##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779828_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	404611
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.590310199178965	34.0	34.0	34.0	34.0	34.0
2	33.66107446411492	34.0	34.0	34.0	34.0	34.0
3	33.76795984291085	34.0	34.0	34.0	34.0	34.0
4	33.56277758143006	34.0	34.0	34.0	34.0	34.0
5	33.61786258900524	34.0	34.0	34.0	34.0	34.0
6	37.30595312534756	38.0	38.0	38.0	37.0	38.0
7	37.36076132383944	38.0	38.0	38.0	37.0	38.0
8	37.26553158465786	38.0	38.0	38.0	36.0	38.0
9	37.17798824055698	38.0	38.0	38.0	36.0	38.0
10-11	37.12205674091906	38.0	38.0	38.0	35.5	38.0
12-13	37.27430050097501	38.0	38.0	38.0	36.5	38.0
14-15	37.28964363302036	38.0	38.0	38.0	36.5	38.0
16-17	37.234352254387545	38.0	38.0	38.0	36.0	38.0
18-19	37.11778720796024	38.0	38.0	38.0	36.0	38.0
20-21	37.11056545669791	38.0	38.0	38.0	36.0	38.0
22-23	37.091508881370004	38.0	38.0	38.0	36.0	38.0
24-25	37.291832154835134	38.0	38.0	38.0	36.5	38.0
26-27	37.22698097679005	38.0	38.0	38.0	36.5	38.0
28-29	36.75182953503489	38.0	37.0	38.0	34.5	38.0
30-31	37.02285281418449	38.0	38.0	38.0	36.0	38.0
32-33	37.09760362422179	38.0	38.0	38.0	36.0	38.0
34-35	37.14825845070945	38.0	38.0	38.0	36.0	38.0
36-37	37.12698493120553	38.0	38.0	38.0	36.0	38.0
38-39	36.871236817585284	38.0	38.0	38.0	35.5	38.0
40-41	36.98239543660454	38.0	38.0	38.0	35.5	38.0
42-43	37.21712212470743	38.0	38.0	38.0	36.5	38.0
44-45	36.99056501182618	38.0	38.0	38.0	35.5	38.0
46-47	37.12245588973113	38.0	38.0	38.0	36.0	38.0
48-49	36.79060999330221	38.0	37.0	38.0	35.0	38.0
50-51	36.97565562972831	38.0	37.5	38.0	35.5	38.0
52-53	36.90643976560202	38.0	38.0	38.0	35.5	38.0
54-55	36.904399534367585	38.0	37.5	38.0	35.5	38.0
56-57	36.781554381863074	38.0	37.0	38.0	35.0	38.0
58-59	36.89318258772006	38.0	37.5	38.0	35.5	38.0
60-61	36.60228466354103	38.0	37.0	38.0	34.0	38.0
62-63	36.41731070089543	38.0	37.0	38.0	34.0	38.0
64-65	36.74766627699198	38.0	37.0	38.0	34.5	38.0
66-67	36.55932611817276	38.0	37.0	38.0	34.5	38.0
68-69	36.67483212270551	38.0	37.0	38.0	34.0	38.0
70-71	36.220247348688005	38.0	37.0	38.0	32.5	38.0
72-73	35.44981846761458	38.0	37.0	38.0	28.5	38.0
74-75	35.337822006816424	38.0	37.0	38.0	26.0	38.0
76	35.075605952383896	38.0	37.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	2.0
21	2.0
22	13.0
23	28.0
24	77.0
25	157.0
26	285.0
27	597.0
28	1029.0
29	1738.0
30	2979.0
31	4600.0
32	7518.0
33	12300.0
34	20360.0
35	37666.0
36	61701.0
37	253556.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.87893743864655	18.32109767345253	11.38398542106083	30.41597946684008
2	19.83312366692947	19.697437785922776	36.93918356149487	23.53025498565289
3	18.055861061612262	21.556013059457108	34.56727572903356	25.820850149897062
4	12.873352429864735	22.93338539980376	36.2306017384599	27.9626604318716
5	12.542170133782818	33.19064484158859	39.961839890660414	14.305345133968181
6	26.331958350119006	41.57054553632996	18.63320572105059	13.46429039250045
7	25.434058886189455	28.845977988734862	22.06489690097402	23.655066224101667
8	22.658553524249218	39.127211074340536	19.21326904112839	19.00096636028185
9	22.644465919117373	17.323799896690893	20.39267345672757	39.63906072746416
10-11	23.559913101719925	27.367273751825827	27.993677878258378	21.079135268195873
12-13	23.366146743415282	21.974192496002335	28.088336698705668	26.571324061876716
14-15	21.30243616708394	22.5353487670874	22.667203808102105	33.49501125772656
16-17	25.059501595359496	28.395792501933958	23.400130001408762	23.144575901297788
18-19	25.08545244691815	25.38203360758852	24.70669358964536	24.825820355847963
20-21	21.31825382898636	24.8750033983258	24.439646969558414	29.367095803129427
22-23	21.566269824596958	28.30904251243787	24.45002731018188	25.67466035278329
24-25	25.076030849803075	28.336985038512825	24.30046057208812	22.286523539595976
26-27	24.58423028538522	28.983764652962968	24.98164904068352	21.45035602096829
28-29	21.18615163700443	25.45259520873135	28.559900744171564	24.801352410092655
30-31	20.957784143288244	28.244783260959295	25.979644646339324	24.817787949413137
32-33	20.627590451075232	25.495352326061326	31.804869368356275	22.07218785450717
34-35	25.13784845196992	27.8708438475474	26.07393274033578	20.917374960146905
36-37	21.59481576131148	32.15520586439815	24.73684600764685	21.513132366643518
38-39	20.75685040693407	25.836914962766706	28.956330895600964	24.449903734698267
40-41	20.924295187229216	23.725257098793655	29.788735353215806	25.561712360761323
42-43	24.285761950720385	23.62524854211283	29.47187349777996	22.617116009386827
44-45	20.507969382938178	27.35479262798095	30.093719646771838	22.04351834230903
46-47	23.686083670488443	23.485149934134267	27.35244469379231	25.47632170158498
48-49	23.101942359451424	23.510853634725702	26.950206494633118	26.43699751118976
50-51	20.074405037789603	23.58750934697838	30.442042554243375	25.89604306098865
52-53	19.01095138199543	23.720824437936606	34.171179779933965	23.097044400134003
54-55	18.575692531393273	24.093687714213647	31.08935522001256	26.241264534380516
56-57	21.90705110371714	23.84691759184042	27.536979129959555	26.709052174482885
58-59	18.856224502410683	23.856100877735194	33.24329336135492	24.044381258499197
60-61	22.21389833567597	27.44904607023959	26.79088213005784	23.546173464026595
62-63	18.529982570429066	31.96237190501502	25.337033511749507	24.17061201280641
64-65	18.52174117245735	32.605393486732574	24.41319660308471	24.459668737725362
66-67	18.582683589872286	32.135258725023455	24.14128165220068	25.140776032903577
68-69	18.76469912142046	32.81603960714184	23.84103259358312	24.57822867785458
70-71	19.20981532612699	31.925023050245827	23.687371925912927	25.177789697714253
72-73	19.2618092595225	30.36866274003251	23.861162786385744	26.508365214059243
74-75	19.27825355058071	29.731440019874107	24.347633708362327	26.64267272118285
76	19.37452571578294	29.8484515161028	24.539434492543695	26.237588275570563
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.5
11	5.0
12	8.0
13	10.0
14	21.0
15	36.5
16	54.0
17	65.0
18	104.5
19	170.0
20	261.5
21	327.0
22	406.5
23	610.0
24	978.5
25	1223.0
26	1427.0
27	1876.0
28	2386.5
29	2652.0
30	3151.0
31	3837.0
32	5077.5
33	6131.0
34	6948.0
35	8601.0
36	10332.0
37	11227.0
38	16301.0
39	24844.5
40	28313.5
41	27080.5
42	25848.0
43	25513.0
44	25467.0
45	25049.0
46	24342.0
47	24710.5
48	23971.0
49	21906.5
50	20950.0
51	19660.5
52	16350.5
53	13415.0
54	12500.0
55	11564.5
56	9702.5
57	7661.5
58	6547.0
59	5750.5
60	4512.0
61	3734.5
62	3399.0
63	3043.5
64	2555.5
65	2194.5
66	2054.0
67	2142.0
68	1979.0
69	1620.5
70	1403.0
71	1381.0
72	1280.5
73	1118.5
74	1020.0
75	983.0
76	887.0
77	744.5
78	660.5
79	623.0
80	554.0
81	471.0
82	406.0
83	355.0
84	316.5
85	213.0
86	114.5
87	81.0
88	67.0
89	42.0
90	30.5
91	19.0
92	8.0
93	6.0
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04745298570725956
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0013593303197392062
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	3.707264508379653E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.016929841254933752
52-53	0.034971861862381395
54-55	0.05560896762569481
56-57	0.05622684504375808
58-59	0.039791305723274946
60-61	0.037690522501859815
62-63	0.031141021870389092
64-65	0.01668269028770844
66-67	0.038679126370761055
68-69	0.009268161270949133
70-71	0.014829058033518614
72-73	0.02681587994394616
74-75	0.01594123738603251
76	0.013346152230166752
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	404611.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.74802217438478
#Duplication Level	Percentage of deduplicated	Percentage of total
1	39.15211699134081	8.906332254931279
2	15.497441357655827	7.05072279300365
3	12.358622787670711	8.434026756563712
4	9.35561325930835	8.512867915108584
5	6.494931606566638	7.387342410364522
6	4.273095685618366	5.832268524582871
7	2.623830684151628	4.17808710094387
8	1.7503069284340675	3.185281665599798
9	1.1440553666300888	2.342249716394265
>10	6.943644680088222	30.231753461967177
>50	0.3628817592160016	5.379487952626103
>100	0.03802653165437142	1.2439108180449865
>500	0.002172944665964081	0.32772218254076135
>1k	0.002172944665964081	1.1366472982692017
>5k	0.0	0.0
>10k+	0.0010864723329820406	5.85129914905922
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT	23675	5.85129914905922	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG	2920	0.7216808242979058	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC	1679	0.41496647397129593	No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT	723	0.1786901493038993	TruSeq Adapter, Index 12 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC	603	0.14903203323686207	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.033859682509867504	0.0
2	0.0	0.0	0.0	0.037814097985472465	0.0
3	0.0	0.0	0.0	0.038061248952697774	0.0
4	0.0	0.0	0.0	0.074886743069269	0.0
5	0.0	0.0	0.0	0.08254842305325362	0.0
6	0.0	0.0	0.0	0.08650283852885858	0.0
7	0.0	0.0	0.0	0.08773859336498513	0.0
8	0.0	0.0	0.0	0.08773859336498513	0.0
9	0.0	0.0	0.0	0.08971580110278761	0.0
10	0.0	0.0	0.0	0.09342306561116727	0.0
11	0.0	0.0	0.0	0.09490597141451913	0.0
12	0.0	0.0	0.0	0.09564742431619506	0.0
13	0.0	0.0	0.0	0.0966360281850963	0.0
14	0.0	0.0	0.0	0.09861323592289878	0.0
15	0.0	0.0	0.0	0.10083759462792657	0.0
16	0.0	0.0	0.0	0.10182619849682782	0.0
17	0.0	0.0	0.0	0.10232050043127844	0.0
18	0.0	0.0	0.0	0.10503916107075685	0.0
19	0.0	0.0	0.0	0.10553346300520747	0.0
20	0.0	0.0	0.0	0.10825212364468588	0.0
21	0.0	0.0	0.0	0.11097078428416429	0.0
22	0.0	0.0	0.0	0.1134422939564174	0.0
23	0.0	0.0	0.0	0.11616095459589582	0.0
24	0.0	0.0	0.0	0.11863246426814891	0.0
25	0.0	0.0	0.0	0.12184542684207794	0.0
26	0.0	0.0	0.0	0.12604699328490823	0.0
27	0.0	0.0	0.0	0.14606622163015834	0.0
28	0.0	0.0	0.0	0.17078131835268937	0.0
29	0.0	0.0	0.0	0.19475496217354446	0.0
30	0.0	0.0	0.0	0.23331051306069286	0.0
31	0.0	0.0	0.0	0.27359612071841843	0.0
32	0.0	0.0	0.0	0.3225320122290299	0.0
33	0.0	0.0	0.0	0.3694906960018388	0.0
34	0.0	0.0	0.0	0.4369629100543485	0.0
35	0.0	0.0	0.0	0.552876713683019	0.0
36	0.0	0.0	0.0	0.7290953533146652	0.0
37	0.0	0.0	0.0	1.0684336313150162	0.0
38	0.0	0.0	0.0	1.5491422625682445	0.0
39	0.0	0.0	0.0	2.1059733917268684	0.0
40	0.0	0.0	0.0	2.7137176201339064	0.0
41	0.0	0.0	0.0	3.439105708940192	0.0
42	0.0	0.0	0.0	4.277194638801219	0.0
43	0.0	0.0	0.0	5.062393261676029	0.0
44	0.0	0.0	0.0	5.819169523319929	0.0
45	0.0	0.0	0.0	6.649843924164197	0.0
46	0.0	0.0	0.0	7.487685703057999	0.0
47	0.0	0.0	0.0	8.36927320315068	0.0
48	0.0	0.0	0.0	9.130498182204636	0.0
49	0.0	0.0	0.0	9.91767401281725	0.0
50	0.0	0.0	0.0	10.675191727362826	0.0
51	0.0	0.0	0.0	11.477690917943407	0.0
52	0.0	0.0	0.0	12.319487112312814	0.0
53	0.0	0.0	0.0	13.072061807513883	0.0
54	0.0	0.0	0.0	13.768038931220357	0.0
55	0.0	0.0	0.0	14.436582297564822	0.0
56	0.0	0.0	0.0	15.10438421100761	0.0
57	0.0	0.0	0.0	15.73363057356325	0.0
58	0.0	0.0	0.0	16.35916967161051	0.0
59	0.0	0.0	0.0	16.99484195931401	0.0
60	0.0	0.0	0.0	17.63496296442756	0.0
61	0.0	0.0	0.0	18.25061602378581	0.0
62	0.0	0.0	0.0	18.821040456141827	0.0
63	0.0	0.0	0.0	19.43002043938499	0.0
64	0.0	0.0	0.0	19.98808732337974	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAAGCG	15	0.0022272256	69.97331	3
TGGACGT	20	0.0069592856	52.47998	5
TCTCGTA	3340	0.0	51.432476	38
ATCTCGT	3340	0.0	51.327724	37
TATGCCG	3485	0.0	50.999195	43
CGTATGC	3505	0.0	50.808006	41
CCGTCTT	3480	0.0	50.72047	47
TCGTATG	3520	0.0	50.690887	40
CTCGTAT	3475	0.0	50.64255	39
GCCGTCT	3490	0.0	50.57514	46
CGTCTTC	3510	0.0	50.38674	48
AATCTCG	3350	0.0	50.339005	36
CTGCTTG	3515	0.0	50.22785	54
ATGCCGT	3530	0.0	50.051723	44
TGCCGTC	3535	0.0	50.005653	45
GTATGCC	3555	0.0	49.994995	42
GTCTTCT	3545	0.0	49.994244	49
CTTCTGC	3560	0.0	49.980362	51
TCTTCTG	3550	0.0	49.92383	50
AATATCG	50	6.2067556E-8	48.98131	36
>>END_MODULE