##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779825_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	194752
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.301958388103845	34.0	23.0	34.0	12.0	34.0
2	29.10129806112389	34.0	24.0	34.0	12.0	34.0
3	28.78567100722971	34.0	23.0	34.0	11.0	34.0
4	29.20878347847519	34.0	27.0	34.0	12.0	34.0
5	29.71593103023332	34.0	31.0	34.0	11.0	34.0
6	31.95786949556359	37.0	28.0	38.0	12.0	38.0
7	32.11690252218206	37.0	31.0	38.0	12.0	38.0
8	31.339575460072297	37.0	27.0	38.0	11.0	38.0
9	32.44734842260927	37.0	31.0	38.0	12.0	38.0
10-11	30.992500718862964	36.5	26.0	38.0	11.0	38.0
12-13	31.84449453664147	37.0	29.5	38.0	11.0	38.0
14-15	30.611480241537954	36.5	25.0	38.0	11.0	38.0
16-17	31.277280849490637	36.5	27.5	38.0	11.0	38.0
18-19	32.257876684193235	37.0	31.0	38.0	11.0	38.0
20-21	30.824322728393035	36.5	25.5	38.0	11.0	38.0
22-23	31.783848176141966	37.0	29.0	38.0	11.0	38.0
24-25	32.11321321475518	37.0	31.0	38.0	11.0	38.0
26-27	30.96208254600723	37.0	26.0	38.0	11.0	38.0
28-29	32.282697995399275	37.0	31.5	38.0	11.0	38.0
30-31	32.779042063752875	38.0	33.5	38.0	11.0	38.0
32-33	32.88449669323037	38.0	34.0	38.0	11.0	38.0
34-35	32.79454896483733	38.0	33.0	38.0	11.0	38.0
36-37	32.20362050197174	37.0	29.0	38.0	11.0	38.0
38-39	32.15471984883339	37.0	29.5	38.0	11.0	38.0
40-41	32.09126478803812	37.0	29.0	38.0	11.0	38.0
42-43	32.56278497781794	37.0	32.5	38.0	11.0	38.0
44-45	32.63862758790667	37.0	32.0	38.0	11.0	38.0
46-47	32.51052877505751	37.0	31.5	38.0	11.0	38.0
48-49	32.163754415872496	37.0	29.5	38.0	11.0	38.0
50-51	31.456839981104174	37.0	28.0	38.0	11.0	38.0
52-53	32.065277891883014	37.0	30.0	38.0	11.0	38.0
54-55	32.26206406095958	37.0	31.5	38.0	11.0	38.0
56-57	32.347020826487025	37.0	31.5	38.0	11.0	38.0
58-59	32.66114083552415	37.0	33.0	38.0	11.0	38.0
60-61	32.465697399769965	37.0	31.5	38.0	11.0	38.0
62-63	32.504266965165954	37.0	32.0	38.0	11.0	38.0
64-65	32.51081118550772	37.0	31.5	38.0	11.0	38.0
66-67	32.36077164804469	37.0	31.0	38.0	11.0	38.0
68-69	31.660439944134076	37.0	27.5	38.0	11.0	38.0
70-71	31.633551901906017	37.0	28.0	38.0	11.0	38.0
72-73	31.872406958593494	37.0	30.5	38.0	11.0	38.0
74-75	31.941381860006572	37.0	29.5	38.0	11.0	38.0
76	30.723212085113374	36.0	25.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	37.0
13	539.0
14	1605.0
15	2768.0
16	3849.0
17	4657.0
18	4825.0
19	4455.0
20	3735.0
21	2901.0
22	2546.0
23	2205.0
24	2357.0
25	2494.0
26	2875.0
27	3253.0
28	3976.0
29	4560.0
30	5550.0
31	6541.0
32	8222.0
33	10631.0
34	13510.0
35	19085.0
36	30634.0
37	46941.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.00909875123234	21.910429674663163	9.465884817614196	29.614586756490308
2	20.406979132435097	21.257804797896814	30.038715905356554	28.29650016431153
3	19.9499876766349	20.918912257640486	31.027152481104174	28.103947584620443
4	13.45043953335524	22.153302661846862	32.17425238251725	32.222005422280645
5	15.992133585277687	32.07155767334867	33.7803976339139	18.155911107459744
6	27.245605837317132	36.92996554504049	16.798718337124576	19.0257102805178
7	26.56646365886473	28.303669545748644	21.18487403588411	23.94499275950251
8	23.569767023893274	35.65145500947412	17.131472057758767	23.647305908873836
9	24.02869496451643	17.59800347133071	18.96958991054648	39.40371165360638
10-11	23.189042080669594	26.23481989268017	24.268402269634652	26.307735757015582
12-13	23.450336838646074	22.517868879395333	25.731186329280316	28.30060795267828
14-15	24.705263157894734	24.613350449293968	22.378177150192556	28.303209242618742
16-17	25.491973104186616	25.59364112728272	23.042954739758102	25.871431028772562
18-19	22.199058265339133	25.665607172382632	25.355204444741126	26.78013011753711
20-21	24.329660285902072	24.858538038120276	24.9437746467302	25.868027029247454
22-23	25.31599095224769	24.94216781475215	23.8361032018876	25.905738031112563
24-25	22.12594321335685	27.89446793652261	23.45947167214815	26.52011717797239
26-27	23.935363120316097	27.670674379138326	24.11482442830405	24.279138072241523
28-29	22.331769714055373	27.042654198792754	25.90806740251771	24.71750868463417
30-31	22.980113067455367	26.480480202925786	25.814501594343547	24.7249051352753
32-33	21.47577431810713	27.53886994742031	25.58895415708183	25.39640157739073
34-35	21.793281932387988	28.052027410414208	25.54334291003294	24.61134774716486
36-37	23.724835661462613	25.75518693508628	25.321744042728017	25.198233360723087
38-39	23.507466518774137	25.602610713992274	26.34618149700107	24.54374127023252
40-41	22.210611985016804	26.466805133721692	25.92329199004886	25.39929089121265
42-43	20.543819832402235	26.081375287545182	27.07674375616168	26.298061123890896
44-45	20.377364303668124	24.48066835947429	28.24455507315958	26.897412263698005
46-47	21.006485337386454	25.11591962905719	27.51903752047528	26.358557513081077
48-49	21.046767170555373	25.424385885639172	27.918840371344068	25.610006572461387
50-51	22.91434527874627	25.476130569405438	26.12184013104181	25.487684020806483
52-53	18.832500994954618	26.710487463571823	27.172403168449026	27.284608373024533
54-55	19.3463491786358	25.642790019359445	27.04493753113173	27.965923270873024
56-57	19.829967955301754	26.216568752310916	26.519298714103773	27.434164578283553
58-59	19.534583960272993	26.21206908061973	26.642410734918787	27.610936224188485
60-61	19.085868948356396	25.471022215146156	26.734699642287612	28.708409194209832
62-63	19.679766654855623	25.57963981451526	26.51476637241762	28.225827158211498
64-65	20.409986980871896	25.12383578950612	25.606082783166034	28.860094446455946
66-67	19.931389717701556	27.372472691977833	24.03439758015232	28.66174001016829
68-69	19.851194984377525	28.142787090214966	23.663489113049195	28.342528812358314
70-71	19.572478632917502	27.806603834136496	24.147819902901404	28.4730976300446
72-73	19.69616444194543	27.370618274807633	24.418530015122457	28.514687268124483
74-75	19.344096457309526	26.69727173293485	24.301244229455836	29.657387580299787
76	18.876992990834168	25.984235795527482	24.14850188708311	30.990269326555236
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.5
12	3.0
13	6.5
14	6.5
15	5.0
16	13.5
17	20.0
18	25.0
19	46.5
20	78.5
21	94.0
22	110.0
23	155.5
24	259.5
25	334.0
26	422.0
27	582.0
28	852.5
29	1051.0
30	1217.0
31	1556.5
32	2119.5
33	2509.0
34	2930.0
35	3831.5
36	4742.5
37	5173.0
38	5720.0
39	6971.0
40	8235.5
41	9378.0
42	9960.0
43	10252.0
44	11037.5
45	11733.0
46	11935.0
47	12033.5
48	11946.5
49	11418.0
50	11075.0
51	10537.0
52	9413.5
53	8359.0
54	7890.0
55	7366.0
56	6351.0
57	5383.0
58	4906.0
59	4416.5
60	3611.0
61	3078.5
62	2862.0
63	2619.5
64	2215.5
65	1935.0
66	1668.5
67	1521.0
68	1443.5
69	1277.0
70	1109.5
71	1031.0
72	988.0
73	874.5
74	797.0
75	790.0
76	708.5
77	564.5
78	475.0
79	448.0
80	417.5
81	350.0
82	276.0
83	239.0
84	208.5
85	170.0
86	129.0
87	96.0
88	84.5
89	64.5
90	51.5
91	38.0
92	29.0
93	27.5
94	19.5
95	11.0
96	9.0
97	10.5
98	8.0
99	5.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0025673677292145907
7	0.007188629641800854
8	0.0056482090042720996
9	0.007188629641800854
10-11	0.003594314820900427
12-13	0.0
14-15	0.0010269470916858365
16-17	2.567367729214591E-4
18-19	0.0025673677292145907
20-21	0.0
22-23	0.003851051593821886
24-25	0.0028241045021360498
26-27	0.0012836838646072954
28-29	0.005904945777193559
30-31	5.134735458429182E-4
32-33	0.0
34-35	0.004364525139664804
36-37	0.016431153466973384
38-39	0.008215576733486692
40-41	2.567367729214591E-4
42-43	0.0
44-45	0.0033375780479789684
46-47	0.0025673677292145907
48-49	0.0
50-51	0.0025673677292145907
52-53	0.010012734143936905
54-55	0.007702103187643772
56-57	0.012323365100230037
58-59	0.011809891554387117
60-61	0.021822625698324022
62-63	0.009755997371015445
64-65	0.01976873151495235
66-67	0.014890732829444628
68-69	2.567367729214591E-4
70-71	7.702103187643773E-4
72-73	0.004364525139664804
74-75	0.006675156095957937
76	0.003594314820900427
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	194752.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.27782025952832
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.06611299971097	48.132978262366805
2	11.123966471116505	14.078007018646597
3	5.741327275140382	10.898960261023817
4	3.046160531997641	7.710175945016634
5	1.5198274603548136	4.808568443091365
6	0.7992620966513299	3.034533797529396
7	0.45777788656246765	2.02770307772806
8	0.30151808961478255	1.526352598371245
9	0.2066055596728323	1.1766194522637063
>10	0.7364531028426174	6.564816961382795
>50	9.88526335621138E-4	0.0412841825795814
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0015404206375287544	0.0
3	0.0	0.0	0.0	0.0015404206375287544	0.0
4	0.0	0.0	0.0	0.0020538941833716726	0.0
5	0.0	0.0	0.0	0.004621261912586263	0.0
6	0.0	0.0	0.0	0.004621261912586263	0.0
7	0.0	0.0	0.0	0.004621261912586263	0.0
8	0.0	0.0	0.0	0.004621261912586263	0.0
9	0.0	0.0	0.0	0.00821557673348669	0.0
10	0.0	0.0	0.0	0.009242523825172527	0.0
11	0.0	0.0	0.0	0.011296418008544199	0.0
12	0.0	0.0	0.0	0.01386378573775879	0.0
13	0.0	0.0	0.0	0.015404206375287546	0.0
14	0.0	0.0	0.0	0.01643115346697338	0.0
15	0.0	0.0	0.0	0.0169446270128163	0.0
16	0.0	0.0	0.0	0.01951199474203089	0.0
17	0.0	0.0	0.0	0.01951199474203089	0.0
18	0.0	0.0	0.0	0.02002546828787381	0.0
19	0.0	0.0	0.0	0.022079362471245482	0.0
20	0.0	0.0	0.0	0.023106309562931318	0.0
21	0.0	0.0	0.0	0.02516020374630299	0.0
22	0.0	0.0	0.0	0.03440272757147552	0.0
23	0.0	0.0	0.0	0.035429674663161353	0.0
24	0.0	0.0	0.0	0.04826651330923431	0.0
25	0.0	0.0	0.0	0.051347354584291814	0.0
26	0.0	0.0	0.0	0.05699556358856392	0.0
27	0.0	0.0	0.0	0.09755997371015446	0.0
28	0.0	0.0	0.0	0.1386378573775879	0.0
29	0.0	0.0	0.0	0.18331005586592178	0.0
30	0.0	0.0	0.0	0.22387446598751232	0.0
31	0.0	0.0	0.0	0.26187150837988826	0.0
32	0.0	0.0	0.0	0.30140897140979295	0.0
33	0.0	0.0	0.0	0.3337578047978968	0.0
34	0.0	0.0	0.0	0.3840782122905028	0.0
35	0.0	0.0	0.0	0.5247699638514624	0.0
36	0.0	0.0	0.0	0.7250246467302005	0.0
37	0.0	0.0	0.0	1.0844561288202432	0.0
38	0.0	0.0	0.0	1.5835524153795597	0.0
39	0.0	0.0	0.0	2.1447790009858694	0.0
40	0.0	0.0	0.0	2.8076733486690766	0.0
41	0.0	0.0	0.0	3.664660696680907	0.0
42	0.0	0.0	0.0	4.59764212947749	0.0
43	0.0	0.0	0.0	5.447954321393362	0.0
44	0.0	0.0	0.0	6.353721656260269	0.0
45	0.0	0.0	0.0	7.136768813670719	0.0
46	0.0	0.0	0.0	7.968082484390404	0.0
47	0.0	0.0	0.0	8.867688136707196	0.0
48	0.0	0.0	0.0	9.667679921130464	0.0
49	0.0	0.0	0.0	10.361382681564246	0.0
50	0.0	0.0	0.0	11.017088399605653	0.0
51	0.0	0.0	0.0	11.854563752875451	0.0
52	0.0	0.0	0.0	12.691012159053566	0.0
53	0.0	0.0	0.0	13.602941176470589	0.0
54	0.0	0.0	0.0	14.535922609267171	0.0
55	0.0	0.0	0.0	15.340535655603023	0.0
56	0.0	0.0	0.0	16.080451035162667	0.0
57	0.0	0.0	0.0	16.765938218862964	0.0
58	0.0	0.0	0.0	17.458614032205062	0.0
59	0.0	0.0	0.0	18.087619125862634	0.0
60	0.0	0.0	0.0	18.733568846533025	0.0
61	0.0	0.0	0.0	19.402111403220506	0.0
62	0.0	0.0	0.0	20.075275221820572	0.0
63	0.0	0.0	0.0	20.730467466316135	0.0
64	0.0	0.0	0.0	21.390794446270128	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGCCG	15	0.0022228023	69.994606	17
ACGACTC	30	8.357911E-6	58.32884	24
CGGTGGT	90	0.0	54.45423	46
GACGACT	45	2.6897396E-8	54.44025	23
TCGGTGG	110	0.0	54.086742	45
GGTCGCA	20	0.0069384766	52.509438	50
CGTATCT	20	0.006945528	52.495956	67
TCCGACT	20	0.006945528	52.495956	20
GTCTCGT	20	0.006945528	52.495956	43
GTCCGAC	20	0.006945528	52.495956	19
GTAGATC	150	0.0	51.32938	38
GGTGGTC	105	0.0	50.00899	47
TGGTCGC	75	1.4551915E-11	46.675056	49
AGCAATT	45	1.6794274E-6	46.663074	5
TCTCGGT	135	0.0	46.66307	43
AGCGATC	30	5.869546E-4	46.66307	27
CTCGGTG	140	0.0	44.996532	44
AGATCTC	175	0.0	43.996613	40
TAGATCT	175	0.0	43.996613	39
ATCTCGG	145	0.0	43.444927	42
>>END_MODULE