FastQCFastQC Report
Wed 25 May 2016
SRR1779827_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779827_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221310
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT3189814.413266458813428TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG41211.8620938954407844TruSeq Adapter, Index 5 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC19620.8865392435949573RNA PCR Primer, Index 5 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT8790.3971804256472821TruSeq Adapter, Index 5 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC7850.3547060684108264RNA PCR Primer, Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGT5050.22818670642989472No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGAACTCGTATGCCGT4740.2141792056391487No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATATCGTATGCCGT3680.1662825900320817No Hit
CTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC3530.1595047670688175TruSeq Adapter, Index 5 (95% over 24bp)
CTTTACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC2380.10754145768379197RNA PCR Primer, Index 5 (95% over 24bp)
CTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG2270.10257105417739822TruSeq Adapter, Index 5 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCT207.881129E-570.05971554
CGTCTTA207.8899655E-570.0438848
TCGTGGC150.00222497369.9805732
GACACCA308.371608E-658.31714226
GGCGTAA252.3820848E-456.0224367
GCGTAAA200.006933665452.52102768
CTTCCGA200.00695228752.48542816
GCGAAGC200.00695228752.48542837
GACCGTG200.00695228752.48542811
CGATTCA200.00695228752.48542834
CCCCGGT200.00695228752.4854287
ACGCTTA200.00695228752.48542831
TGTCGGA200.00695228752.48542835
AAGCTTG200.00695228752.4854289
TCTCGTA46750.052.0176438
ATCTCGT46550.052.01563337
CTCGTAT48300.052.01454539
TCGTATG49200.051.8453640
CGTATGC49300.051.74019641
GCCGTCT48950.051.70462446
Produced by FastQC (version 0.11.5)