##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779820_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	199726
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.019521744790364	33.0	23.0	34.0	12.0	34.0
2	28.89344401830508	34.0	23.0	34.0	12.0	34.0
3	28.466238747083505	34.0	23.0	34.0	11.0	34.0
4	28.735442556302132	34.0	23.0	34.0	12.0	34.0
5	29.22646525740264	34.0	27.0	34.0	11.0	34.0
6	31.58844116439522	37.0	27.0	38.0	12.0	38.0
7	31.883991067762835	37.0	28.0	38.0	12.0	38.0
8	31.120920661305988	37.0	27.0	38.0	11.0	38.0
9	32.070937183942	37.0	31.0	38.0	12.0	38.0
10-11	30.621676697074996	36.5	25.5	38.0	11.0	38.0
12-13	31.438315492224348	37.0	27.0	38.0	11.0	38.0
14-15	30.228923625366754	36.0	24.5	38.0	11.0	38.0
16-17	30.85482360834343	36.5	25.5	38.0	11.0	38.0
18-19	31.737442796631385	37.0	28.0	38.0	11.0	38.0
20-21	30.45470794989135	36.0	24.5	38.0	11.0	38.0
22-23	31.347506083334167	37.0	28.5	38.0	11.0	38.0
24-25	31.670333356698677	37.0	29.0	38.0	11.0	38.0
26-27	30.562463074411944	37.0	24.5	38.0	11.0	38.0
28-29	31.720617245626507	37.0	29.0	38.0	11.0	38.0
30-31	32.19065619899262	37.5	31.0	38.0	11.0	38.0
32-33	32.30209386859998	38.0	31.0	38.0	11.0	38.0
34-35	32.20804251825	37.0	31.0	38.0	11.0	38.0
36-37	31.664405235172186	37.0	29.0	38.0	11.0	38.0
38-39	31.60618046724012	37.0	28.5	38.0	11.0	38.0
40-41	31.55669767581587	37.0	27.0	38.0	11.0	38.0
42-43	32.009445440253145	37.0	29.0	38.0	11.0	38.0
44-45	32.08540450417071	37.0	31.0	38.0	11.0	38.0
46-47	31.967117450907743	37.0	29.0	38.0	11.0	38.0
48-49	31.610040755835495	37.0	28.5	38.0	11.0	38.0
50-51	30.941357159308254	37.0	25.5	38.0	11.0	38.0
52-53	31.540432893063496	37.0	27.0	38.0	11.0	38.0
54-55	31.710891421247105	37.0	29.0	38.0	11.0	38.0
56-57	31.792913791894897	37.0	29.0	38.0	11.0	38.0
58-59	32.08833852377757	37.0	31.0	38.0	11.0	38.0
60-61	31.93695863332766	37.0	29.5	38.0	11.0	38.0
62-63	31.940911548821887	37.0	31.0	38.0	11.0	38.0
64-65	31.949280514304597	37.0	29.0	38.0	11.0	38.0
66-67	31.816889138119222	37.0	27.5	38.0	11.0	38.0
68-69	31.139238256411282	37.0	26.0	38.0	11.0	38.0
70-71	31.154038532789922	37.0	26.0	38.0	11.0	38.0
72-73	31.33667123959825	37.0	27.0	38.0	11.0	38.0
74-75	31.398065349528856	37.0	27.0	38.0	11.0	38.0
76	30.245987002193004	36.0	25.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	41.0
13	491.0
14	1838.0
15	3387.0
16	4643.0
17	5715.0
18	6063.0
19	5573.0
20	4591.0
21	3684.0
22	3015.0
23	2588.0
24	2527.0
25	2592.0
26	2840.0
27	3294.0
28	3894.0
29	4530.0
30	5019.0
31	6248.0
32	7874.0
33	9885.0
34	12856.0
35	18135.0
36	29761.0
37	48640.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.112343911158284	18.944453901845527	9.90907543334368	34.03412675365251
2	19.514735187206472	20.845558415028588	33.53093738421637	26.10876901354856
3	17.027828124530604	20.848061844727276	34.44569059611668	27.678419434625432
4	12.425022280524319	24.962198211549826	30.806705186104967	31.806074321820894
5	15.678479516938204	33.418283047775454	33.55847511090194	17.34476232438441
6	26.05735000325454	37.88885495265896	16.439433009047622	19.61436203503888
7	25.518128877516787	31.136303321332537	18.90100095639155	24.444566844759123
8	22.469569745492418	39.62166844416405	16.794095704464773	21.11466610587876
9	22.695976565434016	18.5283292856964	18.65351393305125	40.12218021581833
10-11	22.41160035650267	27.769404860853804	23.90345386995664	25.91554091268689
12-13	22.29134487083522	23.164042648535112	26.016958275232753	28.527654205396907
14-15	24.741646055095483	25.512201716351402	21.961337031733475	27.784815196819647
16-17	25.252285022618658	26.143763033578754	22.464945462374423	26.13900648142817
18-19	21.62296809791684	26.0932152683375	25.308869144629618	26.97494748911604
20-21	24.3621261127745	24.915133733214503	24.464516387450807	26.258223766560185
22-23	25.046001787498028	25.169674620281846	23.401954231037873	26.38236936118225
24-25	21.496506332663223	28.5106287099092	23.08924811672429	26.903616840703286
26-27	23.447357353147343	28.443251687328512	23.71322424946426	24.396166710059884
28-29	21.89761833826957	27.66362814732104	25.438941283388456	24.999812231020936
30-31	22.632870196520216	26.887219927400174	25.412692452121664	25.067217423957942
32-33	21.04658381983317	27.95179395772208	25.220051470514605	25.781570751930143
34-35	21.433560568697597	28.589596909665804	25.219620517776182	24.757222003860413
36-37	23.743552506384898	25.812509389553806	25.328008413040216	25.115929691021083
38-39	23.88766346108105	25.34257036055953	26.426927920642353	24.342838257717066
40-41	21.76968331455752	27.191638502941544	25.636500187758166	25.402177994742768
42-43	20.242783219969407	26.452806476889528	26.79252273745716	26.5118875656839
44-45	19.765316749784695	24.47650663942799	28.472681207314388	27.285495403472932
46-47	20.686210694972964	24.758161425996395	27.908822351291807	26.64680552773883
48-49	20.72188898791344	25.59281215264913	27.575528474009392	26.10977038542804
50-51	23.212341061517545	25.707540674983164	25.770878239165647	25.30924002433364
52-53	18.247780431745458	27.37692850811964	26.810581819820634	27.564709240314272
54-55	19.031989124148446	25.731754031500913	26.642581562627534	28.59367528172311
56-57	19.368310361662974	26.424306155406168	26.02020505501934	28.18717842791152
58-59	18.97617962674572	26.650325733714563	26.38518199526296	27.988312644276753
60-61	18.349225745708047	25.820078527214086	26.826458863358642	29.004236863719225
62-63	19.18980496231942	26.125284795072734	26.089982724518666	28.59492751808918
64-65	19.935097941513032	25.548925685640672	25.26172406108606	29.254252311760244
66-67	19.22491411860146	28.05918054623574	23.49745110018328	29.218454234979518
68-69	19.366079944724135	29.08316664622194	23.038370163048786	28.51238324600514
70-71	19.259433572578363	28.371690144723633	23.487486575324635	28.881389707373366
72-73	19.471253145067408	28.06914767108541	23.643897004518884	28.8157021793283
74-75	18.834683597544487	27.361627126791312	24.04313167829997	29.76055759736423
76	18.273900722003585	26.44225473408037	23.19974764923243	32.08409689468361
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	2.5
12	4.0
13	4.5
14	4.0
15	4.5
16	12.0
17	18.0
18	34.0
19	60.0
20	98.0
21	126.0
22	124.0
23	192.0
24	329.0
25	396.0
26	533.5
27	712.5
28	896.0
29	1038.0
30	1225.0
31	1638.0
32	2278.5
33	2693.0
34	2999.0
35	3753.0
36	4734.0
37	5267.0
38	5975.0
39	7430.5
40	8656.0
41	9744.5
42	10355.0
43	10760.5
44	11563.5
45	12197.0
46	12433.0
47	12573.5
48	12420.0
49	11882.5
50	11639.0
51	11124.5
52	9838.0
53	8468.0
54	7870.0
55	7359.5
56	6305.5
57	5217.5
58	4673.0
59	4323.0
60	3624.5
61	2980.0
62	2684.0
63	2482.5
64	2133.5
65	1855.5
66	1617.0
67	1509.0
68	1374.5
69	1179.0
70	1055.0
71	992.0
72	929.0
73	856.5
74	763.0
75	679.0
76	633.5
77	546.0
78	470.5
79	437.0
80	387.0
81	324.0
82	262.5
83	214.0
84	195.5
85	153.5
86	118.0
87	106.0
88	85.5
89	62.0
90	47.5
91	29.5
92	23.0
93	20.0
94	15.5
95	15.0
96	16.0
97	12.0
98	11.5
99	10.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0025034296986872016
7	0.008511660975536486
8	0.003504801578162082
9	0.010514404734486248
10-11	0.004005487517899522
12-13	2.5034296986872014E-4
14-15	0.0
16-17	7.510289096061604E-4
18-19	0.0027537726685559214
20-21	0.0
22-23	0.0027537726685559214
24-25	0.0037551445480308024
26-27	0.0010013718794748805
28-29	0.006258574246718003
30-31	5.006859397374403E-4
32-33	0.0
34-35	0.0032544586082933623
36-37	0.01802469383054785
38-39	0.011265433644092406
40-41	5.006859397374403E-4
42-43	2.5034296986872014E-4
44-45	0.005006859397374403
46-47	0.0030041156384246416
48-49	0.0
50-51	0.0012517148493436008
52-53	0.012517148493436006
54-55	0.008762003945405205
56-57	0.011766119583829848
58-59	0.011515776613961127
60-61	0.025034296986872012
62-63	0.010514404734486248
64-65	0.020277780559366333
66-67	0.01602195007159809
68-69	2.5034296986872014E-4
70-71	2.5034296986872014E-4
72-73	0.004255830487768243
74-75	0.006008231276849283
76	0.002002743758949761
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	199726.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.47892208519913
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.09155441725811	48.16597221862759
2	9.887733228481082	12.355498279630082
3	5.468811655211442	10.250563719137537
4	3.1060144464899584	7.762417383909962
5	1.6030122336629364	5.007723822432381
6	0.8647160874337074	3.2415917431553307
7	0.5171252441899709	2.261659948802434
8	0.37071669778911365	1.8529583741478677
9	0.25108385313930154	1.4118703647427802
>10	0.8372551510152348	7.617484996693356
>50	0.0019769853291427796	0.07225914872070968
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.006859397374403E-4	0.0
2	0.0	0.0	0.0	5.006859397374403E-4	0.0
3	0.0	0.0	0.0	5.006859397374403E-4	0.0
4	0.0	0.0	0.0	0.0030041156384246416	0.0
5	0.0	0.0	0.0	0.007009603156324164	0.0
6	0.0	0.0	0.0	0.007009603156324164	0.0
7	0.0	0.0	0.0	0.007009603156324164	0.0
8	0.0	0.0	0.0	0.007009603156324164	0.0
9	0.0	0.0	0.0	0.008511660975536486	0.0
10	0.0	0.0	0.0	0.009513032855011365	0.0
11	0.0	0.0	0.0	0.009513032855011365	0.0
12	0.0	0.0	0.0	0.011015090674223687	0.0
13	0.0	0.0	0.0	0.011515776613961127	0.0
14	0.0	0.0	0.0	0.011515776613961127	0.0
15	0.0	0.0	0.0	0.012517148493436008	0.0
16	0.0	0.0	0.0	0.012517148493436008	0.0
17	0.0	0.0	0.0	0.014019206312648328	0.0
18	0.0	0.0	0.0	0.014019206312648328	0.0
19	0.0	0.0	0.0	0.014019206312648328	0.0
20	0.0	0.0	0.0	0.01652263601133553	0.0
21	0.0	0.0	0.0	0.01752400789081041	0.0
22	0.0	0.0	0.0	0.026536354806084336	0.0
23	0.0	0.0	0.0	0.031042528263721298	0.0
24	0.0	0.0	0.0	0.037050759540570584	0.0
25	0.0	0.0	0.0	0.039554189239257784	0.0
26	0.0	0.0	0.0	0.04355967675715731	0.0
27	0.0	0.0	0.0	0.07310014720166629	0.0
28	0.0	0.0	0.0	0.1081481629832871	0.0
29	0.0	0.0	0.0	0.1426954928251705	0.0
30	0.0	0.0	0.0	0.1727366492094169	0.0
31	0.0	0.0	0.0	0.22430730100237325	0.0
32	0.0	0.0	0.0	0.2773800106145419	0.0
33	0.0	0.0	0.0	0.3314540921061855	0.0
34	0.0	0.0	0.0	0.3970439502117902	0.0
35	0.0	0.0	0.0	0.5081962288335019	0.0
36	0.0	0.0	0.0	0.704965803150316	0.0
37	0.0	0.0	0.0	1.0334157796180767	0.0
38	0.0	0.0	0.0	1.4840331253817731	0.0
39	0.0	0.0	0.0	2.0432993200684937	0.0
40	0.0	0.0	0.0	2.749767181038022	0.0
41	0.0	0.0	0.0	3.6940608633828345	0.0
42	0.0	0.0	0.0	4.708951263230626	0.0
43	0.0	0.0	0.0	5.667264151888086	0.0
44	0.0	0.0	0.0	6.592031082583139	0.0
45	0.0	0.0	0.0	7.453210898931536	0.0
46	0.0	0.0	0.0	8.339425012266805	0.0
47	0.0	0.0	0.0	9.091955979692179	0.0
48	0.0	0.0	0.0	9.901064458307882	0.0
49	0.0	0.0	0.0	10.65459679761273	0.0
50	0.0	0.0	0.0	11.402120905640729	0.0
51	0.0	0.0	0.0	12.224747904629343	0.0
52	0.0	0.0	0.0	13.116469563301724	0.0
53	0.0	0.0	0.0	14.130859277209778	0.0
54	0.0	0.0	0.0	15.080159818951964	0.0
55	0.0	0.0	0.0	15.907292991398215	0.0
56	0.0	0.0	0.0	16.584220381923235	0.0
57	0.0	0.0	0.0	17.294693730410664	0.0
58	0.0	0.0	0.0	17.980633467850957	0.0
59	0.0	0.0	0.0	18.627019016051992	0.0
60	0.0	0.0	0.0	19.26889839079539	0.0
61	0.0	0.0	0.0	19.841683105855022	0.0
62	0.0	0.0	0.0	20.469543274285773	0.0
63	0.0	0.0	0.0	21.03481770024934	0.0
64	0.0	0.0	0.0	21.65116209206613	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAATGCG	20	7.9152735E-5	69.99525	5
TGACCGT	25	2.387041E-4	55.996197	39
TCTCGGT	125	0.0	55.996197	43
CTCGGTG	120	0.0	55.4129	44
CGGTGGT	110	0.0	54.087234	46
GCCGTAT	65	3.6379788E-12	53.85598	54
AGCGTAG	20	0.0069455365	52.496433	35
AGCGTAA	20	0.0069455365	52.496433	35
AAGCGTA	20	0.0069455365	52.496433	34
TAGCACG	20	0.0069455365	52.496433	5
GTTACGC	20	0.0069455365	52.496433	9
GACGCTT	20	0.0069455365	52.496433	14
CTTACGC	60	8.367351E-11	52.49643	12
TTACGCT	50	6.170376E-8	48.99667	13
ATCTCGG	160	0.0	48.12173	42
GTAGATC	190	0.0	47.89148	38
TGGTCGC	95	0.0	47.89148	49
TCGGTGG	140	0.0	47.496773	45
GGCGGTC	30	5.869643E-4	46.663494	47
CATACGA	30	5.869643E-4	46.663494	9
>>END_MODULE