FastQCFastQC Report
Wed 25 May 2016
SRR1779826_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779826_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347840
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT268997.733153173873045No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG35431.0185717571297148No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC21640.6221251149954002No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC7480.21504139834406627No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT6220.17881784728610856RNA PCR Primer, Index 48 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATC150.002225861769.98202533
ATTCGTC150.002225861769.98202529
TTCGTCG150.002225861769.98202530
CGTCGAT150.002225861769.98202532
TTAATAC501.4551915E-1162.983833
AAACTCG401.0664735E-861.2342836
GGTACAT308.359197E-658.343521
AATCTCG35950.052.5595236
GCCTGGA200.00693528652.5242858
GCGATCT200.00693923352.51672464
TGGAAAC200.006955038352.48652633
TCGTCGA200.006955038352.48652631
ATCTCGT36700.052.1528237
CGTCAAT35950.052.0728632
GTCAATC35750.052.07054533
TCTTCTG38400.051.98462750
TCTCGTA36450.051.9345438
TATGCCG38100.051.7977243
CCGTCTT38500.051.7511947
CTCGTAT37800.051.74597539