##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779825_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	194752
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.47994372329938	34.0	34.0	34.0	34.0	34.0
2	33.58412237101545	34.0	34.0	34.0	34.0	34.0
3	33.71695284258955	34.0	34.0	34.0	34.0	34.0
4	33.47459332895169	34.0	34.0	34.0	34.0	34.0
5	33.552102160696684	34.0	34.0	34.0	34.0	34.0
6	37.17562335688466	38.0	38.0	38.0	36.0	38.0
7	37.261055085441996	38.0	38.0	38.0	36.0	38.0
8	37.14023476010516	38.0	38.0	38.0	36.0	38.0
9	37.03999445448571	38.0	38.0	38.0	36.0	38.0
10-11	36.97901433618139	38.0	37.0	38.0	35.0	38.0
12-13	37.184164989319754	38.0	38.0	38.0	36.0	38.0
14-15	37.21344838563917	38.0	38.0	38.0	36.0	38.0
16-17	37.11953664147224	38.0	38.0	38.0	36.0	38.0
18-19	36.96873716316135	38.0	37.5	38.0	35.5	38.0
20-21	36.97888083305948	38.0	37.5	38.0	35.5	38.0
22-23	36.96676029000986	38.0	37.0	38.0	35.5	38.0
24-25	37.17207525468288	38.0	38.0	38.0	36.0	38.0
26-27	37.073488333881045	38.0	38.0	38.0	36.0	38.0
28-29	36.48071136625041	38.0	37.0	38.0	34.0	38.0
30-31	36.715979810220176	38.0	37.0	38.0	34.0	38.0
32-33	36.83071033930332	38.0	37.0	38.0	35.0	38.0
34-35	37.005853598422604	38.0	38.0	38.0	36.0	38.0
36-37	36.88003204074926	38.0	37.5	38.0	35.5	38.0
38-39	36.31342938711798	38.0	37.0	38.0	34.0	38.0
40-41	36.68768228310877	38.0	37.0	38.0	34.5	38.0
42-43	37.056348586920805	38.0	37.5	38.0	36.0	38.0
44-45	36.77373531465659	38.0	37.0	38.0	34.5	38.0
46-47	36.935731083634565	38.0	37.5	38.0	35.5	38.0
48-49	36.53859010433783	38.0	37.0	38.0	34.0	38.0
50-51	36.71487327472889	38.0	37.0	38.0	34.5	38.0
52-53	36.640306646401584	38.0	37.0	38.0	34.0	38.0
54-55	36.64637847108117	38.0	37.0	38.0	34.0	38.0
56-57	36.49737101544528	38.0	37.0	38.0	34.0	38.0
58-59	36.61139808577062	38.0	37.0	38.0	34.0	38.0
60-61	36.22467805208676	38.0	37.0	38.0	34.0	38.0
62-63	36.06808659217877	38.0	37.0	38.0	34.0	38.0
64-65	36.48725558659218	38.0	37.0	38.0	34.0	38.0
66-67	36.23064718205718	38.0	37.0	38.0	33.0	38.0
68-69	36.344163346204404	38.0	37.0	38.0	34.0	38.0
70-71	35.53181482090043	38.0	36.5	38.0	29.5	38.0
72-73	34.025265465823196	38.0	35.5	38.0	22.0	38.0
74-75	33.79427168912258	38.0	35.5	38.0	19.0	38.0
76	33.506618674005914	38.0	34.0	38.0	19.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	4.0
22	7.0
23	24.0
24	47.0
25	102.0
26	211.0
27	453.0
28	773.0
29	1279.0
30	1997.0
31	3164.0
32	4939.0
33	7761.0
34	13057.0
35	23934.0
36	33165.0
37	103833.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.857841525563046	16.562037645898407	10.772850567154364	35.80727026138418
2	19.081703910614525	17.37029658232008	39.720773085770624	23.827226421294775
3	17.47299129148866	18.97746878080841	38.1269511994742	25.422588728228725
4	12.56007640486362	26.135290009858693	33.40812931317779	27.896504272099904
5	12.16932303647716	31.184788859677948	42.494043706868226	14.151844396976667
6	26.107562438383177	43.2930085441998	17.334866907656917	13.264562109760107
7	25.445695037791655	26.884961386789353	19.37951856720342	28.289825008215573
8	22.468575418994412	42.02729625369701	16.715617811370358	18.78851051593822
9	21.046767170555373	16.720239073282944	17.716377752218207	44.516616003943476
10-11	21.952021031876438	28.43180044364114	28.34322625698324	21.27295226749918
12-13	22.036230693394675	19.82392992113046	29.540646565888924	28.599192819585934
14-15	20.26474696023661	20.133554469273744	21.295545103516268	38.30615346697338
16-17	26.794333305948076	28.98250082155767	21.284248685507723	22.938917186986526
18-19	27.184316464015772	24.072923512980612	22.352787134406835	26.38997288859678
20-21	20.38592671705554	22.511193723299375	22.18642170555373	34.91645785409136
22-23	20.253707279000984	29.322163572132766	21.988477653631286	28.435651495234964
24-25	26.82675101347101	29.2680672549377	22.190072990451934	21.71510874113935
26-27	27.25979707525468	30.262076897798224	22.093996467302006	20.384129559645086
28-29	26.455954239237595	23.8562376766349	22.814399852119617	26.87340823200789
30-31	27.43848586920802	29.25130422280644	22.345855241537958	20.964354666447583
32-33	21.231874383831745	29.403292392375945	22.41003943476832	26.954793789023988
34-35	25.786641472231352	24.062140568517908	22.841870686822215	27.309347272428525
36-37	21.368201610253042	28.358373726585604	29.30906999671377	20.964354666447583
38-39	19.4152049786395	23.99872658560631	30.207391965165954	26.378676470588236
40-41	19.51712947748932	21.46730200460072	30.784279493920476	28.231289023989486
42-43	26.089724828113848	21.122612977597033	30.489188758979413	22.298473435309702
44-45	19.466809069996714	27.773013884324683	31.685939040420635	21.07423800525797
46-47	25.469828294446273	21.29220752546829	26.152748110417352	27.08521606966809
48-49	24.88703581991456	20.90196763062767	25.00025673677292	29.21073981268485
50-51	19.115517347630494	21.409019946180237	31.572122594030528	27.903340112158748
52-53	17.426777436792975	21.446603063727064	38.434751345035245	22.691868154444716
54-55	16.870527435155836	21.762929019464806	32.668923604868	28.697619940511355
56-57	23.78212307723924	21.448094450324184	26.15160140154746	28.618181070889122
58-59	17.38064052186866	21.43539563910933	37.301538382515346	23.882425456506663
60-61	23.918715555806656	27.63239667384013	25.684261347002295	22.76462642335092
62-63	17.375158900345408	36.40005649790059	22.976155317864894	23.24862928388911
64-65	17.19870994910667	37.23917810611079	21.9339465183519	23.628165426430638
66-67	17.127249192268376	37.240151838135205	21.29124054222035	24.34135842737607
68-69	17.125712833179705	37.51331948534792	21.293203275245396	24.06776440622697
70-71	17.890404135072885	36.470757403408406	20.883093806891274	24.755744654627435
72-73	18.869421640401715	33.4309976914919	20.805076149952882	26.8945045181535
74-75	19.455899345230453	32.133008088329696	20.76954679676467	27.641545769675187
76	19.879935705518953	32.79172593707152	20.34725182176255	26.981086535646977
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	7.0
16	29.0
17	45.0
18	40.0
19	60.0
20	92.5
21	100.0
22	131.5
23	192.0
24	287.5
25	354.0
26	443.5
27	656.5
28	957.5
29	1135.0
30	1271.0
31	1593.5
32	2206.5
33	2633.0
34	2906.5
35	3594.0
36	4441.5
37	4875.0
38	5362.5
39	6669.5
40	7896.0
41	11753.5
42	15204.0
43	16821.5
44	18007.0
45	15975.0
46	14375.0
47	12946.5
48	10739.5
49	9553.5
50	9146.0
51	8707.0
52	7495.0
53	6411.0
54	6100.0
55	5640.5
56	4770.5
57	3969.0
58	3578.0
59	3226.0
60	2586.0
61	2132.0
62	1966.0
63	1863.0
64	1691.0
65	1501.5
66	1293.5
67	1206.0
68	1169.0
69	1057.0
70	978.5
71	975.0
72	911.5
73	788.5
74	711.0
75	693.0
76	663.5
77	570.0
78	455.0
79	404.0
80	392.0
81	339.0
82	260.5
83	223.0
84	194.0
85	138.5
86	87.0
87	62.0
88	52.0
89	29.0
90	12.5
91	5.5
92	2.0
93	7.0
94	6.0
95	0.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04929346040092015
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	2.567367729214591E-4
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	5.134735458429182E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.014377259283601708
52-53	0.02798430824843904
54-55	0.04775303976339139
56-57	0.05648209004272099
58-59	0.03440272757147552
60-61	0.027214097929674663
62-63	0.02798430824843904
64-65	0.015917679921130464
66-67	0.036456621754847186
68-69	0.009499260598093986
70-71	0.014120522510680248
72-73	0.01925525796910943
74-75	0.013863785737758792
76	0.011809891554387117
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	194752.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.767108938547487
#Duplication Level	Percentage of deduplicated	Percentage of total
1	39.34759694509681	10.925690108445613
2	16.942507905394162	9.408889254025631
3	14.418329419161566	12.010659710811698
4	10.614494147234499	11.789352612553401
5	6.542522699114225	9.083347025961222
6	3.885200732289143	6.472847518895826
7	2.267137600088762	4.406629970423924
8	1.370268321097694	3.043871179756819
9	0.9357027941638775	2.338358527768649
>10	3.6004216210218765	15.63372905027933
>50	0.0536272352386412	0.9684111074597437
>100	0.014793720065832055	0.9884365757476175
>500	0.0018492150082290069	0.2916529740387775
>1k	0.0036984300164580137	2.1689122576404865
>5k	0.0	0.0
>10k+	0.0018492150082290069	10.469212126191259
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT	20389	10.469212126191259	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG	2651	1.3612183700295761	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC	1573	0.8076938876109102	No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT	568	0.2916529740387775	TruSeq Adapter, Index 2 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC	449	0.23054962208347027	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTACCTCGTATGCCGT	373	0.19152563259940847	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTAACTCGTATGCCGT	335	0.17201363785737758	No Hit
CTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC	215	0.1103968123562274	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0015404206375287544	0.0
3	0.0	0.0	0.0	0.0015404206375287544	0.0
4	0.0	0.0	0.0	0.0025673677292145907	0.0
5	0.0	0.0	0.0	0.006675156095957936	0.0
6	0.0	0.0	0.0	0.006675156095957936	0.0
7	0.0	0.0	0.0	0.006675156095957936	0.0
8	0.0	0.0	0.0	0.006675156095957936	0.0
9	0.0	0.0	0.0	0.010269470916858363	0.0
10	0.0	0.0	0.0	0.011296418008544199	0.0
11	0.0	0.0	5.134735458429181E-4	0.01437725928360171	0.0
12	0.0	0.0	5.134735458429181E-4	0.018998521196187973	0.0
13	0.0	0.0	5.134735458429181E-4	0.021052415379559646	0.0
14	0.0	0.0	5.134735458429181E-4	0.022079362471245482	0.0
15	0.0	0.0	5.134735458429181E-4	0.022592836017088398	0.0
16	0.0	0.0	5.134735458429181E-4	0.02516020374630299	0.0
17	0.0	0.0	5.134735458429181E-4	0.02516020374630299	0.0
18	0.0	0.0	5.134735458429181E-4	0.025673677292145907	0.0
19	0.0	0.0	5.134735458429181E-4	0.02772757147551758	0.0
20	0.0	0.0	5.134735458429181E-4	0.02875451856720342	0.0
21	0.0	0.0	5.134735458429181E-4	0.029267992113046336	0.0
22	0.0	0.0	5.134735458429181E-4	0.0338892540256326	0.0
23	0.0	0.0	5.134735458429181E-4	0.034916201117318434	0.0
24	0.0	0.0	5.134735458429181E-4	0.04210483075911929	0.0
25	0.0	0.0	5.134735458429181E-4	0.046726092671705556	0.0
26	0.0	0.0	5.134735458429181E-4	0.052374301675977654	0.0
27	0.0	0.0	5.134735458429181E-4	0.09910039434768321	0.0
28	0.0	0.0	5.134735458429181E-4	0.14223217219848833	0.0
29	0.0	0.0	5.134735458429181E-4	0.1920391061452514	0.0
30	0.0	0.0	5.134735458429181E-4	0.24184604009201446	0.0
31	0.0	0.0	5.134735458429181E-4	0.282410450213605	0.0
32	0.0	0.0	5.134735458429181E-4	0.32605570161025305	0.0
33	0.0	0.0	5.134735458429181E-4	0.36610663818600064	0.0
34	0.0	0.0	5.134735458429181E-4	0.42053483404534997	0.0
35	0.0	0.0	5.134735458429181E-4	0.5817655274400263	0.0
36	0.0	0.0	5.134735458429181E-4	0.8102612553401248	0.0
37	0.0	0.0	5.134735458429181E-4	1.2087167269142294	0.0
38	0.0	0.0	5.134735458429181E-4	1.7540256325994086	0.0
39	0.0	0.0	5.134735458429181E-4	2.3496549457771936	0.0
40	0.0	0.0	5.134735458429181E-4	3.076733486690766	0.0
41	0.0	0.0	5.134735458429181E-4	4.016390075583306	0.0
42	0.0	0.0	5.134735458429181E-4	5.048985376273414	0.0
43	0.0	0.0	5.134735458429181E-4	5.984534176799212	0.0
44	0.0	0.0	5.134735458429181E-4	6.967836017088399	0.0
45	0.0	0.0	5.134735458429181E-4	7.822255997371015	0.0
46	0.0	0.0	5.134735458429181E-4	8.736752382517253	0.0
47	0.0	0.0	5.134735458429181E-4	9.716973381531384	0.0
48	0.0	0.0	5.134735458429181E-4	10.595526618468616	0.0
49	0.0	0.0	5.134735458429181E-4	11.357007886953664	0.0
50	0.0	0.0	5.134735458429181E-4	12.069195695037791	0.0
51	0.0	0.0	5.134735458429181E-4	12.961612717712784	0.0
52	0.0	0.0	5.134735458429181E-4	13.830409957279	0.0
53	0.0	0.0	5.134735458429181E-4	14.764931810713112	0.0
54	0.0	0.0	5.134735458429181E-4	15.72718123562274	0.0
55	0.0	0.0	5.134735458429181E-4	16.549765856063097	0.0
56	0.0	0.0	5.134735458429181E-4	17.30559891554387	0.0
57	0.0	0.0	5.134735458429181E-4	18.013678935261254	0.0
58	0.0	0.0	5.134735458429181E-4	18.71611074597437	0.0
59	0.0	0.0	5.134735458429181E-4	19.37797814656589	0.0
60	0.0	0.0	5.134735458429181E-4	20.046007229707527	0.0
61	0.0	0.0	5.134735458429181E-4	20.75254682878738	0.0
62	0.0	0.0	5.134735458429181E-4	21.465248110417352	0.0
63	0.0	0.0	5.134735458429181E-4	22.167679921130464	0.0
64	0.0	0.0	5.134735458429181E-4	22.871652152481104	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGATA	15	0.002223934	69.98563	29
ATCCGTG	15	0.002223934	69.98563	21
TAACCGG	15	0.002223934	69.98563	22
CGAGGAT	15	0.002223934	69.98563	19
TCTAATG	30	8.351542E-6	58.33633	69
CGTCTGC	25	2.3885147E-4	55.9885	41
AGACGAT	25	2.3885147E-4	55.9885	25
TACCTCG	70	0.0	54.988705	36
ATCTCGT	2895	0.0	53.184235	37
CTCGTAT	3030	0.0	53.00891	39
CGTATGC	3085	0.0	52.97129	41
TCGTATG	3105	0.0	52.968185	40
TCTCGTA	2895	0.0	52.942486	38
CCGTCTT	3080	0.0	52.743526	47
GCCGTCT	3090	0.0	52.686142	46
TATGCCG	3090	0.0	52.659084	43
GCTCGAA	20	0.0069349515	52.516182	61
TATAAGG	20	0.006942001	52.502697	68
CGTATGA	20	0.0069490564	52.489216	41
CCGATAT	20	0.0069490564	52.489216	30
>>END_MODULE