##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779821_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	308825
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.488807577106776	34.0	34.0	34.0	34.0	34.0
2	33.592348417388486	34.0	34.0	34.0	34.0	34.0
3	33.71858819719906	34.0	34.0	34.0	34.0	34.0
4	33.48417712296608	34.0	34.0	34.0	34.0	34.0
5	33.54938233627459	34.0	34.0	34.0	34.0	34.0
6	37.17603173318222	38.0	38.0	38.0	36.0	38.0
7	37.26716101351899	38.0	38.0	38.0	36.0	38.0
8	37.142410750425	38.0	38.0	38.0	36.0	38.0
9	37.056034971262044	38.0	38.0	38.0	36.0	38.0
10-11	36.98654254027362	38.0	37.5	38.0	35.0	38.0
12-13	37.1827394155266	38.0	38.0	38.0	36.0	38.0
14-15	37.206332065085405	38.0	38.0	38.0	36.0	38.0
16-17	37.12723872743463	38.0	38.0	38.0	36.0	38.0
18-19	36.98141989800049	38.0	37.5	38.0	35.5	38.0
20-21	36.99207641868372	38.0	37.5	38.0	35.5	38.0
22-23	36.96458350198333	38.0	37.5	38.0	35.5	38.0
24-25	37.19430421759896	38.0	38.0	38.0	36.0	38.0
26-27	37.086566825872254	38.0	38.0	38.0	36.0	38.0
28-29	36.53034242694082	38.0	37.0	38.0	34.0	38.0
30-31	36.8252910224237	38.0	37.0	38.0	35.0	38.0
32-33	36.89219946571683	38.0	37.0	38.0	35.5	38.0
34-35	37.01955800210475	38.0	38.0	38.0	36.0	38.0
36-37	36.965563021128474	38.0	37.5	38.0	35.5	38.0
38-39	36.50884481502469	38.0	37.0	38.0	34.0	38.0
40-41	36.76347122156561	38.0	37.0	38.0	34.5	38.0
42-43	37.083315793734315	38.0	38.0	38.0	36.0	38.0
44-45	36.821112280417715	38.0	37.0	38.0	35.0	38.0
46-47	36.981113899457625	38.0	38.0	38.0	36.0	38.0
48-49	36.58653606411398	38.0	37.0	38.0	34.0	38.0
50-51	36.794514692787175	38.0	37.0	38.0	34.5	38.0
52-53	36.704543025985586	38.0	37.0	38.0	34.5	38.0
54-55	36.71854610216141	38.0	37.0	38.0	34.5	38.0
56-57	36.57958228770339	38.0	37.0	38.0	34.0	38.0
58-59	36.691048328341296	38.0	37.0	38.0	34.5	38.0
60-61	36.341185137213635	38.0	37.0	38.0	34.0	38.0
62-63	36.13736744110743	38.0	37.0	38.0	34.0	38.0
64-65	36.52563425888448	38.0	37.0	38.0	34.0	38.0
66-67	36.28906986157209	38.0	37.0	38.0	33.0	38.0
68-69	36.41360317331822	38.0	37.0	38.0	34.0	38.0
70-71	35.71589573382984	38.0	37.0	38.0	30.5	38.0
72-73	34.525618068485386	38.0	36.5	38.0	23.0	38.0
74-75	34.34122399417146	38.0	36.5	38.0	22.5	38.0
76	34.07560268760625	38.0	35.0	38.0	22.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	0.0
21	2.0
22	15.0
23	42.0
24	81.0
25	153.0
26	323.0
27	581.0
28	1076.0
29	1736.0
30	2914.0
31	4600.0
32	7277.0
33	11454.0
34	19005.0
35	36128.0
36	52658.0
37	170778.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.67769441096813	16.946895774209853	11.236377301766254	34.13903251305576
2	19.2549178337246	17.282927224156076	38.82781510564236	24.63433983647697
3	17.802639035052216	19.447907390917184	35.96761920181332	26.781834372217272
4	13.025823686553872	23.926819396098114	33.90366712539464	29.14368979195337
5	13.043633125556545	31.663887314822308	41.09641382660082	14.196065733020319
6	27.674572978223917	42.5117785153404	16.338703149032625	13.47494535740306
7	26.65360641139804	27.040880757710678	19.39966000161904	26.90585282927224
8	23.552821177042013	40.282684368169676	16.806281874848214	19.358212579940094
9	22.117704201408564	16.111389945762163	17.942200275236786	43.82870557759249
10-11	23.164575406783776	27.516716587063872	27.756982109609	21.56172589654335
12-13	22.941957419250382	20.43892171942038	28.26325588925767	28.355864972071558
14-15	20.916052780701044	20.873957743058366	21.049299765239212	37.160689711001375
16-17	26.852748320246096	28.40233141746944	21.70080142475512	23.044118837529346
18-19	26.6388731482231	24.30777948676435	22.75706306160447	26.29628430340808
20-21	20.89613858981624	23.124747025014166	22.55565449688335	33.423459888286246
22-23	21.303489031004613	28.646644539787907	22.44458835910305	27.60527807010443
24-25	26.524017071322636	28.540227056362568	22.53871162028612	22.397044252028678
26-27	26.348579292479556	29.353355460212093	22.911681372945843	21.3863838743625
28-29	20.85161499230956	29.280822472273943	23.544725977495347	26.322836557921153
30-31	20.73374888691006	29.017728487007204	28.973204889500526	21.275317736582206
32-33	25.43981219137052	30.14668501578564	23.4974500121428	20.916052780701044
34-35	21.955638306484254	23.35416498016676	33.920019428478916	20.770177284870073
36-37	20.679834857929247	33.15259451145471	24.956852586416257	21.21071804419979
38-39	20.401198089532908	24.532340322188944	28.709139480288187	26.357322107989962
40-41	20.327855581640087	21.821257994009553	30.22585606735206	27.6250303569983
42-43	25.765891789147826	21.817027876809693	29.668774670280385	22.74830566376209
44-45	20.093094794786694	27.14352788796244	30.88950052618797	21.873876791062898
46-47	25.04249979762001	21.71990609568526	26.146037399821903	27.091556706872826
48-49	24.174046790253378	21.545211689468147	25.78094390026714	28.499797620011336
50-51	19.74661120008291	21.91162158792258	30.995111319101802	27.346655892892706
52-53	17.979227703305657	22.15295963929472	36.61501934547669	23.252793311922936
54-55	17.55134240255554	22.14291962431519	32.07527303240103	28.23046494072824
56-57	23.032538459918587	22.12832337919204	26.324451550302836	28.514686610586544
58-59	18.303264124753607	22.361837938870345	35.334923851126234	23.99997408524981
60-61	23.43358010375047	27.315233956818684	25.668118352625264	23.583067586805583
62-63	17.81459527742688	34.32044180999579	23.55294270074175	24.312020211835584
64-65	17.896642517318547	35.161753132560165	22.717983761533553	24.223620588587732
66-67	17.835988785391855	35.18818157817589	22.01166487692923	24.964164759503017
68-69	18.021855959222073	35.549532132210906	21.705859398402495	24.72275251016453
70-71	18.298330553621454	34.94956037372282	21.51853231212656	25.233576760529168
72-73	18.586570149229562	32.63908794260775	21.918203091472943	26.856138816689747
74-75	18.874655292744105	32.25601689247725	22.035355669877728	26.833972144900926
76	18.91408752198089	32.4747159081709	22.32318947119573	26.28800709865249
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.5
7	2.5
8	2.0
9	1.5
10	0.5
11	0.0
12	0.0
13	5.0
14	14.5
15	14.0
16	23.0
17	37.0
18	41.0
19	68.0
20	167.0
21	243.0
22	266.5
23	384.5
24	585.5
25	692.0
26	889.0
27	1214.0
28	1566.0
29	1790.0
30	2150.5
31	2719.0
32	3417.5
33	3908.0
34	4718.0
35	5961.5
36	11343.0
37	16291.0
38	18954.0
39	21302.0
40	19736.5
41	17573.5
42	16661.0
43	16157.0
44	16071.0
45	16502.5
46	16516.0
47	16599.0
48	15954.0
49	14820.5
50	14415.0
51	13767.5
52	11923.5
53	10222.5
54	9718.0
55	8888.5
56	7432.5
57	6124.5
58	5443.0
59	5015.5
60	4298.0
61	3788.0
62	3568.0
63	3308.0
64	2952.0
65	2730.5
66	2628.5
67	2652.0
68	2393.5
69	2060.5
70	1896.5
71	1807.0
72	1652.5
73	1433.5
74	1283.0
75	1197.0
76	1155.0
77	1038.0
78	895.0
79	827.0
80	775.5
81	596.5
82	411.0
83	353.0
84	303.5
85	204.5
86	165.5
87	176.0
88	118.5
89	57.5
90	40.5
91	19.5
92	12.0
93	9.5
94	8.0
95	4.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0482473892981462
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	9.71423945600259E-4
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	6.47615963733506E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.016352303084271026
52-53	0.031247470250141667
54-55	0.052456893062413995
56-57	0.051647373107747106
58-59	0.03901886181494374
60-61	0.033514126123208934
62-63	0.030761758277341535
64-65	0.016838015057071155
66-67	0.03901886181494374
68-69	0.009876143446935968
70-71	0.01295231927467012
72-73	0.02347607868533959
74-75	0.015704687120537523
76	0.011009471383469603
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	308825.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.627297012871367
#Duplication Level	Percentage of deduplicated	Percentage of total
1	37.77872188797676	8.926090828138914
2	13.962476187865747	6.5979114385169595
3	12.779749749886934	9.058528292722416
4	10.514342099853359	9.937019347526917
5	7.61577151314978	8.997004776167733
6	5.237984294270012	7.42556464016838
7	3.23845025833596	5.3561078280579615
8	1.995422588293338	3.7717153727839388
9	1.2909945591842888	2.7452440702663323
>10	5.385996409335727	22.65975876305351
>50	0.16719887072238135	2.6451874038695053
>100	0.026039168391190536	1.0151380231522706
>500	0.00274096509380953	0.4423217032299846
>1k	0.00274096509380953	1.6284303408079008
>5k	0.0	0.0
>10k+	0.001370482546904765	8.793977171537279
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT	27158	8.793977171537279	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG	3217	1.0416902776653445	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC	1812	0.5867400631425564	No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT	696	0.2253703553792601	RNA PCR Primer, Index 15 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC	670	0.2169513478507245	No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGT	336	0.10879948190722902	No Hit
CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC	325	0.10523759410669474	No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAAACTCGTATGCCGT	322	0.10426617016109448	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.017161823038937908	0.0
2	0.0	0.0	0.0	0.017809439002671415	0.0
3	0.0	0.0	0.0	0.01813324698453817	0.0
4	0.0	0.0	0.0	0.027847486440540758	0.0
5	0.0	0.0	0.0	0.032056990204808546	0.0
6	0.0	0.0	0.0	0.03270460616854205	0.0
7	0.0	0.0	0.0	0.03270460616854205	0.0
8	0.0	0.0	0.0	0.033676030114142316	0.0
9	0.0	0.0	0.0	0.036914109932809844	0.0
10	0.0	0.0	0.0	0.03756172589654335	0.0
11	0.0	0.0	9.71423945600259E-4	0.03756172589654335	0.0
12	0.0	0.0	9.71423945600259E-4	0.03756172589654335	0.0
13	0.0	0.0	9.71423945600259E-4	0.03820934186027686	0.0
14	0.0	0.0	9.71423945600259E-4	0.03820934186027686	0.0
15	0.0	0.0	9.71423945600259E-4	0.03982838176961062	0.0
16	0.0	0.0	9.71423945600259E-4	0.04079980571521088	0.0
17	0.0	0.0	9.71423945600259E-4	0.041447421678944385	0.0
18	0.0	0.0	9.71423945600259E-4	0.04241884562454464	0.0
19	0.0	0.0	9.71423945600259E-4	0.04436169351574516	0.0
20	0.0	0.0	9.71423945600259E-4	0.045980733425078926	0.0
21	0.0	0.0	9.71423945600259E-4	0.05019023718934672	0.0
22	0.0	0.0	9.71423945600259E-4	0.051161661134946974	0.0
23	0.0	0.0	9.71423945600259E-4	0.053752124989881	0.0
24	0.0	0.0	9.71423945600259E-4	0.05666639682668178	0.0
25	0.0	0.0	9.71423945600259E-4	0.060552092609082814	0.0
26	0.0	0.0	9.71423945600259E-4	0.062494940500283334	0.0
27	0.0	0.0	9.71423945600259E-4	0.10944709787096252	0.0
28	0.0	0.0	9.71423945600259E-4	0.1515421355136404	0.0
29	0.0	0.0	9.71423945600259E-4	0.20302760463045413	0.0
30	0.0	0.0	9.71423945600259E-4	0.2535416498016676	0.0
31	0.0	0.0	9.71423945600259E-4	0.3127985104832834	0.0
32	0.0	0.0	9.71423945600259E-4	0.37173156318303247	0.0
33	0.0	0.0	9.71423945600259E-4	0.4585121023233223	0.0
34	0.0	0.0	9.71423945600259E-4	0.5524164170646807	0.0
35	0.0	0.0	9.71423945600259E-4	0.6997490488140533	0.0
36	0.0	0.0	9.71423945600259E-4	0.967538249817858	0.0
37	0.0	0.0	9.71423945600259E-4	1.4613454221646565	0.0
38	0.0	0.0	9.71423945600259E-4	2.0092285274832022	0.0
39	0.0	0.0	9.71423945600259E-4	2.6571682991985752	0.0
40	0.0	0.0	9.71423945600259E-4	3.4751072613939935	0.0
41	0.0	0.0	9.71423945600259E-4	4.32413178984862	0.0
42	0.0	0.0	9.71423945600259E-4	5.265117785153404	0.0
43	0.0	0.0	9.71423945600259E-4	6.269570144904072	0.0
44	0.0	0.0	9.71423945600259E-4	7.220917995628592	0.0
45	0.0	0.0	9.71423945600259E-4	8.206913300412856	0.0
46	0.0	0.0	9.71423945600259E-4	9.249898810005666	0.0
47	0.0	0.0	9.71423945600259E-4	10.281227232251275	0.0
48	0.0	0.0	9.71423945600259E-4	11.196632396988585	0.0
49	0.0	0.0	9.71423945600259E-4	12.106856634016028	0.0
50	0.0	0.0	9.71423945600259E-4	12.924795596211446	0.0
51	0.0	0.0	9.71423945600259E-4	13.866105399498098	0.0
52	0.0	0.0	9.71423945600259E-4	14.762729701287137	0.0
53	0.0	0.0	9.71423945600259E-4	15.581640087428156	0.0
54	0.0	0.0	9.71423945600259E-4	16.466607301869992	0.0
55	0.0	0.0	9.71423945600259E-4	17.222051323565125	0.0
56	0.0	0.0	9.71423945600259E-4	17.979762001133327	0.0
57	0.0	0.0	9.71423945600259E-4	18.703149032623653	0.0
58	0.0	0.0	9.71423945600259E-4	19.40581235327451	0.0
59	0.0	0.0	9.71423945600259E-4	20.029466526349875	0.0
60	0.0	0.0	9.71423945600259E-4	20.706549016433254	0.0
61	0.0	0.0	9.71423945600259E-4	21.341536468873958	0.0
62	0.0	0.0	9.71423945600259E-4	22.008904719501334	0.0
63	0.0	0.0	9.71423945600259E-4	22.679511049947383	0.0
64	0.0	0.0	9.71423945600259E-4	23.271755848781673	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGTA	15	0.0022260332	69.97879	41
TGGACCG	15	0.0022274635	69.96745	9
CATCTCG	25	2.3937835E-4	55.973965	36
TATGCCG	4385	0.0	53.222176	43
CCGTCTT	4375	0.0	53.155544	47
GCCGTCT	4395	0.0	52.993347	46
ATGCCGT	4395	0.0	52.93328	44
CTCGTAT	4350	0.0	52.926483	39
CGTCTTC	4370	0.0	52.89579	48
CGTATGC	4420	0.0	52.879898	41
TCGTATG	4420	0.0	52.800735	40
TGCCGTC	4405	0.0	52.705486	45
GTATGCC	4440	0.0	52.56289	42
CTTGAAA	4275	0.0	52.53169	57
TCTCGTA	4140	0.0	52.48409	38
TTCGGAG	20	0.00695558	52.484085	39
GCAACGT	20	0.0069600362	52.475586	10
GTCCGTA	20	0.0069600362	52.475586	11
ATCTCGT	4155	0.0	52.294617	37
AATCTCG	4075	0.0	52.282433	36
>>END_MODULE