FastQCFastQC Report
Wed 25 May 2016
SRR1779825_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779825_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194752
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGCCG150.002222802369.99460617
ACGACTC308.357911E-658.3288424
CGGTGGT900.054.4542346
GACGACT452.6897396E-854.4402523
TCGGTGG1100.054.08674245
GGTCGCA200.006938476652.50943850
CGTATCT200.00694552852.49595667
TCCGACT200.00694552852.49595620
GTCTCGT200.00694552852.49595643
GTCCGAC200.00694552852.49595619
GTAGATC1500.051.3293838
GGTGGTC1050.050.0089947
TGGTCGC751.4551915E-1146.67505649
AGCAATT451.6794274E-646.6630745
TCTCGGT1350.046.6630743
AGCGATC305.869546E-446.6630727
CTCGGTG1400.044.99653244
AGATCTC1750.043.99661340
TAGATCT1750.043.99661339
ATCTCGG1450.043.44492742
Produced by FastQC (version 0.11.5)