##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779830_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	248748
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.60436666827472	34.0	34.0	34.0	34.0	34.0
2	33.6658586199688	34.0	34.0	34.0	34.0	34.0
3	33.776183125090455	34.0	34.0	34.0	34.0	34.0
4	33.58080466978629	34.0	34.0	34.0	34.0	34.0
5	33.63837699197582	34.0	34.0	34.0	34.0	34.0
6	37.32536140994098	38.0	38.0	38.0	37.0	38.0
7	37.38092768585074	38.0	38.0	38.0	37.0	38.0
8	37.30735121488414	38.0	38.0	38.0	37.0	38.0
9	37.19423271744898	38.0	38.0	38.0	36.0	38.0
10-11	37.15608768713719	38.0	38.0	38.0	36.5	38.0
12-13	37.31867994918552	38.0	38.0	38.0	36.5	38.0
14-15	37.331059948220684	38.0	38.0	38.0	37.0	38.0
16-17	37.25852067152299	38.0	38.0	38.0	36.0	38.0
18-19	37.12635880489491	38.0	38.0	38.0	36.0	38.0
20-21	37.141460433852735	38.0	38.0	38.0	36.0	38.0
22-23	37.139468458037854	38.0	38.0	38.0	36.0	38.0
24-25	37.319200556386384	38.0	38.0	38.0	36.5	38.0
26-27	37.231177738112464	38.0	38.0	38.0	36.0	38.0
28-29	36.76894688600511	38.0	37.0	38.0	35.0	38.0
30-31	37.056358242076314	38.0	38.0	38.0	36.0	38.0
32-33	37.13724934471835	38.0	38.0	38.0	36.0	38.0
34-35	37.084312235676265	38.0	38.0	38.0	36.0	38.0
36-37	37.0575361409941	38.0	38.0	38.0	36.0	38.0
38-39	36.6180733111422	38.0	37.0	38.0	34.5	38.0
40-41	36.9187310048724	38.0	37.5	38.0	35.5	38.0
42-43	37.21967814816601	38.0	38.0	38.0	36.5	38.0
44-45	36.99646630324666	38.0	38.0	38.0	35.5	38.0
46-47	37.13003923649637	38.0	38.0	38.0	36.0	38.0
48-49	36.78968072105103	38.0	37.0	38.0	34.5	38.0
50-51	36.94382065383441	38.0	37.5	38.0	35.5	38.0
52-53	36.868989901426346	38.0	37.5	38.0	35.0	38.0
54-55	36.88106236030039	38.0	37.5	38.0	35.5	38.0
56-57	36.7590653995208	38.0	37.0	38.0	34.5	38.0
58-59	36.83737758695547	38.0	37.5	38.0	35.5	38.0
60-61	36.529580137327734	38.0	37.0	38.0	34.0	38.0
62-63	36.37787037483719	38.0	37.0	38.0	34.0	38.0
64-65	36.741656214321324	38.0	37.0	38.0	34.5	38.0
66-67	36.52981531509801	38.0	37.0	38.0	34.0	38.0
68-69	36.59546408413334	38.0	37.0	38.0	34.0	38.0
70-71	35.722208821779475	38.0	37.0	38.0	29.5	38.0
72-73	34.09651333880071	38.0	36.0	38.0	22.0	38.0
74-75	33.8302318008587	38.0	36.0	38.0	21.0	38.0
76	33.58621576856899	38.0	34.0	38.0	19.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	3.0
22	14.0
23	25.0
24	46.0
25	95.0
26	182.0
27	412.0
28	699.0
29	1271.0
30	2025.0
31	3106.0
32	5014.0
33	8097.0
34	13806.0
35	28722.0
36	41442.0
37	143787.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.96320128694953	15.774783832696562	10.853006233661773	36.409008646692136
2	19.02367054207471	17.284561081898143	40.477913390258415	23.21385498576873
3	17.502854294305884	18.562561307025586	38.70302474793767	25.231559650730862
4	12.622815057809511	26.693279945969415	33.09976361618988	27.584141380031195
5	12.28954604660138	30.49913969157541	43.111502404039435	14.09981185778378
6	25.917394310708026	43.86005113608953	17.17722353546561	13.045331017736824
7	25.078392590091177	26.562625629150787	19.439754289481726	28.91922749127631
8	22.302892887581006	42.45501471368614	16.663852573689034	18.57823982504382
9	21.541479730490295	16.583047903902745	17.440542235515462	44.4349301300915
10-11	22.230731503368872	28.587164519915735	28.265352887259393	20.916751089455996
12-13	22.160781192210592	19.954733304388377	29.29008474439995	28.594400759001076
14-15	20.015437310048725	20.292625468345474	20.836147426310966	38.85578979529484
16-17	27.48806020550919	28.915207358451124	20.810619582871016	22.786112853168667
18-19	27.75338897197163	23.96461479087269	21.836356473217876	26.445639763937802
20-21	20.38147040378214	22.24138485535562	21.75454677022529	35.622597970636946
22-23	20.51534082686092	29.22134047308923	21.692234711434864	28.57108398861498
24-25	27.19621622056234	29.501177908388478	21.477732305243098	21.824873565806083
26-27	27.436602505346773	30.342354511393054	22.10791644555936	20.113126537700808
28-29	20.513531767089585	30.10938781417338	22.717770595140465	26.659309823596573
30-31	27.002428160226415	23.767226269155934	28.732492321546303	20.497853249071348
32-33	26.29428176306945	29.55521250422114	24.191953302137104	19.958552430572308
34-35	21.361176773280587	30.0309952240822	28.847669126987956	19.76015887564925
36-37	19.84618971810829	28.806060752247255	31.0842700242816	20.26347950536286
38-39	19.516739833084085	23.630541753099525	30.26034380175921	26.592374612057185
40-41	19.48960393651406	21.168009391030278	30.999847234952643	28.342539437503017
42-43	26.610786146455208	20.57006170978311	30.56644354660395	22.252708597157735
44-45	19.772018267483556	27.58273433354238	31.794627494492417	20.850619904481643
46-47	25.862519497644204	20.95675141106662	25.961414765143843	27.219314326145337
48-49	25.217288179201443	20.462878093492208	24.98934664801325	29.3304870792931
50-51	19.317485047997184	21.29084786651254	31.571593707594108	27.820073377896165
52-53	17.440242568524	21.091035581006306	38.860225517951356	22.60849633251834
54-55	16.99856007465088	21.553832292940346	32.834722311603066	28.612885320805713
56-57	23.756141610504745	21.143631730631927	26.047065401238477	29.05316125762485
58-59	17.734408145948237	21.16072523711763	37.292968208040186	23.811898408893946
60-61	24.06090023283964	27.79920295654062	25.6278426341844	22.512054176435342
62-63	17.231554925556193	36.88713293321671	22.864553487951262	23.01675865327583
64-65	17.254869637066538	38.01744605654388	21.549036124474537	23.178648181915047
66-67	17.093783365873964	37.989692898233955	21.058177014318545	23.858346721573533
68-69	16.99188479096854	38.139874281601976	21.026974978943073	23.841265948486416
70-71	17.315288909795974	37.34789592291718	20.837328798103886	24.499486369182954
72-73	18.3610776035384	34.28568556493767	21.02452754322477	26.32870928829916
74-75	18.974989898258883	33.36770940758566	21.342516368841206	26.31478432531426
76	19.060484713980156	33.97581254724112	21.31599684791174	25.647705890866984
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	3.0
15	8.5
16	9.5
17	8.0
18	21.5
19	48.0
20	69.0
21	77.0
22	106.0
23	175.0
24	313.5
25	412.0
26	531.5
27	770.0
28	991.5
29	1094.0
30	1345.0
31	1958.0
32	2647.0
33	2974.0
34	3421.5
35	4322.0
36	5544.5
37	6314.0
38	11636.0
39	19634.0
40	21183.5
41	18063.0
42	16069.0
43	14929.5
44	13710.5
45	13438.0
46	13245.0
47	13197.5
48	12934.5
49	12204.5
50	11690.0
51	11135.0
52	9456.5
53	7856.0
54	7379.0
55	6947.5
56	6066.0
57	5132.0
58	4648.0
59	4106.0
60	3336.5
61	2844.0
62	2579.0
63	2457.5
64	2245.0
65	1970.5
66	1702.0
67	1617.0
68	1504.5
69	1326.0
70	1165.5
71	1071.0
72	1018.0
73	909.0
74	822.0
75	791.0
76	747.5
77	613.0
78	497.5
79	473.0
80	436.5
81	361.5
82	291.0
83	259.0
84	213.5
85	138.0
86	95.5
87	83.0
88	61.0
89	30.0
90	20.0
91	13.0
92	7.0
93	6.5
94	4.5
95	2.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.039397301686847734
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	8.040265650377089E-4
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0012060398475565632
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.014271471529419332
52-53	0.030553009471432937
54-55	0.04984964703233795
56-57	0.05487481306382363
58-59	0.0339701223728432
60-61	0.0317590493189895
62-63	0.028341936417579237
64-65	0.015477511376975894
66-67	0.0339701223728432
68-69	0.007035232444079952
70-71	0.011457378551787351
72-73	0.019296637560905012
74-75	0.011457378551787351
76	0.009648318780452506
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	248748.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.81364272275556
#Duplication Level	Percentage of deduplicated	Percentage of total
1	34.47780173605352	7.5208644893627286
2	12.858222295939994	5.609693344268095
3	12.738430917233373	8.336147426310966
4	11.105582278247729	9.690128161834467
5	8.750299478446767	9.543795326997603
6	6.190449862700651	8.102175695884991
7	3.9070418901236614	5.9658771125798
8	2.5027183428244966	4.367472301284835
9	1.5314867031569634	3.006657339958512
>10	5.82001806085402	21.106099345522374
>50	0.08108954866294392	1.1188029652499718
>100	0.0276441643169127	0.9829224757585991
>500	0.0036858885755883604	0.6182964285139981
>1k	0.0036858885755883604	2.3750944731213917
>5k	0.0	0.0
>10k+	0.0018429442877941802	11.655973113351665
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT	28994	11.655973113351665	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG	3541	1.4235290333992634	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC	2367	0.9515654397221284	No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT	806	0.3240227057101967	Illumina PCR Primer Index 10 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC	732	0.29427372280380143	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTACCTCGTATGCCGT	300	0.12060398475565633	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTAACTCGTATGCCGT	295	0.11859391834306206	No Hit
CTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC	292	0.11738787849550548	Illumina PCR Primer Index 10 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.007638252367858234	0.0
2	0.0	0.0	0.0	0.007638252367858234	0.0
3	0.0	0.0	0.0	0.007638252367858234	0.0
4	0.0	0.0	0.0	0.007638252367858234	0.0
5	0.0	0.0	0.0	0.009246305497933652	0.0
6	0.0	0.0	0.0	0.009246305497933652	0.0
7	0.0	0.0	0.0	0.009246305497933652	0.0
8	0.0	0.0	0.0	0.009246305497933652	0.0
9	0.0	0.0	0.0	0.015276504735716468	0.0
10	0.0	0.0	0.0	0.01809059771334845	0.0
11	0.0	0.0	0.0	0.020100664125942722	0.0
12	0.0	0.0	0.0	0.02170871725601814	0.0
13	0.0	0.0	0.0	0.022914757103574702	0.0
14	0.0	0.0	0.0	0.022914757103574702	0.0
15	0.0	0.0	0.0	0.02452281023365012	0.0
16	0.0	0.0	0.0	0.024924823516168974	0.0
17	0.0	0.0	0.0	0.02532683679868783	0.0
18	0.0	0.0	0.0	0.026934889928763247	0.0
19	0.0	0.0	0.0	0.02814092977631981	0.0
20	0.0	0.0	0.0	0.031357036036470644	0.0
21	0.0	0.0	0.0	0.035779182144178044	0.0
22	0.0	0.0	0.0	0.03738723527425346	0.0
23	0.0	0.0	0.0	0.042211394664479714	0.0
24	0.0	0.0	0.0	0.04422146107707399	0.0
25	0.0	0.0	0.0	0.04502548764211169	0.0
26	0.0	0.0	0.0	0.04502548764211169	0.0
27	0.0	0.0	0.0	0.07959862993873318	0.0
28	0.0	0.0	0.0	0.10613150658497757	0.0
29	0.0	0.0	0.0	0.1447247817067876	0.0
30	0.0	0.0	0.0	0.18854422950134272	0.0
31	0.0	0.0	0.0	0.24362004920642577	0.0
32	0.0	0.0	0.0	0.27980124463312267	0.0
33	0.0	0.0	0.0	0.33367102449064917	0.0
34	0.0	0.0	0.0	0.4144756942769389	0.0
35	0.0	0.0	0.0	0.5306575329248878	0.0
36	0.0	0.0	0.0	0.7730715422837571	0.0
37	0.0	0.0	0.0	1.1590042935018574	0.0
38	0.0	0.0	0.0	1.6808175342113303	0.0
39	0.0	0.0	0.0	2.310772347918375	0.0
40	0.0	0.0	0.0	3.085049930049689	0.0
41	0.0	0.0	0.0	4.008072426712979	0.0
42	0.0	0.0	0.0	5.016723752552784	0.0
43	0.0	0.0	0.0	6.02175695884992	0.0
44	0.0	0.0	0.0	6.988196890025247	0.0
45	0.0	0.0	0.0	7.837248942705067	0.0
46	0.0	0.0	0.0	8.812533166095808	0.0
47	0.0	0.0	0.0	9.725505330696127	0.0
48	0.0	0.0	0.0	10.588627848264107	0.0
49	0.0	0.0	0.0	11.379789988261212	0.0
50	0.0	0.0	0.0	12.11748436168331	0.0
51	0.0	0.0	0.0	12.860806921060671	0.0
52	0.0	0.0	0.0	13.821216652998215	0.0
53	0.0	0.0	0.0	14.786450544325984	0.0
54	0.0	0.0	0.0	15.639120716548474	0.0
55	0.0	0.0	0.0	16.40616205959445	0.0
56	0.0	0.0	0.0	17.12898194156335	0.0
57	0.0	0.0	0.0	17.88999308537154	0.0
58	0.0	0.0	0.0	18.569395532828405	0.0
59	0.0	0.0	0.0	19.23432550211459	0.0
60	0.0	0.0	0.0	19.876742727579717	0.0
61	0.0	0.0	0.0	20.474134465402738	0.0
62	0.0	0.0	0.0	21.089214787656584	0.0
63	0.0	0.0	0.0	21.72238570762378	0.0
64	0.0	0.0	0.0	22.376863331564476	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACGT	15	0.0022215825	70.010056	45
CAAACCG	15	0.0022251296	69.98191	23
CGTTGTG	15	0.0022251296	69.98191	28
CGTAGCG	15	0.0022251296	69.98191	15
CCGTTGT	15	0.0022251296	69.98191	27
ATCTCGT	4040	0.0	54.305264	37
TCTCGTA	4015	0.0	54.294807	38
TATCTCG	4025	0.0	53.986042	36
CTCGTAT	4105	0.0	53.956818	39
CCGTCTT	4100	0.0	53.79903	47
CGTATGC	4180	0.0	53.74209	41
GCCGTCT	4125	0.0	53.72761	46
CGTCTTC	4100	0.0	53.63903	48
TCGTATG	4175	0.0	53.638824	40
TATGCCG	4170	0.0	53.619232	43
TTATCTC	4040	0.0	53.612373	35
GATTATC	4055	0.0	53.500343	33
TGCCGTC	4165	0.0	53.453056	45
ATGCCGT	4170	0.0	53.367496	44
ATTATCT	4060	0.0	53.262093	34
>>END_MODULE