##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779829_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	44298
Sequences flagged as poor quality	0
Sequence length	76
%GC	50
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.924624136529864	23.0	12.0	33.0	12.0	34.0
2	22.8570364350535	23.0	12.0	34.0	12.0	34.0
3	23.26685177660391	23.0	11.0	34.0	11.0	34.0
4	23.75086911372974	23.0	12.0	34.0	12.0	34.0
5	23.03727030565714	23.0	11.0	34.0	11.0	34.0
6	25.236940719671317	26.0	12.0	37.0	11.0	38.0
7	25.52875976342047	27.0	12.0	37.0	11.0	38.0
8	25.540679037428326	27.0	11.0	37.0	11.0	38.0
9	25.75093683687751	27.0	12.0	37.0	11.0	38.0
10-11	24.57943925233645	26.0	11.5	36.0	11.0	38.0
12-13	24.661451984288227	26.0	11.0	37.0	11.0	38.0
14-15	23.907106415639532	25.5	11.0	35.0	11.0	38.0
16-17	24.000169307869427	24.5	11.0	36.0	11.0	38.0
18-19	23.983238520926452	24.5	11.0	37.0	11.0	38.0
20-21	23.994198383674206	24.5	11.0	35.0	11.0	38.0
22-23	24.205573615061628	24.5	11.0	36.5	10.5	38.0
24-25	24.409104248498803	26.0	11.0	37.0	10.0	38.0
26-27	23.615558264481468	23.0	11.0	36.0	10.0	38.0
28-29	23.81176351076798	23.0	11.0	36.5	11.0	38.0
30-31	23.90809968847352	24.0	11.0	37.0	11.0	38.0
32-33	24.14621427603955	26.0	11.0	37.0	11.0	38.0
34-35	24.24261817689286	26.0	11.0	37.0	11.0	38.0
36-37	24.00725766400289	25.0	11.0	36.5	11.0	38.0
38-39	23.845625084653932	24.0	11.0	36.5	11.0	38.0
40-41	23.826199828434692	24.0	11.0	36.5	11.0	38.0
42-43	24.08475551943654	25.0	11.0	37.0	11.0	38.0
44-45	24.188879859135852	25.5	11.0	37.0	11.0	38.0
46-47	24.018431983385256	25.0	11.0	37.0	11.0	38.0
48-49	23.726748385931643	24.0	11.0	36.5	11.0	38.0
50-51	23.43647568738995	23.0	11.0	36.0	11.0	38.0
52-53	23.828999051875932	24.0	11.0	36.5	11.0	38.0
54-55	23.851516998510093	24.0	11.0	36.5	11.0	38.0
56-57	23.836459885322135	23.5	11.0	37.0	11.0	38.0
58-59	23.712289493882345	23.5	11.0	37.0	11.0	38.0
60-61	23.976330759853717	24.0	11.0	37.0	11.0	38.0
62-63	23.72005508149352	23.5	11.0	37.0	11.0	38.0
64-65	23.652399656869385	23.5	11.0	37.0	11.0	38.0
66-67	23.973452526073412	24.0	11.0	37.0	11.0	38.0
68-69	23.76167095579936	24.0	11.0	36.0	11.0	38.0
70-71	24.042609147139828	24.5	11.0	36.0	11.0	38.0
72-73	23.991037970111517	24.0	11.0	36.0	11.0	38.0
74-75	23.573716646349723	23.5	11.0	36.0	11.0	38.0
76	23.25621924240372	23.0	11.0	34.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	29.0
13	385.0
14	1521.0
15	2718.0
16	3723.0
17	4442.0
18	4483.0
19	4089.0
20	2894.0
21	2049.0
22	1389.0
23	953.0
24	735.0
25	559.0
26	430.0
27	385.0
28	364.0
29	421.0
30	417.0
31	465.0
32	648.0
33	746.0
34	1063.0
35	1451.0
36	2726.0
37	5213.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.852589281683144	19.0595512212741	9.138110072689512	36.94974942435324
2	13.673303535148312	22.000993272833988	28.07801706623324	36.24768612578446
3	13.03444850783331	20.930967538037834	40.05824190708384	25.976342047045016
4	9.853718000812679	42.77619757099643	19.556187638268092	27.813896789922794
5	10.840218520023477	32.63127003476455	35.81651541830331	20.711996026908665
6	15.242223125197526	40.50069980586031	13.605580387376406	30.65149668156576
7	14.673679933178319	37.71587240670925	13.92193602275549	33.68851163735693
8	12.67777326290126	41.48042801029392	12.032145920809066	33.80965280599575
9	19.334010610678405	20.69759566542499	13.924822214696919	46.04357150919968
10-11	14.738814393426338	33.28141225337487	20.9580567971466	31.02171655605219
12-13	14.68915075172694	24.445798907399883	24.910831188766988	35.954219152106184
14-15	26.353334236308633	28.1796017878911	17.82586121269583	27.641202763104427
16-17	28.652858513460128	24.55443309441842	16.578813702804897	30.21389468931655
18-19	19.41757435521192	24.626671934082058	21.596026863818498	34.359726846887526
20-21	25.446972775294597	23.534922569867714	18.70513341460111	32.312971240236585
22-23	28.9426159194546	23.011196893764954	16.863063795205203	31.18312339157524
24-25	18.90872971081563	32.483012393615816	16.37469806081676	32.23355983475179
26-27	23.697458124520292	32.83895435459841	18.33604225924421	25.127545261637096
28-29	19.588676178435975	29.922566371681413	22.539281199205348	27.949476250677264
30-31	21.5224163619125	27.163754571312477	22.63194726624227	28.681881800532754
32-33	17.19377850015802	30.96302316131654	23.128583683236265	28.71461465528918
34-35	15.9265864505497	35.730410637289204	20.542023161839403	27.80097975032169
36-37	23.9247251134542	24.991533268609874	21.695152514054776	29.388589103881152
38-39	24.54425393098622	22.25056721337382	25.495817859601992	27.709360996037972
40-41	19.416226466206147	29.58598582328773	21.7955663912592	29.202221319246917
42-43	13.840353966319022	28.675109485755563	24.75055307237347	32.733983475551945
44-45	14.210086689543076	24.11617301787972	29.696812353259887	31.976927939317317
46-47	17.423695141773525	27.9697941123352	24.800207693696947	29.806303052194327
48-49	18.477132150435686	29.001309314190255	22.497629689827985	30.02392884554607
50-51	25.613472695667877	28.203941576178977	17.624218344357406	28.55836738379574
52-53	12.919357023524631	32.383618548787645	22.159886214837226	32.5371382128505
54-55	15.857141244595951	26.526396586560715	22.36908941088824	35.2473727579551
56-57	19.23532798266111	29.17470960750449	19.15179428132796	32.43816812850644
58-59	17.04558282347098	29.528367913665814	20.525816720475017	32.90023254238819
60-61	14.08895913298713	26.62339128471438	21.776924813727703	37.51072476857079
62-63	17.2976633931595	27.9218873462016	20.449260638898295	34.33118862174061
64-65	17.900927953760355	26.413944142150775	20.079700164819037	35.60542773926983
66-67	14.455695916731578	35.211840010837534	16.535148621036114	33.797315451394766
68-69	16.017653167185877	38.00284437220642	15.15756919048264	30.82193327012506
70-71	13.628308595293188	35.0516394830408	16.096845194424063	35.223206727241944
72-73	12.756927591850555	31.93408205880693	16.49303008070433	38.815960268638186
74-75	13.351544774181894	30.74083689848627	21.001478738895347	34.90613958843649
76	11.251326274917037	35.304873919227035	17.786757568232613	35.657042237623315
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	1.0
15	0.5
16	0.5
17	1.0
18	1.0
19	5.5
20	18.5
21	27.0
22	30.0
23	36.0
24	44.5
25	50.0
26	60.5
27	78.0
28	103.0
29	121.0
30	150.0
31	214.5
32	284.5
33	319.0
34	396.5
35	524.5
36	683.0
37	791.0
38	909.0
39	1134.5
40	1373.5
41	1645.0
42	1785.0
43	1906.5
44	2169.5
45	2434.5
46	2558.0
47	2642.0
48	2763.0
49	2852.5
50	2905.0
51	2857.0
52	2658.5
53	2436.5
54	2365.0
55	2251.5
56	2013.0
57	1757.0
58	1626.0
59	1500.0
60	1250.0
61	1038.0
62	950.0
63	877.0
64	715.0
65	539.0
66	431.5
67	411.0
68	333.5
69	254.0
70	229.0
71	206.0
72	162.5
73	104.5
74	86.5
75	83.0
76	72.0
77	64.0
78	44.5
79	22.0
80	26.0
81	26.0
82	19.0
83	16.0
84	15.0
85	11.5
86	9.5
87	10.0
88	9.0
89	9.5
90	8.0
91	4.5
92	4.0
93	3.0
94	2.5
95	3.5
96	4.0
97	3.5
98	4.5
99	4.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0022574382590636144
8	0.0
9	0.006772314777190844
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0011287191295318072
18-19	0.0011287191295318072
20-21	0.0
22-23	0.0
24-25	0.0022574382590636144
26-27	0.0
28-29	0.004514876518127229
30-31	0.0
32-33	0.0
34-35	0.0022574382590636144
36-37	0.015802067813445304
38-39	0.005643595647659036
40-41	0.0
42-43	0.0
44-45	0.004514876518127229
46-47	0.004514876518127229
48-49	0.0
50-51	0.0022574382590636144
52-53	0.009029753036254458
54-55	0.005643595647659036
56-57	0.010158472165786265
58-59	0.011287191295318072
60-61	0.018059506072508915
62-63	0.006772314777190844
64-65	0.015802067813445304
66-67	0.01693078694297711
68-69	0.0
70-71	0.0011287191295318072
72-73	0.0011287191295318072
74-75	0.007901033906722652
76	0.0022574382590636144
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	44298.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.85507246376811
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.21666164204603	84.65844959140367
2	2.9519646266706867	5.304979908799495
3	1.48226308913677	3.995665718542598
4	0.6883730278363983	2.474152331933722
5	0.3416742035976284	1.5350580161632579
6	0.1708371017988142	0.9210348096979548
7	0.06783237865541152	0.4266558309630231
8	0.03768465480856195	0.27089259108763375
9	0.012561551602853984	0.10158472165786266
>10	0.030147723846849564	0.3115264797507788
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0067723147771908436	0.0
3	0.0	0.0	0.0	0.0067723147771908436	0.0
4	0.0	0.0	0.0	0.0067723147771908436	0.0
5	0.0	0.0	0.0	0.00902975303625446	0.0
6	0.0	0.0	0.0	0.00902975303625446	0.0
7	0.0	0.0	0.0	0.00902975303625446	0.0
8	0.0	0.0	0.0	0.00902975303625446	0.0
9	0.0	0.0	0.0	0.00902975303625446	0.0
10	0.0	0.0	0.0	0.00902975303625446	0.0
11	0.0	0.0	0.0	0.00902975303625446	0.0
12	0.0	0.0	0.0	0.015802067813445304	0.0
13	0.0	0.0	0.0	0.015802067813445304	0.0
14	0.0	0.0	0.0	0.015802067813445304	0.0
15	0.0	0.0	0.0	0.02031694433157253	0.0
16	0.0	0.0	0.0	0.02031694433157253	0.0
17	0.0	0.0	0.0	0.022574382590636145	0.0
18	0.0	0.0	0.0	0.02483182084969976	0.0
19	0.0	0.0	0.0	0.03611901214501784	0.0
20	0.0	0.0	0.0	0.03611901214501784	0.0
21	0.0	0.0	0.0	0.04966364169939952	0.0
22	0.0	0.0	0.0	0.08126777732629012	0.0
23	0.0	0.0	0.0	0.08352521558535374	0.0
24	0.0	0.0	0.0	0.10158472165786266	0.0
25	0.0	0.0	0.0	0.10609959817598989	0.0
26	0.0	0.0	0.0	0.1083570364350535	0.0
27	0.0	0.0	0.0	0.13093141902568964	0.0
28	0.0	0.0	0.0	0.1399611720619441	0.0
29	0.0	0.0	0.0	0.16705043117070748	0.0
30	0.0	0.0	0.0	0.1828524989841528	0.0
31	0.0	0.0	0.0	0.18736737550228003	0.0
32	0.0	0.0	0.0	0.2031694433157253	0.0
33	0.0	0.0	0.0	0.22574382590636147	0.0
34	0.0	0.0	0.0	0.26412027631044294	0.0
35	0.0	0.0	0.0	0.32055623278703327	0.0
36	0.0	0.0	0.0	0.4289132692220868	0.0
37	0.0	0.0	0.0	0.5733893178021581	0.0
38	0.0	0.0	0.0	0.7584992550453745	0.0
39	0.0	0.0	0.0	0.9774707661745451	0.0
40	0.0	0.0	0.0	1.1670955799358889	0.0
41	0.0	0.0	0.0	1.4447604858007133	0.0
42	0.0	0.0	0.0	1.7179105151474108	0.0
43	0.0	0.0	0.0	2.06104113052508	0.0
44	0.0	0.0	0.0	2.4109440606799404	0.0
45	0.0	0.0	0.0	2.812768070793264	0.0
46	0.0	0.0	0.0	3.151383809652806	0.0
47	0.0	0.0	0.0	3.501286739807666	0.0
48	0.0	0.0	0.0	3.7766942074134273	0.0
49	0.0	0.0	0.0	4.047586798501061	0.0
50	0.0	0.0	0.0	4.438123617319066	0.0
51	0.0	0.0	0.0	4.742877782292655	0.0
52	0.0	0.0	0.0	5.0160278116393515	0.0
53	0.0	0.0	0.0	5.286920402726985	0.0
54	0.0	0.0	0.0	5.569100185109937	0.0
55	0.0	0.0	0.0	5.849022529233825	0.0
56	0.0	0.0	0.0	6.054449410808615	0.0
57	0.0	0.0	0.0	6.417896970517856	0.0
58	0.0	0.0	0.0	6.643640796424218	0.0
59	0.0	0.0	0.0	6.855839992776198	0.0
60	0.0	0.0	0.0	7.092871009977877	0.0
61	0.0	0.0	0.0	7.2373470585579485	0.0
62	0.0	0.0	0.0	7.440516501873674	0.0
63	0.0	0.0	0.0	7.6391710686712715	0.0
64	0.0	0.0	0.0	7.828795882432615	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCATT	25	2.7960796E-6	70.00001	58
GTATCAT	25	2.7960796E-6	70.00001	57
ATCTAGG	15	0.0022107558	70.0	42
CACATTT	15	0.0022107558	70.0	7
ATGTGCT	15	0.0022107558	70.0	34
TAAGTGT	15	0.0022107558	70.0	33
GTAGCTC	15	0.0022107558	70.0	38
GTGTCGA	15	0.0022107558	70.0	36
GATATGC	30	1.0016993E-7	70.0	26
GGCGGTC	15	0.0022107558	70.0	47
TTACGCT	15	0.0022107558	70.0	13
ATTATTT	20	7.8448094E-5	70.0	58
CATCAGA	20	7.8448094E-5	70.0	9
AAGTGTA	20	7.8448094E-5	70.0	34
TAGTCCA	15	0.0022107558	70.0	20
GTTATAC	30	1.0016993E-7	70.0	1
AGTGTAG	55	0.0	63.63636	35
ATGTATA	50	1.2732926E-11	63.000004	28
CGTATCA	40	1.0359145E-8	61.25	56
TGGTCGC	40	1.0359145E-8	61.25	49
>>END_MODULE