FastQCFastQC Report
Wed 25 May 2016
SRR1779823_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779823_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101664
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT2457724.17473245199874No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG30863.0354894554611267No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC17461.7174220963172806No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT6590.6482137236386528Illumina PCR Primer Index 9 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC6260.6157538558388417No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGT4790.47115989927604657No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT4430.4357491344035253No Hit
CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC2480.24394082467736858No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT2100.2065627950897073No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAGCTCGTATGCCGT1820.1790210890777463No Hit
CTTTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC1790.1760701920050362No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG1540.15147938306578532No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCG1410.1386921624173749No Hit
TTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC1060.10426502990242367No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC1020.10033050047214354No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTATG150.00220416570.11883550
CTTATGC150.00220416570.11883551
CGTCTTA150.002208481870.0842848
AAACGCA150.00221280570.0497669
TAACCGA150.002225811669.9463931
GCTTATA150.002225811669.946393
TTAACCG150.002225811669.9463930
CGAGAAG150.002225811669.9463923
GTTAACT207.9239675E-569.946383
TATGCAG308.363388E-658.28865443
GTATGCA308.363388E-658.28865442
TCTTTAT1150.054.767591
ATCTCGT37400.053.1143737
TCTCGTA37250.053.04658538
GATCTCG37450.052.9500736
CGTATGC39600.052.7247441
TGCCGTC39150.052.71984545
CTCGTAT39150.052.70544439
ATGCCGT39150.052.70544444
AGATCTC37650.052.66879335