##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779828_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	404611
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.797968913351344	34.0	31.0	34.0	12.0	34.0
2	30.673120602257477	34.0	32.0	34.0	21.0	34.0
3	30.164864524197316	34.0	31.0	34.0	11.0	34.0
4	30.272429568153115	34.0	31.0	34.0	12.0	34.0
5	31.11096584126482	34.0	32.0	34.0	23.0	34.0
6	33.790564270373274	38.0	34.0	38.0	21.0	38.0
7	33.97746724631807	38.0	34.0	38.0	21.0	38.0
8	33.14018897162954	38.0	34.0	38.0	11.0	38.0
9	34.05172869744026	38.0	34.0	38.0	21.0	38.0
10-11	32.58975905252205	37.0	30.5	38.0	16.5	38.0
12-13	33.58220241169914	38.0	34.0	38.0	21.0	38.0
14-15	32.304402253028215	37.0	30.0	38.0	14.5	38.0
16-17	32.99657325183942	37.5	32.5	38.0	16.0	38.0
18-19	33.97613510260473	38.0	34.5	38.0	21.5	38.0
20-21	32.52631663499015	37.0	30.5	38.0	16.0	38.0
22-23	33.50160153826762	38.0	34.0	38.0	16.0	38.0
24-25	33.81329968784833	38.0	34.0	38.0	21.0	38.0
26-27	32.680399198242256	37.0	32.5	38.0	11.0	38.0
28-29	34.034629557772774	38.0	35.0	38.0	22.0	38.0
30-31	34.46736495053273	38.0	36.0	38.0	23.0	38.0
32-33	34.57435289698006	38.0	36.0	38.0	24.0	38.0
34-35	34.46004063161901	38.0	36.0	38.0	23.5	38.0
36-37	33.93141066357563	38.0	34.5	38.0	22.0	38.0
38-39	33.89059368133837	38.0	34.5	38.0	22.0	38.0
40-41	33.892145789412545	38.0	34.5	38.0	21.5	38.0
42-43	34.29465832614536	38.0	35.0	38.0	23.0	38.0
44-45	34.35499405601924	38.0	36.0	38.0	23.0	38.0
46-47	34.277293499188104	38.0	35.5	38.0	23.0	38.0
48-49	33.99729122539921	38.0	34.5	38.0	22.0	38.0
50-51	33.29491042013193	37.5	33.0	38.0	16.0	38.0
52-53	33.883214989211865	37.5	34.0	38.0	22.0	38.0
54-55	34.04966622261877	38.0	35.0	38.0	22.0	38.0
56-57	34.112462340371366	38.0	35.0	38.0	22.0	38.0
58-59	34.410654925348055	38.0	36.0	38.0	23.0	38.0
60-61	34.24625751647879	38.0	35.5	38.0	23.0	38.0
62-63	34.28251209186107	38.0	36.0	38.0	23.0	38.0
64-65	34.28240952420967	38.0	35.5	38.0	23.0	38.0
66-67	34.08483333374525	38.0	35.0	38.0	22.5	38.0
68-69	33.41003581217515	38.0	34.0	38.0	21.0	38.0
70-71	33.449919799511136	37.0	34.0	38.0	21.0	38.0
72-73	33.68922125201737	37.0	34.0	38.0	21.5	38.0
74-75	33.75101022957853	37.5	34.0	38.0	21.5	38.0
76	32.69404440314277	37.0	31.0	38.0	19.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	70.0
13	659.0
14	1950.0
15	3233.0
16	4274.0
17	5123.0
18	5483.0
19	5233.0
20	4417.0
21	3680.0
22	3207.0
23	3206.0
24	3451.0
25	3848.0
26	4570.0
27	5425.0
28	6566.0
29	7815.0
30	9747.0
31	11975.0
32	15186.0
33	20068.0
34	27650.0
35	40329.0
36	70484.0
37	136959.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.97377721756754	19.77163194186995	11.429771879093447	27.824818961469067
2	20.584215456327186	21.640044388313715	33.75167753719004	24.024062618169058
3	18.170786261372037	22.386934611268604	31.47368707227436	27.968592055085008
4	13.266569618720203	22.392124781580332	34.07668105909132	30.264624540608136
5	12.988771931558954	35.38262676990986	35.42785539691209	16.200745901619086
6	27.211387077081255	39.26381429514161	18.433560142264316	15.091238485512818
7	26.51576194689828	29.58806867334681	22.51434814203301	21.381821237721894
8	23.088007038981324	37.843175055115616	19.33940346610513	19.729414439797928
9	23.68927767217791	18.81221157434986	21.03878961951914	36.45972113395309
10-11	23.919026298781144	26.519863420831165	25.311634562982498	24.249475717405193
12-13	23.9502532688926	23.655153920014236	26.025464058555226	26.36912875253794
14-15	24.056123457766528	25.071303582726983	23.3131821417273	27.55939081777918
16-17	24.61944741713605	26.600001482913132	24.215600740467956	24.56495035948286
18-19	22.08665606358881	26.46580136653654	26.387328704064146	25.06021386581051
20-21	23.487868594773744	26.231120755491077	26.05032982296576	24.230680826769415
22-23	24.617711682810064	26.06703361072459	25.22707444087167	24.08818026559368
24-25	22.322706297871708	27.783943034674007	25.058267856657366	24.835082810796916
26-27	22.961578908994746	27.811205152635203	25.46881738767228	23.758398550697773
28-29	22.601634255094073	27.181725607284445	26.738558731351397	23.478081406270082
30-31	22.67534829922221	27.059951706462286	26.26869397368818	23.996006020627323
32-33	21.886824629088185	27.850453892751307	26.213820187785302	24.0489012903752
34-35	22.414500498031355	27.579691195843726	26.454362779385704	23.55144552673922
36-37	23.82140129089035	26.2914465536293	25.828065864577642	24.05908629090271
38-39	23.1262443813594	26.431741472704868	26.72810661235366	23.713907533582077
40-41	22.25875973475496	26.812437748234707	26.77820700086751	24.150595516142825
42-43	21.492089855093955	26.552926667111542	27.09616986359603	24.858813614198468
44-45	20.989988642837634	25.612810978013574	28.134743648207262	25.262456730941533
46-47	21.286305874560213	25.794332928407158	27.964992628531103	24.954368568501522
48-49	21.18697163561409	26.401258497390696	27.989209348767147	24.42256051822807
50-51	22.415621199988138	26.152855631679373	26.932012535713934	24.49951063261856
52-53	19.844050677967985	26.795400866879493	27.869792574300867	25.49075588085165
54-55	19.852237813574448	26.449134758224186	27.720364140603955	25.978263287597418
56-57	20.243553964765876	26.567231325593067	27.434612694073184	25.75460201556788
58-59	20.008527941367312	26.694804753400366	27.47060023111957	25.826067074112753
60-61	20.095302843016068	26.081334981458593	27.387391841779973	26.435970333745363
62-63	20.024816747844294	26.368906888995724	27.138868736953597	26.467407626206384
64-65	20.70516980315812	25.990296962393007	26.45282902258892	26.851704211859957
66-67	20.511711266299983	26.93072121624127	25.626104690686606	26.93146282677214
68-69	20.398879903511546	27.675305480860484	25.282742802910473	26.643071812717494
70-71	20.789525716121698	27.073107436790984	25.12851882059267	27.008848026494647
72-73	20.37215544663844	26.29038357444221	25.080760969774264	28.25670000914508
74-75	20.349019002008344	25.76128533910088	25.772284875637265	28.117410783253515
76	19.794166569863002	25.968180840783095	25.550236159753236	28.687416429600667
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	1.0
11	3.5
12	5.0
13	6.5
14	10.0
15	19.5
16	56.0
17	85.0
18	119.0
19	171.5
20	245.5
21	301.0
22	393.0
23	582.0
24	867.0
25	1055.0
26	1371.5
27	1843.0
28	2307.0
29	2616.0
30	3066.0
31	3931.0
32	5306.5
33	6267.0
34	6911.5
35	8542.5
36	10768.0
37	12007.0
38	13236.5
39	16190.5
40	19315.5
41	21520.0
42	22324.0
43	23349.0
44	25207.0
45	26176.5
46	26313.0
47	26312.0
48	25531.0
49	24055.5
50	23360.0
51	22192.0
52	19188.5
53	16181.5
54	15010.0
55	13962.0
56	11771.5
57	9386.0
58	8143.0
59	7295.0
60	5899.0
61	4712.0
62	4073.0
63	3832.5
64	3277.5
65	2785.0
66	2445.0
67	2283.0
68	2098.0
69	1793.0
70	1558.5
71	1444.0
72	1341.5
73	1202.0
74	1093.0
75	1021.0
76	988.0
77	886.5
78	711.5
79	605.0
80	578.5
81	499.5
82	413.0
83	379.0
84	321.5
85	225.0
86	168.5
87	151.0
88	120.5
89	80.5
90	57.5
91	35.5
92	27.0
93	20.0
94	14.0
95	14.5
96	14.0
97	13.5
98	13.5
99	14.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.471509672253102E-4
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0029658116067037225
7	0.007167378049533996
8	0.0017300567705771715
9	0.01087464255791365
10-11	0.0025950851558657574
12-13	3.707264508379653E-4
14-15	7.414529016759306E-4
16-17	4.943019344506204E-4
18-19	0.0028422361230910674
20-21	0.0
22-23	0.001977207737802482
24-25	0.003460113541154343
26-27	6.178774180632756E-4
28-29	0.005684472246182135
30-31	4.943019344506204E-4
32-33	0.0
34-35	0.004448717410055584
36-37	0.02076068124692606
38-39	0.010503916107075685
40-41	9.88603868901241E-4
42-43	9.88603868901241E-4
44-45	0.00506659482811886
46-47	0.0025950851558657574
48-49	3.707264508379653E-4
50-51	0.0017300567705771715
52-53	0.013964029648230028
54-55	0.01038034062346303
56-57	0.014829058033518614
58-59	0.014458331582680648
60-61	0.027433757362009432
62-63	0.011245369008751616
64-65	0.024344370271693058
66-67	0.02125498318137668
68-69	7.414529016759306E-4
70-71	2.471509672253102E-4
72-73	0.005437321278956825
74-75	0.011986821910427546
76	0.0029658116067037225
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	404611.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.386005632545135
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.8244300947123	43.1038432357535
2	8.810220270196208	9.75925819018101
3	4.805354384781959	7.984481550657269
4	2.7961289482000558	6.194656546973231
5	1.6612298964111163	4.600444419978922
6	0.9066209627022906	3.012846824808753
7	0.577028845590474	2.23715260244105
8	0.4198673189795997	1.860381895514059
9	0.3053623599134841	1.5221521247521952
>10	1.8413847470625508	17.876163202736187
>50	0.05100594089041305	1.7694180835755957
>100	0.0013662305595471553	0.07920132262820094
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02249073801750323	0.0
2	0.0	0.0	0.0	0.025209398656981644	0.0
3	0.0	0.0	0.0	0.025456549624206955	0.0
4	0.0	0.0	0.0	0.05363175988789232	0.0
5	0.0	0.0	0.0	0.059563383101299765	0.0
6	0.0	0.0	0.0	0.0627763456752288	0.0
7	0.0	0.0	0.0	0.06302349664245412	0.0
8	0.0	0.0	0.0	0.06351779857690473	0.0
9	0.0	0.0	0.0	0.0652478553474819	0.0
10	0.0	0.0	0.0	0.06821366695418563	0.0
11	0.0	0.0	0.0	0.06846081792141094	0.0
12	0.0	0.0	0.0	0.06895511985586156	0.0
13	0.0	0.0	0.0	0.06969657275753749	0.0
14	0.0	0.0	0.0	0.07216808242979059	0.0
15	0.0	0.0	0.0	0.07365098823314245	0.0
16	0.0	0.0	0.0	0.07439244113481838	0.0
17	0.0	0.0	0.0	0.0746395921020437	0.0
18	0.0	0.0	0.0	0.07612249790539555	0.0
19	0.0	0.0	0.0	0.07636964887262086	0.0
20	0.0	0.0	0.0	0.07809970564319803	0.0
21	0.0	0.0	0.0	0.08057121531545114	0.0
22	0.0	0.0	0.0	0.08501993272550672	0.0
23	0.0	2.471509672253102E-4	0.0	0.0869971404633092	0.0
24	0.0	2.471509672253102E-4	0.0	0.08996295207001292	0.0
25	0.0	2.471509672253102E-4	0.0	0.09317591464394195	0.0
26	0.0	2.471509672253102E-4	0.0	0.096388877217871	0.0
27	0.0	2.471509672253102E-4	0.0	0.11640810556312112	0.0
28	0.0	4.943019344506204E-4	0.0	0.13988744744952558	0.0
29	0.0	4.943019344506204E-4	0.0	0.16287248740147944	0.0
30	0.0	4.943019344506204E-4	0.0	0.19870937764914942	0.0
31	0.0	4.943019344506204E-4	0.0	0.23528772079849533	0.0
32	0.0	4.943019344506204E-4	0.0	0.27903344199737523	0.0
33	0.0	4.943019344506204E-4	0.0	0.3220377102945792	0.0
34	0.0	4.943019344506204E-4	0.0	0.38506120693703333	0.0
35	0.0	4.943019344506204E-4	0.0	0.4903475189750155	0.0
36	0.0	4.943019344506204E-4	0.0	0.6467940812286369	0.0
37	0.0	4.943019344506204E-4	0.0	0.9641359231459352	0.0
38	0.0	4.943019344506204E-4	0.0	1.4169164951027036	0.0
39	0.0	4.943019344506204E-4	0.0	1.945325263030417	0.0
40	0.0	4.943019344506204E-4	0.0	2.5209398656981645	0.0
41	0.0	4.943019344506204E-4	0.0	3.1988749687971905	0.0
42	0.0	4.943019344506204E-4	0.0	3.98728655424593	0.0
43	0.0	4.943019344506204E-4	0.0	4.733929626233592	0.0
44	0.0	4.943019344506204E-4	0.0	5.455116148597048	0.0
45	0.0	4.943019344506204E-4	0.0	6.242539130176886	0.0
46	0.0	4.943019344506204E-4	0.0	7.019581771133262	0.0
47	0.0	4.943019344506204E-4	0.0	7.8507504739119796	0.0
48	0.0	4.943019344506204E-4	0.0	8.566252524029252	0.0
49	0.0	4.943019344506204E-4	0.0	9.298066537983397	0.0
50	0.0	4.943019344506204E-4	0.0	10.018511607445175	0.0
51	0.0	4.943019344506204E-4	0.0	10.783196700040286	0.0
52	0.0	4.943019344506204E-4	0.0	11.604726515097218	0.0
53	0.0	4.943019344506204E-4	0.0	12.349886681281527	0.0
54	0.0	4.943019344506204E-4	0.0	13.036719219200664	0.0
55	0.0	4.943019344506204E-4	0.0	13.700566717167847	0.0
56	0.0	4.943019344506204E-4	0.0	14.36293130933168	0.0
57	0.0	4.943019344506204E-4	0.0	14.979820123526054	0.0
58	0.0	4.943019344506204E-4	0.0	15.592754522244823	0.0
59	0.0	4.943019344506204E-4	0.0	16.208654732570295	0.0
60	0.0	4.943019344506204E-4	0.0	16.84086690673264	0.0
61	0.0	4.943019344506204E-4	0.0	17.439713700319565	0.0
62	0.0	4.943019344506204E-4	0.0	17.994814772707613	0.0
63	0.0	4.943019344506204E-4	0.0	18.580315414064373	0.0
64	0.0	4.943019344506204E-4	0.0	19.107735578123187	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAA	30	5.8814103E-4	46.65612	10
CGACGAA	240	0.0	43.740112	22
CGACGAT	95	3.6379788E-12	40.51716	22
CGACCAT	35	0.0012562	39.99096	22
CTTACGC	35	0.0012562	39.99096	12
TCTCGTT	45	9.1968745E-5	38.884907	43
ACGCTTC	55	6.698836E-6	38.17319	15
TCTTACG	65	4.868325E-7	37.68379	11
GCCGTAT	85	2.561137E-9	37.073357	54
CGTACTC	40	0.0024158692	35.00507	37
CTCGTTG	50	1.7095573E-4	34.996418	44
GATATCC	40	0.0024188012	34.996418	26
CTTCCGA	80	6.2464096E-8	34.992092	18
TTACGCT	40	0.0024202683	34.992092	13
TGGTCGC	135	0.0	33.700253	49
AGATCTC	250	0.0	32.1967	40
TCGCCGT	120	6.548362E-11	32.08798	52
CGCTATG	55	2.9918485E-4	31.814922	25
GTCGAGG	45	0.004309958	31.10408	20
CGACGCA	45	0.004309958	31.10408	22
>>END_MODULE