FastQCFastQC Report
Wed 25 May 2016
SRR1779830_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779830_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248748
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCT453.8380676E-1062.2163213
CGTGCGA252.3885016E-455.9946910
GCGTCAT200.006937223552.51614457
ATTCGTT200.006942744452.5055868
GCCGTAT1000.052.50557754
CTCGTAT200.00694826852.49502648
CCGTGCG451.6815266E-646.662249
TGGTCGC1250.044.79575349
ACGATAT551.3059798E-744.5501924
TCTTACG551.3080353E-744.54123311
CGCCGTA1600.043.75465453
GTCCGTG404.5869412E-543.7458577
CGGTGGT1650.042.42022346
ATCTCGG1900.042.36440342
TCGGTGG1600.041.55856345
TACGCTG602.5907866E-740.82946414
CGACGAT1300.040.38078722
TCTCGGT1650.040.2992143
GCGAGAT350.001254456839.9962138
CATCTCG350.001254456839.9962141
Produced by FastQC (version 0.11.5)