##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779823_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	101664
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.484379918161785	34.0	34.0	34.0	34.0	34.0
2	33.58187755744413	34.0	34.0	34.0	34.0	34.0
3	33.73127163991187	34.0	34.0	34.0	34.0	34.0
4	33.491501416430594	34.0	34.0	34.0	34.0	34.0
5	33.56445742839156	34.0	34.0	34.0	34.0	34.0
6	37.20728084671073	38.0	38.0	38.0	36.0	38.0
7	37.27389242996538	38.0	38.0	38.0	36.0	38.0
8	37.15997796663519	38.0	38.0	38.0	36.0	38.0
9	37.06810670443815	38.0	38.0	38.0	36.0	38.0
10-11	37.01676601353478	38.0	37.5	38.0	35.5	38.0
12-13	37.20819070664149	38.0	38.0	38.0	36.0	38.0
14-15	37.233538912496066	38.0	38.0	38.0	36.0	38.0
16-17	37.082084120239216	38.0	38.0	38.0	36.0	38.0
18-19	36.91549614416116	38.0	37.5	38.0	34.5	38.0
20-21	36.962769515265975	38.0	37.5	38.0	35.0	38.0
22-23	36.98127655807366	38.0	37.5	38.0	35.5	38.0
24-25	37.12340159741895	38.0	38.0	38.0	36.0	38.0
26-27	36.96933034309097	38.0	38.0	38.0	35.5	38.0
28-29	36.37172942241108	38.0	37.0	38.0	34.0	38.0
30-31	36.8067113235757	38.0	37.0	38.0	35.0	38.0
32-33	36.77154154863078	38.0	37.0	38.0	35.0	38.0
34-35	36.830210300598054	38.0	37.0	38.0	35.0	38.0
36-37	36.77609576644633	38.0	37.0	38.0	34.5	38.0
38-39	35.074052762039656	38.0	35.5	38.0	28.5	38.0
40-41	36.222295994649045	38.0	37.0	38.0	33.0	38.0
42-43	36.973987842304055	38.0	37.5	38.0	35.5	38.0
44-45	36.71861720963173	38.0	37.0	38.0	34.5	38.0
46-47	36.89715140069248	38.0	37.5	38.0	35.5	38.0
48-49	36.450365911237014	38.0	37.0	38.0	34.0	38.0
50-51	36.56325247875354	38.0	37.0	38.0	34.0	38.0
52-53	36.461028485993076	38.0	37.0	38.0	34.0	38.0
54-55	36.50221317280453	38.0	37.0	38.0	34.0	38.0
56-57	36.33469566414857	38.0	37.0	38.0	34.0	38.0
58-59	36.37996242524394	38.0	37.0	38.0	34.0	38.0
60-61	35.95274138338055	38.0	37.0	38.0	32.5	38.0
62-63	35.795891367642426	38.0	37.0	38.0	31.5	38.0
64-65	36.312190155807365	38.0	37.0	38.0	34.0	38.0
66-67	35.97144515265974	38.0	37.0	38.0	32.5	38.0
68-69	35.917950306893296	38.0	37.0	38.0	31.0	38.0
70-71	33.941773882593644	37.5	34.0	38.0	21.0	38.0
72-73	30.43994432640856	37.0	23.0	38.0	11.0	38.0
74-75	29.933511370790054	37.0	23.0	38.0	11.0	38.0
76	29.797509442870634	37.0	23.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	2.0
22	5.0
23	19.0
24	39.0
25	91.0
26	163.0
27	295.0
28	539.0
29	840.0
30	1459.0
31	2238.0
32	3250.0
33	5294.0
34	8826.0
35	18685.0
36	19829.0
37	40088.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.38949025782326	11.11198582956111	9.801220232237748	47.69730368037788
2	16.75716084356311	13.753147623544223	48.52553509600252	20.964156436890146
3	16.670601196096946	14.435788479697829	49.6094979540447	19.28411237016053
4	12.320978910922253	38.78068932955618	26.675125904941773	22.223205854579792
5	12.709513692162416	25.34722222222222	49.50129839471199	12.441965690903368
6	20.868744098205855	50.679689959080896	15.938778722064843	12.512787220648411
7	19.368704752911555	23.457664463330186	16.113865281712307	41.059765502045956
8	17.779154863078375	49.34785174693106	14.054139124960654	18.818854265029902
9	16.935198300283286	16.324362606232295	12.670168397859616	54.0702706956248
10-11	18.327038086244883	32.40281712307208	29.447493704752915	19.822651085930122
12-13	17.825385583884167	15.670738904627008	33.0751298394712	33.42874567201763
14-15	17.25586244885112	14.692516525023608	17.586854737173436	50.46476628895184
16-17	33.202018413597735	29.74405886056028	14.398410450110166	22.655512275731823
18-19	33.954005350960024	19.132141170915958	15.30531948378974	31.608533994334277
20-21	18.92262747875354	15.88664620711363	14.81940509915014	50.371321214982686
22-23	17.451605288007556	31.627714825306892	15.137610166824048	35.7830697198615
24-25	32.944463024532276	31.23340087741732	14.97708091518955	20.845055182860854
26-27	34.269751337740004	33.06922804532578	15.565490242367014	17.095530374567204
28-29	31.693618193264083	19.59198929807995	16.16796506137866	32.5464274472773
30-31	20.210694050991503	16.498957349700976	44.62199008498583	18.668358514321685
32-33	16.6622403210576	45.47775023607177	20.7162810827825	17.143728360088133
34-35	16.561418004406672	34.49254406672962	16.308624488511175	32.63741344035254
36-37	32.036905886056026	33.08890069247718	16.18173591438464	18.692457507082153
38-39	18.136213408876298	19.12574756059175	30.973107491344038	31.764931539187913
40-41	16.583549732452	15.518767705382436	33.68694916587976	34.210733396285804
42-43	32.21346789423985	15.07514951211835	32.62019987409506	20.091182719546744
44-45	17.33061850802644	30.31850015738118	34.375	17.975881334592383
46-47	31.011469153289266	16.410430437519672	21.29564054139125	31.282459867799812
48-49	30.172922568460812	14.799240635819954	18.271954674220964	36.75588212149827
50-51	17.71291404735905	15.250027053881492	33.80407087133174	33.232988027427716
52-53	14.514050139714275	15.175725136762564	49.442126805462635	20.86809791806053
54-55	14.246560513315096	15.175566358966282	36.837443659338284	33.74042946838034
56-57	29.775623677606657	15.18673424199183	21.652807164296608	33.38483491610491
58-59	15.535580745463848	15.810111386625733	47.04116975636636	21.61313811154406
60-61	29.514180995252502	30.337736452414337	21.90047474971097	18.24760780262219
62-63	15.08683032419934	49.44507305554189	16.785064200324694	18.68303241993408
64-65	13.577338147189893	51.01031962302388	16.3284178217627	19.08392440802353
66-67	13.674469404020426	51.3849120839114	15.51150731568124	19.429111196386927
68-69	13.853229059072353	51.46033151345236	15.502434705621956	19.184004721853327
70-71	14.563340793389242	48.63185853766508	15.7595730552618	21.045227613683874
72-73	15.361949549407736	41.85293770414387	17.24105702254929	25.5440557238991
74-75	16.163399818007427	38.760974889943675	18.26024937901183	26.815375913037066
76	15.827996340347667	39.90693464766009	17.6096174089268	26.65545160306545
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	7.0
16	12.5
17	12.0
18	28.5
19	40.0
20	56.0
21	77.0
22	90.5
23	112.0
24	139.5
25	159.0
26	185.0
27	263.5
28	373.0
29	430.0
30	498.5
31	642.0
32	756.0
33	795.0
34	950.5
35	1231.5
36	1428.5
37	1500.0
38	1750.0
39	5816.5
40	11564.0
41	12384.0
42	11273.0
43	9370.5
44	6157.5
45	4204.0
46	3561.0
47	3296.5
48	2976.0
49	2892.0
50	2864.0
51	2771.0
52	2824.5
53	2864.5
54	2758.0
55	2759.0
56	2627.5
57	2494.0
58	2493.0
59	2276.5
60	1954.5
61	1703.5
62	1558.0
63	1473.0
64	1323.0
65	1198.0
66	1083.5
67	1029.0
68	963.5
69	834.5
70	735.5
71	700.0
72	664.5
73	595.5
74	578.0
75	594.0
76	498.5
77	413.5
78	361.0
79	298.0
80	301.0
81	283.0
82	234.0
83	206.0
84	193.0
85	143.0
86	100.5
87	95.0
88	76.5
89	53.0
90	28.5
91	10.0
92	11.0
93	8.5
94	5.5
95	3.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04327982373308152
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0019672647151400694
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.014754485363550519
52-53	0.027541706011960966
54-55	0.049181617878501734
56-57	0.04819798552093169
58-59	0.03541076487252125
60-61	0.03098441926345609
62-63	0.028525338369531004
64-65	0.01278722064841045
66-67	0.032459867799811144
68-69	0.008852691218130312
70-71	0.0113117721120554
72-73	0.019672647151400692
74-75	0.0113117721120554
76	0.014754485363550519
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	101664.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.134482215926976
#Duplication Level	Percentage of deduplicated	Percentage of total
1	44.04408033060248	11.95113314447592
2	18.46951352135141	10.023213723638653
3	14.191981439860799	11.552762039660056
4	9.860073950554629	10.701920050361977
5	5.981294859711448	8.114966949952786
6	3.7047777858333935	6.031633616619452
7	1.95751468136011	3.7181303116147304
8	0.8808816066120495	1.9121813031161474
9	0.3625027187703908	0.8852691218130312
>10	0.4531283984629885	1.9407066414856782
>50	0.039875299064742985	0.7692005036197671
>100	0.036250271877039074	2.207271010387158
>500	0.007250054375407816	1.2639675794774945
>1k	0.007250054375407816	4.752911551778408
>5k	0.0	0.0
>10k+	0.003625027187703908	24.17473245199874
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT	24577	24.17473245199874	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG	3086	3.0354894554611267	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC	1746	1.7174220963172806	No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT	659	0.6482137236386528	Illumina PCR Primer Index 9 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC	626	0.6157538558388417	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGT	479	0.47115989927604657	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT	443	0.4357491344035253	No Hit
CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC	248	0.24394082467736858	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT	210	0.2065627950897073	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAGCTCGTATGCCGT	182	0.1790210890777463	No Hit
CTTTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC	179	0.1760701920050362	No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG	154	0.15147938306578532	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCG	141	0.1386921624173749	No Hit
TTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC	106	0.10426502990242367	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC	102	0.10033050047214354	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009836323575700346	0.0
2	0.0	0.0	0.0	0.009836323575700346	0.0
3	0.0	0.0	0.0	0.013770853005980485	0.0
4	0.0	0.0	0.0	0.014754485363550519	0.0
5	0.0	0.0	0.0	0.019672647151400692	0.0
6	0.0	0.0	0.0	0.022623544224110795	0.0
7	0.0	0.0	0.0	0.023607176581680833	0.0
8	0.0	0.0	0.0	0.023607176581680833	0.0
9	0.0	0.0	0.0	0.024590808939250867	0.0
10	0.0	0.0	0.0	0.02754170601196097	0.0
11	0.0	0.0	0.0	0.030492603084671072	0.0
12	0.0	0.0	0.0	0.03344350015738118	0.0
13	0.0	0.0	0.0	0.03639439723009128	0.0
14	0.0	0.0	0.0	0.04032892666037142	0.0
15	0.0	0.0	0.0	0.0481979855209317	0.0
16	0.0	0.0	0.0	0.05508341202392194	0.0
17	0.0	0.0	0.0	0.05705067673906201	0.0
18	0.0	0.0	0.0	0.06491973559962229	0.0
19	0.0	0.0	0.0	0.07377242681775259	0.0
20	0.0	0.0	0.0	0.07573969153289267	0.0
21	0.0	0.0	0.0	0.07869058860560277	0.0
22	0.0	0.0	0.0	0.07967422096317281	0.0
23	0.0	0.0	0.0	0.08164148567831288	0.0
24	0.0	0.0	0.0	0.08754327982373308	0.0
25	0.0	0.0	0.0	0.0963959710418634	0.0
26	0.0	0.0	0.0	0.10426502990242367	0.0
27	0.0	0.0	0.0	0.12197041233868429	0.0
28	0.0	0.0	0.0	0.16033207428391563	0.0
29	0.0	0.0	0.0	0.18787378029587662	0.0
30	0.0	0.0	0.0	0.24787535410764872	0.0
31	0.0	0.0	0.0	0.32066414856783126	0.0
32	0.0	0.0	0.0	0.4023056342461442	0.0
33	0.0	0.0	0.0	0.5036197670758578	0.0
34	0.0	0.0	0.0	0.6590336795719232	0.0
35	0.0	0.0	0.0	0.8272348127163991	0.0
36	0.0	0.0	0.0	1.054453887315077	0.0
37	0.0	0.0	0.0	1.4351196096946806	0.0
38	0.0	0.0	0.0	1.902344979540447	0.0
39	0.0	0.0	0.0	2.449244570349386	0.0
40	0.0	0.0	0.0	3.070900220333648	0.0
41	0.0	0.0	0.0	3.6718995908089394	0.0
42	0.0	0.0	0.0	4.339785961598992	0.0
43	0.0	0.0	0.0	4.927014479068303	0.0
44	0.0	0.0	0.0	5.527030217186025	0.0
45	0.0	0.0	0.0	6.1909820585457975	0.0
46	0.0	0.0	0.0	6.921820900220333	0.0
47	0.0	0.0	0.0	7.6300361976707585	0.0
48	0.0	0.0	0.0	8.359891406987725	0.0
49	0.0	0.0	0.0	8.993350645262826	0.0
50	0.0	0.0	0.0	9.726156751652502	0.0
51	0.0	0.0	0.0	10.328139754485363	0.0
52	0.0	0.0	0.0	10.933073654390935	0.0
53	0.0	0.0	0.0	11.549811142587346	0.0
54	0.0	0.0	0.0	12.175401322001889	0.0
55	0.0	0.0	0.0	12.855091281082782	0.0
56	0.0	0.0	0.0	13.504288637079005	0.0
57	0.0	0.0	0.0	14.141682404784389	0.0
58	0.0	0.0	0.0	14.766288951841359	0.0
59	0.0	0.0	0.0	15.34958293988039	0.0
60	0.0	0.0	0.0	15.90828611898017	0.0
61	0.0	0.0	0.0	16.389282341831915	0.0
62	0.0	0.0	0.0	16.924378344350014	0.0
63	0.0	0.0	0.0	17.471277935158955	0.0
64	0.0	0.0	0.0	17.968995908089394	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTATG	15	0.002204165	70.118835	50
CTTATGC	15	0.002204165	70.118835	51
CGTCTTA	15	0.0022084818	70.08428	48
AAACGCA	15	0.002212805	70.04976	69
TAACCGA	15	0.0022258116	69.94639	31
GCTTATA	15	0.0022258116	69.94639	3
TTAACCG	15	0.0022258116	69.94639	30
CGAGAAG	15	0.0022258116	69.94639	23
GTTAACT	20	7.9239675E-5	69.94638	3
TATGCAG	30	8.363388E-6	58.288654	43
GTATGCA	30	8.363388E-6	58.288654	42
TCTTTAT	115	0.0	54.76759	1
ATCTCGT	3740	0.0	53.11437	37
TCTCGTA	3725	0.0	53.046585	38
GATCTCG	3745	0.0	52.95007	36
CGTATGC	3960	0.0	52.72474	41
TGCCGTC	3915	0.0	52.719845	45
CTCGTAT	3915	0.0	52.705444	39
ATGCCGT	3915	0.0	52.705444	44
AGATCTC	3765	0.0	52.668793	35
>>END_MODULE