##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779822_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	49862
Sequences flagged as poor quality	0
Sequence length	76
%GC	44
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.49947856082788	34.0	34.0	34.0	34.0	34.0
2	33.59221451205327	34.0	34.0	34.0	34.0	34.0
3	33.73905980506197	34.0	34.0	34.0	34.0	34.0
4	33.491215755485136	34.0	34.0	34.0	34.0	34.0
5	33.607055473105774	34.0	34.0	34.0	34.0	34.0
6	37.31155990533873	38.0	38.0	38.0	37.0	38.0
7	37.32690225021058	38.0	38.0	38.0	37.0	38.0
8	37.26653563836188	38.0	38.0	38.0	36.0	38.0
9	37.12799326140147	38.0	38.0	38.0	36.0	38.0
10-11	37.0931771689864	38.0	37.5	38.0	35.5	38.0
12-13	37.271760057759415	38.0	38.0	38.0	36.5	38.0
14-15	37.28746339898119	38.0	38.0	38.0	36.5	38.0
16-17	37.05630540291203	38.0	38.0	38.0	35.5	38.0
18-19	36.81105852151939	38.0	37.5	38.0	34.0	38.0
20-21	36.90756487906623	38.0	37.5	38.0	35.0	38.0
22-23	37.058992820183704	38.0	38.0	38.0	36.0	38.0
24-25	37.1278127632265	38.0	38.0	38.0	36.0	38.0
26-27	36.94537924672095	38.0	37.5	38.0	35.5	38.0
28-29	36.074234888291684	38.0	37.0	38.0	32.5	38.0
30-31	36.27236171834262	38.0	37.0	38.0	33.0	38.0
32-33	36.200994745497574	38.0	37.0	38.0	34.0	38.0
34-35	36.10029681922105	38.0	37.0	38.0	33.5	38.0
36-37	36.39437848461755	38.0	37.0	38.0	34.0	38.0
38-39	32.814818900164454	36.5	32.5	38.0	16.5	38.0
40-41	35.35006618266415	37.5	35.5	38.0	30.5	38.0
42-43	36.883498455737836	38.0	37.0	38.0	35.0	38.0
44-45	36.64992378965946	38.0	37.0	38.0	34.5	38.0
46-47	36.87608800288797	38.0	37.0	38.0	35.5	38.0
48-49	36.32788496249649	38.0	37.0	38.0	34.0	38.0
50-51	36.357306165015444	38.0	37.0	38.0	33.5	38.0
52-53	36.25159440054551	38.0	37.0	38.0	34.0	38.0
54-55	36.33619790622117	38.0	37.0	38.0	34.0	38.0
56-57	36.1747122056877	38.0	37.0	38.0	33.5	38.0
58-59	36.10848943082909	38.0	37.0	38.0	34.0	38.0
60-61	35.56089807869721	38.0	36.5	38.0	30.5	38.0
62-63	35.55066984878264	38.0	36.5	38.0	31.0	38.0
64-65	36.18454935622317	38.0	37.0	38.0	34.0	38.0
66-67	35.66395250892463	38.0	36.5	38.0	31.5	38.0
68-69	35.36749428422446	38.0	36.0	38.0	31.0	38.0
70-71	31.316363162328024	34.5	28.0	38.0	17.5	38.0
72-73	24.126830050940598	23.0	11.0	35.5	10.0	38.0
74-75	23.00433195619911	23.0	11.0	34.0	10.5	38.0
76	23.070815450643778	23.0	11.0	34.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	2.0
23	6.0
24	18.0
25	39.0
26	102.0
27	180.0
28	360.0
29	655.0
30	988.0
31	1507.0
32	2345.0
33	3793.0
34	6676.0
35	14680.0
36	10486.0
37	8025.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.062062323173546	6.778096594899371	12.777655155807935	69.38218592611915
2	5.485138983594721	5.842124262965786	72.45998957121655	16.212747182222937
3	5.876218362681	7.66114475953632	74.5517628655088	11.910874012273876
4	4.22566282940917	61.02442741967832	24.712205687698045	10.037704063214473
5	4.2457181821828245	20.033291885604267	70.85355581404677	4.8674341181661385
6	8.046207532790502	71.30079018089928	13.623601139144037	7.029401147166178
7	7.528779431230196	18.914203200834304	11.804580642573503	61.752436725362
8	6.455818057839638	71.03405398900968	7.077533993822952	15.432593959327745
9	6.367574505635554	14.546147366732182	6.158998836789539	72.92727929084273
10-11	7.178813525329911	41.92872327624243	36.085596245637966	14.806866952789699
12-13	7.5468292487264845	8.82535798804701	42.79411174842566	40.83370101480085
14-15	7.520757290120733	7.107617022983434	13.468172155148208	71.90345353174762
16-17	39.38871284745898	35.505996550479324	6.480887248806706	18.624403353254984
18-19	42.02799727247202	14.782800529461312	6.362560667442141	36.82664153062452
20-21	9.918374714211224	7.768440896875376	6.726565320283983	75.58661906862942
22-23	7.652119850788175	39.91316032249007	6.434759937427299	45.99995988929445
24-25	39.660860583817175	38.9669384194218	6.335549472037544	15.036651524723485
26-27	41.772291524607915	43.613372909229476	7.104608720067386	7.509726846095223
28-29	9.398941077373552	43.57326220368216	7.355300629738077	39.67249608920621
30-31	6.472865107697244	15.072600377040631	39.891099434439056	38.56343508082307
32-33	7.1918495046327875	38.91239821908468	10.8098351450002	43.08591713128234
34-35	7.012354097308572	11.091612851470058	39.52809754923589	42.367935501985485
36-37	38.48120813445108	35.6122899201797	11.180859171312823	14.725642774056396
38-39	10.376639525089246	14.773775620713167	38.280654606714535	36.56893024748305
40-41	6.972243391761261	7.184830131162006	43.234326741807386	42.608599735269344
42-43	39.19356617831393	6.691535553483148	39.93762722741995	14.17727104078297
44-45	9.589466928723276	38.396975652801736	43.722674581845894	8.290882836629097
46-47	38.903373310336534	9.455096065139786	15.961052504913562	35.68047811961013
48-49	37.44534916369179	6.29738077092776	9.14423809714813	47.11303196823232
50-51	12.87973763125959	6.362642542649964	41.20071810404485	39.556901722045595
52-53	6.12072943206211	6.42265331915662	72.28519268963028	15.171424559151003
54-55	5.804924052410859	6.552360695868531	47.47777755482874	40.16493769689187
56-57	38.095954731519384	6.416044626374508	16.485071032988202	39.002929609117906
58-59	9.275123889011496	7.671087213851494	68.38372489617399	14.670064000963023
60-61	37.55253944847372	36.769087243070814	17.59005687802823	8.088316430427238
62-63	8.427619964692665	76.29995185363505	7.194872412132884	8.0775557695394
64-65	5.315627946161715	80.42244197941949	6.4830601969791175	7.778869877439673
66-67	5.1620022068412075	80.37917544387602	6.337646704784833	8.121175644497944
68-69	5.299102441959585	80.00902572331144	6.254826254826256	8.437045579902723
70-71	5.995266367137356	74.58981867779204	7.375240693196405	12.039674261874197
72-73	8.280088668666059	59.92758056912445	9.873917971453503	21.91841279075599
74-75	9.948851669842544	53.47106609166583	11.5194062782068	25.06067596028483
76	9.24718672898321	55.268489358714625	10.581108458869075	24.903215453433095
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.5
17	1.0
18	4.0
19	6.5
20	11.0
21	16.0
22	19.0
23	25.5
24	45.0
25	61.0
26	72.5
27	88.0
28	95.5
29	99.0
30	142.0
31	216.5
32	254.5
33	261.0
34	274.5
35	304.0
36	361.5
37	403.0
38	412.5
39	618.5
40	4470.0
41	9931.5
42	11738.0
43	10717.5
44	7682.0
45	4230.0
46	2793.0
47	2086.5
48	1133.5
49	718.5
50	550.0
51	529.0
52	524.5
53	499.0
54	457.0
55	496.0
56	500.0
57	472.5
58	480.0
59	429.5
60	357.5
61	351.0
62	366.0
63	339.5
64	273.0
65	213.5
66	185.0
67	176.0
68	159.0
69	128.5
70	110.0
71	105.0
72	95.5
73	75.0
74	60.0
75	56.0
76	44.0
77	35.5
78	25.0
79	11.0
80	11.5
81	11.0
82	5.5
83	1.0
84	2.0
85	3.0
86	2.5
87	2.0
88	1.0
89	0.0
90	1.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.050138381934138214
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0010027676386827646
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0010027676386827646
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.017047049857606994
52-53	0.030083029160482933
54-55	0.050138381934138214
56-57	0.05214391721150374
58-59	0.038105170269945046
60-61	0.037102402631262285
62-63	0.028077493883117408
64-65	0.01804981749628976
66-67	0.03409409971521399
68-69	0.00902490874814488
70-71	0.012033211664193173
72-73	0.027074726244434643
74-75	0.014038746941558704
76	0.01804981749628976
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	49862.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.20119529902531
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.506001977121876	8.166539649432433
2	13.61389634232453	3.866672014760739
3	9.306595113684509	3.9649432433516507
4	6.948171162265217	3.9468934258553605
5	4.716847902838582	3.349243913200433
6	3.234006496257591	2.7556054711002367
7	1.7370427905663042	1.7267658738117204
8	0.7626041519559384	0.8663912398219086
9	0.3954243750882644	0.5053948898961133
>10	1.355740714588335	3.4334763948497855
>50	0.1977121875441322	1.8811920901688661
>100	0.15534529021324672	4.843367694837752
>500	0.028244598220590313	2.362520556736593
>1k	0.028244598220590313	9.744895912719105
>5k	0.0	0.0
>10k+	0.014122299110295156	48.586097629457306
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT	24226	48.586097629457306	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG	3108	6.233203642052064	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC	1751	3.5116922706670413	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC	600	1.2033211664193173	No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT	578	1.1591993903172757	RNA PCR Primer, Index 42 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT	485	0.9726846095222815	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGT	424	0.8503469576029843	No Hit
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC	266	0.5334723837792307	RNA PCR Primer, Index 32 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT	264	0.5294613132244996	No Hit
CTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG	195	0.39107937908627816	No Hit
CTTTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC	166	0.3329188560426778	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAGCTCGTATGCCGT	160	0.3208856443784846	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCG	126	0.2526974449480566	No Hit
CCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG	125	0.2506919096706911	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC	103	0.20657013356864948	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCCCTCGATCTCGTATGCCGT	101	0.2025590630139184	No Hit
TTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC	89	0.17849263968553208	RNA PCR Primer, Index 32 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG	85	0.17047049857606994	No Hit
TATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCT	80	0.1604428221892423	RNA PCR Primer, Index 42 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCG	75	0.15041514580241466	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCG	71	0.14239300469295255	No Hit
CTTATACACATCTCCGAGCCCACGAGACATAACTCGATCTCGTATGCCGT	70	0.14038746941558702	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCC	67	0.13437086358349043	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCAGT	65	0.13035979302875939	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTCTGCCGT	64	0.12835425775139386	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACCCGATCTCGTATGCCGT	60	0.12033211664193173	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTACTCGATCTCGTATGCCGT	54	0.10829890497773857	No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGC	53	0.10629336970037304	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCACTCGATCTCGTATGCCGT	53	0.10629336970037304	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCC	52	0.10428783442300749	No Hit
CTTATACACCTCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT	50	0.10027676386827643	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.01604428221892423	0.0
6	0.0	0.0	0.0	0.01604428221892423	0.0
7	0.0	0.0	0.0	0.01604428221892423	0.0
8	0.0	0.0	0.0	0.01604428221892423	0.0
9	0.0	0.0	0.0	0.01604428221892423	0.0
10	0.0	0.0	0.0	0.01804981749628976	0.0
11	0.0	0.0	0.0	0.028077493883117404	0.0
12	0.0	0.0	0.0	0.030083029160482933	0.0
13	0.0	0.0	0.0	0.03208856443784846	0.0
14	0.0	0.0	0.0	0.03208856443784846	0.0
15	0.0	0.0	0.0	0.038105170269945046	0.0
16	0.0	0.0	0.0	0.038105170269945046	0.0
17	0.0	0.0	0.0	0.038105170269945046	0.0
18	0.0	0.0	0.0	0.04612731137940716	0.0
19	0.0	0.0	0.0	0.05013838193413822	0.0
20	0.0	0.0	0.0	0.062171593598331396	0.0
21	0.0	0.0	0.0	0.062171593598331396	0.0
22	0.0	0.0	0.0	0.062171593598331396	0.0
23	0.0	0.0	0.0	0.10228229914564198	0.0
24	0.0	0.0	0.0	0.1042878344230075	0.0
25	0.0	0.0	0.0	0.1183265813645662	0.0
26	0.0	0.0	0.0	0.1183265813645662	0.0
27	0.0	0.0	0.0	0.12835425775139384	0.0
28	0.0	0.0	0.0	0.1383819341382215	0.0
29	0.0	0.0	0.0	0.1664594280213389	0.0
30	0.0	0.0	0.0	0.18852031607235972	0.0
31	0.0	0.0	0.0	0.22863102161967028	0.0
32	0.0	0.0	0.0	0.242669768561229	0.0
33	0.0	0.0	0.0	0.28077493883117405	0.0
34	0.0	0.0	0.0	0.33091332076531227	0.0
35	0.0	0.0	0.0	0.4392122257430508	0.0
36	0.0	0.0	0.0	0.5715775540491758	0.0
37	0.0	0.0	0.0	0.8623801692671774	0.0
38	0.0	0.0	0.0	1.0829890497773855	0.0
39	0.0	0.0	0.0	1.3858248766595804	0.0
40	0.0	0.0	0.0	1.6505555332718302	0.0
41	0.0	0.0	0.0	1.9092695840519835	0.0
42	0.0	0.0	0.0	2.16196702900004	0.0
43	0.0	0.0	0.0	2.4567807147727727	0.0
44	0.0	0.0	0.0	2.881954193574265	0.0
45	0.0	0.0	0.0	3.158718061850708	0.0
46	0.0	0.0	0.0	3.515703341221772	0.0
47	0.0	0.0	0.0	3.7663952508924634	0.0
48	0.0	0.0	0.0	4.021098231117885	0.0
49	0.0	0.0	0.0	4.295856564116963	0.0
50	0.0	0.0	0.0	4.542537403232923	0.0
51	0.0	0.0	0.0	4.7952348481809794	0.0
52	0.0	0.0	0.0	5.120131563114195	0.0
53	0.0	0.0	0.0	5.435000601660583	0.0
54	0.0	0.0	0.0	5.689703581886006	0.0
55	0.0	0.0	0.0	5.994544944045566	0.0
56	0.0	0.0	0.0	6.33348040592034	0.0
57	0.0	0.0	0.0	6.4718623400585615	0.0
58	0.0	0.0	0.0	6.684449079459307	0.0
59	0.0	0.0	0.0	6.864947254422205	0.0
60	0.0	0.0	0.0	7.101600417151338	0.0
61	0.0	0.0	0.0	7.292126268501063	0.0
62	0.0	0.0	0.0	7.49067426096025	0.0
63	0.0	0.0	0.0	7.7152942120251895	0.0
64	0.0	0.0	0.0	7.9078255986522805	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAACTC	15	0.0022153002	69.97694	29
AACTCGA	15	0.0022153002	69.97694	31
AACCACC	15	0.0022153002	69.97694	70
ATAGCTT	15	0.0022153002	69.97694	3
TAACTCG	15	0.0022153002	69.97694	30
TACAAGT	15	0.0022153002	69.97694	7
ACATAAC	15	0.0022153002	69.97694	27
TTTACAC	15	0.0022153002	69.97694	2
CATACAA	20	7.8674006E-5	69.97694	5
GAGACTT	15	0.0022153002	69.97694	24
TTGACAA	40	1.02591E-8	61.35289	58
CTTGACA	40	1.02591E-8	61.35289	57
TGAACAA	40	1.0340955E-8	61.29129	59
GAACAAA	35	2.924844E-7	59.980225	60
AACAAAG	30	8.279743E-6	58.31411	69
CTATACA	30	8.279743E-6	58.31411	1
CCTTATA	30	8.279743E-6	58.31411	1
ACAAAGA	25	2.3727011E-4	55.981544	70
ACCCGAT	25	2.3727011E-4	55.981544	32
GGCCCTG	25	2.3727011E-4	55.981544	7
>>END_MODULE