FastQCFastQC Report
Wed 25 May 2016
SRR1779826_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779826_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347840
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATC150.002223342270.00201438
ACGAAGT207.925369E-569.9919424
AGATCTC1550.054.18731740
GGTCCCC200.0069471952.5015150
GTGTCGA200.0069471952.5015136
AGCGAGA200.0069471952.5015110
TGTGTCG200.0069471952.5015135
GGTAGCC200.0069471952.5015150
ACGTGGC200.00695114252.4939615
CTCGGTG1100.050.90323344
CGGTGGT1100.050.90323346
GTAGATC1600.050.31394638
TAGATCC352.0858637E-549.9942539
GATCTCG1550.049.67170741
TCGGTGG1200.049.5776345
TAGATCT1750.045.9947139
ATCTCGG1550.045.15609742
ACGCTTC551.3102726E-744.5403315
TCTCGGT1750.043.99493843
CTTCCGA404.591282E-543.74496518