115231
7	15.843773028739868	37.72253034945507	12.242562929061783	34.19113369274328
8	14.56082850105696	43.244186723037835	16.754261776855497	25.440722999049708
9	19.738551950192985	21.590798890591362	13.299326984619562	45.371322174596095
10-11	15.57981905805463	33.65655938793915	20.84614116574709	29.917480388259136
12-13	15.469495403948338	24.131210487530545	24.718806965830197	35.68048714269092
14-15	26.36911918706124	28.06985222821239	17.808245743319244	27.752782841407132
16-17	28.972578831012687	24.273746266920064	16.31598339991467	30.437691502152582
18-19	20.17143244999079	23.777525671233672	21.585168378082244	34.46587350069329
20-21	26.043323119885198	23.497071713920025	18.338633983632626	32.12097118256215
22-23	28.978958595946864	22.62969068166392	16.723552797440124	31.66779792494909
24-25	19.6365716723715	31.930882680914195	15.308012295280667	33.12453335143364
26-27	24.475429546600473	32.178179420548425	17.709343365783656	25.63704766706745
28-29	20.682098077053617	29.65002957633118	21.29592816345529	28.371944183159915
30-31	22.205523019043554	26.637707016251017	21.969902649032306	29.18686731567312
32-33	17.675577663360194	30.262481698034538	22.58486778951043	29.477072849094842
34-35	16.519926306603317	34.95878987685445	20.383011732764473	28.138272083777753
36-37	23.860649616417895	25.4483206765787	21.392339996314508	29.298689710688897
38-39	25.190795279332047	22.34559401091921	25.08218500596387	27.381425703784874
40-41	19.45311742460972	29.449238824784253	22.15941045282653	28.938233297779504
42-43	14.614564142344614	28.216813730243384	24.43711820032968	32.73150392708232
44-45	14.326025192238696	23.69214657655124	29.17761594926644	32.80421228194362
46-47	17.578784058954717	27.39552021720159	24.69019683894114	30.335498884902552
48-49	18.891690099873944	28.43207602055658	22.83040822263163	29.845825656937848
50-51	25.80773407803894	27.81882708866651	17.748817004111395	28.62462182918315
52-53	13.16026803463892	31.605589550140127	22.104130099591742	33.130012315629216
54-55	16.07160172992262	26.44628900568237	21.920757132052053	35.56135213234296
56-57	18.919102387556002	28.72825306929925	19.256579840570996	33.09606470257375
58-59	17.19245502594191	29.279930175047276	20.277360228870677	33.250254570140136
60-61	13.940698926295575	26.34795006837955	21.96098970912018	37.750361296204694
62-63	17.158013149983514	27.692546403149787	20.708314746213077	34.44112570065362
64-65	17.853921578135758	26.14999660553395	19.95461113966773	36.04147067666256
66-67	14.787011173184359	34.859947237740535	16.07988050900062	34.27316108007449
68-69	16.064190827111414	37.524483661398236	14.698923688548435	31.712401822941917
70-71	13.810724328517404	34.75710268593038	16.104916125278773	35.327256860273444
72-73	13.231900079517464	31.711952832567253	16.1304086421908	38.925738445724484
74-75	13.619612863183212	30.32894992047791	20.458512742930292	35.59292447340859
76	11.905362164258703	34.253854358576554	17.5254533113546	36.315330165810146
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	1.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.5
15	4.0
16	9.0
17	13.0
18	13.5
19	17.0
20	24.0
21	28.0
22	29.0
23	47.0
24	78.0
25	92.0
26	107.5
27	143.0
28	200.5
29	238.0
30	256.0
31	317.0
32	421.0
33	482.0
34	508.5
35	595.0
36	772.0
37	889.0
38	1005.0
39	1221.5
40	1392.5
41	1615.0
42	1767.0
43	1889.5
44	2218.5
45	2463.5
46	2502.0
47	2673.5
48	2891.5
49	2931.5
50	2925.0
51	2981.5
52	2949.5
53	2821.5
54	2782.0
55	2661.5
56	2460.0
57	2231.0
58	2083.0
59	1965.5
60	1685.5
61	1435.5
62	1348.0
63	1264.0
64	1056.0
65	872.5
66	734.0
67	655.0
68	566.0
69	441.0
70	362.0
71	319.0
72	300.5
73	224.5
74	159.5
75	152.0
76	122.0
77	98.5
78	84.0
79	63.0
80	54.5
81	46.0
82	33.0
83	20.0
84	21.0
85	18.0
86	13.5
87	13.0
88	13.0
89	11.0
90	7.5
91	5.5
92	5.0
93	3.5
94	2.5
95	1.5
96	0.0
97	1.0
98	4.0
99	5.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0019392623046193228
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.005817786913857969
9	0.01357483613233526
10-11	0.004848155761548307
12-13	0.0
14-15	0.0
16-17	0.0
18-19	9.696311523096614E-4
20-21	0.0
22-23	0.0019392623046193228
24-25	0.0029088934569289843
26-27	0.0
28-29	0.008726680370786954
30-31	0.0
32-33	9.696311523096614E-4
34-35	0.0019392623046193228
36-37	0.024240778807741534
38-39	0.010665942675406275
40-41	0.0019392623046193228
42-43	0.0019392623046193228
44-45	0.004848155761548307
46-47	0.0019392623046193228
48-49	0.0019392623046193228
50-51	0.0038785246092386456
52-53	0.010665942675406275
54-55	0.005817786913857969
56-57	0.01357483613233526
58-59	0.016483729589264244
60-61	0.0300585657215995
62-63	0.01357483613233526
64-65	0.022301516503122214
66-67	0.02714967226467052
68-69	0.0019392623046193228
70-71	0.0019392623046193228
72-73	0.009696311523096614
74-75	0.015514098436954582
76	0.0019392623046193228
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	51566.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.25714618159252
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.59627863005457	85.37990148547493
2	2.5761677624511194	4.650351006477136
3	1.4180739976795154	3.839739363146259
4	0.7820892956899145	2.823565915525734
5	0.3502213054875167	1.5804987782647482
6	0.1589961754974002	0.8610324632509794
7	0.07090369988397577	0.4479695923670635
8	0.03008035752653517	0.21719737811736417
9	0.01074298483090542	0.08726680370786953
>10	0.006445790898543251	0.11247721366792073
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.013574836132335259	0.0
2	0.0	0.0	0.0	0.013574836132335259	0.0
3	0.0	0.0	0.0	0.013574836132335259	0.0
4	0.0	0.0	0.0	0.017453360741573904	0.0
5	0.0	0.0	0.0	0.027149672264670518	0.0
6	0.0	0.0	0.0	0.027149672264670518	0.0
7	0.0	0.0	0.0	0.027149672264670518	0.0
8	0.0	0.0	0.0	0.02908893456928984	0.0
9	0.0	0.0	0.0	0.02908893456928984	0.0
10	0.0	0.0	0.0	0.036845983787767135	0.0
11	0.0	0.0	0.0	0.036845983787767135	0.0
12	0.0	0.0	0.0	0.0426637707016251	0.0
13	0.0	0.0	0.0	0.0426637707016251	0.0
14	0.0	0.0	0.0	0.0426637707016251	0.0
15	0.0	0.0	0.0	0.0426637707016251	0.0
16	0.0	0.0	0.0	0.0426637707016251	0.0
17	0.0	0.0	0.0	0.0426637707016251	0.0
18	0.0	0.0	0.0	0.04654229531086375	0.0
19	0.0	0.0	0.0	0.04848155761548307	0.0
20	0.0	0.0	0.0	0.05817786913857968	0.0
21	0.0	0.0	0.0	0.06205639374781833	0.0
22	0.0	0.0	0.0	0.07757049218477291	0.0
23	0.0	0.0	0.0	0.08144901679401156	0.0
24	0.0	0.0	0.0	0.10859868905868207	0.0
25	0.0	0.0	0.0	0.10859868905868207	0.0
26	0.0	0.0	0.0	0.10859868905868207	0.0
27	0.0	0.0	0.0	0.13186983671411395	0.0
28	0.0	0.0	0.0	0.13962688593259123	0.0
29	0.0	0.0	0.0	0.16483729589264243	0.0
30	0.0	0.0	0.0	0.22495442733584145	0.0
31	0.0	0.0	0.0	0.23271147655431873	0.0
32	0.0	0.0	0.0	0.246286312686654	0.0
33	0.0	0.0	0.0	0.306403444129853	0.0
34	0.0	0.0	0.0	0.35488500174533605	0.0
35	0.0	0.0	0.0	0.4169413954931544	0.0
36	0.0	0.0	0.0	0.5139045107241206	0.0
37	0.0	0.0	0.0	0.7097700034906721	0.0
38	0.0	0.0	0.0	0.9541170538727068	0.0
39	0.0	0.0	0.0	1.1461040220300198	0.0
40	0.0	0.0	0.0	1.4253577938952022	0.0
41	0.0	0.0	0.0	1.7918783694682543	0.0
42	0.0	0.0	0.0	2.1758523057828802	0.0
43	0.0	0.0	0.0	2.5656440290113642	0.0
44	0.0	0.0	0.0	2.9767676375906604	0.0
45	0.0	0.0	0.0	3.4092231315207693	0.0
46	0.0	0.0	0.0	3.7990148547492533	0.0
47	0.0	0.0	0.0	4.217895512547027	0.0
48	0.0	0.0	0.0	4.518481169763022	0.0
49	0.0	0.0	0.0	4.890819532249932	0.0
50	0.0	0.0	0.0	5.236008222472171	0.0
51	0.0	0.0	0.0	5.538533141992786	0.0
52	0.0	0.0	0.0	5.889539619128883	0.0
53	0.0	0.0	0.0	6.240546096264981	0.0
54	0.0	0.0	0.0	6.634216344102703	0.0
55	0.0	0.0	0.0	6.961951673583369	0.0
56	0.0	0.0	0.0	7.223752084706978	0.0
57	0.0	0.0	0.0	7.537912578055308	0.0
58	0.0	0.0	0.0	7.84431602218516	0.0
59	0.0	0.0	0.0	8.034363728037855	0.0
60	0.0	0.0	0.0	8.269014466896792	0.0
61	0.0	0.0	0.0	8.499786681146492	0.0
62	0.0	0.0	0.0	8.724741108482334	0.0
63	0.0	0.0	0.0	8.928363650467363	0.0
64	0.0	0.0	0.0	9.153318077803204	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGATTC	15	0.002212836	70.00001	34
TTGTGTA	15	0.002212836	70.00001	34
CAAGTGT	15	0.002212836	70.00001	33
GATCATT	15	0.002212836	70.00001	58
TTCGGTG	15	0.002212836	70.00001	44
CAGTACG	15	0.002212836	70.00001	53
AGTGTCG	15	0.002212836	70.00001	35
TTGTCAT	20	7.857113E-5	70.0	2
AGATTTC	20	7.857113E-5	70.0	40
CTCGGTG	60	1.8189894E-12	58.333336	44
TGCCTAC	30	8.267576E-6	58.333336	19
TGGTCGC	60	1.8189894E-12	58.333336	49
ACGTGTA	55	3.45608E-11	57.27273	34
GGTCGCC	75	0.0	56.0	50
TTGATGC	25	2.3696036E-4	55.999996	12
TGTCATC	25	2.3696036E-4	55.999996	3
TCGGCGG	50	9.513315E-10	55.999996	45
AATGATT	25	2.3696036E-4	55.999996	33
TCTCGGT	50	9.513315E-10	55.999996	43
GTATATC	25	2.3696036E-4	55.999996	30
>>END_MODULE