FastQCFastQC Report
Wed 25 May 2016
SRR1779831_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779831_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51566
Sequences flagged as poor quality0
Sequence length76
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT2342345.4233409610984No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG29785.7751231431563435No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC17163.327774114726758No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT6481.2566419733933212RNA PCR Primer, Index 30 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC5951.1538610712484971No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT5090.9870845130512352No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGT4930.9560563161773261No Hit
CTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC2580.5003296745917852No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAGCTCGTATGCCGT2160.4188806577977737No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATATCGTATGCCGT1930.3742776247915293No Hit
CTTTACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC1560.30252491952061433No Hit
CTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG1520.2947678703021371No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCG1240.240468525772796No Hit
CCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG1150.22301516503122212No Hit
CTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG910.1764728697203584No Hit
TTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC850.16483729589264243No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC740.1435054105418299No Hit
TATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCT730.14156614823721056RNA PCR Primer, Index 30 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCC730.14156614823721056No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCG630.12217352519101733No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGCTCTCGTATGCCGT580.11247721366792073No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCCGGATCTCGTATGCCGT550.10665942675406276No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTCTGCCGT540.10472016444944343No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGCC150.00221453969.986438
ACCGGAT150.00221453969.9864332
TTACACA150.00221453969.986433
CTGGTCT150.00221453969.9864319
CACCTCT150.00221453969.986437
ACCCACC150.00221453969.9864370
GAGACAC150.00221453969.9864324
TAGGTTT150.00221453969.9864324
AAACTAA207.864666E-569.9864270
CTTGCCG252.805893E-669.9864229
CGAGACC252.805893E-669.9864223
TCTTTAT750.065.384071
GAAATTG352.92408E-759.9883626
AAATTGC352.92408E-759.9883627
ACGAGAA352.92408E-759.9883622
CGAGAAA352.92408E-759.9883623
GCTTGAC308.229505E-658.37864356
TCTTAAT308.229505E-658.3786431
CCTTGCC308.277089E-658.3220228
CCTTATA252.3605088E-456.0434951
Produced by FastQC (version 0.11.5)