##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779826_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	347840
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.14784958601656	34.0	27.0	34.0	12.0	34.0
2	30.122642594296227	34.0	31.0	34.0	12.0	34.0
3	29.709613615455382	34.0	31.0	34.0	11.0	34.0
4	29.82651218951242	34.0	31.0	34.0	12.0	34.0
5	30.48413063477461	34.0	32.0	34.0	21.0	34.0
6	33.15489017939282	37.0	34.0	38.0	21.0	38.0
7	33.31154553817847	37.0	34.0	38.0	21.0	38.0
8	32.4303760349586	37.0	31.0	38.0	11.0	38.0
9	33.36305485280589	37.0	34.0	38.0	21.0	38.0
10-11	31.888852633394663	37.0	29.5	38.0	11.5	38.0
12-13	32.87209636614536	37.0	33.5	38.0	11.0	38.0
14-15	31.58648660303588	37.0	28.5	38.0	11.0	38.0
16-17	32.2808101425943	37.0	30.5	38.0	16.0	38.0
18-19	33.299784383624655	37.5	34.0	38.0	21.0	38.0
20-21	31.813856945722172	37.0	29.5	38.0	16.0	38.0
22-23	32.773658866145354	37.0	32.5	38.0	16.0	38.0
24-25	33.121535763569455	37.5	33.5	38.0	16.0	38.0
26-27	31.998105450781967	37.0	29.0	38.0	11.0	38.0
28-29	33.35605738270469	37.5	34.0	38.0	21.0	38.0
30-31	33.83900931462742	38.0	34.0	38.0	21.0	38.0
32-33	33.93482060717571	38.0	34.5	38.0	21.0	38.0
34-35	33.83516271849126	38.0	34.5	38.0	21.0	38.0
36-37	33.26926029208832	38.0	34.0	38.0	16.0	38.0
38-39	33.202630519779206	37.5	33.5	38.0	16.0	38.0
40-41	33.1819370975161	37.5	34.0	38.0	16.0	38.0
42-43	33.6558115800368	38.0	34.5	38.0	21.0	38.0
44-45	33.711837339006436	38.0	34.0	38.0	21.0	38.0
46-47	33.609761672033116	38.0	34.0	38.0	21.0	38.0
48-49	33.30316524839006	37.5	34.0	38.0	16.0	38.0
50-51	32.59021532888684	37.5	32.5	38.0	11.0	38.0
52-53	33.18691783578657	37.0	34.0	38.0	21.0	38.0
54-55	33.36614966651334	37.5	34.0	38.0	16.0	38.0
56-57	33.456048758049675	38.0	34.0	38.0	21.0	38.0
58-59	33.79943939742411	38.0	34.0	38.0	21.0	38.0
60-61	33.617269434222635	38.0	34.0	38.0	21.0	38.0
62-63	33.6682540823367	38.0	34.0	38.0	21.0	38.0
64-65	33.68839696412144	38.0	34.5	38.0	21.0	38.0
66-67	33.49201356945722	38.0	34.0	38.0	21.0	38.0
68-69	32.78644635464582	37.0	32.5	38.0	11.0	38.0
70-71	32.83696958371665	37.0	32.0	38.0	16.0	38.0
72-73	33.067260809567614	37.0	33.0	38.0	19.5	38.0
74-75	33.2299361775529	37.0	33.5	38.0	20.0	38.0
76	32.21794790708372	37.0	31.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	54.0
13	693.0
14	2194.0
15	3675.0
16	4876.0
17	6087.0
18	6189.0
19	5605.0
20	4770.0
21	3875.0
22	3356.0
23	3074.0
24	3265.0
25	3673.0
26	4194.0
27	5135.0
28	6042.0
29	7313.0
30	8943.0
31	11067.0
32	13930.0
33	18114.0
34	24485.0
35	35442.0
36	59067.0
37	102719.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.85113717057127	20.57457947256905	11.631597558626598	27.94268579823308
2	21.368732750689972	21.371032658693654	33.67697792088317	23.58325666973321
3	17.02133164673413	25.078771849126035	32.46895124195032	25.430945262189514
4	12.456014259429622	22.21222401103956	35.44158233670653	29.890179392824283
5	13.315604875804969	35.70952161913524	36.5639374425023	14.410936062557496
6	24.57241920598915	42.695404925983745	18.212109973578972	14.520065894448134
7	24.37424170111955	31.905191795709246	22.76535429448735	20.95521220868385
8	21.18510553884899	39.93370400823381	18.75751076663006	20.123679686287137
9	23.083580115580347	18.477329576492913	20.866277565337395	37.57281274258935
10-11	22.5264425910433	27.464501234802157	26.394003950216916	23.615052223937624
12-13	22.765495630174794	24.635033348666052	26.49465271389144	26.104818307267706
14-15	23.960723095233988	26.09490624946096	23.154456959849128	26.78991369545593
16-17	24.646135048693715	26.582096524957777	24.165307075861573	24.60646135048694
18-19	21.659668486049878	27.04795610480452	26.33151107514192	24.96086433400368
20-21	22.745946412143514	27.09435372585097	26.361114305427787	23.798585556577738
22-23	23.953552222843534	26.505650005821785	25.741774393635996	23.799023377698685
24-25	21.180923412189017	29.028877654138018	25.71001022052471	24.08018871314825
26-27	22.712649890609683	28.767353674278468	25.53365513157025	22.9863413035416
28-29	21.836150882640446	28.203237306652866	27.148812604220574	22.811799206486114
30-31	22.05075056383925	27.641994776311417	27.221109796644132	23.08614486320519
32-33	20.581906310237912	28.921528463558626	26.820559482174964	23.676005744028497
34-35	21.82651145540743	28.478668993493894	26.850848266523304	22.843971284575378
36-37	23.536684776748555	26.89656263073979	25.99700098194119	23.56975161057046
38-39	23.043648894006633	27.08508537000858	26.848174072301074	23.02309166368371
40-41	21.756473540919792	27.40578256032245	27.260025471554368	23.57771842720339
42-43	20.59616661731433	27.4207320053243	27.577126199189856	24.40597517817151
44-45	19.83658425441566	26.538671082686815	28.718074776216167	24.906669886681357
46-47	21.008591803790054	26.530518368807886	28.137474610551372	24.323415216850687
48-49	21.008396113724864	27.306559490799636	27.792272010510594	23.892772384964907
50-51	22.949683825886456	26.739173246739433	26.35162816371767	23.959514763656436
52-53	19.576793359344673	27.617978303435624	27.626172738695274	25.179055598524425
54-55	19.629603540947596	27.19364952496503	27.54182162145844	25.63492531262893
56-57	19.78565113320251	27.03613453230687	27.781827068912225	25.39638726557839
58-59	20.11273493632963	27.35511361185945	27.424404914219668	25.107746537591254
60-61	19.703134569985075	26.509847110277857	27.445531059588745	26.341487260148316
62-63	19.56176404205248	26.74556178991873	27.42554344622754	26.267130721801244
64-65	20.18227406957987	26.767029067811478	26.552090012738194	26.49860684987046
66-67	19.610191324550154	28.03002421029024	25.845634959429077	26.514149505730533
68-69	19.801258628273423	28.84538536506832	25.469110709264918	25.88424529739333
70-71	20.244710915107277	28.207302803883405	25.72670114621995	25.821285134789374
72-73	19.80090419682453	27.916825392261856	25.790597215533783	26.49167319537983
74-75	19.544150943396225	27.45545372866128	25.906918238993708	27.093477088948788
76	19.232726425916184	26.563052534435023	26.013932133318384	28.19028890633041
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	3.5
14	6.5
15	8.0
16	13.0
17	16.0
18	29.0
19	41.5
20	57.0
21	73.0
22	98.0
23	198.5
24	362.5
25	451.0
26	678.0
27	956.5
28	1273.5
29	1539.0
30	1845.0
31	2535.0
32	3842.0
33	4765.0
34	5635.5
35	7471.5
36	9409.0
37	10381.0
38	11938.5
39	15645.5
40	19073.0
41	21482.5
42	22614.0
43	23463.5
44	25356.5
45	26198.0
46	25996.0
47	26077.0
48	25039.0
49	22735.5
50	21551.0
51	20174.5
52	16762.5
53	13527.5
54	12328.0
55	11211.5
56	8932.0
57	6668.0
58	5567.0
59	4739.5
60	3389.5
61	2493.0
62	2119.0
63	1831.5
64	1365.5
65	1045.5
66	799.0
67	694.0
68	636.5
69	536.0
70	460.0
71	427.0
72	371.0
73	298.5
74	247.0
75	212.0
76	187.5
77	159.0
78	130.0
79	105.0
80	91.0
81	70.0
82	62.0
83	61.0
84	47.0
85	29.0
86	21.0
87	17.0
88	13.5
89	10.5
90	10.0
91	8.5
92	8.0
93	8.5
94	6.0
95	2.5
96	2.0
97	3.0
98	7.5
99	9.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	8.624655013799448E-4
2	0.0
3	0.0
4	0.0
5	0.0
6	0.003162373505059798
7	0.006324747010119596
8	0.0017249310027598896
9	0.008624655013799448
10-11	0.003162373505059798
12-13	0.0
14-15	5.749770009199632E-4
16-17	7.18721251149954E-4
18-19	0.003306117755289788
20-21	0.0
22-23	0.0024436522539098434
24-25	0.0030186292548298067
26-27	8.624655013799448E-4
28-29	0.005749770009199632
30-31	4.312327506899724E-4
32-33	1.437442502299908E-4
34-35	0.003737350505979761
36-37	0.017105565777368905
38-39	0.008768399264029439
40-41	2.874885004599816E-4
42-43	2.874885004599816E-4
44-45	0.004456071757129715
46-47	0.0024436522539098434
48-49	1.437442502299908E-4
50-51	0.0027311407543698252
52-53	0.01236200551977921
54-55	0.006755979760809568
56-57	0.012218261269549219
58-59	0.009487120515179392
60-61	0.020986660533578654
62-63	0.010493330266789327
64-65	0.019261729530818766
66-67	0.015524379024839005
68-69	2.874885004599816E-4
70-71	2.874885004599816E-4
72-73	0.005031048758049678
74-75	0.007905933762649493
76	0.003162373505059798
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	347840.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.70087901556366
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.70829642923245	42.243907482555585
2	6.381017571244676	6.598084348942137
3	3.4355386955119784	5.328611113498565
4	2.069818827028029	4.28045811041248
5	1.294603312948318	3.346606462794445
6	0.8895293850003242	2.7593670668814307
7	0.6264206708869507	2.267054952286212
8	0.46912815800479246	1.9403470511840004
9	0.37749238723043055	1.7565019417347116
>10	2.6370576270530504	25.523651892133355
>50	0.10295370495838001	3.464930369713669
>100	0.008143230900662825	0.490479207863478
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0017249310027598896	0.0
2	0.0	0.0	0.0	0.0022999080036798527	0.0
3	0.0	0.0	0.0	0.003162373505059798	0.0
4	0.0	0.0	0.0	0.0040248390064397425	0.0
5	0.0	0.0	0.0	0.006037258509659613	0.0
6	0.0	0.0	0.0	0.006037258509659613	0.0
7	0.0	0.0	0.0	0.006037258509659613	0.0
8	0.0	0.0	0.0	0.006612235510579577	0.0
9	0.0	0.0	0.0	0.007762189512419504	0.0
10	0.0	0.0	0.0	0.008049678012879485	0.0
11	0.0	0.0	0.0	0.008049678012879485	0.0
12	0.0	0.0	0.0	0.008049678012879485	0.0
13	0.0	0.0	0.0	0.00891214351425943	0.0
14	0.0	0.0	0.0	0.010349586016559338	0.0
15	0.0	0.0	0.0	0.011499540018399264	0.0
16	0.0	0.0	0.0	0.012074517019319227	0.0
17	0.0	0.0	0.0	0.012362005519779208	0.0
18	0.0	0.0	0.0	0.012362005519779208	0.0
19	0.0	0.0	0.0	0.013799448022079117	0.0
20	0.0	0.0	0.0	0.01581186752529899	0.0
21	0.0	0.0	0.0	0.01581186752529899	0.0
22	0.0	0.0	0.0	0.027886384544618215	0.0
23	0.0	0.0	0.0	0.02903633854645814	0.0
24	0.0	0.0	0.0	0.031336246550137996	0.0
25	0.0	0.0	0.0	0.03421113155473781	0.0
26	0.0	0.0	0.0	0.0359360625574977	0.0
27	0.0	0.0	0.0	0.04542318307267709	0.0
28	0.0	0.0	0.0	0.06382244710211592	0.0
29	0.0	0.0	0.0	0.09429622815087396	0.0
30	0.0	0.0	0.0	0.1155703771849126	0.0
31	0.0	0.0	0.0	0.14546918123275068	0.0
32	0.0	0.0	0.0	0.1673183072677093	0.0
33	0.0	0.0	0.0	0.2018169273229071	0.0
34	0.0	0.0	0.0	0.24436522539098435	0.0
35	0.0	0.0	0.0	0.3096251149954002	0.0
36	0.0	0.0	0.0	0.42117065317387303	0.0
37	0.0	0.0	0.0	0.6807727690892365	0.0
38	0.0	0.0	0.0	1.063419963201472	0.0
39	0.0	0.0	0.0	1.5639374425022998	0.0
40	0.0	0.0	0.0	2.1193652253909843	0.0
41	0.0	0.0	0.0	2.6710556577736893	0.0
42	0.0	0.0	0.0	3.204921803127875	0.0
43	0.0	0.0	0.0	3.765524379024839	0.0
44	0.0	0.0	0.0	4.21256899724011	0.0
45	0.0	0.0	0.0	4.754484820607176	0.0
46	0.0	0.0	0.0	5.268226770929163	0.0
47	0.0	0.0	0.0	5.826816927322907	0.0
48	0.0	0.0	0.0	6.437442502299908	0.0
49	0.0	0.0	0.0	7.107003219871205	0.0
50	0.0	0.0	0.0	7.777713891444343	0.0
51	0.0	0.0	0.0	8.519146734130635	0.0
52	0.0	0.0	0.0	9.22291858325667	0.0
53	0.0	0.0	0.0	9.937614995400184	0.0
54	0.0	0.0	0.0	10.519204231830727	0.0
55	0.0	0.0	0.0	10.988960441582337	0.0
56	0.0	0.0	0.0	11.467053817847287	0.0
57	0.0	0.0	0.0	12.03081876724931	0.0
58	0.0	0.0	0.0	12.520986660533579	0.0
59	0.0	0.0	0.0	13.079576816927323	0.0
60	0.0	0.0	0.0	13.629254829806808	0.0
61	0.0	0.0	0.0	14.22550597976081	0.0
62	0.0	0.0	0.0	14.819457221711131	0.0
63	0.0	0.0	0.0	15.394721711131554	0.0
64	0.0	0.0	0.0	15.917950781968722	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATC	15	0.0022233422	70.002014	38
ACGAAGT	20	7.925369E-5	69.99194	24
AGATCTC	155	0.0	54.187317	40
GGTCCCC	20	0.00694719	52.50151	50
GTGTCGA	20	0.00694719	52.50151	36
AGCGAGA	20	0.00694719	52.50151	10
TGTGTCG	20	0.00694719	52.50151	35
GGTAGCC	20	0.00694719	52.50151	50
ACGTGGC	20	0.006951142	52.49396	15
CTCGGTG	110	0.0	50.903233	44
CGGTGGT	110	0.0	50.903233	46
GTAGATC	160	0.0	50.313946	38
TAGATCC	35	2.0858637E-5	49.99425	39
GATCTCG	155	0.0	49.671707	41
TCGGTGG	120	0.0	49.57763	45
TAGATCT	175	0.0	45.99471	39
ATCTCGG	155	0.0	45.156097	42
ACGCTTC	55	1.3102726E-7	44.54033	15
TCTCGGT	175	0.0	43.994938	43
CTTCCGA	40	4.591282E-5	43.744965	18
>>END_MODULE