##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779826_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	347840
Sequences flagged as poor quality	0
Sequence length	76
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.521041283348666	34.0	34.0	34.0	34.0	34.0
2	33.59904553817847	34.0	34.0	34.0	34.0	34.0
3	33.73014029438822	34.0	34.0	34.0	34.0	34.0
4	33.50213028978841	34.0	34.0	34.0	34.0	34.0
5	33.57673068077277	34.0	34.0	34.0	34.0	34.0
6	37.22944457221711	38.0	38.0	38.0	36.0	38.0
7	37.310010349586015	38.0	38.0	38.0	37.0	38.0
8	37.19185257589697	38.0	38.0	38.0	36.0	38.0
9	37.096665133394666	38.0	38.0	38.0	36.0	38.0
10-11	37.02678530358786	38.0	37.5	38.0	35.5	38.0
12-13	37.212307382704694	38.0	38.0	38.0	36.0	38.0
14-15	37.23121693882244	38.0	38.0	38.0	36.0	38.0
16-17	37.15604300827967	38.0	38.0	38.0	36.0	38.0
18-19	37.02467082566697	38.0	37.5	38.0	35.5	38.0
20-21	37.02410447332107	38.0	37.5	38.0	35.5	38.0
22-23	37.01004484820608	38.0	37.5	38.0	35.5	38.0
24-25	37.2357506324747	38.0	38.0	38.0	36.0	38.0
26-27	37.13583831646734	38.0	38.0	38.0	36.0	38.0
28-29	36.628712913983435	38.0	37.0	38.0	34.0	38.0
30-31	36.98167117065317	38.0	37.0	38.0	36.0	38.0
32-33	37.01923298068077	38.0	37.5	38.0	36.0	38.0
34-35	37.08003967341306	38.0	38.0	38.0	36.0	38.0
36-37	37.036212051517936	38.0	38.0	38.0	35.5	38.0
38-39	36.685356773229074	38.0	37.0	38.0	34.5	38.0
40-41	36.86883624655013	38.0	37.0	38.0	35.0	38.0
42-43	37.15321699632015	38.0	38.0	38.0	36.0	38.0
44-45	36.92237954231831	38.0	37.5	38.0	35.5	38.0
46-47	37.06255174793009	38.0	38.0	38.0	36.0	38.0
48-49	36.68202621895124	38.0	37.0	38.0	34.5	38.0
50-51	36.88360596826127	38.0	37.0	38.0	35.5	38.0
52-53	36.81949603265869	38.0	37.0	38.0	35.0	38.0
54-55	36.82013138224471	38.0	37.0	38.0	35.0	38.0
56-57	36.690502817387305	38.0	37.0	38.0	34.5	38.0
58-59	36.817144376724926	38.0	37.0	38.0	35.0	38.0
60-61	36.47947619595216	38.0	37.0	38.0	34.0	38.0
62-63	36.29735798068077	38.0	37.0	38.0	34.0	38.0
64-65	36.66858469411223	38.0	37.0	38.0	34.0	38.0
66-67	36.46258480910764	38.0	37.0	38.0	34.0	38.0
68-69	36.594214293928246	38.0	37.0	38.0	34.0	38.0
70-71	35.98879226080957	38.0	37.0	38.0	32.5	38.0
72-73	34.91391875574977	38.0	37.0	38.0	23.5	38.0
74-75	34.76004341076357	38.0	37.0	38.0	23.5	38.0
76	34.53107463201472	38.0	36.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	0.0
22	6.0
23	19.0
24	54.0
25	142.0
26	209.0
27	502.0
28	930.0
29	1520.0
30	2641.0
31	4515.0
32	7255.0
33	11915.0
34	20019.0
35	38002.0
36	58416.0
37	201694.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.799359182699085	18.336348549388664	10.97126388844947	31.893028379462784
2	19.21774379024839	19.037488500459983	38.6709406623735	23.073827046918126
3	16.42335556577737	21.98769549218031	37.18491260349586	24.404036338546458
4	11.55502529898804	24.816294848206073	38.34751609935603	25.281163753449864
5	10.79950551977921	33.92048068077277	42.97809337626495	12.301920423183073
6	23.933130174793007	45.50885464581417	18.494422723091077	12.063592456301748
7	23.628679852805888	29.044388224471025	23.10372585096596	24.22320607175713
8	21.203426862925483	41.33739650413983	18.626667433302668	18.832509199632014
9	21.449804507819685	16.72608095676173	20.170768169273227	41.65334636614536
10-11	21.54870055197792	28.521302897884084	29.83785648574057	20.092140064397423
12-13	21.519951701931923	22.46737005519779	29.277397654093836	26.73528058877645
14-15	20.45739420423183	22.548441812327507	22.76837051517939	34.225793468261266
16-17	25.521935372585098	28.70127069917203	23.025097746090157	22.751696182152713
18-19	25.264489420423182	25.617669043238273	24.293928242870287	24.823913293468262
20-21	19.411798528058878	25.22064742410304	24.5197504599816	30.847803587856486
22-23	20.02069917203312	29.549793008279668	24.621521389144434	25.80798643054278
24-25	24.76357494519711	29.743055305997775	24.415136378337586	21.07823337046753
26-27	24.70230565777369	30.268083026678934	24.657169963201472	20.37244135234591
28-29	20.287488500459983	25.357779438822448	29.902685142594297	24.452046918123273
30-31	19.80723896044158	24.34840731370745	35.630893514259434	20.213460211591535
32-33	23.30324287028519	25.535734820607175	26.57227460901564	24.588747700091997
34-35	21.2140639374425	24.52607520699172	30.070722171113157	24.189138684452622
36-37	20.359073137074517	33.39567042318307	24.66996320147194	21.57529323827047
38-39	19.824488270469182	26.336821527138916	29.21630634774609	24.62238385464581
40-41	19.588747700091997	23.617467801287948	31.057957681692734	25.73582681692732
42-43	24.33636396305746	23.831818018471267	30.368922269738025	21.462895748733242
44-45	19.277253909843605	28.95699172033119	30.97127989880405	20.79447447102116
46-47	24.057612695492182	24.109648114075437	27.01328196872125	24.81945722171113
48-49	23.44598091076357	23.812385004599815	25.987810487580496	26.75382359705612
50-51	19.911870839742946	23.58310450421956	30.669235303420216	25.835789352617276
52-53	18.446113342219423	23.49364241971964	35.68555895374201	22.374685284318925
54-55	17.786065000848538	24.153116433726346	31.93305470621906	26.127763859206055
56-57	22.427763954608867	23.60784708538883	27.28530541213019	26.67908354787211
58-59	18.69499821693566	23.4229946277997	34.99795810373983	22.88404905152481
60-61	22.88112318481101	28.19105875180279	27.056974538824445	21.87084352456176
62-63	17.801630552000805	34.52298445655456	24.925589888852144	22.74979510259249
64-65	17.45720701940025	35.64375652353937	24.11718361373171	22.781852843328664
66-67	17.540365775976234	34.96267900203177	24.135203960583965	23.36175126140803
68-69	17.509347816398968	35.43415413941867	24.08034881263684	22.97614923154552
70-71	18.316093647792865	34.365605928822504	23.982115773054336	23.336184650330296
72-73	18.595359175756947	31.739856194547123	24.28546575742283	25.379318872273092
74-75	18.519274617656105	30.669745161577854	25.02566211122261	25.785318109543432
76	18.814027642402653	30.02021282407367	25.268042748829068	25.89771678469461
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	6.0
11	6.5
12	1.0
13	0.5
14	2.0
15	7.0
16	23.5
17	37.0
18	51.0
19	52.5
20	67.0
21	94.0
22	101.5
23	208.0
24	434.5
25	562.0
26	771.5
27	1015.0
28	1395.5
29	1742.0
30	1957.5
31	2466.0
32	3873.0
33	4987.0
34	5747.0
35	7160.0
36	8876.5
37	9940.0
38	11390.0
39	19209.0
40	28818.5
41	31498.5
42	30938.0
43	29301.5
44	27169.0
45	25620.0
46	24567.0
47	23951.5
48	22428.0
49	19928.0
50	18336.0
51	17503.5
52	13998.0
53	10773.0
54	10221.0
55	9296.5
56	7165.0
57	4845.5
58	3733.0
59	2997.5
60	1937.5
61	1394.5
62	1176.0
63	940.5
64	589.0
65	431.5
66	373.0
67	356.0
68	338.5
69	303.5
70	290.0
71	294.0
72	237.5
73	165.0
74	127.5
75	106.0
76	106.0
77	98.5
78	90.5
79	90.0
80	70.0
81	62.0
82	56.0
83	38.0
84	34.0
85	28.0
86	21.0
87	16.0
88	13.0
89	6.0
90	1.5
91	1.5
92	2.0
93	1.0
94	0.5
95	2.0
96	3.0
97	3.5
98	2.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.045710671573137074
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	7.18721251149954E-4
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	7.18721251149954E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.015811867525298985
52-53	0.03033003679852806
54-55	0.05261039558417663
56-57	0.05606025758969641
58-59	0.03564857405703772
60-61	0.032917433302667894
62-63	0.03018629254829807
64-65	0.017536798528058877
66-67	0.03262994480220791
68-69	0.00905588776448942
70-71	0.013655703771849126
72-73	0.022567847286108556
74-75	0.014661913523459063
76	0.011787028518859247
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	347840.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.820779668813248
#Duplication Level	Percentage of deduplicated	Percentage of total
1	42.485144736603004	6.721481140754369
2	10.492267994403155	3.319917203311867
3	9.3038469226436	4.415823367065317
4	7.506678054187639	4.750459981600736
5	5.9130308371645075	4.6774379024839
6	4.217622794424961	4.003564857405704
7	2.921989424142756	3.235970561177553
8	2.029765041522051	2.5689972401103955
9	1.6554305754938123	2.3571182152713894
>10	12.233104977194673	39.214293928242874
>50	1.0612200396140357	11.332509199632014
>100	0.17081281459540987	3.6347171113155476
>500	0.003634315204157657	0.39385924563017477
>1k	0.003634315204157657	1.640696872125115
>5k	0.0	0.0
>10k+	0.0018171576020788284	7.733153173873045
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT	26899	7.733153173873045	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG	3543	1.0185717571297148	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC	2164	0.6221251149954002	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC	748	0.21504139834406627	No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT	622	0.17881784728610856	RNA PCR Primer, Index 48 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0025873965041398345	0.0
2	0.0	0.0	0.0	0.0037373505059797607	0.0
3	0.0	0.0	0.0	0.004887304507819688	0.0
4	0.0	0.0	0.0	0.006612235510579577	0.0
5	0.0	0.0	0.0	0.00891214351425943	0.0
6	0.0	0.0	0.0	0.00891214351425943	0.0
7	0.0	0.0	0.0	0.00891214351425943	0.0
8	0.0	0.0	0.0	0.009487120515179392	0.0
9	0.0	0.0	0.0	0.011212051517939282	0.0
10	0.0	0.0	0.0	0.011499540018399264	0.0
11	0.0	0.0	0.0	0.011499540018399264	0.0
12	0.0	0.0	0.0	0.011787028518859245	0.0
13	0.0	0.0	0.0	0.012936982520699173	0.0
14	0.0	0.0	0.0	0.014086936522539098	0.0
15	0.0	0.0	0.0	0.015524379024839007	0.0
16	0.0	0.0	0.0	0.01609935602575897	0.0
17	0.0	0.0	0.0	0.01638684452621895	0.0
18	0.0	0.0	0.0	0.01638684452621895	0.0
19	0.0	0.0	0.0	0.017536798528058877	0.0
20	0.0	0.0	0.0	0.020411683532658695	0.0
21	0.0	0.0	0.0	0.022136614535418583	0.0
22	0.0	0.0	0.0	0.022999080036798528	0.0
23	0.0	0.0	0.0	0.026161453541858327	0.0
24	0.0	0.0	0.0	0.028461361545538178	0.0
25	0.0	0.0	0.0	0.031336246550137996	0.0
26	0.0	0.0	0.0	0.03392364305427783	0.0
27	0.0	0.0	0.0	0.045710671573137074	0.0
28	0.0	0.0	0.0	0.06525988960441582	0.0
29	0.0	0.0	0.0	0.10004599816007359	0.0
30	0.0	0.0	0.0	0.12390754369825208	0.0
31	0.0	0.0	0.0	0.15725620975160995	0.0
32	0.0	0.0	0.0	0.18226770929162833	0.0
33	0.0	0.0	0.0	0.21992870285188593	0.0
34	0.0	0.0	0.0	0.2670768169273229	0.0
35	0.0	0.0	0.0	0.3406738730450782	0.0
36	0.0	0.0	0.0	0.4605565777368905	0.0
37	0.0	0.0	0.0	0.7535073597056118	0.0
38	0.0	0.0	0.0	1.155703771849126	0.0
39	0.0	0.0	0.0	1.6820952161913523	0.0
40	0.0	0.0	0.0	2.2881209751609934	0.0
41	0.0	0.0	0.0	2.873735050597976	0.0
42	0.0	0.0	0.0	3.450149494020239	0.0
43	0.0	0.0	0.0	4.045538178472861	0.0
44	0.0	0.0	0.0	4.520469181232751	0.0
45	0.0	0.0	0.0	5.094871205151794	0.0
46	0.0	0.0	0.0	5.649149034038638	0.0
47	0.0	0.0	0.0	6.24798758049678	0.0
48	0.0	0.0	0.0	6.911798528058878	0.0
49	0.0	0.0	0.0	7.640294388224471	0.0
50	0.0	0.0	0.0	8.354703311867524	0.0
51	0.0	0.0	0.0	9.136097056117755	0.0
52	0.0	0.0	0.0	9.859418123275068	0.0
53	0.0	0.0	0.0	10.584176632934682	0.0
54	0.0	0.0	0.0	11.178127874885005	0.0
55	0.0	0.0	0.0	11.660533578656853	0.0
56	0.0	0.0	0.0	12.146389144434222	0.0
57	0.0	0.0	0.0	12.722228610855566	0.0
58	0.0	0.0	0.0	13.222458601655934	0.0
59	0.0	0.0	0.0	13.809797608095677	0.0
60	0.0	0.0	0.0	14.383624655013799	0.0
61	0.0	0.0	0.0	15.00057497700092	0.0
62	0.0	0.0	0.0	15.61810027598896	0.0
63	0.0	0.0	0.0	16.222401103955843	0.0
64	0.0	0.0	0.0	16.777541398344066	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATC	15	0.0022258617	69.982025	33
ATTCGTC	15	0.0022258617	69.982025	29
TTCGTCG	15	0.0022258617	69.982025	30
CGTCGAT	15	0.0022258617	69.982025	32
TTAATAC	50	1.4551915E-11	62.98383	3
AAACTCG	40	1.0664735E-8	61.23428	36
GGTACAT	30	8.359197E-6	58.34352	1
AATCTCG	3595	0.0	52.55952	36
GCCTGGA	20	0.006935286	52.52428	58
GCGATCT	20	0.006939233	52.516724	64
TGGAAAC	20	0.0069550383	52.486526	33
TCGTCGA	20	0.0069550383	52.486526	31
ATCTCGT	3670	0.0	52.15282	37
CGTCAAT	3595	0.0	52.07286	32
GTCAATC	3575	0.0	52.070545	33
TCTTCTG	3840	0.0	51.984627	50
TCTCGTA	3645	0.0	51.93454	38
TATGCCG	3810	0.0	51.79772	43
CCGTCTT	3850	0.0	51.75119	47
CTCGTAT	3780	0.0	51.745975	39
>>END_MODULE