##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779820_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	199726
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.48443868099296	34.0	34.0	34.0	34.0	34.0
2	33.58388992920301	34.0	34.0	34.0	34.0	34.0
3	33.718238987412754	34.0	34.0	34.0	34.0	34.0
4	33.491022701100505	34.0	34.0	34.0	34.0	34.0
5	33.5566476072219	34.0	34.0	34.0	34.0	34.0
6	37.18616003925378	38.0	38.0	38.0	36.0	38.0
7	37.26746642900774	38.0	38.0	38.0	36.0	38.0
8	37.14395221453391	38.0	38.0	38.0	36.0	38.0
9	37.03906852387771	38.0	38.0	38.0	36.0	38.0
10-11	36.98409821455394	38.0	37.0	38.0	35.0	38.0
12-13	37.17565815166778	38.0	38.0	38.0	36.0	38.0
14-15	37.206287614031226	38.0	38.0	38.0	36.0	38.0
16-17	37.08976798213553	38.0	38.0	38.0	36.0	38.0
18-19	36.94710002703704	38.0	37.5	38.0	35.5	38.0
20-21	36.975058329911974	38.0	37.5	38.0	35.5	38.0
22-23	36.944982125511956	38.0	37.0	38.0	35.5	38.0
24-25	37.158111612909686	38.0	38.0	38.0	36.0	38.0
26-27	37.061454192243374	38.0	38.0	38.0	36.0	38.0
28-29	36.510491873867196	38.0	37.0	38.0	34.0	38.0
30-31	36.79478635730951	38.0	37.0	38.0	35.0	38.0
32-33	36.86465207334048	38.0	37.0	38.0	35.0	38.0
34-35	36.89131860649089	38.0	38.0	38.0	35.5	38.0
36-37	36.894049848292156	38.0	37.5	38.0	35.5	38.0
38-39	36.03264973013028	38.0	37.0	38.0	33.0	38.0
40-41	36.594194045842805	38.0	37.0	38.0	34.5	38.0
42-43	37.06029009743348	38.0	37.5	38.0	36.0	38.0
44-45	36.8053032654737	38.0	37.0	38.0	35.0	38.0
46-47	36.96098655157566	38.0	37.5	38.0	35.5	38.0
48-49	36.556512422018166	38.0	37.0	38.0	34.0	38.0
50-51	36.749386659723825	38.0	37.0	38.0	34.5	38.0
52-53	36.67040595615994	38.0	37.0	38.0	34.0	38.0
54-55	36.69829416300331	38.0	37.0	38.0	34.5	38.0
56-57	36.536550073600836	38.0	37.0	38.0	34.0	38.0
58-59	36.6432111993431	38.0	37.0	38.0	34.0	38.0
60-61	36.258726955929625	38.0	37.0	38.0	34.0	38.0
62-63	36.068508857134276	38.0	37.0	38.0	33.5	38.0
64-65	36.4973288405115	38.0	37.0	38.0	34.0	38.0
66-67	36.23266875619599	38.0	37.0	38.0	33.0	38.0
68-69	36.31209256681654	38.0	37.0	38.0	34.0	38.0
70-71	35.25778566636292	38.0	36.5	38.0	28.0	38.0
72-73	33.39586483482371	38.0	34.0	38.0	20.0	38.0
74-75	33.12810800797092	38.0	34.0	38.0	11.0	38.0
76	32.9428016382444	38.0	34.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	4.0
23	23.0
24	32.0
25	100.0
26	227.0
27	420.0
28	740.0
29	1276.0
30	2106.0
31	3387.0
32	5093.0
33	8496.0
34	14487.0
35	26714.0
36	34425.0
37	102194.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.60299649857488	15.91620623844755	11.265171589867407	38.21562567311016
2	17.415359041887385	16.63629171965593	42.714518890880505	23.23383034757618
3	16.231737480348077	18.305077956800815	40.43789992289437	25.02528463995674
4	11.885783523427094	27.88470204179726	32.896568298569036	27.332946136206605
5	11.59939116589728	30.88581356458348	44.89200204279864	12.622793226720608
6	25.079358721448386	45.89036980663509	16.54616825050319	12.484103221413337
7	24.492054114136366	26.4422258494137	19.025064338143256	30.04065569830668
8	21.77082603166338	43.784484744099416	15.87675114907423	18.567938075162974
9	20.271271642149745	16.11407628450978	17.01531097603717	46.5993410973033
10-11	21.33372720627259	29.40303215405105	28.694060863382838	20.569179776293524
12-13	21.17450907743609	19.402831879675155	30.184853248951065	29.237805793937692
14-15	19.163003314540923	19.716511620920663	20.857574877582287	40.26291018695613
16-17	27.92726034667494	29.399277009503017	20.570431491142866	22.10303115267917
18-19	27.906231537205972	23.371268638034106	21.46716000921262	27.255339815547302
20-21	19.493456034767632	21.528744379800326	21.494197049958444	37.4836025354736
22-23	19.692979381753002	29.83011726064709	21.047835534682516	29.4290678229174
24-25	27.78870947552885	29.722868944799096	21.16059581925147	21.32782576042058
26-27	27.862922203418684	30.99671550023532	21.92879244565054	19.211569850695454
28-29	19.831419044090403	23.001762414507876	29.63159528554119	27.53522325586053
30-31	26.17711264432272	21.44037331143667	32.09121496450137	20.291299079739243
32-33	20.65354535713928	21.839169662437538	29.99183881918228	27.5154461612409
34-35	19.08339425012267	37.48660665111203	23.79534962899172	19.634649469773592
36-37	27.468882368845314	31.483632576629983	21.81113124981224	19.236353804712454
38-39	19.589838078167087	23.65816168150366	29.97131069565305	26.780689544676207
40-41	18.874858556222023	20.66180667514495	31.675144948579554	28.78818982005347
42-43	27.143390019752207	20.510002528482985	30.753362757198037	21.593244694566767
44-45	18.627770044961597	28.387390725293653	32.554599801728365	20.430239428016385
46-47	26.364619528754396	20.465788129737742	25.730500786076927	27.439091555430938
48-49	25.292650931776535	20.0499684567858	24.234451198141453	30.422929413296217
50-51	18.5938556298355	20.301459726082275	32.73867648163449	28.36600816244773
52-53	16.660155858725485	20.601197988661177	40.49847747260452	22.240168680008814
54-55	16.143615689024696	20.66147372639383	33.79126383810049	29.403646746480987
56-57	24.29097066197779	20.648326958769474	25.691120445443	29.369581933809734
58-59	16.7745166783532	20.57046979865772	39.32585395171792	23.32915957127116
60-61	24.56865249292565	28.459419527708913	25.34269902085994	21.629228958505497
62-63	16.517712932055563	39.16577561532625	21.986973538905346	22.32953791371284
64-65	16.068495483043886	40.018628688179795	21.181118923141636	22.73175690563468
66-67	16.146747636636825	39.93338759156076	20.516371376698178	23.40349339510424
68-69	16.06410519621638	40.25177388421806	20.300896831795214	23.383224087770337
70-71	16.88971123969384	38.529332969451445	20.607565905602694	23.97338988525202
72-73	17.796663219544932	34.43424218826293	21.118670295743016	26.65042429644913
74-75	18.19704556835253	32.70455683525288	22.2336004006009	26.864797195793688
76	18.112302384159683	33.341679477998724	21.749385306445394	26.796632831396195
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	0.5
11	0.0
12	0.0
13	1.5
14	9.0
15	9.5
16	7.0
17	10.0
18	29.5
19	61.0
20	88.0
21	103.0
22	141.5
23	213.5
24	356.5
25	466.0
26	573.5
27	712.0
28	883.5
29	1024.0
30	1195.0
31	1622.5
32	2212.5
33	2546.0
34	2849.5
35	3514.5
36	4316.5
37	4757.0
38	5372.5
39	10481.5
40	17352.0
41	18986.0
42	18243.0
43	16474.0
44	13599.5
45	11752.5
46	11011.0
47	10800.0
48	10100.0
49	9189.0
50	8767.0
51	8411.0
52	7260.0
53	6237.0
54	6009.0
55	5415.0
56	4384.5
57	3578.0
58	3208.0
59	2912.0
60	2458.5
61	2080.5
62	1860.0
63	1783.0
64	1569.0
65	1401.0
66	1261.5
67	1153.0
68	1134.0
69	1017.5
70	930.5
71	941.0
72	898.0
73	722.5
74	648.0
75	706.0
76	604.0
77	502.0
78	415.5
79	329.0
80	309.0
81	276.0
82	226.5
83	190.0
84	145.5
85	79.0
86	55.5
87	54.0
88	43.5
89	24.0
90	12.5
91	8.0
92	6.0
93	4.0
94	6.0
95	5.5
96	1.0
97	0.5
98	0.5
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04656379239558195
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.006859397374403E-4
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	7.510289096061604E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.015521264131860649
52-53	0.029039784504771536
54-55	0.048065850214794266
56-57	0.05232168070256251
58-59	0.03304527202267106
60-61	0.03054184232398386
62-63	0.027788069655427936
64-65	0.01702332195107297
66-67	0.03179355717332746
68-69	0.011515776613961127
70-71	0.013768863342779606
72-73	0.021279152438841212
74-75	0.01301783443317345
76	0.01652263601133553
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	199726.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.991237996054593
#Duplication Level	Percentage of deduplicated	Percentage of total
1	39.96874624353889	9.988684497761934
2	14.98577553391834	7.4902616584721065
3	13.10453980847057	9.824960195467792
4	10.351805104780222	10.348177002493417
5	7.0240814200424735	8.777024523597328
6	4.531794686861402	6.79530957411654
7	2.7026485555154864	4.727977328940649
8	1.676884240894338	3.3525930524819003
9	1.2060744480506471	2.7127164214974515
>10	4.361501783066875	17.665702011756107
>50	0.05809993188283848	0.9487998558024494
>100	0.016027567415955443	0.900734005587655
>500	0.006010337780983291	0.8982305758889679
>1k	0.004006891853988861	2.6100758038512764
>5k	0.0	0.0
>10k+	0.0020034459269944304	12.95875349228443
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT	25882	12.95875349228443	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG	3374	1.6893143606741237	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC	1839	0.9207614431771528	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC	649	0.32494517488959873	No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT	639	0.31993831549222435	Illumina PCR Primer Index 9 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT	506	0.2533470855071448	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCGT	465	0.23281896197790974	No Hit
CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC	268	0.13418383184963398	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATATCGTATGCCGT	215	0.10764747704354967	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAGCTCGTATGCCGT	215	0.10764747704354967	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0015020578192123208	0.0
2	0.0	0.0	0.0	0.0015020578192123208	0.0
3	0.0	0.0	0.0	0.0015020578192123208	0.0
4	0.0	0.0	0.0	0.004005487517899522	0.0
5	0.0	0.0	0.0	0.008511660975536486	0.0
6	0.0	0.0	0.0	0.008511660975536486	0.0
7	0.0	0.0	0.0	0.008511660975536486	0.0
8	0.0	0.0	0.0	0.008511660975536486	0.0
9	0.0	0.0	0.0	0.011015090674223687	0.0
10	0.0	0.0	0.0	0.012517148493436008	0.0
11	0.0	0.0	0.0	0.012517148493436008	0.0
12	0.0	0.0	0.0	0.014019206312648328	0.0
13	0.0	0.0	0.0	0.014019206312648328	0.0
14	0.0	0.0	0.0	0.014019206312648328	0.0
15	0.0	0.0	0.0	0.01502057819212321	0.0
16	0.0	0.0	0.0	0.01502057819212321	0.0
17	0.0	0.0	0.0	0.01702332195107297	0.0
18	0.0	0.0	0.0	0.018525379770285292	0.0
19	0.0	0.0	0.0	0.018525379770285292	0.0
20	0.0	0.0	0.0	0.021028809468972492	0.0
21	0.0	0.0	0.0	0.023031553227922254	0.0
22	0.0	0.0	0.0	0.025534982926609454	0.0
23	0.0	0.0	0.0	0.031543214203458736	0.0
24	0.0	0.0	0.0	0.03404664390214594	0.0
25	0.0	0.0	0.0	0.03805213142004546	0.0
26	0.0	0.0	0.0	0.042558304877682425	0.0
27	0.0	0.0	0.0	0.07860769253877813	0.0
28	0.0	0.0	0.0	0.11816188177803591	0.0
29	0.0	0.0	0.0	0.15821675695703113	0.0
30	0.0	0.0	0.0	0.19176271491943964	0.0
31	0.0	0.0	0.0	0.2473388542302955	0.0
32	0.0	0.0	0.0	0.3034156794808888	0.0
33	0.0	0.0	0.0	0.36449936412885653	0.0
34	0.0	0.0	0.0	0.4441084285471095	0.0
35	0.0	0.0	0.0	0.5697805994212071	0.0
36	0.0	0.0	0.0	0.7900824129056808	0.0
37	0.0	0.0	0.0	1.160089322371649	0.0
38	0.0	0.0	0.0	1.647757427675916	0.0
39	0.0	0.0	0.0	2.240068894385308	0.0
40	0.0	0.0	0.0	3.0176341587975526	0.0
41	0.0	0.0	0.0	4.033025244585081	0.0
42	0.0	0.0	0.0	5.133032254188238	0.0
43	0.0	0.0	0.0	6.165947347866577	0.0
44	0.0	0.0	0.0	7.166317855461983	0.0
45	0.0	0.0	0.0	8.098595075253098	0.0
46	0.0	0.0	0.0	9.080440203078217	0.0
47	0.0	0.0	0.0	9.891050739513133	0.0
48	0.0	0.0	0.0	10.767751819993391	0.0
49	0.0	0.0	0.0	11.587875389283319	0.0
50	0.0	0.0	0.0	12.395982496019547	0.0
51	0.0	0.0	0.0	13.256661626428206	0.0
52	0.0	0.0	0.0	14.175921011786148	0.0
53	0.0	0.0	0.0	15.228863543053984	0.0
54	0.0	0.0	0.0	16.20570181148173	0.0
55	0.0	0.0	0.0	17.03533841362667	0.0
56	0.0	0.0	0.0	17.732293241741186	0.0
57	0.0	0.0	0.0	18.470304316914174	0.0
58	0.0	0.0	0.0	19.17827423570291	0.0
59	0.0	0.0	0.0	19.856202998107406	0.0
60	0.0	0.0	0.0	20.50909746352503	0.0
61	0.0	0.0	0.0	21.11142264902917	0.0
62	0.0	0.0	0.0	21.76882328790443	0.0
63	0.0	0.0	0.0	22.37465327498673	0.0
64	0.0	0.0	0.0	23.031052541982515	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGCGTA	15	0.0022245084	69.98173	24
GCTTGAC	35	2.9380863E-7	60.07458	56
TACATCG	25	2.3893207E-4	55.985374	39
CATCGCG	25	2.3893207E-4	55.985374	41
ATCTCGT	3535	0.0	53.15443	37
TCTCGTA	3550	0.0	53.126972	38
GATCTCG	3545	0.0	53.00449	36
AGATCTC	3565	0.0	52.805283	35
CTCGTAT	3765	0.0	52.788338	39
GGTCGAG	20	0.006930215	52.525745	45
CTCCGTA	20	0.0069508455	52.486294	22
CATGCTG	20	0.0069508455	52.486294	41
TGGACTA	20	0.0069508455	52.486294	5
CGGCCGT	20	0.0069508455	52.486294	13
CGGGCAA	20	0.0069508455	52.486294	33
AATGTAC	20	0.0069508455	52.486294	26
TCTATAG	20	0.0069508455	52.486294	32
TCACGTG	20	0.0069508455	52.486294	15
CGTATGC	3830	0.0	52.349255	41
TCGTATG	3825	0.0	52.326202	40
>>END_MODULE