FastQCFastQC Report
Wed 25 May 2016
SRR1779827_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779827_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221310
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA150.002222410670.000958
GCGTAGA352.9646617E-760.00077436
GTCTTGA200.00693809652.51254723
ACGTTGC407.456001E-752.5006815
AATCTCG200.00694430252.5006841
GAGTCTA200.00695051252.48881568
ATCTCGG1700.051.47125642
GATCTCG1700.049.41240341
GTAGATC2150.047.2099138
AGATCTC2100.046.66726740
TGGTCGC1350.046.65672749
TCGGTGG1400.045.0005845
CGGTGGT1500.044.3238946
TAGATCT2150.043.95405639
CGATATG404.5762652E-543.76045625
TCTCGGT1900.042.3689743
GACGAAT757.4942363E-1042.01003623
TGTAGAT2750.042.0005437
TAGTGTA855.638867E-1141.17700234
CGACGAA2400.040.8338622
Produced by FastQC (version 0.11.5)