FastQCFastQC Report
Wed 25 May 2016
SRR1779828_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779828_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404611
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT236755.85129914905922No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG29200.7216808242979058No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC16790.41496647397129593No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT7230.1786901493038993TruSeq Adapter, Index 12 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC6030.14903203323686207No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGCG150.002227225669.973313
TGGACGT200.006959285652.479985
TCTCGTA33400.051.43247638
ATCTCGT33400.051.32772437
TATGCCG34850.050.99919543
CGTATGC35050.050.80800641
CCGTCTT34800.050.7204747
TCGTATG35200.050.69088740
CTCGTAT34750.050.6425539
GCCGTCT34900.050.5751446
CGTCTTC35100.050.3867448
AATCTCG33500.050.33900536
CTGCTTG35150.050.2278554
ATGCCGT35300.050.05172344
TGCCGTC35350.050.00565345
GTATGCC35550.049.99499542
GTCTTCT35450.049.99424449
CTTCTGC35600.049.98036251
TCTTCTG35500.049.9238350
AATATCG506.2067556E-848.9813136
Produced by FastQC (version 0.11.5)