##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779821_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	308825
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.844096170970616	34.0	23.0	34.0	12.0	34.0
2	29.7496446207399	34.0	31.0	34.0	12.0	34.0
3	29.201486278636768	34.0	27.0	34.0	11.0	34.0
4	29.470768234436978	34.0	27.0	34.0	12.0	34.0
5	30.2567214441836	34.0	32.0	34.0	11.0	34.0
6	32.51920019428479	37.0	31.0	38.0	12.0	38.0
7	32.84833481745325	37.0	32.0	38.0	12.0	38.0
8	32.03653201651421	37.0	31.0	38.0	11.0	38.0
9	33.06594025742734	37.0	34.0	38.0	12.0	38.0
10-11	31.498767910629	37.0	28.5	38.0	11.5	38.0
12-13	32.38293855743544	37.0	31.0	38.0	11.0	38.0
14-15	31.12284141504088	36.5	26.5	38.0	11.0	38.0
16-17	31.793975552497372	37.0	28.5	38.0	11.0	38.0
18-19	32.77952885938639	37.0	32.0	38.0	11.5	38.0
20-21	31.318991338136485	36.5	27.5	38.0	11.0	38.0
22-23	32.299354003076175	37.0	30.0	38.0	11.0	38.0
24-25	32.63306889014814	37.0	32.0	38.0	11.0	38.0
26-27	31.455265927305106	37.0	26.5	38.0	11.0	38.0
28-29	32.77216060875901	37.0	32.5	38.0	11.0	38.0
30-31	33.26941471707278	38.0	34.0	38.0	11.0	38.0
32-33	33.36985995304784	38.0	34.0	38.0	16.0	38.0
34-35	33.27074718691816	38.0	34.0	38.0	16.0	38.0
36-37	32.69151461183518	37.0	32.5	38.0	11.0	38.0
38-39	32.63841334088885	37.0	32.5	38.0	11.0	38.0
40-41	32.61690601473326	37.0	32.0	38.0	11.0	38.0
42-43	33.056748967862056	37.5	33.0	38.0	16.0	38.0
44-45	33.15648830243666	38.0	34.0	38.0	11.0	38.0
46-47	33.051627944628834	38.0	34.0	38.0	11.0	38.0
48-49	32.69638468388246	37.0	32.5	38.0	11.0	38.0
50-51	31.995654496883347	37.0	29.5	38.0	11.0	38.0
52-53	32.62007285679592	37.0	32.0	38.0	11.0	38.0
54-55	32.78811138994576	37.0	32.5	38.0	11.0	38.0
56-57	32.89998866672063	37.0	33.0	38.0	11.0	38.0
58-59	33.211993847648344	38.0	34.0	38.0	11.0	38.0
60-61	33.02512264227313	37.0	34.0	38.0	11.0	38.0
62-63	33.06267951104995	37.0	34.0	38.0	11.0	38.0
64-65	33.061024852262605	37.5	33.5	38.0	11.0	38.0
66-67	32.87177042014086	37.0	33.0	38.0	11.0	38.0
68-69	32.14241884562455	37.0	31.0	38.0	11.0	38.0
70-71	32.13147737391727	37.0	30.5	38.0	11.0	38.0
72-73	32.35733829838905	37.0	31.0	38.0	11.0	38.0
74-75	32.45054318788958	37.0	31.5	38.0	11.0	38.0
76	31.26311017566583	36.0	27.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	64.0
13	643.0
14	2167.0
15	3598.0
16	4882.0
17	5882.0
18	6427.0
19	6070.0
20	5088.0
21	4230.0
22	3734.0
23	3628.0
24	3638.0
25	3989.0
26	4521.0
27	5069.0
28	6108.0
29	7067.0
30	8593.0
31	10464.0
32	12869.0
33	16289.0
34	21473.0
35	29909.0
36	49230.0
37	83192.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.76362017323727	18.871529183194365	11.207641868372056	30.15720877519631
2	20.059580668663482	22.213227556059255	31.710191856229258	26.016999919048008
3	18.18861814943738	22.95410021857039	30.528292722415607	28.32898890957662
4	13.176394398121912	21.14466121589897	31.665506354731644	34.013438031247475
5	14.098923338460292	33.56528778434388	34.563263984457215	17.772524892738605
6	28.07983861305667	37.61798829750374	18.09968817089401	16.202484918545572
7	27.28994902948777	29.780833273964884	19.600655427679513	23.328562268867834
8	23.960067094534647	36.19478139227636	18.78517443931377	21.059977073875228
9	23.518229453407862	17.542893784044615	19.444210553587276	39.49466620896025
10-11	23.782053565357053	26.17002192222629	24.319423351542486	25.728501160874167
12-13	23.78308715791655	22.96190878638191	25.568891069199495	27.686112986502042
14-15	24.532945193613827	24.926859516843763	22.181602112533536	28.358593177008874
16-17	24.992026215536654	25.969604095529984	23.295593451944388	25.742776236988966
18-19	22.435276783834983	25.8632190793841	25.350776354775512	26.350727782005407
20-21	24.419655144499313	25.232089371002996	24.866186351493564	25.482069133004124
22-23	25.226345443947928	24.814778835567644	24.088303866329902	25.870571854154523
24-25	22.525318761384337	27.443837279902855	23.876138433515482	26.15470552519733
26-27	23.83675465111949	27.449012138001155	24.35225946211995	24.361973748759404
28-29	22.625871091422503	27.004805574985102	25.603773218310405	24.765550115281986
30-31	22.858114288952393	26.75598442470999	25.10276939018368	25.283131896153936
32-33	22.209989476240587	27.11292803367603	25.10661377802963	25.570468712053753
34-35	22.4864765093608	26.938016187711394	25.46609696112067	25.109410341807138
36-37	23.936780911203414	25.85003279166363	25.05849870857522	25.154687588557735
38-39	23.689911786422105	25.39281596911877	26.196582857290895	24.72068938716823
40-41	22.479992617142152	26.3710501305762	26.103907247991167	25.045050004290477
42-43	21.270760273684278	26.07593993970656	26.50239781363435	26.15090197297481
44-45	20.790102588015856	24.416789720473563	27.837685552187768	26.955422139322817
46-47	21.48537807180304	24.68047419669254	27.347263913579887	26.486883817924532
48-49	21.35303165223023	25.340079332955558	27.71699182384846	25.589897190965754
50-51	22.887567373279946	25.14017042262415	26.490955101750046	25.481307102345863
52-53	19.34355619605875	26.226986414495524	27.41972569911103	27.0097316903347
54-55	19.561788983843964	25.689359814249308	27.16296255517307	27.585888646733657
56-57	19.78959538321038	26.325351537958326	26.55706541099661	27.32798766783468
58-59	19.725375261104006	26.45772948815519	26.864970772544005	26.951924478196805
60-61	19.29733362482895	25.54197941717071	26.9994574942713	28.161229463729036
62-63	19.772440604471846	25.860553079111458	26.336105907250197	28.03090040916649
64-65	20.670580138455932	25.247075017205784	25.90097566900125	28.18136917533703
66-67	19.988146344070177	27.13240306964347	24.752765448212315	28.12668513807404
68-69	20.297969069761418	27.74735771831205	23.997163432893817	27.95750977903272
70-71	20.35988252170337	27.139817953973633	24.313927395304106	28.18637212901889
72-73	20.1716265664972	26.821184547132543	24.548265924030957	28.4589229623393
74-75	19.868877198266162	26.45076256215703	24.59613108394094	29.084229155635867
76	19.590566581502962	25.82856513728195	24.44586923647338	30.134999044741704
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	2.0
12	3.0
13	2.5
14	6.0
15	12.5
16	31.0
17	47.0
18	59.0
19	103.0
20	174.0
21	213.0
22	267.0
23	404.0
24	577.5
25	668.0
26	874.0
27	1186.5
28	1526.0
29	1759.0
30	2019.5
31	2652.0
32	3578.0
33	4132.0
34	4830.0
35	6091.0
36	7446.5
37	8239.0
38	9156.5
39	11238.5
40	13288.5
41	14881.0
42	15588.0
43	16199.5
44	17727.0
45	18816.0
46	18989.0
47	18969.0
48	18494.0
49	17563.5
50	17088.0
51	16316.0
52	14559.0
53	12603.5
54	11633.0
55	10961.5
56	9482.0
57	7879.5
58	7085.0
59	6587.0
60	5628.0
61	4795.5
62	4424.0
63	4165.5
64	3649.0
65	3151.0
66	2801.0
67	2691.0
68	2544.0
69	2208.0
70	1936.0
71	1853.0
72	1779.5
73	1619.0
74	1418.0
75	1304.0
76	1257.0
77	1112.0
78	935.0
79	856.0
80	763.5
81	624.0
82	511.0
83	445.0
84	390.0
85	283.0
86	190.5
87	150.0
88	136.5
89	110.0
90	81.0
91	54.0
92	43.0
93	40.0
94	26.5
95	14.0
96	12.0
97	11.5
98	7.0
99	10.5
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	6.47615963733506E-4
7	0.006152351655468308
8	0.002266655873067271
9	0.012628511292803367
10-11	0.001942847891200518
12-13	1.619039909333765E-4
14-15	4.857119728001295E-4
16-17	1.619039909333765E-4
18-19	0.00323807981866753
20-21	0.0
22-23	0.001619039909333765
24-25	0.004047599773334413
26-27	4.857119728001295E-4
28-29	0.005504735691734802
30-31	8.095199546668825E-4
32-33	0.0
34-35	0.003399983809600907
36-37	0.0186189589573383
38-39	0.009390431474135838
40-41	4.857119728001295E-4
42-43	6.47615963733506E-4
44-45	0.005504735691734802
46-47	0.002590463854934024
48-49	0.0
50-51	0.0014571359184003885
52-53	0.01295231927467012
54-55	0.007771391564802072
56-57	0.012628511292803367
58-59	0.01295231927467012
60-61	0.02347607868533959
62-63	0.008580911519468956
64-65	0.020237998866672064
66-67	0.019266574921071804
68-69	3.23807981866753E-4
70-71	6.47615963733506E-4
72-73	0.004857119728001295
74-75	0.009876143446935968
76	0.002590463854934024
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	308825.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.04743399440004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.67983853989739	49.43929522488735
2	8.111868156983872	10.066412080834647
3	4.81618881256231	8.964964723560836
4	2.8277003670307588	7.018062075171271
5	1.553078490963026	4.818226757807539
6	0.85022489500515	3.1652563831936638
7	0.5089541233381589	2.2105508161801506
8	0.30611098526369757	1.519472092248803
9	0.22969098553317085	1.2826562637580299
>10	1.1087692634891737	11.218311388378146
>50	0.007575379933285875	0.2967921939796165
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009714239456002591	0.0
2	0.0	0.0	0.0	0.010038047437869343	0.0
3	0.0	0.0	0.0	0.010361855419736096	0.0
4	0.0	0.0	0.0	0.01748563102080466	0.0
5	0.0	0.0	0.0	0.020723710839472193	0.0
6	0.0	0.0	0.0	0.0213713268032057	0.0
7	0.0	0.0	0.0	0.0213713268032057	0.0
8	0.0	0.0	0.0	0.02234275074880596	0.0
9	0.0	0.0	0.0	0.024285598640006477	0.0
10	0.0	0.0	0.0	0.02460940662187323	0.0
11	0.0	0.0	0.0	0.02460940662187323	0.0
12	0.0	0.0	0.0	0.02460940662187323	0.0
13	0.0	0.0	0.0	0.025257022585606734	0.0
14	0.0	0.0	0.0	0.026552254513073748	0.0
15	0.0	0.0	0.0	0.027523678458674008	0.0
16	0.0	0.0	0.0	0.02817129442240751	0.0
17	0.0	0.0	0.0	0.028495102404274265	0.0
18	0.0	0.0	0.0	0.02914271836800777	0.0
19	0.0	0.0	0.0	0.03108556625920829	0.0
20	0.0	0.0	0.0	0.0323807981866753	0.0
21	0.0	0.0	0.0	0.03497126204160932	0.0
22	0.0	0.0	0.0	0.04241884562454464	0.0
23	0.0	0.0	0.0	0.043390269570144906	0.0
24	0.0	0.0	0.0	0.04662834938881243	0.0
25	0.0	0.0	0.0	0.048571197280012954	0.0
26	0.0	0.0	0.0	0.050837853153080224	0.0
27	0.0	0.0	0.0	0.09293289079575812	0.0
28	0.0	0.0	0.0	0.13114223265603497	0.0
29	0.0	0.0	0.0	0.17809439002671415	0.0
30	0.0	0.0	0.0	0.2253703553792601	0.0
31	0.0	0.0	0.0	0.2778272484416741	0.0
32	0.0	0.0	0.0	0.3348174532502226	0.0
33	0.0	0.0	0.0	0.4138266008257104	0.0
34	0.0	0.0	0.0	0.4941309803286651	0.0
35	0.0	0.0	0.0	0.6194446693110985	0.0
36	0.0	0.0	0.0	0.8580911519468954	0.0
37	0.0	0.0	0.0	1.304298550959281	0.0
38	0.0	0.0	0.0	1.813324698453817	0.0
39	0.0	0.0	0.0	2.427912248036914	0.0
40	0.0	0.0	0.0	3.1684611025661784	0.0
41	0.0	0.0	0.0	3.9559621144661214	0.0
42	0.0	0.0	0.0	4.8143770743948835	0.0
43	0.0	0.0	0.0	5.746620254189266	0.0
44	0.0	0.0	0.0	6.634825548449769	0.0
45	0.0	0.0	0.0	7.5518497530964135	0.0
46	0.0	0.0	0.0	8.499311908038534	0.0
47	0.0	0.0	0.0	9.458431150327856	0.0
48	0.0	0.0	0.0	10.29774143932648	0.0
49	0.0	0.0	0.0	11.129927952724035	0.0
50	0.0	0.0	0.0	11.891200518092772	0.0
51	0.0	0.0	0.0	12.775520116570874	0.0
52	0.0	0.0	0.0	13.639763620173238	0.0
53	0.0	0.0	0.0	14.437950295474783	0.0
54	0.0	0.0	0.0	15.296041447421679	0.0
55	0.0	0.0	0.0	16.03594268598721	0.0
56	0.0	0.0	0.0	16.770015380879137	0.0
57	0.0	0.0	0.0	17.458431150327854	0.0
58	0.0	0.0	0.0	18.149761191613372	0.0
59	0.0	0.0	0.0	18.747834534121267	0.0
60	0.0	0.0	0.0	19.40354569740144	0.0
61	0.0	0.0	0.0	20.01003804743787	0.0
62	0.0	0.0	0.0	20.64502549987857	0.0
63	0.0	0.0	0.0	21.282279608192344	0.0
64	0.0	0.0	0.0	21.833400793329556	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCTCG	15	0.0022194628	70.03095	58
TTGTACG	30	8.367819E-6	58.330765	9
TAATCCG	20	0.006948452	52.497692	5
CGACGAG	60	8.367351E-11	52.48069	22
CGCGTTA	20	0.0069573605	52.48069	10
CCGTGTA	30	5.8598974E-4	46.6873	34
TTCGGTA	30	5.8833475E-4	46.6495	26
TTCCGAC	45	1.6860267E-6	46.649498	19
TCGGTGG	125	0.0	44.798027	45
CGGTGGT	125	0.0	44.790775	46
TGTCGTC	40	4.5969347E-5	43.73391	18
GTAGATC	225	0.0	43.5607	38
CTCGGTG	130	0.0	43.08201	44
ACGTGTA	50	3.4620443E-6	42.018574	34
TAGATCT	255	0.0	41.181328	39
TCTCGGT	145	0.0	41.039326	43
CGGACGT	60	2.5957706E-7	40.82492	46
GATCTCG	245	0.0	40.004723	41
TCGGTAA	35	0.001256614	39.985287	27
GCGTTAT	35	0.0012576152	39.978813	11
>>END_MODULE