FastQCFastQC Report
Wed 25 May 2016
SRR1779821_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779821_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308825
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCTCG150.002219462870.0309558
TTGTACG308.367819E-658.3307659
TAATCCG200.00694845252.4976925
CGACGAG608.367351E-1152.4806922
CGCGTTA200.006957360552.4806910
CCGTGTA305.8598974E-446.687334
TTCGGTA305.8833475E-446.649526
TTCCGAC451.6860267E-646.64949819
TCGGTGG1250.044.79802745
CGGTGGT1250.044.79077546
TGTCGTC404.5969347E-543.7339118
GTAGATC2250.043.560738
CTCGGTG1300.043.0820144
ACGTGTA503.4620443E-642.01857434
TAGATCT2550.041.18132839
TCTCGGT1450.041.03932643
CGGACGT602.5957706E-740.8249246
GATCTCG2450.040.00472341
TCGGTAA350.00125661439.98528727
GCGTTAT350.001257615239.97881311
Produced by FastQC (version 0.11.5)