##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779829_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	44298
Sequences flagged as poor quality	0
Sequence length	76
%GC	44
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.57307327644589	34.0	34.0	34.0	34.0	34.0
2	33.69249176035036	34.0	34.0	34.0	34.0	34.0
3	33.7933992505305	34.0	34.0	34.0	34.0	34.0
4	33.57388595421915	34.0	34.0	34.0	34.0	34.0
5	33.646056255361415	34.0	34.0	34.0	34.0	34.0
6	37.3878956160549	38.0	38.0	38.0	37.0	38.0
7	37.41205020542688	38.0	38.0	38.0	37.0	38.0
8	37.31222628561109	38.0	38.0	38.0	37.0	38.0
9	37.26147907354734	38.0	38.0	38.0	37.0	38.0
10-11	37.18593390220778	38.0	38.0	38.0	36.5	38.0
12-13	37.35230710190076	38.0	38.0	38.0	36.5	38.0
14-15	37.364226375908615	38.0	38.0	38.0	37.0	38.0
16-17	37.158460878594965	38.0	38.0	38.0	36.0	38.0
18-19	36.970190527789065	38.0	38.0	38.0	35.0	38.0
20-21	37.056729423450264	38.0	38.0	38.0	35.5	38.0
22-23	37.17507562418168	38.0	38.0	38.0	36.0	38.0
24-25	37.19404939274911	38.0	38.0	38.0	36.0	38.0
26-27	37.02598311436182	38.0	38.0	38.0	36.0	38.0
28-29	36.434714885547876	38.0	37.0	38.0	34.0	38.0
30-31	36.61783827712312	38.0	37.0	38.0	34.5	38.0
32-33	36.81487877556549	38.0	37.0	38.0	35.0	38.0
34-35	36.78844417355185	38.0	37.0	38.0	35.0	38.0
36-37	36.6224660255542	38.0	37.0	38.0	34.5	38.0
38-39	33.941103435821034	37.0	34.0	38.0	24.0	38.0
40-41	35.90203846674794	37.5	36.5	38.0	32.5	38.0
42-43	37.016784053456135	38.0	37.5	38.0	35.5	38.0
44-45	36.77914352792451	38.0	37.0	38.0	35.0	38.0
46-47	36.9443089981489	38.0	37.5	38.0	35.5	38.0
48-49	36.45127319517811	38.0	37.0	38.0	34.0	38.0
50-51	36.44809020723283	38.0	37.0	38.0	33.5	38.0
52-53	36.392354056616554	38.0	37.0	38.0	34.0	38.0
54-55	36.43858639216218	38.0	37.0	38.0	34.0	38.0
56-57	36.29860264571764	38.0	37.0	38.0	34.0	38.0
58-59	36.25741568468102	38.0	37.0	38.0	34.0	38.0
60-61	35.72222222222223	38.0	37.0	38.0	31.5	38.0
62-63	35.76670504311707	38.0	37.0	38.0	32.5	38.0
64-65	36.360298433337846	38.0	37.0	38.0	34.0	38.0
66-67	35.94354146914082	38.0	36.5	38.0	32.5	38.0
68-69	35.68994085511761	38.0	37.0	38.0	31.0	38.0
70-71	32.01743871055127	36.0	29.0	38.0	18.5	38.0
72-73	25.290272698541692	24.0	16.0	37.5	10.0	38.0
74-75	24.196205246286514	23.5	11.0	37.0	11.0	38.0
76	24.324145559618945	24.0	11.0	37.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	0.0
22	0.0
23	8.0
24	12.0
25	25.0
26	73.0
27	136.0
28	256.0
29	444.0
30	758.0
31	1129.0
32	1745.0
33	2731.0
34	4997.0
35	11816.0
36	10487.0
37	9680.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.515222694010298	7.9998193152046255	12.799259192338965	65.68569879844611
2	6.523996568693846	7.165109034267912	69.2762652941442	17.034629102894037
3	6.747482956341145	9.700212199196352	70.90613571718814	12.646169127274371
4	4.503589326831911	58.00036119012145	25.849925504537453	11.646123978509188
5	4.2665583096302315	22.20190527789065	68.18818005327554	5.343356359203575
6	9.26226917693801	68.90153054313964	14.230890785137026	7.605309494785317
7	8.185471127364668	20.4862522010023	13.1405481060093	58.18772856562373
8	7.727211160774752	68.25816063930652	8.645988532213645	15.36863966770509
9	7.99358887534426	14.99616235495959	7.214772675967312	69.79547609372884
10-11	8.223847577768748	40.8551176125333	35.72847532619983	15.192559483498128
12-13	8.764504040814483	10.168630637952052	41.45898234683281	39.607882974400646
14-15	8.796108176441374	8.745315815612443	14.211702559934986	68.24687344801119
16-17	37.769199512393335	35.09074901801436	8.473294505395277	18.666756964197027
18-19	39.83814167682514	16.089891191475914	8.504898641022168	35.56706849067678
20-21	10.60431622195133	9.16181317440968	8.655018285249898	71.5788523183891
22-23	9.022980721477268	39.00853311661926	8.354778996794437	43.613707165109034
24-25	37.94839379641962	38.5161523353726	8.402374878659954	15.133078989547824
26-27	40.631631224886	41.883380739536776	9.318705133414602	8.166282902162626
28-29	37.44412840308818	15.896880220325974	9.489141721973905	37.169849654611944
30-31	40.348322723373514	10.153957289268138	39.298613932908935	10.19910605444941
32-33	14.710596415188046	39.23992053817328	37.8211205923518	8.228362454286875
34-35	33.95525757370536	41.707300555329816	16.114723012325612	8.222718858639217
36-37	14.236534380784684	37.5942480473159	10.094135175402954	38.075082396496455
38-39	8.370581064607883	15.874305837735339	37.77258566978193	37.98252742787484
40-41	8.08162896744774	9.160684455280148	42.03011422637591	40.7275723508962
42-43	37.88249901235962	8.670918223376036	39.10717309103223	14.339409673232122
44-45	10.341324664770418	38.13829066775023	42.07864914894577	9.44173551853357
46-47	37.71614971330534	10.549008984604272	16.645221003205563	35.089620298884824
48-49	36.04903155898686	7.749785543365389	10.962120186012912	45.23906271163484
50-51	13.489563931502365	8.33982412769368	40.15600483140867	38.014607109395286
52-53	7.317128244498888	8.154855317083085	69.41392975285923	15.114086685558807
54-55	6.977505759067709	8.375491214598672	46.014950991463024	38.63205203487059
56-57	36.70411562129509	8.401738835883249	17.313837294642354	37.58030824817931
58-59	10.184840170274267	9.70043923534659	65.39525535494506	14.719465239434076
60-61	36.235336622596556	36.74227455939303	18.232830158855606	8.789558659154803
62-63	8.74051661849711	73.01977962427746	8.98211705202312	9.257586705202312
64-65	6.473025298307802	76.28551753722498	8.263436551031237	8.978020613435987
66-67	6.478418443960213	76.19085253638323	7.995845141186167	9.334883878470379
68-69	6.334872275338925	75.93945072187292	8.107101333122623	9.618575669665534
70-71	7.205183487791938	70.86319972005553	8.759552540383117	13.172064251769408
72-73	9.395003330360469	58.336626062610776	11.227266056288737	21.041104550740016
74-75	11.067336456510695	52.5371112490828	12.65112603713947	23.744426257267033
76	10.780484500936943	54.019822545323194	12.315715801593932	22.88397715214594
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	2.5
17	5.0
18	2.5
19	4.5
20	9.0
21	9.0
22	12.5
23	30.0
24	49.5
25	55.0
26	66.0
27	66.0
28	82.5
29	110.0
30	126.5
31	144.5
32	175.0
33	204.0
34	249.5
35	334.5
36	412.5
37	451.0
38	493.0
39	720.0
40	4210.0
41	8833.0
42	10151.0
43	9029.5
44	6335.0
45	3705.0
46	2648.0
47	2085.0
48	1275.5
49	965.5
50	902.0
51	811.5
52	634.5
53	540.0
54	532.0
55	503.5
56	446.0
57	363.5
58	310.0
59	268.0
60	214.5
61	177.0
62	151.0
63	137.5
64	122.5
65	116.0
66	102.5
67	94.0
68	82.5
69	71.0
70	70.5
71	70.0
72	55.0
73	33.0
74	23.5
75	21.0
76	22.5
77	20.5
78	18.0
79	19.0
80	13.0
81	5.5
82	5.0
83	6.0
84	8.0
85	7.0
86	3.0
87	2.0
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04966364169939952
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0022574382590636144
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0011287191295318072
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.010158472165786265
52-53	0.02596053997923157
54-55	0.04514876518127229
56-57	0.03499029301548603
58-59	0.03724773127454964
60-61	0.028217978238295187
62-63	0.022574382590636145
64-65	0.014673348683913495
66-67	0.028217978238295187
68-69	0.007901033906722652
70-71	0.007901033906722652
72-73	0.019188225202040724
74-75	0.01241591042484988
76	0.011287191295318072
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	44298.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.316763736511806
#Duplication Level	Percentage of deduplicated	Percentage of total
1	52.75318206972883	8.607612081809563
2	16.380741560597674	5.345613797462639
3	11.676812396236857	5.715833671949072
4	7.941339236303264	5.183078242810059
5	4.703929164360819	3.837645040408145
6	2.794687327061428	2.7360151699851007
7	1.3004980630879912	1.4853943744638585
8	0.6640841173215274	0.8668562914804281
9	0.3043718871057	0.4469727752945957
>10	1.2036524626452685	3.5216036841392384
>50	0.09684560044272275	1.0880852408686623
>100	0.12451577199778638	4.5351934624588015
>500	0.01383508577753182	1.237076165966861
>1k	0.02767017155506364	9.027495597995394
>5k	0.0	0.0
>10k+	0.01383508577753182	46.36552440290758
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT	20539	46.36552440290758	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG	2639	5.957379565668879	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC	1360	3.0701160323265158	No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT	548	1.237076165966861	RNA PCR Primer, Index 24 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC	483	1.0903426791277258	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT	310	0.6998058603097206	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGT	285	0.6433699038331302	No Hit
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC	249	0.5621021265068401	No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG	164	0.3702198744864328	No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC	155	0.3499029301548603	RNA PCR Primer, Index 24 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAGCTCGTATGCCGT	130	0.2934669736782699	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATATCGTATGCCGT	128	0.28895209716014264	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCG	105	0.23703101720167954	No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG	94	0.21219919635197976	No Hit
TTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC	87	0.19639712853853447	No Hit
CCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG	72	0.16253555465258024	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC	60	0.13544629554381687	No Hit
TATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCT	58	0.13093141902568964	RNA PCR Primer, Index 24 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCAGT	56	0.12641654250756243	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCG	55	0.1241591042484988	No Hit
CAGCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGC	46	0.10384215991692627	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTCTGCCGT	45	0.10158472165786266	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0067723147771908436	0.0
3	0.0	0.0	0.0	0.0067723147771908436	0.0
4	0.0	0.0	0.0	0.013544629554381687	0.0
5	0.0	0.0	0.0	0.01805950607250892	0.0
6	0.0	0.0	0.0	0.01805950607250892	0.0
7	0.0	0.0	0.0	0.01805950607250892	0.0
8	0.0	0.0	0.0	0.01805950607250892	0.0
9	0.0	0.0	0.0	0.01805950607250892	0.0
10	0.0	0.0	0.0	0.01805950607250892	0.0
11	0.0	0.0	0.0	0.01805950607250892	0.0
12	0.0	0.0	0.0	0.02483182084969976	0.0
13	0.0	0.0	0.0	0.02483182084969976	0.0
14	0.0	0.0	0.0	0.02483182084969976	0.0
15	0.0	0.0	0.0	0.02934669736782699	0.0
16	0.0	0.0	0.0	0.02934669736782699	0.0
17	0.0	0.0	0.0	0.03386157388595422	0.0
18	0.0	0.0	0.0	0.03611901214501784	0.0
19	0.0	0.0	0.0	0.04740620344033591	0.0
20	0.0	0.0	0.0	0.04740620344033591	0.0
21	0.0	0.0	0.0	0.05643595647659037	0.0
22	0.0	0.0	0.0	0.060950832994717596	0.0
23	0.0	0.0	0.0	0.08126777732629012	0.0
24	0.0	0.0	0.0	0.08352521558535374	0.0
25	0.0	0.0	0.0	0.08804009210348097	0.0
26	0.0	0.0	0.0	0.09029753036254458	0.0
27	0.0	0.0	0.0	0.11061447469411712	0.0
28	0.0	0.0	0.0	0.11964422773037157	0.0
29	0.0	0.0	0.0	0.14673348683913495	0.0
30	0.0	0.0	0.0	0.16253555465258024	0.0
31	0.0	0.0	0.0	0.16705043117070748	0.0
32	0.0	0.0	0.0	0.18059506072508916	0.0
33	0.0	0.0	0.0	0.2031694433157253	0.0
34	0.0	0.0	0.0	0.24154589371980675	0.0
35	0.0	0.0	0.0	0.3092690414917152	0.0
36	0.0	0.0	0.0	0.44245789877646846	0.0
37	0.0	0.0	0.0	0.6298252742787485	0.0
38	0.0	0.0	0.0	0.8036480202266468	0.0
39	0.0	0.0	0.0	1.0203620930967539	0.0
40	0.0	0.0	0.0	1.2077294685990339	0.0
41	0.0	0.0	0.0	1.4853943744638585	0.0
42	0.0	0.0	0.0	1.7495146507743013	0.0
43	0.0	0.0	0.0	2.0791006365975893	0.0
44	0.0	0.0	0.0	2.438033319788704	0.0
45	0.0	0.0	0.0	2.8375998916429634	0.0
46	0.0	0.0	0.0	3.1852453835387604	0.0
47	0.0	0.0	0.0	3.553207819766129	0.0
48	0.0	0.0	0.0	3.844417355185336	0.0
49	0.0	0.0	0.0	4.119824822791097	0.0
50	0.0	0.0	0.0	4.526163709422547	0.0
51	0.0	0.0	0.0	4.844462503950517	0.0
52	0.0	0.0	0.0	5.099553027224705	0.0
53	0.0	0.0	0.0	5.365930741794212	0.0
54	0.0	0.0	0.0	5.648110524177164	0.0
55	0.0	0.0	0.0	5.921260553523861	0.0
56	0.0	0.0	0.0	6.122172558580523	0.0
57	0.0	0.0	0.0	6.481105241771638	0.0
58	0.0	0.0	0.0	6.704591629418935	0.0
59	0.0	0.0	0.0	6.912275949252788	0.0
60	0.0	0.0	0.0	7.158336719490722	0.0
61	0.0	0.0	0.0	7.314099959366112	0.0
62	0.0	0.0	0.0	7.501467334868392	0.0
63	0.0	0.0	0.0	7.7181814077384985	0.0
64	0.0	0.0	0.0	7.923608289313287	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATGC	15	0.0022107558	70.0	51
TCTTTAT	55	0.0	70.0	1
CGTCTTA	20	7.8448094E-5	70.0	48
GAAAAAC	40	1.0359145E-8	61.25	60
TGCAAAA	25	2.365914E-4	56.000004	59
GCAAAAA	25	2.365914E-4	56.000004	60
TGCTTGC	25	2.365914E-4	56.000004	55
ATCGTAT	25	2.365914E-4	56.000004	39
CTTTATA	70	0.0	55.0	2
TTTATAC	70	0.0	55.0	3
CATCTCG	2790	0.0	53.44086	36
AGCATCT	2790	0.0	53.44086	34
GCATCTC	2795	0.0	53.34526	35
ATCTCGT	2810	0.0	53.309605	37
TAGCATC	2810	0.0	53.309605	33
TCTCGTA	2810	0.0	53.309605	38
ATAGCAT	2840	0.0	53.1162	32
TCGTATG	2980	0.0	53.087246	40
GTATGCC	2995	0.0	53.055096	42
CGTATGC	2995	0.0	53.055096	41
>>END_MODULE