FastQCFastQC Report
Wed 25 May 2016
SRR1779830_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779830_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248748
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT2899411.655973113351665No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG35411.4235290333992634No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC23670.9515654397221284No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT8060.3240227057101967Illumina PCR Primer Index 10 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC7320.29427372280380143No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTACCTCGTATGCCGT3000.12060398475565633No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTAACTCGTATGCCGT2950.11859391834306206No Hit
CTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC2920.11738787849550548Illumina PCR Primer Index 10 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT150.002221582570.01005645
CAAACCG150.002225129669.9819123
CGTTGTG150.002225129669.9819128
CGTAGCG150.002225129669.9819115
CCGTTGT150.002225129669.9819127
ATCTCGT40400.054.30526437
TCTCGTA40150.054.29480738
TATCTCG40250.053.98604236
CTCGTAT41050.053.95681839
CCGTCTT41000.053.7990347
CGTATGC41800.053.7420941
GCCGTCT41250.053.7276146
CGTCTTC41000.053.6390348
TCGTATG41750.053.63882440
TATGCCG41700.053.61923243
TTATCTC40400.053.61237335
GATTATC40550.053.50034333
TGCCGTC41650.053.45305645
ATGCCGT41700.053.36749644
ATTATCT40600.053.26209334