SRR1779822_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1779822_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	49862
Sequences flagged as poor quality	0
Sequence length	76
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT	24226	48.586097629457306	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG	3108	6.233203642052064	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC	1751	3.5116922706670413	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC	600	1.2033211664193173	No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT	578	1.1591993903172757	RNA PCR Primer, Index 42 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT	485	0.9726846095222815	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGT	424	0.8503469576029843	No Hit
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC	266	0.5334723837792307	RNA PCR Primer, Index 32 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT	264	0.5294613132244996	No Hit
CTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG	195	0.39107937908627816	No Hit
CTTTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC	166	0.3329188560426778	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAGCTCGTATGCCGT	160	0.3208856443784846	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCG	126	0.2526974449480566	No Hit
CCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG	125	0.2506919096706911	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC	103	0.20657013356864948	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCCCTCGATCTCGTATGCCGT	101	0.2025590630139184	No Hit
TTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC	89	0.17849263968553208	RNA PCR Primer, Index 32 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG	85	0.17047049857606994	No Hit
TATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCT	80	0.1604428221892423	RNA PCR Primer, Index 42 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCG	75	0.15041514580241466	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCG	71	0.14239300469295255	No Hit
CTTATACACATCTCCGAGCCCACGAGACATAACTCGATCTCGTATGCCGT	70	0.14038746941558702	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCC	67	0.13437086358349043	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCAGT	65	0.13035979302875939	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTCTGCCGT	64	0.12835425775139386	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACCCGATCTCGTATGCCGT	60	0.12033211664193173	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTACTCGATCTCGTATGCCGT	54	0.10829890497773857	No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGC	53	0.10629336970037304	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCACTCGATCTCGTATGCCGT	53	0.10629336970037304	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCC	52	0.10428783442300749	No Hit
CTTATACACCTCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT	50	0.10027676386827643	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAACTC	15	0.0022153002	69.97694	29
AACTCGA	15	0.0022153002	69.97694	31
AACCACC	15	0.0022153002	69.97694	70
ATAGCTT	15	0.0022153002	69.97694	3
TAACTCG	15	0.0022153002	69.97694	30
TACAAGT	15	0.0022153002	69.97694	7
ACATAAC	15	0.0022153002	69.97694	27
TTTACAC	15	0.0022153002	69.97694	2
CATACAA	20	7.8674006E-5	69.97694	5
GAGACTT	15	0.0022153002	69.97694	24
TTGACAA	40	1.02591E-8	61.35289	58
CTTGACA	40	1.02591E-8	61.35289	57
TGAACAA	40	1.0340955E-8	61.29129	59
GAACAAA	35	2.924844E-7	59.980225	60
AACAAAG	30	8.279743E-6	58.31411	69
CTATACA	30	8.279743E-6	58.31411	1
CCTTATA	30	8.279743E-6	58.31411	1
ACAAAGA	25	2.3727011E-4	55.981544	70
ACCCGAT	25	2.3727011E-4	55.981544	32
GGCCCTG	25	2.3727011E-4	55.981544	7

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content