FastQCFastQC Report
Wed 25 May 2016
SRR1779828_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779828_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404611
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAA305.8814103E-446.6561210
CGACGAA2400.043.74011222
CGACGAT953.6379788E-1240.5171622
CGACCAT350.001256239.9909622
CTTACGC350.001256239.9909612
TCTCGTT459.1968745E-538.88490743
ACGCTTC556.698836E-638.1731915
TCTTACG654.868325E-737.6837911
GCCGTAT852.561137E-937.07335754
CGTACTC400.002415869235.0050737
CTCGTTG501.7095573E-434.99641844
GATATCC400.002418801234.99641826
CTTCCGA806.2464096E-834.99209218
TTACGCT400.002420268334.99209213
TGGTCGC1350.033.70025349
AGATCTC2500.032.196740
TCGCCGT1206.548362E-1132.0879852
CGCTATG552.9918485E-431.81492225
GTCGAGG450.00430995831.1040820
CGACGCA450.00430995831.1040822
Produced by FastQC (version 0.11.5)