SRR1779829_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1779829_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	44298
Sequences flagged as poor quality	0
Sequence length	76
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT	20539	46.36552440290758	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG	2639	5.957379565668879	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC	1360	3.0701160323265158	No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT	548	1.237076165966861	RNA PCR Primer, Index 24 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC	483	1.0903426791277258	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT	310	0.6998058603097206	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGT	285	0.6433699038331302	No Hit
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC	249	0.5621021265068401	No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG	164	0.3702198744864328	No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC	155	0.3499029301548603	RNA PCR Primer, Index 24 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAGCTCGTATGCCGT	130	0.2934669736782699	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATATCGTATGCCGT	128	0.28895209716014264	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCG	105	0.23703101720167954	No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG	94	0.21219919635197976	No Hit
TTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC	87	0.19639712853853447	No Hit
CCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG	72	0.16253555465258024	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC	60	0.13544629554381687	No Hit
TATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCT	58	0.13093141902568964	RNA PCR Primer, Index 24 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCAGT	56	0.12641654250756243	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCG	55	0.1241591042484988	No Hit
CAGCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGC	46	0.10384215991692627	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTCTGCCGT	45	0.10158472165786266	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATGC	15	0.0022107558	70.0	51
TCTTTAT	55	0.0	70.0	1
CGTCTTA	20	7.8448094E-5	70.0	48
GAAAAAC	40	1.0359145E-8	61.25	60
TGCAAAA	25	2.365914E-4	56.000004	59
GCAAAAA	25	2.365914E-4	56.000004	60
TGCTTGC	25	2.365914E-4	56.000004	55
ATCGTAT	25	2.365914E-4	56.000004	39
CTTTATA	70	0.0	55.0	2
TTTATAC	70	0.0	55.0	3
CATCTCG	2790	0.0	53.44086	36
AGCATCT	2790	0.0	53.44086	34
GCATCTC	2795	0.0	53.34526	35
ATCTCGT	2810	0.0	53.309605	37
TAGCATC	2810	0.0	53.309605	33
TCTCGTA	2810	0.0	53.309605	38
ATAGCAT	2840	0.0	53.1162	32
TCGTATG	2980	0.0	53.087246	40
GTATGCC	2995	0.0	53.055096	42
CGTATGC	2995	0.0	53.055096	41

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content