FastQCFastQC Report
Wed 25 May 2016
SRR1779829_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779829_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44298
Sequences flagged as poor quality0
Sequence length76
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCATT252.7960796E-670.0000158
GTATCAT252.7960796E-670.0000157
ATCTAGG150.002210755870.042
CACATTT150.002210755870.07
ATGTGCT150.002210755870.034
TAAGTGT150.002210755870.033
GTAGCTC150.002210755870.038
GTGTCGA150.002210755870.036
GATATGC301.0016993E-770.026
GGCGGTC150.002210755870.047
TTACGCT150.002210755870.013
ATTATTT207.8448094E-570.058
CATCAGA207.8448094E-570.09
AAGTGTA207.8448094E-570.034
TAGTCCA150.002210755870.020
GTTATAC301.0016993E-770.01
AGTGTAG550.063.6363635
ATGTATA501.2732926E-1163.00000428
CGTATCA401.0359145E-861.2556
TGGTCGC401.0359145E-861.2549
Produced by FastQC (version 0.11.5)