##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779831_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	51566
Sequences flagged as poor quality	0
Sequence length	76
%GC	45
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.549431796144745	34.0	34.0	34.0	34.0	34.0
2	33.68349299926308	34.0	34.0	34.0	34.0	34.0
3	33.78825194895862	34.0	34.0	34.0	34.0	34.0
4	33.600880425086295	34.0	34.0	34.0	34.0	34.0
5	33.6495365163092	34.0	34.0	34.0	34.0	34.0
6	37.367335065740996	38.0	38.0	38.0	37.0	38.0
7	37.40761354380793	38.0	38.0	38.0	37.0	38.0
8	37.32112244502191	38.0	38.0	38.0	37.0	38.0
9	37.261781018500564	38.0	38.0	38.0	37.0	38.0
10-11	37.18261063491448	38.0	38.0	38.0	36.5	38.0
12-13	37.34038901601831	38.0	38.0	38.0	36.5	38.0
14-15	37.34555715006012	38.0	38.0	38.0	37.0	38.0
16-17	37.119865803048526	38.0	38.0	38.0	36.0	38.0
18-19	36.923777295116935	38.0	38.0	38.0	34.5	38.0
20-21	37.06374355195284	38.0	38.0	38.0	35.5	38.0
22-23	37.132471008028546	38.0	38.0	38.0	36.0	38.0
24-25	37.226447659310395	38.0	38.0	38.0	36.0	38.0
26-27	36.96795369041617	38.0	38.0	38.0	35.5	38.0
28-29	36.41201566923942	38.0	37.0	38.0	34.0	38.0
30-31	36.99238839545437	38.0	37.0	38.0	36.0	38.0
32-33	36.49130240856378	38.0	37.0	38.0	34.0	38.0
34-35	36.54824884613893	38.0	37.0	38.0	34.0	38.0
36-37	36.31664274909824	38.0	37.0	38.0	34.0	38.0
38-39	32.481664274909825	36.5	31.0	38.0	17.0	38.0
40-41	35.47878447038747	37.5	35.5	38.0	30.5	38.0
42-43	36.90606213396424	38.0	37.0	38.0	35.0	38.0
44-45	36.667261373773414	38.0	37.0	38.0	34.5	38.0
46-47	36.853236628786405	38.0	37.5	38.0	35.5	38.0
48-49	36.41698987705077	38.0	37.0	38.0	34.0	38.0
50-51	36.38584532443858	38.0	37.0	38.0	33.5	38.0
52-53	36.31239576465113	38.0	37.0	38.0	34.0	38.0
54-55	36.345140208664624	38.0	37.0	38.0	34.0	38.0
56-57	36.17996354186867	38.0	37.0	38.0	33.0	38.0
58-59	36.14868324089517	38.0	37.0	38.0	33.5	38.0
60-61	35.600279253771866	38.0	36.5	38.0	31.0	38.0
62-63	35.63984990109762	38.0	37.0	38.0	31.5	38.0
64-65	36.31866927820657	38.0	37.0	38.0	34.0	38.0
66-67	35.83657836558973	38.0	36.5	38.0	32.5	38.0
68-69	35.53833921576232	38.0	36.5	38.0	30.0	38.0
70-71	31.90174727533646	35.5	29.0	38.0	17.5	38.0
72-73	25.054551448628942	24.0	15.0	37.5	10.0	38.0
74-75	23.959566380948687	23.0	11.0	37.0	10.5	38.0
76	23.95064577434744	24.0	11.0	37.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	7.0
24	17.0
25	54.0
26	87.0
27	206.0
28	366.0
29	588.0
30	988.0
31	1417.0
32	2129.0
33	3501.0
34	6367.0
35	14598.0
36	10405.0
37	10836.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.541727302714554	7.955449482895784	12.845140384578071	65.65768282981159
2	6.4402901136407715	6.946437575146415	69.82507854012334	16.788193771089478
3	6.888259706007835	8.872125043633401	70.96924329984874	13.270371950510027
4	5.04014272970562	57.38858938059962	25.536206027227244	12.035061862467519
5	4.7473141217081025	21.0157855951596	68.78951246945661	5.447387813675678
6	10.148159640072917	68.52577279602839	13.888996625683589	7.437070938215102
7	9.178528487763254	19.72423690028313	12.545087848582398	58.55214676337121
8	7.491370282744445	68.78951246945661	8.144901679401157	15.574215568397781
9	7.910250940542218	14.499864251638678	7.041461428072761	70.54842337974635
10-11	8.44548733661715	40.68378388860877	35.361478493581046	15.509250281193035
12-13	8.805220494124036	10.185005623860684	41.472094015436525	39.53767986657875
14-15	9.125198774386224	8.49978668114649	14.26521351277974	68.10980103168754
16-17	38.171469572974445	35.172400418880656	7.697901718186402	18.9582282899585
18-19	40.594577822596285	15.824380405693676	8.19338323701664	35.3876585346934
20-21	11.403831982313928	8.921576232401195	8.124539425202652	71.55005236008223
22-23	9.294884226040415	38.78039793662491	7.947096924329985	43.97762091300469
24-25	38.2471008028546	38.56126129620293	7.634875693286274	15.556762207656208
26-27	40.52476437962999	42.23907225691347	8.752860411899313	8.483302951557228
28-29	11.116821161230268	41.18217430089594	10.05022689368964	37.65077764418415
30-31	8.352402745995423	14.926501958654928	64.4921072024202	12.228988092929448
32-33	38.684404452546254	8.44936586122639	17.797579800643835	35.068649885583525
34-35	37.46072993833146	9.04956754450607	9.123259512081603	44.36644300508087
36-37	43.96210681456773	34.67304037544118	9.693402629639685	11.671450180351394
38-39	11.5114610402203	15.92328278322926	36.84501415661482	35.72024201993562
40-41	8.148780204010395	9.058294224876857	41.53415040918434	41.258775161928405
42-43	37.746167495709344	8.231278664998886	38.82925599480272	15.193297844489049
44-45	10.82302292208044	37.14075165806927	42.14404840398712	9.892177015863165
46-47	37.58193383237017	10.588372183221502	16.769770779195596	35.05992320521274
48-49	36.18372571073964	7.6057867587169845	10.747391692200287	45.463095838343094
50-51	13.43367534961305	7.932967395310045	40.243807824349744	38.38954943072716
52-53	7.2380545909558265	7.903466739092478	69.18928356645392	15.66919510349778
54-55	7.214374417882645	8.045832039739212	46.03675100900341	38.70304253337473
56-57	36.77730452415372	7.794778255343508	17.552319320067138	37.87559790043563
58-59	9.884279243013589	9.545749953925096	64.71050410794138	15.85946669511994
60-61	36.0431054551098	36.220609916970595	18.24513075192054	9.491153875999068
62-63	9.291069748494166	72.78344115849814	8.394843790919408	9.530645302088283
64-65	6.239756383773142	76.09515773956727	8.287024914414284	9.378060962245304
66-67	6.557313441522538	76.03670543500402	7.644702253348982	9.761278870124453
68-69	6.763833129691045	75.80535676189368	7.754892263532515	9.67591784488276
70-71	7.345125877652353	71.00643934985841	8.21695954071143	13.431475231777803
72-73	9.39060939060939	57.693762548131474	10.903659447348769	22.011968613910362
74-75	10.987199379363847	51.37994569433669	12.667765709852599	24.96508921644686
76	10.828791434722735	52.70671295847315	11.963457920359991	24.50103768644413
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	2.5
16	7.5
17	10.0
18	12.0
19	15.0
20	28.5
21	41.0
22	41.5
23	54.5
24	68.5
25	70.0
26	92.5
27	141.5
28	176.0
29	184.0
30	201.0
31	233.5
32	308.5
33	368.0
34	363.5
35	412.5
36	511.0
37	556.0
38	599.5
39	670.5
40	794.5
41	4393.5
42	7896.0
43	9671.5
44	10379.5
45	7385.5
46	5459.0
47	4128.0
48	2188.0
49	1237.5
50	896.0
51	788.5
52	661.5
53	629.5
54	617.0
55	560.0
56	518.0
57	540.5
58	548.0
59	525.5
60	480.5
61	425.5
62	393.0
63	358.5
64	345.0
65	313.0
66	231.5
67	203.0
68	201.0
69	166.5
70	150.0
71	166.0
72	131.0
73	87.0
74	83.5
75	89.0
76	65.5
77	47.0
78	38.0
79	24.0
80	26.0
81	24.5
82	21.5
83	22.0
84	14.0
85	7.0
86	4.5
87	1.0
88	1.5
89	1.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05623860683396036
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	9.696311523096614E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.017453360741573908
52-53	0.036845983787767135
54-55	0.05817786913857968
56-57	0.061086762595508666
58-59	0.03781561494007679
60-61	0.034906721483147815
62-63	0.031997828026218825
64-65	0.018422991893883568
66-67	0.03975487724469612
68-69	0.009696311523096614
70-71	0.015514098436954582
72-73	0.02811930341698018
74-75	0.011635573827715937
76	0.017453360741573908
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	51566.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.190900981266726
#Duplication Level	Percentage of deduplicated	Percentage of total
1	54.82093663911846	8.876003568242641
2	14.600550964187327	4.727921498661909
3	10.659959276560068	5.177830353333592
4	7.5098814229249005	4.8636698599852615
5	5.270092226613966	4.26637707016251
6	3.0183255479698166	2.932164604584416
7	1.5570727033177627	1.7647286972035838
8	0.7545813869924541	0.9773882015281387
9	0.3832794346628339	0.558507543730365
>10	1.137860821655288	3.308381491680565
>50	0.1197748233321356	1.3128805802272816
>100	0.09581985866570847	3.310320753985184
>500	0.035932446999640676	3.397587557693053
>1k	0.023954964666427117	9.1028972578831
>5k	0.0	0.0
>10k+	0.011977482333213559	45.4233409610984
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT	23423	45.4233409610984	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG	2978	5.7751231431563435	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC	1716	3.327774114726758	No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT	648	1.2566419733933212	RNA PCR Primer, Index 30 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC	595	1.1538610712484971	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT	509	0.9870845130512352	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGT	493	0.9560563161773261	No Hit
CTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC	258	0.5003296745917852	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAGCTCGTATGCCGT	216	0.4188806577977737	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATATCGTATGCCGT	193	0.3742776247915293	No Hit
CTTTACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC	156	0.30252491952061433	No Hit
CTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG	152	0.2947678703021371	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCG	124	0.240468525772796	No Hit
CCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG	115	0.22301516503122212	No Hit
CTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG	91	0.1764728697203584	No Hit
TTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC	85	0.16483729589264243	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC	74	0.1435054105418299	No Hit
TATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCT	73	0.14156614823721056	RNA PCR Primer, Index 30 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCC	73	0.14156614823721056	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCG	63	0.12217352519101733	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGCTCTCGTATGCCGT	58	0.11247721366792073	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCCGGATCTCGTATGCCGT	55	0.10665942675406276	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTCTGCCGT	54	0.10472016444944343	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.023271147655431874	0.0
2	0.0	0.0	0.0	0.023271147655431874	0.0
3	0.0	0.0	0.0	0.023271147655431874	0.0
4	0.0	0.0	0.0	0.02908893456928984	0.0
5	0.0	0.0	0.0	0.038785246092386455	0.0
6	0.0	0.0	0.0	0.038785246092386455	0.0
7	0.0	0.0	0.0	0.038785246092386455	0.0
8	0.0	0.0	0.0	0.038785246092386455	0.0
9	0.0	0.0	0.0	0.038785246092386455	0.0
10	0.0	0.0	0.0	0.04654229531086375	0.0
11	0.0	0.0	0.0	0.04654229531086375	0.0
12	0.0	0.0	0.0	0.05817786913857968	0.0
13	0.0	0.0	0.0	0.05817786913857968	0.0
14	0.0	0.0	0.0	0.05817786913857968	0.0
15	0.0	0.0	0.0	0.05817786913857968	0.0
16	0.0	0.0	0.0	0.05817786913857968	0.0
17	0.0	0.0	0.0	0.05817786913857968	0.0
18	0.0	0.0	0.0	0.06205639374781833	0.0
19	0.0	0.0	0.0	0.06205639374781833	0.0
20	0.0	0.0	0.0	0.08144901679401156	0.0
21	0.0	0.0	0.0	0.08726680370786953	0.0
22	0.0	0.0	0.0	0.08726680370786953	0.0
23	0.0	0.0	0.0	0.08920606601248884	0.0
24	0.0	0.0	0.0	0.10859868905868207	0.0
25	0.0	0.0	0.0	0.1105379513633014	0.0
26	0.0	0.0	0.0	0.1105379513633014	0.0
27	0.0	0.0	0.0	0.13962688593259123	0.0
28	0.0	0.0	0.0	0.15901950897878447	0.0
29	0.0	0.0	0.0	0.18422991893883567	0.0
30	0.0	0.0	0.0	0.246286312686654	0.0
31	0.0	0.0	0.0	0.2559826242097506	0.0
32	0.0	0.0	0.0	0.2695574603420859	0.0
33	0.0	0.0	0.0	0.33549237869914283	0.0
34	0.0	0.0	0.0	0.39367024783772253	0.0
35	0.0	0.0	0.0	0.4615444284993988	0.0
36	0.0	0.0	0.0	0.564325330644223	0.0
37	0.0	0.0	0.0	0.7854012333708258	0.0
38	0.0	0.0	0.0	1.0278090214482412	0.0
39	0.0	0.0	0.0	1.2333708257378893	0.0
40	0.0	0.0	0.0	1.5145638599076912	0.0
41	0.0	0.0	0.0	1.8888414846992203	0.0
42	0.0	0.0	0.0	2.284450994841562	0.0
43	0.0	0.0	0.0	2.6819997672885236	0.0
44	0.0	0.0	0.0	3.110576736609394	0.0
45	0.0	0.0	0.0	3.556607066671838	0.0
46	0.0	0.0	0.0	3.938641740681845	0.0
47	0.0	0.0	0.0	4.363340185393477	0.0
48	0.0	0.0	0.0	4.671682891827949	0.0
49	0.0	0.0	0.0	5.073110188884148	0.0
50	0.0	0.0	0.0	5.433812977543343	0.0
51	0.0	0.0	0.0	5.72664158554086	0.0
52	0.0	0.0	0.0	6.0718302757631	0.0
53	0.0	0.0	0.0	6.409261916766862	0.0
54	0.0	0.0	0.0	6.789357328472249	0.0
55	0.0	0.0	0.0	7.109335608734438	0.0
56	0.0	0.0	0.0	7.373075282162666	0.0
57	0.0	0.0	0.0	7.70274987394795	0.0
58	0.0	0.0	0.0	8.0188496296009	0.0
59	0.0	0.0	0.0	8.212775860062832	0.0
60	0.0	0.0	0.0	8.451305123531009	0.0
61	0.0	0.0	0.0	8.67819881317147	0.0
62	0.0	0.0	0.0	8.90703176511655	0.0
63	0.0	0.0	0.0	9.118411356320056	0.0
64	0.0	0.0	0.0	9.358879882092852	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTTGCC	15	0.002214539	69.98643	8
ACCGGAT	15	0.002214539	69.98643	32
TTACACA	15	0.002214539	69.98643	3
CTGGTCT	15	0.002214539	69.98643	19
CACCTCT	15	0.002214539	69.98643	7
ACCCACC	15	0.002214539	69.98643	70
GAGACAC	15	0.002214539	69.98643	24
TAGGTTT	15	0.002214539	69.98643	24
AAACTAA	20	7.864666E-5	69.98642	70
CTTGCCG	25	2.805893E-6	69.98642	29
CGAGACC	25	2.805893E-6	69.98642	23
TCTTTAT	75	0.0	65.38407	1
GAAATTG	35	2.92408E-7	59.98836	26
AAATTGC	35	2.92408E-7	59.98836	27
ACGAGAA	35	2.92408E-7	59.98836	22
CGAGAAA	35	2.92408E-7	59.98836	23
GCTTGAC	30	8.229505E-6	58.378643	56
TCTTAAT	30	8.229505E-6	58.378643	1
CCTTGCC	30	8.277089E-6	58.32202	28
CCTTATA	25	2.3605088E-4	56.043495	1
>>END_MODULE