##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779823_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	101664
Sequences flagged as poor quality	0
Sequence length	76
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.415201054453888	32.0	21.0	34.0	12.0	34.0
2	26.870711362920996	32.0	21.0	34.0	12.0	34.0
3	26.44758223166509	32.0	21.0	34.0	11.0	34.0
4	26.93565077116777	32.0	21.0	34.0	12.0	34.0
5	27.145548079949638	34.0	21.0	34.0	11.0	34.0
6	29.40474504249292	35.0	21.0	38.0	11.0	38.0
7	29.617229304375197	36.0	21.0	38.0	12.0	38.0
8	29.029882751022978	34.0	21.0	38.0	11.0	38.0
9	29.83741540761725	36.0	23.0	38.0	11.0	38.0
10-11	28.48305201447907	34.0	21.0	38.0	11.0	38.0
12-13	29.025043279823734	34.0	21.0	38.0	11.0	38.0
14-15	27.930865399748193	33.5	16.0	38.0	11.0	38.0
16-17	28.418024079320112	34.0	21.0	38.0	11.0	38.0
18-19	29.010898646521877	35.0	21.0	38.0	11.0	38.0
20-21	28.06998544224111	32.5	16.0	38.0	11.0	38.0
22-23	28.793938857412655	35.0	21.0	38.0	11.0	38.0
24-25	29.09008596946805	35.0	21.0	38.0	11.0	38.0
26-27	28.066493547371735	34.0	21.0	38.0	11.0	38.0
28-29	28.918929021089077	35.0	21.0	38.0	11.0	38.0
30-31	29.262501967264715	36.0	21.0	38.0	11.0	38.0
32-33	29.425337385898647	36.0	21.0	38.0	11.0	38.0
34-35	29.38662161630469	36.0	21.0	38.0	11.0	38.0
36-37	28.90153348284545	35.0	21.0	38.0	11.0	38.0
38-39	28.814368901479384	35.0	21.0	38.0	11.0	38.0
40-41	28.768492288322314	34.5	21.0	38.0	11.0	38.0
42-43	29.165958451369214	35.0	21.0	38.0	11.0	38.0
44-45	29.263072474032107	36.0	21.0	38.0	11.0	38.0
46-47	29.1441070585458	36.0	21.0	38.0	11.0	38.0
48-49	28.759846159899276	34.5	21.0	38.0	11.0	38.0
50-51	28.176502990242366	34.0	16.0	38.0	11.0	38.0
52-53	28.739214471199244	34.0	21.0	38.0	11.0	38.0
54-55	28.85667984734026	35.0	21.0	38.0	11.0	38.0
56-57	28.928376809883538	35.5	21.0	38.0	11.0	38.0
58-59	29.073511764242998	36.0	21.0	38.0	11.0	38.0
60-61	29.042778171230722	35.5	21.0	38.0	11.0	38.0
62-63	28.972891092225368	36.0	21.0	38.0	11.0	38.0
64-65	28.957069365753856	35.5	21.0	38.0	11.0	38.0
66-67	28.958953021718603	35.0	21.0	38.0	11.0	38.0
68-69	28.35564703336481	34.0	21.0	38.0	11.0	38.0
70-71	28.410268138180676	34.0	21.0	38.0	11.0	38.0
72-73	28.462312126219704	34.0	21.0	38.0	11.0	38.0
74-75	28.53149590808939	34.0	21.0	38.0	11.0	38.0
76	27.671289738747245	33.0	20.0	37.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	61.0
13	593.0
14	1839.0
15	3319.0
16	4368.0
17	5199.0
18	5529.0
19	4982.0
20	4051.0
21	2775.0
22	2163.0
23	1679.0
24	1511.0
25	1402.0
26	1441.0
27	1605.0
28	1765.0
29	1833.0
30	2261.0
31	2598.0
32	3342.0
33	4054.0
34	5277.0
35	7266.0
36	11765.0
37	18983.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.920915958451374	17.673906200818383	8.516288951841359	38.88888888888889
2	19.57428391564369	21.629091910607492	31.392626691847653	27.403997481901165
3	22.17500786905886	20.19298866855524	31.061142587346552	26.570860875039344
4	14.912996862181915	26.422592290213746	27.215407768804777	31.449003078799564
5	16.093209002203338	29.752911551778404	33.315627950897074	20.83825149512118
6	25.04746072809183	39.44994737515123	15.409736088842548	20.092855807914383
7	22.475900059020262	32.95101318119221	16.898485146566987	27.67460161322054
8	20.36179065718417	36.14660489273172	15.71890892279242	27.772695527291685
9	22.033681559739517	20.065317043420094	15.952507426862617	41.94849396997777
10-11	21.323706614598446	28.941142910541355	21.52486437835365	28.210286096506543
12-13	20.88399039974819	23.176345609065155	24.466379446018255	31.473284545168394
14-15	26.236683945978378	25.38484994540787	19.801697766148944	28.57676834246481
16-17	27.180354797936328	24.30715055059092	19.280764482828154	29.2317301686446
18-19	22.059756547399484	24.5729743022255	22.50829952047215	30.858969629902866
20-21	26.347576329870947	23.543732294617563	20.785135347812403	29.323556027699087
22-23	27.14668633960408	23.2716094921923	19.768351161932866	29.81335300627075
24-25	21.993744036670172	28.302332215260222	19.22282442973353	30.48109931833607
26-27	25.245540434866005	28.012020046526036	20.52654099062102	26.215898527986937
28-29	22.313159383223077	26.982268893096915	22.423825098984334	28.28074662469567
30-31	23.429254353824135	25.81555818951738	22.559719073217035	28.19546838344145
32-33	21.414660056657222	27.06759521561221	22.689939408246772	28.82780531948379
34-35	20.72131889318421	28.508474242826647	22.512566274185776	28.25764058980337
36-37	24.764012887675545	24.53232986546644	22.759536633955584	27.944120612902434
38-39	24.901506544097032	22.965575246049195	24.75936118201984	27.373557027833932
40-41	22.162712464589234	26.746931067044383	23.14929571923198	27.941060749134405
42-43	19.358075209269842	26.261274996803163	24.666791261324178	29.713858532602817
44-45	19.127295619755856	23.23113091549365	26.910516323860673	30.731057140889824
46-47	20.384615384615383	25.06098760574464	24.98426126303364	29.570135746606336
48-49	21.047765187283602	25.80559490084986	24.314408246773684	28.832231665092856
50-51	25.234476822820607	25.075617853190707	21.952539038485185	27.737366285503505
52-53	17.261579778364737	27.475689474538505	24.466939160973304	30.79579158612345
54-55	19.294371101973358	24.73881991854723	24.012827827729357	31.953981151750057
56-57	20.242000934556454	26.218243525737194	22.601018174663682	30.93873736504267
58-59	19.34130470068024	26.667027362021727	23.617020230090844	30.374647707207185
60-61	17.72978822834936	25.355043411958576	23.896499987701993	33.01866837199006
62-63	19.119947271600445	26.307142927409917	23.166556815834262	31.406352985155383
64-65	19.673477820299663	24.873828051983825	23.228919692661858	32.22377443505465
66-67	18.2067703568161	29.98603035937392	20.934293500182	30.872905783627974
68-69	18.684588448221593	31.305083412023922	19.901341674535725	30.108986465218763
70-71	17.866382066238454	30.246008872451135	20.567463088832714	31.320145972477697
72-73	17.501389428539106	29.204558309274496	22.32430491685561	30.969747345330784
74-75	17.55534460964897	28.097994756955885	21.854050571275398	32.492610062119745
76	16.484521783609914	27.565685281185136	20.53236801463717	35.41742492056778
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	0.5
12	0.0
13	0.5
14	1.0
15	3.5
16	14.0
17	22.0
18	18.0
19	28.5
20	62.5
21	82.0
22	87.0
23	113.5
24	159.0
25	183.0
26	216.5
27	291.5
28	426.5
29	520.0
30	552.5
31	667.0
32	878.0
33	1007.0
34	1092.0
35	1379.0
36	1773.0
37	1965.0
38	2227.5
39	2669.0
40	3081.5
41	3652.0
42	3989.0
43	4125.0
44	4551.0
45	4921.5
46	5002.0
47	5138.0
48	5362.5
49	5384.5
50	5318.0
51	5305.0
52	5193.0
53	4962.0
54	4830.0
55	4686.0
56	4313.0
57	3946.0
58	3808.0
59	3544.0
60	3046.0
61	2616.0
62	2420.0
63	2269.0
64	1961.5
65	1642.5
66	1369.5
67	1259.0
68	1177.0
69	1010.5
70	864.5
71	803.0
72	725.5
73	614.0
74	576.0
75	572.0
76	530.0
77	436.5
78	397.5
79	410.0
80	356.5
81	283.5
82	245.0
83	226.0
84	199.5
85	143.5
86	103.5
87	93.0
88	83.0
89	59.5
90	35.5
91	29.5
92	34.0
93	24.5
94	13.0
95	8.5
96	6.0
97	4.5
98	3.0
99	5.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	9.836323575700347E-4
5	0.0
6	9.836323575700347E-4
7	0.003934529430280139
8	0.002950897072710104
9	0.005901794145420208
10-11	0.0024590808939250865
12-13	0.0
14-15	9.836323575700347E-4
16-17	4.918161787850173E-4
18-19	0.001475448536355052
20-21	0.0
22-23	0.001475448536355052
24-25	9.836323575700347E-4
26-27	4.918161787850173E-4
28-29	0.006393610324205225
30-31	4.918161787850173E-4
32-33	0.0
34-35	0.002950897072710104
36-37	0.016229933899905572
38-39	0.0073772426817752595
40-41	0.0
42-43	9.836323575700347E-4
44-45	0.002950897072710104
46-47	0.003934529430280139
48-49	0.0
50-51	0.001475448536355052
52-53	0.010328139754485363
54-55	0.009836323575700346
56-57	0.0113117721120554
58-59	0.008360875039345295
60-61	0.021148095687755743
62-63	0.01081995593327038
64-65	0.01672175007869059
66-67	0.014754485363550519
68-69	0.0
70-71	9.836323575700347E-4
72-73	0.0034427132514951208
74-75	0.00540997796663519
76	0.004918161787850173
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	101664.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.28737802958767
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.57986447241045	71.19137551148883
2	6.464181994191674	10.509128108278249
3	3.2151500484027102	7.8405335221907455
4	1.5767182962245885	5.12669184765502
5	0.7187802516940949	2.921388101983003
6	0.26621490803484993	1.2983947119924457
7	0.11979670861568248	0.681657223796034
8	0.03751210067763795	0.24394082467736858
9	0.01573088092933204	0.11508498583569404
>10	0.006050338818973862	0.07180516210261254
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.007869058860560278	0.0
2	0.0	0.0	0.0	0.007869058860560278	0.0
3	0.0	0.0	0.0	0.01081995593327038	0.0
4	0.0	0.0	0.0	0.011803588290840416	0.0
5	0.0	0.0	0.0	0.015738117721120555	0.0
6	0.0	0.0	0.0	0.017705382436260624	0.0
7	0.0	0.0	0.0	0.017705382436260624	0.0
8	0.0	0.0	0.0	0.017705382436260624	0.0
9	9.836323575700347E-4	0.0	0.0	0.020656279508970726	0.0
10	9.836323575700347E-4	0.0	0.0	0.022623544224110795	0.0
11	9.836323575700347E-4	0.0	0.0	0.024590808939250867	0.0
12	9.836323575700347E-4	0.0	0.0	0.0255744412968209	0.0
13	9.836323575700347E-4	0.0	0.0	0.02754170601196097	0.0
14	9.836323575700347E-4	0.0	0.0	0.030492603084671072	0.0
15	9.836323575700347E-4	0.0	0.0	0.03541076487252125	0.0
16	9.836323575700347E-4	0.0	0.0	0.04032892666037142	0.0
17	9.836323575700347E-4	0.0	0.0	0.04229619137551149	0.0
18	9.836323575700347E-4	0.0	0.0	0.04623072080579163	0.0
19	9.836323575700347E-4	0.0	0.0	0.052132514951211836	0.0
20	9.836323575700347E-4	0.0	0.0	0.05311614730878187	0.0
21	9.836323575700347E-4	0.0	0.0	0.05508341202392194	0.0
22	9.836323575700347E-4	0.0	0.0	0.059017941454202076	0.0
23	9.836323575700347E-4	0.0	0.0	0.06196883852691218	0.0
24	9.836323575700347E-4	0.0	0.0	0.06983789738747245	0.0
25	9.836323575700347E-4	0.0	0.0	0.0767233238904627	0.0
26	9.836323575700347E-4	0.0	0.0	0.0826251180358829	0.0
27	9.836323575700347E-4	0.0	0.0	0.0963959710418634	0.0
28	9.836323575700347E-4	0.0	0.0	0.12688857412653445	0.0
29	9.836323575700347E-4	0.0	0.0	0.1534466477809254	0.0
30	9.836323575700347E-4	0.0	0.0	0.20557916273213725	0.0
31	9.836323575700347E-4	0.0	0.0	0.2695152659741895	0.0
32	9.836323575700347E-4	0.0	0.0	0.340336795719232	0.0
33	9.836323575700347E-4	0.0	0.0	0.42689644318539505	0.0
34	9.836323575700347E-4	0.0	0.0	0.5626377085300598	0.0
35	9.836323575700347E-4	0.0	0.0	0.7072316650928548	0.0
36	9.836323575700347E-4	0.0	0.0	0.9039581366068619	0.0
37	9.836323575700347E-4	0.0	0.0	1.245278564683664	0.0
38	9.836323575700347E-4	0.0	0.0	1.6692241107963488	0.0
39	9.836323575700347E-4	0.0	0.0	2.187598363235757	0.0
40	9.836323575700347E-4	0.0	0.0	2.7394161158325465	0.0
41	9.836323575700347E-4	0.0	0.0	3.3128737802958765	0.0
42	9.836323575700347E-4	0.0	0.0	3.9109222536984576	0.0
43	9.836323575700347E-4	0.0	0.0	4.460772741580107	0.0
44	9.836323575700347E-4	0.0	0.0	5.012590494176896	0.0
45	9.836323575700347E-4	0.0	0.0	5.628344350015738	0.0
46	9.836323575700347E-4	0.0	0.0	6.298197985520932	0.0
47	9.836323575700347E-4	0.0	0.0	6.930673591438464	0.0
48	9.836323575700347E-4	0.0	0.0	7.592658168083097	0.0
49	9.836323575700347E-4	0.0	0.0	8.171033994334278	0.0
50	9.836323575700347E-4	0.0	0.0	8.854658482845451	0.0
51	9.836323575700347E-4	0.0	0.0	9.438936103242053	0.0
52	9.836323575700347E-4	0.0	0.0	10.048788164935473	0.0
53	9.836323575700347E-4	0.0	0.0	10.664542020774315	0.0
54	9.836323575700347E-4	0.0	0.0	11.255705067673906	0.0
55	9.836323575700347E-4	0.0	0.0	11.93047686496695	0.0
56	9.836323575700347E-4	0.0	0.0	12.565903367957192	0.0
57	9.836323575700347E-4	0.0	0.0	13.177722694365753	0.0
58	9.836323575700347E-4	0.0	0.0	13.782656594271325	0.0
59	9.836323575700347E-4	0.0	0.0	14.338408876298395	0.0
60	9.836323575700347E-4	0.0	0.0	14.865635819955934	0.0
61	9.836323575700347E-4	0.0	0.0	15.313188542650298	0.0
62	9.836323575700347E-4	0.0	0.0	15.807955618508027	0.0
63	9.836323575700347E-4	0.0	0.0	16.321411709159584	0.0
64	9.836323575700347E-4	0.0	0.0	16.7738825936418	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATACG	20	7.9079684E-5	69.9749	25
CATGACG	20	7.9272795E-5	69.94048	11
TTGTCGA	15	0.002226557	69.94048	39
CTCGGTG	120	0.0	61.258186	44
TCGGTGG	110	0.0	60.462624	45
TCCGACT	30	8.343179E-6	58.31242	20
GATCTCG	145	0.0	57.938774	41
GTAGATC	185	0.0	56.708504	38
GATACGC	25	2.3848627E-4	55.979923	26
GTCCATA	25	2.3848627E-4	55.979923	22
CGGTGGT	70	0.0	55.00735	46
TCTCGGT	115	0.0	54.789932	43
GTGTAGA	220	0.0	54.071514	36
AGATCTC	210	0.0	53.314213	40
AAACTGG	20	0.006930229	52.507015	70
CTCGTCG	20	0.006930229	52.507015	44
ACCGCTA	20	0.006930229	52.507015	42
TCGATCA	20	0.006930229	52.507015	42
CGGTCGA	20	0.006930229	52.507015	49
GCGTGGA	20	0.006930229	52.507015	51
>>END_MODULE