FastQCFastQC Report
Wed 25 May 2016
SRR1779820_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779820_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199726
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATGCG207.9152735E-569.995255
TGACCGT252.387041E-455.99619739
TCTCGGT1250.055.99619743
CTCGGTG1200.055.412944
CGGTGGT1100.054.08723446
GCCGTAT653.6379788E-1253.8559854
AGCGTAG200.006945536552.49643335
AGCGTAA200.006945536552.49643335
AAGCGTA200.006945536552.49643334
TAGCACG200.006945536552.4964335
GTTACGC200.006945536552.4964339
GACGCTT200.006945536552.49643314
CTTACGC608.367351E-1152.4964312
TTACGCT506.170376E-848.9966713
ATCTCGG1600.048.1217342
GTAGATC1900.047.8914838
TGGTCGC950.047.8914849
TCGGTGG1400.047.49677345
GGCGGTC305.869643E-446.66349447
CATACGA305.869643E-446.6634949