FastQCFastQC Report
Wed 25 May 2016
SRR1779820_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779820_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199726
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT2588212.95875349228443No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG33741.6893143606741237No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC18390.9207614431771528No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC6490.32494517488959873No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT6390.31993831549222435Illumina PCR Primer Index 9 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT5060.2533470855071448No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCGT4650.23281896197790974No Hit
CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC2680.13418383184963398No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATATCGTATGCCGT2150.10764747704354967No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAGCTCGTATGCCGT2150.10764747704354967No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGTA150.002224508469.9817324
GCTTGAC352.9380863E-760.0745856
TACATCG252.3893207E-455.98537439
CATCGCG252.3893207E-455.98537441
ATCTCGT35350.053.1544337
TCTCGTA35500.053.12697238
GATCTCG35450.053.0044936
AGATCTC35650.052.80528335
CTCGTAT37650.052.78833839
GGTCGAG200.00693021552.52574545
CTCCGTA200.006950845552.48629422
CATGCTG200.006950845552.48629441
TGGACTA200.006950845552.4862945
CGGCCGT200.006950845552.48629413
CGGGCAA200.006950845552.48629433
AATGTAC200.006950845552.48629426
TCTATAG200.006950845552.48629432
TCACGTG200.006950845552.48629415
CGTATGC38300.052.34925541
TCGTATG38250.052.32620240