FastQCFastQC Report
Wed 25 May 2016
SRR1779825_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779825_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194752
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT2038910.469212126191259No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG26511.3612183700295761No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC15730.8076938876109102No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT5680.2916529740387775TruSeq Adapter, Index 2 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC4490.23054962208347027No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTACCTCGTATGCCGT3730.19152563259940847No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTAACTCGTATGCCGT3350.17201363785737758No Hit
CTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC2150.1103968123562274No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATA150.00222393469.9856329
ATCCGTG150.00222393469.9856321
TAACCGG150.00222393469.9856322
CGAGGAT150.00222393469.9856319
TCTAATG308.351542E-658.3363369
CGTCTGC252.3885147E-455.988541
AGACGAT252.3885147E-455.988525
TACCTCG700.054.98870536
ATCTCGT28950.053.18423537
CTCGTAT30300.053.0089139
CGTATGC30850.052.9712941
TCGTATG31050.052.96818540
TCTCGTA28950.052.94248638
CCGTCTT30800.052.74352647
GCCGTCT30900.052.68614246
TATGCCG30900.052.65908443
GCTCGAA200.006934951552.51618261
TATAAGG200.00694200152.50269768
CGTATGA200.006949056452.48921641
CCGATAT200.006949056452.48921630