##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1779830_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 248748 Sequences flagged as poor quality 0 Sequence length 76 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.894576036792255 34.0 23.0 34.0 12.0 34.0 2 29.5442094006786 34.0 27.0 34.0 12.0 34.0 3 29.214779616318523 34.0 27.0 34.0 11.0 34.0 4 29.517234309421582 34.0 27.0 34.0 12.0 34.0 5 30.075546336050945 34.0 32.0 34.0 11.0 34.0 6 32.64909868622059 37.0 31.0 38.0 12.0 38.0 7 32.52917008377957 37.0 31.0 38.0 12.0 38.0 8 32.03150176081818 37.0 31.0 38.0 11.0 38.0 9 32.93741859231029 37.0 34.0 38.0 12.0 38.0 10-11 31.536538987248136 37.0 28.5 38.0 11.5 38.0 12-13 32.36929744158747 37.0 31.0 38.0 11.0 38.0 14-15 31.171862688343225 37.0 27.0 38.0 11.0 38.0 16-17 31.79816119124576 37.0 28.5 38.0 11.0 38.0 18-19 32.65782237445125 37.5 32.0 38.0 11.0 38.0 20-21 31.369810008522684 37.0 27.5 38.0 11.0 38.0 22-23 32.25622718574621 37.0 30.0 38.0 11.0 38.0 24-25 32.56838446942287 37.5 32.0 38.0 11.0 38.0 26-27 31.472809831636837 37.0 26.5 38.0 11.0 38.0 28-29 32.67579437824625 37.5 32.5 38.0 11.0 38.0 30-31 33.035962098187724 38.0 34.0 38.0 11.0 38.0 32-33 33.144965989676294 38.0 34.0 38.0 11.0 38.0 34-35 33.07389808159262 38.0 34.0 38.0 11.0 38.0 36-37 32.58606300352163 38.0 32.5 38.0 11.0 38.0 38-39 32.52889872481387 37.5 32.5 38.0 11.0 38.0 40-41 32.5325751362825 37.5 32.0 38.0 11.0 38.0 42-43 32.89607956646888 38.0 33.0 38.0 11.0 38.0 44-45 32.97995561773361 38.0 34.0 38.0 11.0 38.0 46-47 32.894240355701356 38.0 34.0 38.0 11.0 38.0 48-49 32.57087494170807 37.5 32.5 38.0 11.0 38.0 50-51 31.96192532201264 37.5 29.5 38.0 11.0 38.0 52-53 32.52221927412482 37.0 31.5 38.0 11.0 38.0 54-55 32.66037917892807 37.5 32.5 38.0 11.0 38.0 56-57 32.75391158923891 38.0 33.0 38.0 11.0 38.0 58-59 32.987796886809136 38.0 34.0 38.0 11.0 38.0 60-61 32.86937382411115 38.0 34.0 38.0 11.0 38.0 62-63 32.86689943235724 38.0 34.0 38.0 11.0 38.0 64-65 32.85622798977278 38.0 33.5 38.0 11.0 38.0 66-67 32.71343689195491 38.0 33.0 38.0 11.0 38.0 68-69 32.11977382732725 37.0 31.0 38.0 11.0 38.0 70-71 32.133420168202356 37.0 31.0 38.0 11.0 38.0 72-73 32.3219483171724 37.0 31.0 38.0 11.0 38.0 74-75 32.28938323122196 37.0 31.0 38.0 11.0 38.0 76 31.294547091835913 37.0 27.0 38.0 11.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 0.0 12 73.0 13 666.0 14 2228.0 15 3861.0 16 5163.0 17 6104.0 18 6511.0 19 5671.0 20 4681.0 21 3483.0 22 2756.0 23 2427.0 24 2239.0 25 2399.0 26 2727.0 27 3176.0 28 3737.0 29 4369.0 30 5614.0 31 6783.0 32 8490.0 33 10947.0 34 15420.0 35 22456.0 36 39686.0 37 77080.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.85860388827247 22.007815138212166 9.627414089761526 29.50616688375384 2 20.151719812822616 24.945326193577436 29.421744094424877 25.481209899175067 3 18.08416550082815 21.982086288130958 32.202871982890315 27.73087622815058 4 13.294177239615998 21.96560374354769 30.766880537732966 33.97333847910335 5 13.582420763182016 33.43946483991831 34.628218116326565 18.349896280573113 6 26.3531984691088 39.884379120702405 17.057295854372367 16.70512655581642 7 26.037034952439917 29.082407995561592 19.0903681785654 25.790188873433088 8 23.529104336604448 36.05407991380662 18.50624942209429 21.910566327494642 9 23.055120009649016 18.42761227033329 19.503075624170787 39.0141920958469 10-11 23.193710752506615 26.747191869487256 24.076151192802183 25.982946185203946 12-13 23.238578802643637 23.29727274189139 25.434375351761624 28.02977310370335 14-15 24.61416620099941 24.98060278113907 22.104186181139873 28.30104483672165 16-17 24.791355106372713 26.398001189959313 23.04098927428562 25.76965442938235 18-19 21.994990813811846 26.16254527765605 25.257193167244907 26.585270741287193 20-21 24.067932204480037 25.4701545339058 24.61366523549938 25.848248026114785 22-23 24.80833785603731 25.512874630645843 23.689119379284005 25.989668134032843 24-25 22.057706146504625 27.933248372818532 23.546190244549596 26.462855236127247 26-27 23.309828862257636 27.884155386798636 24.08089343791081 24.72512231303292 28-29 22.140073049777975 27.563325553954115 25.39434618036501 24.902255215902898 30-31 22.574945627484954 26.748664305499158 25.146835941740004 25.529554125275883 32-33 21.58654860852873 27.408315661463934 25.24005266384587 25.76508306616147 34-35 21.97980236550321 28.079295012502914 24.845824187310548 25.095078434683327 36-37 23.835218762753538 26.014503097036457 24.855099706279113 25.295178433930893 38-39 23.323315676794888 25.573167422178887 26.098441065020957 25.00507583600527 40-41 21.83769419871234 26.73806465618612 25.924585873570084 25.499655271531456 42-43 20.714663321895987 26.30026955153138 26.585901711179854 26.39916541539278 44-45 20.35194011309202 24.78094200465957 27.93228147972445 26.934836402523963 46-47 20.8134163648132 25.14965365581066 27.473757844504927 26.56317213487121 48-49 20.7875456034734 25.90176785696339 27.568920290656184 25.741766248907023 50-51 22.91512559096903 25.77469205287364 25.726248673334833 25.5839336828225 52-53 18.66972762927508 26.980763779859966 27.014335195508266 27.335173395356694 54-55 19.45479143088604 25.969472983811798 26.708224363316923 27.867511221985243 56-57 19.69021389514313 26.729856867159857 26.024847217754903 27.555082019942102 58-59 19.30985957964357 26.702785288530162 26.307155708786073 27.680199423040197 60-61 18.967045913761662 25.747855300378575 26.498374511700096 28.786724274159663 62-63 19.741079505477938 25.962006231782087 26.006834857774653 28.290079404965322 64-65 20.330685517027852 25.379027766887617 25.45623061132992 28.83405610475461 66-67 19.863411652831584 27.24871181949591 24.09576061454704 28.79211591312547 68-69 19.863757150840012 27.956719076008955 23.47023280682782 28.709290966323213 70-71 19.86476218808669 27.4365117971272 23.46018243436102 29.23854358042509 72-73 19.60604976340472 26.423491478951668 23.530877995633944 30.439580762009673 74-75 19.47259339073239 25.97069471889807 24.439293762425798 30.117418127943747 76 18.525293377448833 26.58829867211276 23.769302205908957 31.11710574452945 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 1.5 12 3.0 13 3.0 14 4.0 15 9.0 16 12.0 17 11.0 18 21.5 19 39.5 20 59.0 21 71.0 22 88.0 23 176.5 24 337.0 25 426.0 26 527.5 27 792.0 28 1047.0 29 1139.0 30 1333.0 31 1850.0 32 2550.5 33 2928.0 34 3504.5 35 4667.5 36 5839.0 37 6424.0 38 7329.5 39 9097.5 40 10797.0 41 12241.0 42 12848.0 43 13343.0 44 14429.0 45 15242.5 46 15465.0 47 15653.0 48 15481.5 49 14728.5 50 14335.0 51 13604.5 52 12248.0 53 10818.5 54 10015.0 55 9323.5 56 8105.0 57 6897.0 58 6216.0 59 5593.0 60 4644.5 61 3916.0 62 3513.0 63 3210.5 64 2728.0 65 2403.0 66 2130.5 67 2003.0 68 1849.0 69 1602.0 70 1384.0 71 1259.0 72 1186.0 73 1034.5 74 890.0 75 824.0 76 779.0 77 651.5 78 535.5 79 502.0 80 448.0 81 396.0 82 322.5 83 247.0 84 226.5 85 177.0 86 131.0 87 114.0 88 95.0 89 71.5 90 46.5 91 24.0 92 22.0 93 18.5 94 14.0 95 11.5 96 10.0 97 8.5 98 8.0 99 8.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0016080531300754178 7 0.004020132825188544 8 0.002010066412594272 9 0.00723623908533938 10-11 0.0024120796951131265 12-13 0.0 14-15 4.0201328251885445E-4 16-17 0.0 18-19 0.002010066412594272 20-21 0.0 22-23 0.0012060398475565632 24-25 0.002010066412594272 26-27 4.0201328251885445E-4 28-29 0.005427179314004535 30-31 4.0201328251885445E-4 32-33 2.0100664125942722E-4 34-35 0.0024120796951131265 36-37 0.01708556450705131 38-39 0.008241272291636516 40-41 6.030199237782816E-4 42-43 6.030199237782816E-4 44-45 0.0050251660314856806 46-47 0.0028140929776319813 48-49 2.0100664125942722E-4 50-51 0.0016080531300754178 52-53 0.009849325421711934 54-55 0.007035232444079952 56-57 0.011256371910527925 58-59 0.01226140511682506 60-61 0.021507710614758712 62-63 0.00924630549793365 64-65 0.021105697332239857 66-67 0.017889591072089022 68-69 4.0201328251885445E-4 70-71 4.0201328251885445E-4 72-73 0.004422146107707399 74-75 0.00864328557415537 76 0.0028140929776319813 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 248748.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.074211989416796 #Duplication Level Percentage of deduplicated Percentage of total 1 79.06319207453582 48.28722153320179 2 7.597918079267149 9.280737189027686 3 4.977600993118246 9.120091747573058 4 3.1189920051205977 7.619599156561262 5 1.8727919031924987 5.718964485382099 6 1.078569419656317 3.9523666428835287 7 0.6172404848382088 2.6388233353621415 8 0.3891345572154986 1.9012869151829759 9 0.2671838379778608 1.4686238124725277 >10 1.0126666676039684 9.840101224964124 >50 0.004709977473830496 0.17218395738882933 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.004824159390226253 0.0 2 0.0 0.0 0.0 0.004824159390226253 0.0 3 0.0 0.0 0.0 0.004824159390226253 0.0 4 0.0 0.0 0.0 0.005226172672745107 0.0 5 0.0 0.0 0.0 0.006432212520301671 0.0 6 0.0 0.0 0.0 0.006432212520301671 0.0 7 0.0 0.0 0.0 0.006432212520301671 0.0 8 0.0 0.0 0.0 0.006432212520301671 0.0 9 0.0 0.0 0.0 0.011658385193046778 0.0 10 0.0 0.0 0.0 0.013266438323122196 0.0 11 0.0 0.0 0.0 0.014874491453197613 0.0 12 0.0 0.0 0.0 0.016080531300754177 0.0 13 0.0 0.0 0.0 0.016482544583273032 0.0 14 0.0 0.0 0.0 0.016884557865791884 0.0 15 0.0 0.0 0.0 0.018492610995867305 0.0 16 0.0 0.0 0.0 0.018894624278386157 0.0 17 0.0 0.0 0.0 0.019296637560905012 0.0 18 0.0 0.0 0.0 0.020502677408461574 0.0 19 0.0 0.0 0.0 0.022512743821055847 0.0 20 0.0 0.0 0.0 0.02532683679868783 0.0 21 0.0 0.0 0.0 0.03015099618891408 0.0 22 0.0 0.0 0.0 0.043819447794555134 0.0 23 0.0 0.0 0.0 0.045829514207149404 0.0 24 0.0 0.0 0.0 0.05145770016241337 0.0 25 0.0 0.0 0.0 0.05226172672745107 0.0 26 0.0 0.0 0.0 0.05306575329248878 0.0 27 0.0 0.0 0.0 0.08402077604644058 0.0 28 0.0 0.0 0.0 0.1085435862800907 0.0 29 0.0 0.0 0.0 0.1419106887291556 0.0 30 0.0 0.0 0.0 0.18251403026355992 0.0 31 0.0 0.0 0.0 0.23477575699101097 0.0 32 0.0 0.0 0.0 0.26774084615755706 0.0 33 0.0 0.0 0.0 0.3191985463199704 0.0 34 0.0 0.0 0.0 0.3943750301509962 0.0 35 0.0 0.0 0.0 0.5009085500184927 0.0 36 0.0 0.0 0.0 0.7216138421213437 0.0 37 0.0 0.0 0.0 1.0673452650875586 0.0 38 0.0 0.0 0.0 1.5626256291507872 0.0 39 0.0 0.0 0.0 2.173283805296927 0.0 40 0.0 0.0 0.0 2.9041439529162045 0.0 41 0.0 0.0 0.0 3.7732966697219674 0.0 42 0.0 0.0 0.0 4.717625870358757 0.0 43 0.0 0.0 0.0 5.660347017865471 0.0 44 0.0 0.0 0.0 6.574123209030827 0.0 45 0.0 0.0 0.0 7.39061218582662 0.0 46 0.0 0.0 0.0 8.294338044929004 0.0 47 0.0 0.0 0.0 9.155852509366909 0.0 48 0.0 0.0 0.0 9.95907504783958 0.0 49 0.0 0.0 0.0 10.693955328284046 0.0 50 0.0 0.0 0.0 11.392252400019297 0.0 51 0.0 0.0 0.0 12.105021949925225 0.0 52 0.0 0.0 0.0 13.049753163844533 0.0 53 0.0 0.0 0.0 13.99488639104636 0.0 54 0.0 0.0 0.0 14.83348609838069 0.0 55 0.0 0.0 0.0 15.5948992554714 0.0 56 0.0 0.0 0.0 16.302040619422066 0.0 57 0.0 0.0 0.0 17.04777525849454 0.0 58 0.0 0.0 0.0 17.720343480148586 0.0 59 0.0 0.0 0.0 18.370800971264092 0.0 60 0.0 0.0 0.0 18.99834370527602 0.0 61 0.0 0.0 0.0 19.580860951645843 0.0 62 0.0 0.0 0.0 20.176644636338786 0.0 63 0.0 0.0 0.0 20.790116905462558 0.0 64 0.0 0.0 0.0 21.411629440236705 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTACGCT 45 3.8380676E-10 62.21632 13 CGTGCGA 25 2.3885016E-4 55.99469 10 GCGTCAT 20 0.0069372235 52.516144 57 ATTCGTT 20 0.0069427444 52.50558 68 GCCGTAT 100 0.0 52.505577 54 CTCGTAT 20 0.006948268 52.495026 48 CCGTGCG 45 1.6815266E-6 46.66224 9 TGGTCGC 125 0.0 44.795753 49 ACGATAT 55 1.3059798E-7 44.55019 24 TCTTACG 55 1.3080353E-7 44.541233 11 CGCCGTA 160 0.0 43.754654 53 GTCCGTG 40 4.5869412E-5 43.745857 7 CGGTGGT 165 0.0 42.420223 46 ATCTCGG 190 0.0 42.364403 42 TCGGTGG 160 0.0 41.558563 45 TACGCTG 60 2.5907866E-7 40.829464 14 CGACGAT 130 0.0 40.380787 22 TCTCGGT 165 0.0 40.29921 43 GCGAGAT 35 0.0012544568 39.99621 38 CATCTCG 35 0.0012544568 39.99621 41 >>END_MODULE