##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779824_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	44271
Sequences flagged as poor quality	0
Sequence length	76
%GC	44
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.473199159720814	34.0	34.0	34.0	34.0	34.0
2	33.59723069277857	34.0	34.0	34.0	34.0	34.0
3	33.71981658421992	34.0	34.0	34.0	34.0	34.0
4	33.48020148630029	34.0	34.0	34.0	34.0	34.0
5	33.56163176797452	34.0	34.0	34.0	34.0	34.0
6	37.2827132886088	38.0	38.0	38.0	37.0	38.0
7	37.31033859637234	38.0	38.0	38.0	37.0	38.0
8	37.2396828623704	38.0	38.0	38.0	36.0	38.0
9	37.11486074405367	38.0	38.0	38.0	36.0	38.0
10-11	37.07012491247092	38.0	37.5	38.0	35.5	38.0
12-13	37.22960854735606	38.0	38.0	38.0	36.5	38.0
14-15	37.237107813241174	38.0	38.0	38.0	36.0	38.0
16-17	37.0191547514174	38.0	38.0	38.0	35.0	38.0
18-19	36.79495606604775	38.0	37.5	38.0	34.0	38.0
20-21	36.921404531182944	38.0	37.5	38.0	35.0	38.0
22-23	36.97943348919157	38.0	37.5	38.0	35.5	38.0
24-25	37.12720516816878	38.0	38.0	38.0	36.0	38.0
26-27	36.99148426735334	38.0	38.0	38.0	35.5	38.0
28-29	36.15908834225565	38.0	37.0	38.0	33.0	38.0
30-31	36.253393869576016	38.0	37.0	38.0	34.0	38.0
32-33	36.4566081633575	38.0	37.0	38.0	34.0	38.0
34-35	36.63618395789569	38.0	37.0	38.0	35.0	38.0
36-37	36.502202344649994	38.0	37.0	38.0	34.0	38.0
38-39	33.711786496803775	37.0	34.0	38.0	24.0	38.0
40-41	35.7279031420117	37.5	36.5	38.0	30.5	38.0
42-43	36.92827132886088	38.0	37.0	38.0	35.0	38.0
44-45	36.700447245375074	38.0	37.0	38.0	34.5	38.0
46-47	36.88608795825709	38.0	37.0	38.0	35.5	38.0
48-49	36.356926656276116	38.0	37.0	38.0	34.0	38.0
50-51	36.40877775518963	38.0	37.0	38.0	33.5	38.0
52-53	36.317250570350794	38.0	37.0	38.0	34.0	38.0
54-55	36.39222064105171	38.0	37.0	38.0	34.0	38.0
56-57	36.23962639199476	38.0	37.0	38.0	34.0	38.0
58-59	36.19205574755483	38.0	37.0	38.0	34.0	38.0
60-61	35.68053579092408	38.0	37.0	38.0	31.0	38.0
62-63	35.62815387047955	38.0	36.5	38.0	31.5	38.0
64-65	36.21326602064557	38.0	37.0	38.0	34.0	38.0
66-67	35.734611822637845	38.0	36.5	38.0	32.0	38.0
68-69	35.49534684104719	38.0	36.0	38.0	31.0	38.0
70-71	31.983228298434643	36.0	29.0	38.0	18.0	38.0
72-73	25.86433556956021	25.0	16.0	38.0	10.0	38.0
74-75	24.833333333333336	24.0	11.0	38.0	11.0	38.0
76	24.9255946330555	25.0	11.0	37.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	3.0
23	4.0
24	11.0
25	27.0
26	82.0
27	164.0
28	313.0
29	503.0
30	825.0
31	1239.0
32	1918.0
33	2943.0
34	5285.0
35	11562.0
36	9431.0
37	9961.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	15.514318649277852	8.622832990529576	12.340935289198292	63.52191307099429
2	7.65512412188566	8.156581057577196	66.80671319825619	17.38158162228095
3	7.600912561270358	9.909421517471934	68.43306001671523	14.056605904542478
4	5.443744211786497	55.21221567165865	26.066725395857333	13.277314720697522
5	5.479885252196698	22.007634794786654	65.81283458697568	6.699645366040976
6	11.097558220957286	66.9806419552303	13.792324546542883	8.129475277269545
7	9.744528020600393	20.388064421404533	13.625172234645705	56.24223532334936
8	9.229518194755032	66.31880915271849	8.721284813986582	15.730387838539903
9	8.804860969935172	14.991755325156422	7.871970364346864	68.33141334056154
10-11	9.821327731472069	39.23787581034989	34.84335117797204	16.097445280206006
12-13	9.814551286395156	11.273745792957014	40.27354249960471	38.638160421043125
14-15	9.973797745702605	9.493799552754625	14.567098100336565	65.96530460120621
16-17	37.18235413701972	34.56890515235707	8.831966750242822	19.416773960380386
18-19	39.09218224119627	16.431749904000363	9.114318628447517	35.361749226355855
20-21	12.036095864109688	10.258408438932936	9.232906417293488	68.4725892796639
22-23	10.082220866933207	38.05425673691582	9.271306272729326	42.59221612342166
24-25	36.936977637226114	37.928619832843914	9.135983736164444	15.998418793765529
26-27	39.159946691965395	41.856971832576626	9.22048293465248	9.762598540805493
28-29	12.21002462108378	40.77048180524497	10.285514219240586	36.733979354430666
30-31	10.072056199317837	43.23484899821553	34.72589279663889	11.967202005827742
32-33	61.35619254139279	13.362020284158929	16.298479817487745	8.98330735696054
34-35	18.520024395202277	34.84222177045922	37.71317566804455	8.924578166293962
36-37	36.228004788687855	40.27580131463034	13.560796006415035	9.935397890266765
38-39	12.084660387160895	16.42384405141063	36.78141446996905	34.71008109145942
40-41	9.26791805019087	9.967021300625692	41.044927830859926	39.720132818323506
42-43	36.62668564071288	9.470081994985431	38.52183144722279	15.3814009170789
44-45	11.662261977366674	36.4053217682004	41.3916559372953	10.540760317137629
46-47	36.15007567030336	11.515439000700232	17.652639425357457	34.68184590363895
48-49	35.18556165435612	9.031871880011746	12.14564839285311	43.63691807277902
50-51	13.763499164068502	9.20089467263115	39.663819981022094	37.37178618227826
52-53	7.926340168333051	9.357735976953059	66.32661130881772	16.389312545896175
54-55	7.636417053685436	9.461337672463474	44.58682215216335	38.31542312168774
56-57	35.149001570816715	9.436201109742454	18.162709489315056	37.25208783012578
58-59	10.589577614351397	10.710452886885598	62.70376520825567	15.996204290507338
60-61	35.07089193921934	35.889962153307344	18.602496751962942	10.436649155510365
62-63	9.714518115164319	69.60357897353052	9.592507653896993	11.089395257408182
64-65	7.212162971162643	73.01736115033151	9.052196405778767	10.718279472727067
66-67	7.403432264187182	72.36790076034029	8.96365505631941	11.26501191915312
68-69	7.346164272160477	72.28133188759375	8.944384205295021	11.428119634950756
70-71	8.265937740026205	67.44905796774047	9.542312384222654	14.742691908010663
72-73	10.391414854560859	55.87687094041232	11.92770403840723	21.8040101666196
74-75	12.204213023098209	50.0751115378099	13.245609081154347	24.47506635793754
76	11.724309304899814	51.87837440983125	12.612103824519394	23.78521246074954
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	2.5
15	4.5
16	5.0
17	4.0
18	3.0
19	3.0
20	6.0
21	8.0
22	14.5
23	29.5
24	49.0
25	60.0
26	76.0
27	92.0
28	106.0
29	120.0
30	142.5
31	185.5
32	206.5
33	207.0
34	265.5
35	343.0
36	439.0
37	516.0
38	656.5
39	3589.0
40	7824.0
41	8533.0
42	7799.0
43	6282.5
44	3763.5
45	2178.0
46	1595.0
47	1352.5
48	1016.5
49	852.0
50	781.0
51	725.5
52	630.5
53	545.0
54	499.0
55	492.0
56	484.5
57	433.5
58	383.0
59	384.5
60	371.0
61	323.0
62	290.0
63	291.0
64	275.0
65	211.5
66	189.5
67	214.0
68	186.5
69	140.5
70	110.0
71	98.0
72	98.5
73	89.5
74	73.0
75	66.0
76	52.5
77	33.5
78	28.0
79	28.0
80	28.0
81	20.5
82	14.5
83	16.0
84	9.5
85	4.0
86	2.5
87	0.0
88	0.5
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06324682071785141
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0022588150256375506
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.020329335230737954
52-53	0.030494002846106934
54-55	0.05082333807684489
56-57	0.059858598179395095
58-59	0.02371755776919428
60-61	0.030494002846106934
62-63	0.028235187820469382
64-65	0.012423482641006527
66-67	0.03275281787174448
68-69	0.0067764450769126515
70-71	0.011294075128187753
72-73	0.01919992771791918
74-75	0.007905852589731427
76	0.009035260102550203
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	44271.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.581667457251925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	55.335102087899415	10.83553567798333
2	16.47248817626024	6.451175713220844
3	11.373860883608259	6.681574845835875
4	7.232668127811744	5.665108084298977
5	3.956627061944861	3.873867768968399
6	2.145576191025493	2.5208375686115065
7	1.3265659245587726	1.818346095638228
8	0.5421617256892376	0.849314449639719
9	0.32298996424039683	0.5692213864606628
>10	1.0266466720498328	3.5124573648663913
>50	0.126888914523013	1.6850760091256127
>100	0.09228284692582767	4.2330193580447695
>500	0.011535355865728458	1.1655485532289762
>1k	0.023070711731456917	8.228863138397596
>5k	0.0	0.0
>10k+	0.011535355865728458	41.91005398567911
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT	18554	41.91005398567911	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG	2308	5.213345079171466	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC	1335	3.01551805922613	No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT	516	1.1655485532289762	RNA PCR Primer, Index 47 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC	487	1.100042917485487	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCGT	304	0.6866797677938153	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGT	292	0.6595739874861647	No Hit
CTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC	228	0.5150098258453615	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATATCGTATGCCGT	165	0.37270447923019584	No Hit
CTTTACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC	160	0.3614104041020081	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAGCTCGTATGCCGT	123	0.27783424815341873	No Hit
CTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG	115	0.2597637279483183	No Hit
CTTATACACATCTCCGAGCCCACGAGACCATGGTAGATCTCGTATGCCGT	88	0.19877572225610443	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCG	87	0.1965169072304669	No Hit
TATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCT	78	0.17618757199972895	No Hit
CCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG	75	0.1694111269228163	No Hit
CTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG	71	0.16037586682026608	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC	69	0.155858236768991	No Hit
TTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC	62	0.14004653158952812	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCAGT	57	0.12875245646134037	No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGGTAGATCTCGTATGCCGT	55	0.12423482641006527	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTCGATCTCGTATGCCGT	52	0.11745838133315263	No Hit
CTTATACACATCTCCGAGCCCACGAGAAAATGGTAGATCTCGTATGCCGT	52	0.11745838133315263	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGGTAGATCTCGTATGCCGT	49	0.11068193625623998	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATATCGTATGCCG	48	0.10842312123060242	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.022588150256375505	0.0
2	0.0	0.0	0.0	0.022588150256375505	0.0
3	0.0	0.0	0.0	0.022588150256375505	0.0
4	0.0	0.0	0.0	0.13778771656389058	0.0
5	0.0	0.0	0.0	0.16037586682026608	0.0
6	0.0	0.0	0.0	0.17618757199972895	0.0
7	0.0	0.0	0.0	0.1784463870253665	0.0
8	0.0	0.0	0.0	0.18522283210227913	0.0
9	0.0	0.0	0.0	0.1919992771791918	0.0
10	0.0	0.0	0.0	0.2100697973842922	0.0
11	0.0	0.0	0.0	0.2100697973842922	0.0
12	0.0	0.0	0.0	0.2100697973842922	0.0
13	0.0	0.0	0.0	0.21232861240992976	0.0
14	0.0	0.0	0.0	0.22588150256375505	0.0
15	0.0	0.0	0.0	0.22588150256375505	0.0
16	0.0	0.0	0.0	0.22588150256375505	0.0
17	0.0	0.0	0.0	0.22588150256375505	0.0
18	0.0	0.0	0.0	0.22588150256375505	0.0
19	0.0	0.0	0.0	0.22588150256375505	0.0
20	0.0	0.0	0.0	0.22588150256375505	0.0
21	0.0	0.0	0.0	0.2281403175893926	0.0
22	0.0	0.0	0.0	0.2281403175893926	0.0
23	0.0	0.0	0.0	0.23039913261503014	0.0
24	0.0	0.0	0.0	0.23491676266630526	0.0
25	0.0	0.0	0.0	0.23943439271758035	0.0
26	0.0	0.0	0.0	0.2416932077432179	0.0
27	0.0	0.0	0.0	0.2620225429739559	0.0
28	0.0	0.0	0.0	0.2846106932303314	0.0
29	0.0	0.0	0.0	0.311716473537982	0.0
30	0.0	0.0	0.0	0.34108106887127015	0.0
31	0.0	0.0	0.0	0.3659280341532832	0.0
32	0.0	0.0	0.0	0.3975514445122089	0.0
33	0.0	0.0	0.0	0.44272774502495993	0.0
34	0.0	0.0	0.0	0.5263039009735493	0.0
35	0.0	0.0	0.0	0.6347270222041517	0.0
36	0.0	0.0	0.0	0.7792911838449549	0.0
37	0.0	0.0	0.0	1.1023017325111246	0.0
38	0.0	0.0	0.0	1.5405118474848094	0.0
39	0.0	0.0	0.0	1.8386754308689661	0.0
40	0.0	0.0	0.0	2.3853086670732533	0.0
41	0.0	0.0	0.0	2.8167423369700257	0.0
42	0.0	0.0	0.0	3.2797994172257234	0.0
43	0.0	0.0	0.0	3.79029161301981	0.0
44	0.0	0.0	0.0	4.167513722301281	0.0
45	0.0	0.0	0.0	4.5899121320955025	0.0
46	0.0	0.0	0.0	5.014569356915362	0.0
47	0.0	0.0	0.0	5.409861986401934	0.0
48	0.0	0.0	0.0	5.811931060965417	0.0
49	0.0	0.0	0.0	6.1394592396828624	0.0
50	0.0	0.0	0.0	6.539269499220709	0.0
51	0.0	0.0	0.0	6.887127013168891	0.0
52	0.0	0.0	0.0	7.325337128142577	0.0
53	0.0	0.0	0.0	7.704818052449685	0.0
54	0.0	0.0	0.0	8.149804612500283	0.0
55	0.0	0.0	0.0	8.502179756499741	0.0
56	0.0	0.0	0.0	8.813896230037722	0.0
57	0.0	0.0	0.0	9.033001287524565	0.0
58	0.0	0.0	0.0	9.238553454857582	0.0
59	0.0	0.0	0.0	9.466693772446975	0.0
60	0.0	0.0	0.0	9.791963136138781	0.0
61	0.0	0.0	0.0	10.047209234035824	0.0
62	0.0	0.0	0.0	10.37473741275327	0.0
63	0.0	0.0	0.0	10.609654175419575	0.0
64	0.0	0.0	0.0	10.842312123060243	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATTGAA	20	7.777702E-5	70.12117	56
TCTGATT	20	7.777702E-5	70.12117	53
CAGTCTT	15	0.0022055043	70.041855	47
AGTCTTC	15	0.0022055043	70.041855	48
GTATGCA	15	0.0022154322	69.962715	42
CAATGGC	15	0.0022154322	69.962715	9
TATGCAG	15	0.0022154322	69.962715	43
CTCTCGT	15	0.0022154322	69.962715	37
TAGCTCT	15	0.0022154322	69.962715	34
ATGAATA	15	0.0022154322	69.962715	25
TTTGGTA	15	0.0022154322	69.962715	5
GTAGCTC	15	0.0022154322	69.962715	33
AATGAGT	15	0.0022154322	69.962715	31
ATATATG	15	0.0022154322	69.962715	37
GAGACAT	15	0.0022154322	69.962715	24
AGCTCTC	20	7.86554E-5	69.96271	35
CGAGACG	20	7.86554E-5	69.96271	23
GCTCTCG	20	7.86554E-5	69.96271	36
GAGACGA	20	7.86554E-5	69.96271	24
TCTTTAT	100	0.0	59.468307	1
>>END_MODULE