##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779827_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	221310
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.583674483755814	34.0	34.0	34.0	34.0	34.0
2	33.657792237133435	34.0	34.0	34.0	34.0	34.0
3	33.768094528037594	34.0	34.0	34.0	34.0	34.0
4	33.57148795806787	34.0	34.0	34.0	34.0	34.0
5	33.62909041615833	34.0	34.0	34.0	34.0	34.0
6	37.321042881026614	38.0	38.0	38.0	37.0	38.0
7	37.368465049026256	38.0	38.0	38.0	37.0	38.0
8	37.279779494826265	38.0	38.0	38.0	36.0	38.0
9	37.190958384167004	38.0	38.0	38.0	36.0	38.0
10-11	37.13965478288374	38.0	38.0	38.0	35.5	38.0
12-13	37.29232298585694	38.0	38.0	38.0	36.5	38.0
14-15	37.31511228593376	38.0	38.0	38.0	36.5	38.0
16-17	37.226316027292036	38.0	38.0	38.0	36.0	38.0
18-19	37.0863404274547	38.0	38.0	38.0	35.5	38.0
20-21	37.11512358230536	38.0	38.0	38.0	36.0	38.0
22-23	37.09836428539153	38.0	38.0	38.0	36.0	38.0
24-25	37.24428855451629	38.0	38.0	38.0	36.0	38.0
26-27	37.15650219149609	38.0	38.0	38.0	36.0	38.0
28-29	36.72168903348245	38.0	37.0	38.0	34.0	38.0
30-31	36.906843341918574	38.0	37.0	38.0	35.5	38.0
32-33	36.91264063982649	38.0	38.0	38.0	35.5	38.0
34-35	36.9769870317654	38.0	38.0	38.0	36.0	38.0
36-37	37.02032895034115	38.0	38.0	38.0	36.0	38.0
38-39	36.08577786814875	38.0	37.0	38.0	34.0	38.0
40-41	36.693622068591566	38.0	37.0	38.0	35.0	38.0
42-43	37.162087117617816	38.0	38.0	38.0	36.0	38.0
44-45	36.9538249514256	38.0	38.0	38.0	35.5	38.0
46-47	37.10116352627536	38.0	38.0	38.0	36.0	38.0
48-49	36.742824544756225	38.0	37.0	38.0	34.5	38.0
50-51	36.89494148479508	38.0	37.5	38.0	35.0	38.0
52-53	36.816723148524694	38.0	37.5	38.0	35.0	38.0
54-55	36.8141633907189	38.0	37.0	38.0	35.0	38.0
56-57	36.70273824047716	38.0	37.0	38.0	34.5	38.0
58-59	36.767827933667704	38.0	37.0	38.0	34.5	38.0
60-61	36.44736116759297	38.0	37.0	38.0	34.0	38.0
62-63	36.29550404410104	38.0	37.0	38.0	34.0	38.0
64-65	36.68863133161628	38.0	37.0	38.0	34.5	38.0
66-67	36.420891509647106	38.0	37.0	38.0	34.0	38.0
68-69	36.44618408567168	38.0	37.0	38.0	34.0	38.0
70-71	35.30161312186526	38.0	36.5	38.0	28.5	38.0
72-73	33.22303782025214	38.0	34.0	38.0	20.0	38.0
74-75	32.91060277438886	38.0	34.0	38.0	11.0	38.0
76	32.699511996746644	38.0	32.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	0.0
21	1.0
22	3.0
23	18.0
24	68.0
25	120.0
26	232.0
27	412.0
28	767.0
29	1298.0
30	2081.0
31	3294.0
32	5136.0
33	8400.0
34	14077.0
35	28853.0
36	37169.0
37	119380.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.13037483102991	15.57418182311375	11.32359523131383	38.9718481145425
2	17.143825403280466	15.981202837648548	43.23256969861281	23.64240206045818
3	16.020966065699696	17.830192942027022	41.933486963987164	24.215354028286114
4	11.761330260720257	29.4740409380507	32.34693416474628	26.417694636482764
5	11.523202747277574	30.210564366725407	45.222538520627175	13.043694365369843
6	24.698838732998958	46.26903438615517	16.37612398897474	12.656002891871132
7	23.857936830689983	25.94821743256066	18.392300393113732	31.801545343635624
8	20.746012380823277	44.53843025620171	15.848809362432787	18.866748000542223
9	20.146852830870724	15.816275812209119	16.866386516650852	47.1704848402693
10-11	20.74962721973702	29.486015091952467	29.17468709050653	20.589670597803984
12-13	20.53951470787583	18.91938909222358	30.64050427002847	29.90059192987212
14-15	18.918485382495142	19.26392842618951	20.214631060503365	41.60295513081198
16-17	28.47386019610501	29.52510053770729	19.66088292440468	22.34015634178302
18-19	28.546834756676155	23.305996114048167	20.705571370475802	27.441597758799873
20-21	19.06985676200804	21.375672134110523	20.612489268446975	38.94198183543446
22-23	19.223261488409925	30.276987031765394	20.533188739776783	29.966562740047898
24-25	28.24863990456739	30.248567607135758	20.440291358649485	21.06250112964737
26-27	28.077809407618272	31.56816230626723	20.854231620803397	19.499796665311102
28-29	18.923907640865753	31.565225249649814	21.422890967421264	28.08797614206317
30-31	18.465274953684876	31.60363291310831	20.90664678505264	29.02444534815417
32-33	18.365866883557004	40.67823415119064	21.25841579684605	19.697483168406308
34-35	28.060638922778004	23.73593601735123	29.723012968234602	18.48041209163617
36-37	28.965478288373774	29.008404500474448	22.99534589489856	19.03077131625322
38-39	19.370792101576974	22.85459310469477	30.60141882427364	27.17319596945461
40-41	18.327685147530612	20.379558085942794	32.19578871266549	29.0969680538611
42-43	27.607553240039945	19.951515753991025	31.14920766891541	21.29172333705362
44-45	18.48718991459943	29.18914644616149	32.5961321223623	19.727531516876777
46-47	26.884912566083774	20.110930369165423	24.772717003298542	28.23144006145226
48-49	25.968550901450456	19.528941304053138	23.70679137860919	30.795716415887213
50-51	18.593311144101214	19.6939603260145	32.66336313740376	29.049365392480514
52-53	16.281187850298316	19.853552702947024	41.64459410594829	22.220665340806363
54-55	15.948662656784363	20.046473044052195	34.3674489779773	29.63741532118615
56-57	24.896132371889056	19.992088428761953	25.54284680937634	29.568932389972645
58-59	16.48050627189513	20.41134591479263	39.953667081026104	23.154480732286135
60-61	25.16317416695294	29.13630693015603	24.532851796271853	21.167667106619177
62-63	16.312758764079476	40.329907251722084	21.499114100269388	21.858219883929056
64-65	15.503574688852936	41.11547465179548	20.899953904138684	22.4809967552129
66-67	15.866554867230636	41.114543946444755	20.444154147615937	22.57474703870867
68-69	16.12122608896059	41.48285725905942	19.890731125360954	22.505185526619037
70-71	16.356993241640172	40.39967191111774	19.296687703219657	23.946647144022435
72-73	16.76297051226716	36.516971999195526	19.790609753617325	26.929447734919993
74-75	17.42076744743062	35.598723783097356	19.65776237238961	27.322746397082415
76	17.438166296846966	36.28631727366808	19.381866331644666	26.893650097840283
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	1.0
15	9.5
16	21.5
17	25.0
18	32.0
19	75.0
20	118.5
21	126.0
22	152.0
23	260.5
24	444.5
25	546.0
26	666.0
27	833.0
28	1046.0
29	1212.0
30	1459.0
31	1885.0
32	2459.0
33	2854.0
34	3186.0
35	3908.0
36	4695.5
37	5093.0
38	5847.0
39	12371.5
40	20671.5
41	22089.5
42	20978.0
43	18645.5
44	14616.0
45	12124.5
46	11330.0
47	11332.0
48	10695.5
49	9633.5
50	9210.0
51	8752.0
52	7567.5
53	6362.5
54	5884.0
55	5437.5
56	4701.5
57	3933.5
58	3455.0
59	3112.5
60	2588.0
61	2274.0
62	2142.0
63	1998.0
64	1780.0
65	1646.0
66	1468.0
67	1350.0
68	1309.0
69	1207.0
70	1106.0
71	1066.0
72	1016.5
73	948.0
74	891.5
75	854.0
76	766.0
77	638.0
78	563.0
79	528.0
80	480.0
81	380.0
82	303.5
83	279.0
84	239.5
85	184.5
86	137.5
87	106.0
88	91.5
89	70.0
90	45.0
91	18.0
92	9.0
93	10.0
94	5.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.052867019113460756
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	9.037097284352266E-4
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	4.518548642176133E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.014233428222854819
52-53	0.03162984049523293
54-55	0.04812254303917581
56-57	0.05196330938502553
58-59	0.03840766345849713
60-61	0.03343725995210338
62-63	0.029822421038362478
64-65	0.014459355654963625
66-67	0.03637431656951787
68-69	0.00948895214856988
70-71	0.01287786363020198
72-73	0.021914960914554246
74-75	0.014007500790746011
76	0.014007500790746011
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	221310.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.57385567755637
#Duplication Level	Percentage of deduplicated	Percentage of total
1	35.62110164280067	7.32863404274547
2	12.305631204427655	5.063485608422575
3	11.354651673548274	7.008268944015182
4	10.155495036457877	8.357507568568975
5	8.569797065799877	8.815688400885636
6	6.419660897830097	7.924630608648502
7	4.5506456997276645	6.553702950612263
8	2.819994728981815	4.641453165243324
9	1.8909777738733198	3.501423342822285
>10	6.18905385223579	20.34521711626226
>50	0.07906527277519108	1.0202882834033709
>100	0.03074760607924097	1.2977271700329853
>500	0.0065887727312659225	0.9800732004880033
>1k	0.004392515154177282	2.7486331390357415
>5k	0.0	0.0
>10k+	0.002196257577088641	14.413266458813428
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT	31898	14.413266458813428	TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG	4121	1.8620938954407844	TruSeq Adapter, Index 5 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC	1962	0.8865392435949573	RNA PCR Primer, Index 5 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT	879	0.3971804256472821	TruSeq Adapter, Index 5 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC	785	0.3547060684108264	RNA PCR Primer, Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGT	505	0.22818670642989472	No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGAACTCGTATGCCGT	474	0.2141792056391487	No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATATCGTATGCCGT	368	0.1662825900320817	No Hit
CTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC	353	0.1595047670688175	TruSeq Adapter, Index 5 (95% over 24bp)
CTTTACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC	238	0.10754145768379197	RNA PCR Primer, Index 5 (95% over 24bp)
CTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG	227	0.10257105417739822	TruSeq Adapter, Index 5 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.003162984049523293	0.0
2	0.0	0.0	0.0	0.003162984049523293	0.0
3	0.0	0.0	0.0	0.003162984049523293	0.0
4	0.0	0.0	0.0	0.005422258370611359	0.0
5	0.0	0.0	0.0	0.010844516741222719	0.0
6	0.0	0.0	0.0	0.010844516741222719	0.0
7	0.0	0.0	0.0	0.012651936198093173	0.0
8	0.0	0.0	0.0	0.012651936198093173	0.0
9	0.0	0.0	0.0	0.016718629976051693	0.0
10	0.0	0.0	0.0	0.018526049432922145	0.0
11	0.0	0.0	0.0	0.022592743210880666	0.0
12	0.0	0.0	0.0	0.023044598075098278	0.0
13	0.0	0.0	0.0	0.023044598075098278	0.0
14	0.0	0.0	0.0	0.023044598075098278	0.0
15	0.0	0.0	0.0	0.025303872396186346	0.0
16	0.0	0.0	0.0	0.025303872396186346	0.0
17	0.0	0.0	0.0	0.025303872396186346	0.0
18	0.0	0.0	0.0	0.025303872396186346	0.0
19	0.0	0.0	0.0	0.02756314671727441	0.0
20	0.0	0.0	0.0	0.02756314671727441	0.0
21	0.0	0.0	0.0	0.028015001581492026	0.0
22	0.0	0.0	0.0	0.030726130766797706	0.0
23	0.0	0.0	0.0	0.03298540508788577	0.0
24	0.0	0.0	0.0	0.038859518322714746	0.0
25	0.0	0.0	0.0	0.04292621210067327	0.0
26	0.0	0.0	0.0	0.046089196150196556	0.0
27	0.0	0.0	0.0	0.07184492341060052	0.0
28	0.0	0.0	0.0	0.11251186119018572	0.0
29	0.0	0.0	0.0	0.15001581492024763	0.0
30	0.0	0.0	0.0	0.1965568659346618	0.0
31	0.0	0.0	0.0	0.23993493289955267	0.0
32	0.0	0.0	0.0	0.28421670959287876	0.0
33	0.0	0.0	0.0	0.3474763905833446	0.0
34	0.0	0.0	0.0	0.4292621210067326	0.0
35	0.0	0.0	0.0	0.5706926935068456	0.0
36	0.0	0.0	0.0	0.793457141566129	0.0
37	0.0	0.0	0.0	1.1612670010392663	0.0
38	0.0	0.0	0.0	1.660566625999729	0.0
39	0.0	0.0	0.0	2.3211784374858797	0.0
40	0.0	0.0	0.0	3.01206452487461	0.0
41	0.0	0.0	0.0	3.769825132167548	0.0
42	0.0	0.0	0.0	4.480140978717636	0.0
43	0.0	0.0	0.0	5.300257557272604	0.0
44	0.0	0.0	0.0	6.1542632506438935	0.0
45	0.0	0.0	0.0	7.076047173647824	0.0
46	0.0	0.0	0.0	8.067868600605486	0.0
47	0.0	0.0	0.0	9.082734625638246	0.0
48	0.0	0.0	0.0	9.934481044688447	0.0
49	0.0	0.0	0.0	10.863946500384076	0.0
50	0.0	0.0	0.0	11.654240657900683	0.0
51	0.0	0.0	0.0	12.532194659075506	0.0
52	0.0	0.0	0.0	13.281370023948307	0.0
53	0.0	0.0	0.0	14.025123130450499	0.0
54	0.0	0.0	0.0	14.793728254484659	0.0
55	0.0	0.0	0.0	15.534318376937328	0.0
56	0.0	0.0	0.0	16.315123582305365	0.0
57	0.0	0.0	0.0	16.947268537345806	0.0
58	0.0	0.0	0.0	17.605621074510868	0.0
59	0.0	0.0	0.0	18.33717409967918	0.0
60	0.0	0.0	0.0	19.04161583299444	0.0
61	0.0	0.0	0.0	19.73521304956848	0.0
62	0.0	0.0	0.0	20.33708372870634	0.0
63	0.0	0.0	0.0	20.969680538610998	0.0
64	0.0	0.0	0.0	21.6316479146898	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATTCT	20	7.881129E-5	70.059715	54
CGTCTTA	20	7.8899655E-5	70.04388	48
TCGTGGC	15	0.002224973	69.98057	32
GACACCA	30	8.371608E-6	58.317142	26
GGCGTAA	25	2.3820848E-4	56.02243	67
GCGTAAA	20	0.0069336654	52.521027	68
CTTCCGA	20	0.006952287	52.485428	16
GCGAAGC	20	0.006952287	52.485428	37
GACCGTG	20	0.006952287	52.485428	11
CGATTCA	20	0.006952287	52.485428	34
CCCCGGT	20	0.006952287	52.485428	7
ACGCTTA	20	0.006952287	52.485428	31
TGTCGGA	20	0.006952287	52.485428	35
AAGCTTG	20	0.006952287	52.485428	9
TCTCGTA	4675	0.0	52.01764	38
ATCTCGT	4655	0.0	52.015633	37
CTCGTAT	4830	0.0	52.014545	39
TCGTATG	4920	0.0	51.84536	40
CGTATGC	4930	0.0	51.740196	41
GCCGTCT	4895	0.0	51.704624	46
>>END_MODULE