FastQCFastQC Report
Wed 25 May 2016
SRR1779821_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779821_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308825
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT271588.793977171537279No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG32171.0416902776653445No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC18120.5867400631425564No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT6960.2253703553792601RNA PCR Primer, Index 15 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC6700.2169513478507245No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGT3360.10879948190722902No Hit
CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC3250.10523759410669474No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAAACTCGTATGCCGT3220.10426617016109448No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGTA150.002226033269.9787941
TGGACCG150.002227463569.967459
CATCTCG252.3937835E-455.97396536
TATGCCG43850.053.22217643
CCGTCTT43750.053.15554447
GCCGTCT43950.052.99334746
ATGCCGT43950.052.9332844
CTCGTAT43500.052.92648339
CGTCTTC43700.052.8957948
CGTATGC44200.052.87989841
TCGTATG44200.052.80073540
TGCCGTC44050.052.70548645
GTATGCC44400.052.5628942
CTTGAAA42750.052.5316957
TCTCGTA41400.052.4840938
TTCGGAG200.0069555852.48408539
GCAACGT200.006960036252.47558610
GTCCGTA200.006960036252.47558611
ATCTCGT41550.052.29461737
AATCTCG40750.052.28243336