FastQCFastQC Report
Wed 25 May 2016
SRR1779824_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779824_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44271
Sequences flagged as poor quality0
Sequence length76
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT1855441.91005398567911No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG23085.213345079171466No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC13353.01551805922613No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT5161.1655485532289762RNA PCR Primer, Index 47 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC4871.100042917485487No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCGT3040.6866797677938153No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGT2920.6595739874861647No Hit
CTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC2280.5150098258453615No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATATCGTATGCCGT1650.37270447923019584No Hit
CTTTACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC1600.3614104041020081No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAGCTCGTATGCCGT1230.27783424815341873No Hit
CTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG1150.2597637279483183No Hit
CTTATACACATCTCCGAGCCCACGAGACCATGGTAGATCTCGTATGCCGT880.19877572225610443No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCG870.1965169072304669No Hit
TATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCT780.17618757199972895No Hit
CCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG750.1694111269228163No Hit
CTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG710.16037586682026608No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC690.155858236768991No Hit
TTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC620.14004653158952812No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCAGT570.12875245646134037No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGGTAGATCTCGTATGCCGT550.12423482641006527No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTCGATCTCGTATGCCGT520.11745838133315263No Hit
CTTATACACATCTCCGAGCCCACGAGAAAATGGTAGATCTCGTATGCCGT520.11745838133315263No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGGTAGATCTCGTATGCCGT490.11068193625623998No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATATCGTATGCCG480.10842312123060242No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTGAA207.777702E-570.1211756
TCTGATT207.777702E-570.1211753
CAGTCTT150.002205504370.04185547
AGTCTTC150.002205504370.04185548
GTATGCA150.002215432269.96271542
CAATGGC150.002215432269.9627159
TATGCAG150.002215432269.96271543
CTCTCGT150.002215432269.96271537
TAGCTCT150.002215432269.96271534
ATGAATA150.002215432269.96271525
TTTGGTA150.002215432269.9627155
GTAGCTC150.002215432269.96271533
AATGAGT150.002215432269.96271531
ATATATG150.002215432269.96271537
GAGACAT150.002215432269.96271524
AGCTCTC207.86554E-569.9627135
CGAGACG207.86554E-569.9627123
GCTCTCG207.86554E-569.9627136
GAGACGA207.86554E-569.9627124
TCTTTAT1000.059.4683071
Produced by FastQC (version 0.11.5)